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Citations for Roche :
1851 - 1900 of 8155 citations for 6 AMINO 4H 7H 1 3 DITHIINO 5 4 D PYRIMIDIN 8 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... fresh sinonasal tissue samples underwent enzymatic digestion with a mixture of 25mg/mL collagenase D (Roche Diagnostics GmbH ...
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bioRxiv - Cell Biology 2024Quote: ... D-Mel (d.mel-2) cells were transfected using X-tremeGene HP DNA transfection reagent (#06366236001, Roche). Two days after transfection ...
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bioRxiv - Molecular Biology 2020Quote: Frozen extracted mitochondria were lysed by sonication in 300 μl of lysis buffer (8 M urea, 50 mM ammonium bicarbonate, 1X protease inhibitors (Complete mini EDTA-free mixture [Roche Applied Science] ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were harvested with a cell scraper and lysed by adding 2 x TNI buffer 8 containing complete Ultra protease inhibitor (Roche) and Halt phosphatase inhibitor (Pierce) ...
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bioRxiv - Plant Biology 2020Quote: ... Cells were then harvested and resuspended in 8 mL of Native Purification Buffer (50 mM NaH2PO4, 500 mM NaCl) supplemented with 8 mg lysozyme and a protease inhibitor tablet (Roche). The suspension was then incubated on ice for thirty minutes ...
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bioRxiv - Cell Biology 2019Quote: 10 heads from animals at 8 DAE were resuspended in 100 μl of Hepes/NaOH 50mM with proteases and phosphatases inhibitors (Roche), samples were disrupted using a mechanical tip following two rounds of sonication of 10 sec each ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were then pelleted again and resuspended in 150 μL of Lysozyme buffer (150 mM NaCl, 30 mM Tris–HCl pH 8, 10 mM EDTA, cOmplete protease inhibitor (Roche), 1 mg/ml lysozyme ...
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bioRxiv - Cell Biology 2021Quote: ... air-dried and resuspended in 50 µl of 8 M urea in 50 mM triethylammoniumbicarbonate including protease inhibitor cocktail (Roche). Afterwards proteins were reduced with 5 mM dithiothreitol for 1h at 25°C and alkylated with 40 mM chloroacetamide for 30 min in the dark ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested and resuspended in Wash Buffer (150mM NaCl, 100mM Tris-HCl, pH 8) and a Complete protease inhibitor tablet was added (Roche). Cells were lysed by sonication (25% amplitude 1 sec on ...
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bioRxiv - Cell Biology 2021Quote: U2OS cells were seeded into 8-well Imaging Chamber CG (Zell-Kontakt) and transfected 48-72 h prior to imaging using XtremeGene HP (Roche) according to the manufacture’s instructions ...
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bioRxiv - Genetics 2020Quote: ... and resuspended in 300 μL of Nuclei Lysis Buffer (Tris-HCl pH 8 50 mM, EDTA 10 mM, SDS 0.5% and protease inhibitor (Roche-04693132001). Nuclei were then sonicated (Diagenode bioruptor plus sonicatore ...
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bioRxiv - Microbiology 2020Quote: ... the culture medium was replaced with serum-free medium 8 h prior to addition of IFN-γ (1000 IU; Roche). In experiments with Tofacitinib ...
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bioRxiv - Plant Biology 2021Quote: ... The adaptor-ligated double-stranded cDNAs were amplified by PCR for 8 cycles with the Kapa HiFi polymerase (Roche, CA) to reduce the likeliness of multiple identical reads due to preferential amplification ...
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bioRxiv - Cancer Biology 2019Quote: ... 2 μl of the purified cDNA was mixed with 8 μl of a qPCR mixture [1.25x KAPA HiFi HotStart Readymix (KAPA Biosystems), 0.625 μM PCR lnPE1 primer ...
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bioRxiv - Neuroscience 2019Quote: ... Neurons were washed three times with PBS and harvested directly in 8 M Urea lysis buffer supplemented with phosphatase inhibitor (PhosSTOP, Roche) and protease inhibitor (cOmplete mini EDTA-free ...
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bioRxiv - Neuroscience 2019Quote: ... Harvested cells were washed once with PBS and resuspended in His-Trap binding buffer (50 mM Tris, pH 8; 150 mM NaCl; 20 mM imidazole; protease inhibitor cocktail (Roche) and 50 μM NBQX to displace glutamate and promote dimerization) ...
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bioRxiv - Cell Biology 2019Quote: ... washed in PBS, and resuspended in Lysis Buffer (Tris 10 mM pH 8, 2 mM EDTA) supplemented with Complete protease inhibitor (Roche, Merck ...
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bioRxiv - Biophysics 2020Quote: The HEK293 cell pellet was resuspended in 25 mL IEX buffer A (50 mM Tris, pH 8) with protease inhibitors (cOmplete, EDTA-free cocktail, Roche) and sonicated 20 s on ...
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bioRxiv - Genomics 2022Quote: ... Each ligation product was index-amplified using unique dual 8-bp indexing primers for eight cycles with KAPA HiFi polymerase (Roche).
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bioRxiv - Genomics 2022Quote: ... The cell pellet was lysed in cell lysis buffer (20 mM Tris-HCl pH 8, 85 mM KCl, 0.5% NP-40) supplemented with 1X protease inhibitors (Roche, 11836170001) on ice for 20 minutes then spun at 5000 rpm for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in 50 ml lysis buffer (Hepes 50 mM pH 8, 500 mM NaCl, 10 mM Imidazole, benzonase, EDTA-free protease inhibitor cocktails (ROCHE)) at 4°C and sonicated ...
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bioRxiv - Neuroscience 2023Quote: ... The cell pellet was resuspended (20 mL/L culture volume) in lysis buffer (20 mM Tris pH 8, 150 mM NaCl, and protease inhibitor cocktail, Roche). These resuspended cells were disrupted using sonication (QSonica sonicator ...
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bioRxiv - Microbiology 2023Quote: ... A total amount of 1010 bacteria were pelleted at 4°C and resuspended in 1 mL of buffer A (20 mM Tris-HCl pH 8, 200 mM MgCl2) supplemented with protease inhibitor cocktail (PIC, Roche) and lysozyme to a final concentration of 0.5 mg/mL ...
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bioRxiv - Synthetic Biology 2023Quote: ... The second strand synthesis reaction was performed with ∼50 ng (IDT) or ∼8 ng (Twist) of template DNA and KAPA HiFi polymerase (Roche) with the annealing temperature at 53°C and extension time of 30 sec for 2 (IDT ...
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Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme researchbioRxiv - Biochemistry 2023Quote: ... The bacterial pellets were dispersed in lysis buffer of 8 M urea in 100 mM ammonium bicarbonate and protease inhibitor cocktail (CoMplete, (Roche)) ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed in lysis buffer (2% SDS in 50 uM Tris-HCL pH.8, supplemented with EDTA-free protease inhibitor, Roche) at cell concentration of 20×106/mL and incubated at 95 °C for 15 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... the cell pellets resuspended in lysis buffer (purification buffer 50 mM Tris pH 8, 50 mM NaCl, 2 mM DTT; + cOmplete EDTA-free Protease Inhibitor (Roche)) and lysed by sonication ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria were lysed in equilibration buffer (20 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8) supplemented with lysozyme and anti-protease cocktail (Roche), followed by sonication ...
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bioRxiv - Molecular Biology 2023Quote: ... a total of 5 μg of plasmid and dsRNA were transfected into BmN4 cells (8 × 105 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche) every 3 days four times ...
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bioRxiv - Pathology 2024Quote: ... The proteins were redissolved in 8 M Urea/50 mM TEAB buffer containing protease- and phosphatase inhibitors (EDTA free cOmpleteTM, PhosSTOPTM Roche) and treated 30 minutes at 37 °C with 50 units/100 µg tissue benzonase (Sigma Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... 100 synchronized young adult worms (24h post-L4 stage) were picked into 30 μl of 1XTE (Tris-EDTA Buffer, pH=8) containing 1X complete protease inhibitor cocktail (Roche) and flash-frozen in liquid nitrogen ...
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bioRxiv - Immunology 2022Quote: ... and pLenti-Luc-GFP (6 µg) into HEK293T cells using X tremeGENE HP DNA Transfection Reagent (Roche) according to the manufacturer′s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... The Th17 cultured cells were stimulated in presence of IL-6 (20ng/ml, Roche, Cat# 11138600 001); IL-1β (10ng/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Vector DNA was transiently transfected into human embryonic kidney (HEK) cells using Fugene-6 (Roche Molecular Biochemicals) transfection reagent ...
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bioRxiv - Immunology 2023Quote: ... Serum IL-6 and CRP levels were measured using in vitro diagnostic methods validated at PPD (Roche Cobas ...
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bioRxiv - Plant Biology 2020Quote: ... Blots were hybridized with a digoxygenin (DIG)-labeled ZmPEPC promoter-specific probe synthesized using primers (Fwd 5’-TCCCGAGTTCCTAACCACAG; and Rev 5’-GTGGCTGAGGCTTCTTTTTG) and the PCR DIG Probe Synthesis Kit (Roche Diagnostics, Switzerland). The signals were detected by CDP-Star (Roche Diagnostics ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1 mM CaCl2, 5 mM EGTA, 50 mM sucrose, 0.1% Triton X-100, 1/2 tablet of cOmplete inhibitor [Roche], and 2.5 U/mL Benzonase [Novagen]), lysed by sonication ...
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bioRxiv - Neuroscience 2020Quote: ... and Laminin (5 µg/mL, Roche, Basel, Switzerland; 11243217001) coated plates ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 5 mg/mL liberase DL (Roche, 05401160001) and 2.5 mg/mL DNase (Roche 10104159001 ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM EDTA) with protease and phosphatase inhibitors (Roche) and incubated on ice for 20 min ...
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bioRxiv - Genetics 2021Quote: ... 5 ng of cDNA on a LightCycler96 system (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: BSA Protein Assay Kit (A8020-5, Roche, Basel, Switzerland) was applied to measure the protein concentration after lysing BC cells with RIPA buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 μl 5X KAPA HiFi Fidelity buffer (KAPA Biosystems), 0.75 μl of each for forward and reverse primer mix (10 mM) ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 mM EDTA) containing proteases and phosphatases inhibitors (Roche MiniProtease Inhibitor cocktail ...
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bioRxiv - Developmental Biology 2019Quote: ... 5 mM NaF) plus protease inhibitors (Roche Mini cOmplete) using a Dounce homogenizer and a Wheaton overhead stirrer ...
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bioRxiv - Genetics 2021Quote: ... 5 mM β-mercaptoethanol supplemented with protease inhibitors (Roche), 1mM PMSF ...
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bioRxiv - Plant Biology 2021Quote: ... 5 mM β-mercaptoethanol and protease inhibitor cocktail (Roche) for 1 h at 4℃ ...
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bioRxiv - Cancer Biology 2022Quote: ... 5% glycerol with protease inhibitors and phosphatase inhibitors (Roche)] ...
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bioRxiv - Biochemistry 2022Quote: ... 5% glycerol and supplemented with protease inhibitor cocktail (Roche)) for 60 minutes on ice ...
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bioRxiv - Plant Biology 2021Quote: ... 5 mM 2-mercaptoethanol and protease inhibitor cocktail (Roche). The samples were centrifuged at 16,000g for 45 min at 4°C ...