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Citations for Roche :
1801 - 1850 of 8155 citations for 6 AMINO 4H 7H 1 3 DITHIINO 5 4 D PYRIMIDIN 8 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... PHB variant complexes were captured using 25 μl Protein A Dynabeads and washed 4x with freshly prepared Wash Buffer (50mM HEPES pH 8, 450mM NaCl, 0.1% NP-40, 1x cOmplete ULTRA protease inhibitor - EDTA (Roche), 1mM PMSF ...
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bioRxiv - Plant Biology 2022Quote: ... 4-day-old tmk1-1 roots expressing TMK1::TMK1-FLAG were grounded to powder in liquid nitrogen and homogenized in ice-cold sucrose buffer (20 mM Tris–HCl pH 8, 0.33 M sucrose, Complete EDTA-free Protease Inhibitor cocktail (Roche), PhosSTOP phosphatase inhibitor cocktail (Roche)) ...
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bioRxiv - Physiology 2023Quote: ... approximately 15 mg of pulverized mouse intestinal tissue was solubilized in ice-cold urea Lysis buffer (8 M urea in 50 mM Tris, pH 8.0, 40 mM NaCl, 2 mM MgCl2, 1x Roche cOmplete ULTRA EDTA-free protease inhibitor mini tablet ...
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bioRxiv - Physiology 2023Quote: ... The adaptor-ligated double-stranded cDNAs were amplified by PCR for 8 cycles with the Kapa HiFi polymerase (Roche). The final libraries were quantitated with Qubit (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell pellets were lysed with 200 μl buffer containing 8 M urea and 200 mM EPPS (pH at 8.5) with protease inhibitor (Roche) and phosphatase inhibitor cocktails 2 and 3 (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... containing 0.1 mM CaCl2 and 1.0 mM MgCl2 and lysed in 8 urea with Complete protease inhibitor (Roche 11245200) and/or Halt phosphatase inhibitor (Pierce A32957 ...
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bioRxiv - Cell Biology 2024Quote: ... or ice-cold 8% (v/v) paraformaldehyde solution/ 0.2% (v/v) glutaraldehyde in vPBS containing EDTA-free protease inhibitor (Roche) was added to the resuspended cells ...
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bioRxiv - Molecular Biology 2024Quote: Ni-NTA Magnetic Agarose Beads were incubated with 1.5µg of recombinant His-TIRR(8) in binding buffer (50mM Tris pH 7.4, 150mM NaCl, 5mM MgCl2, 10% Glycerol, Protease inhibitors (EDTA free, Roche)) for 1 hour at 4°C on a wheel ...
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bioRxiv - Bioengineering 2024Quote: ... The adaptor-ligated DNA was amplified by PCR for 8 cycles with the Kapa HiFi polymerase (Roche, Indianapolis, IN). The final libraries were quantitated on Qubit and the average size determined on the AATI Fragment Analyzer (Agilent Technologies ...
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bioRxiv - Cell Biology 2024Quote: Samples were lysed in buffer containing 8 M urea and 200 mM EPPS (pH at 8.5) with protease inhibitor (Roche) and phosphatase inhibitor cocktails 2 and 3 (Sigma) ...
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bioRxiv - Plant Biology 2020Quote: ... glycerol 25%, KCl 20 mM, EDTA 2 mM, MgCl2 2.5 mM, Sucrose 250 mM, DTT 5 mM, protease inhibitor Roche™, 1 tablet/ 50 mL) at a ratio of 1:3 (w/v) ...
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bioRxiv - Developmental Biology 2020Quote: ... DIGprobes were detected with antibodies in MABT containing 5% horse serum appropriate for NBT/BCIP in situ hybridization (Roche anti-DIG-AP 1:1000) or for FISH (Roche anti-DIG-POD 1:1000) ...
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bioRxiv - Biochemistry 2020Quote: ... Cell pellets were resuspended in lysis buffer (50 mM HEPES-NaOH, pH 7.5, 500 mM NaCl, 5% v/v glycerol, 1 mM TCEP-NaOH, Roche protease inhibitor cocktail EDTA-free) at the ratio of 50 ml / L equiv ...
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bioRxiv - Molecular Biology 2021Quote: ... and homogenized in 5 volumes of ice-cold PBS containing 1% NP-40 and Complete® protease inhibitor cocktail tablets (Roche Diagnostics, Basel, Switzerland) using 2×10 clockwise strokes with 5 s rest time ...
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bioRxiv - Plant Biology 2022Quote: ... mCIT tagged proteins were revealed by using respectively GFP monoclonal antibody (anti-GFP mouse monoclonal, Roche; at 1/1000 in 5 % milk over-night) as primary antibodies and anti-mousse IgG-HRP conjugated secondaries antibodies (Mouse IgG ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5 μL of each forward and reverse primers (5 pM) and 5 μL of 2X FastStart SYBR Green master mix (# 6924204001, Roche, USA). The PCR cycling condition set in Light Cycler 480 instrument (Roche Diagnostics ...
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bioRxiv - Systems Biology 2023Quote: ... HepG2s were washed once with 1X PBS then lysed in SDS lysis buffer (50 mM Tris HCl/2% SDS/5% glycerol/5 mM EDTA/1mM NaF/dH2O) supplemented with cOmplete Protease Inhibitor Cocktail Tablets (Roche #11836170001), Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich® #P5726) ...
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bioRxiv - Neuroscience 2023Quote: ... 500 μl papain inhibitor solution containing 5 mg/ml BSA (Carl Roth, 8076.4) and 5 mg/ml Trypsin inhibitor (Sigma-Aldrich/Roche, 10109878001) in PBS was added ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated with 4-Nitroblue tetrazolium chloride (NBT, Roche) and 5-bromo-4-chloro-3-indolyl-phosphate (BCIP ...
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bioRxiv - Biophysics 2020Quote: ... 4 mM digitonin and EDTA-free protease inhibitor tablets (Roche). Insoluble material was removed by ultracentrifugation at 100,000 g for 30 min ...
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bioRxiv - Physiology 2019Quote: ... 4 Pefabloc (Roth) and Complete® proteinase inhibitor cocktail (Roche). Cell debris were removed by centrifugation at 1000 g for 10 min and the supernatant was ultracentrifuged at 269,000 g for 30 min ...
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bioRxiv - Physiology 2019Quote: ... 4 Pefabloc (Roth) and Complete® proteinase inhibitor cocktail (Roche). Protein concentration of the membrane fractions was determined by BCA.
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bioRxiv - Physiology 2020Quote: ... 4 µL of 0.25 µg/µL trypsin (Roche, sequencing grade) was added to each sample and left overnight at 37 °C.
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bioRxiv - Immunology 2019Quote: ... grade II and 4 mg collagenase A (Roche Applied Science) and placed in a 37°C water bath for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 ul of 20 mg/ml proteinase K (Roche 3115801) was added ...
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bioRxiv - Neuroscience 2024Quote: ... counterstained using the Red Counterstain II kit (4 min, Roche), removed from the automated system and rinsed with distilled water ...
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bioRxiv - Biochemistry 2020Quote: ... blocked in 3% BSA and probed with anti-HA (clone 12CA5, Roche), anti-cMyc (clone 9E10 ...
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bioRxiv - Neuroscience 2020Quote: ... Following resuspension in (3 ml) homogenization medium containing protease inhibitor (Roche Diagnostics), the cells were cracked open using a ball bearing homogenizer with a 0.2507-inch bore and 0.2496-inch diameter ball ...
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bioRxiv - Systems Biology 2020Quote: ... The tissue was digested with Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Blocking was then performed with DBPS containing 3% BSA (Roche, Cat#3116956001) and 1% Chicken serum (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... and 3 µl 10 mM dNTP (KAPA HiFi Kits from Roche, KK2502) were added to the annealed oligos ...
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bioRxiv - Developmental Biology 2024Quote: ... Beads were washed 3 times with lysis buffer containing proteinase inhibitors (Roche) and 7 times with lysis buffer without proteinase inhibitors ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei lysates (in Nuclear Buffer Lysis 3, supplemented with protease (Roche, 11697498001) and phosphatase inhibitors (1 mM Na3O4V (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... 50 mM Tris-HCl (pH 8.0) and protease inhibitor (one tablet of Roche cOmplete™ mini per 10 ml lysis buffer) [22] ...
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bioRxiv - Developmental Biology 2022Quote: ... 50mM Tris-HCl [pH 7.2], 250mM NaCl, 2mM EDTA, phosphatase inhibitor mixture I and II [Calbiochem] and one tablet of Minicomplete [Roche] per 10ml) on ice ...
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bioRxiv - Physiology 2021Quote: ... at time zero (T0) peripheral glucose level was measured at the tail using a One-Touch ultra-glucometer (AccuChek Sensor, Roche Diagnostics) and at T15 ...
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bioRxiv - Microbiology 2021Quote: ... 150 mM NaCl, 20 mM Imidazole pH 8.0, one tablet of EDTA-free protease inhibitor [Roche; Product # COEDTAF-RO], DNaseI [Roche; Product #11284932001]) and lysed and centrifuged for 30 minutes at 18,000 x g at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... One step real time RT-PCR was performed using 100 ng of total RNA and KAPA SYBR FAST One-Step qRT-PCR Kits (Kapa Biosystems) according to the directions provided by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: ... 4 mM sodium dithionite) supplemented with 0.2 mg/mL bovine pancreatic deoxyribonuclease I and one cOmplete EDTA-free protease inhibitor tablet (Roche, Basel, Switzerland). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... The cell pellet was resuspended in chilled lysis buffer (Bugbuster) containing one dissolved cOmplete™ EDTA-free Protease Inhibitor cocktail tablet (Roche). The cell lysates were clarified by centrifugation (11,000 × g ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were centrifuged and the pellet was resuspended in Resuspension Buffer (25 mM Tris HCl pH 7.5, 250 mM NaCl) supplemented with one tablet of cOmplete protease inhibitor (Roche, Cat # 04693159001). Cells were then lyzed using a high-pressure Emulsiflex C5 homogenizer (Avestin) ...
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bioRxiv - Physiology 2024Quote: Blood glucose levels were measured immediately in one drop of blood after the heart puncture with the Blood Glucose System Accu-Chek Performa (Roche Diagnostics).
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bioRxiv - Physiology 2024Quote: ... one lobe of dissected adipose tissue was homogenized with a Teflon pestle in RIPA buffer containing protease (cOmpleteTM Mini, Roche, Germany) and phosphatase (5 mM Na fluoride ...
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bioRxiv - Cell Biology 2022Quote: ... Brain tissue was minced with scissors and incubated in 4,000 U/ml of collagenase D (Roche) at 37°C for 30 minutes ...
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bioRxiv - Immunology 2019Quote: ... Lungs were finely minced and digested in complete RPMI supplemented with collagenase D (0.1mg/mL; Roche) and DNase I (20U/mL ...
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bioRxiv - Plant Biology 2021Quote: ... collected and the enzymatic assay was performed using red-β-D-galactopyranoside (CPRG, Roche Applied Science) as substrate as described (Yeast Protocols Handbook ...
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bioRxiv - Immunology 2022Quote: ... CNS was minced with a razor blade and digested with collagenase D (2.5mg/ml, Roche Diagnostics) at 37°C for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... inguinal adipose tissue was digested in a PBS buffer containing 10 mg/mL collagenase D (Roche), 2.4 U/mL dispase II ...
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bioRxiv - Immunology 2020Quote: The vaccine-draining popliteal LN was incubated with 3ml of filtered 1mg/ml collagenase D (Roche) for 30 minutes at 37 C’ ...
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bioRxiv - Immunology 2023Quote: ... tumors were cut into small pieces and enzymatically digested using 1mg/ml Collagenase D (#11088882001, Roche), and 0.1mg/ml DNase I (#101104159001 ...