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Citations for Roche :
1951 - 2000 of 8155 citations for 6 AMINO 4H 7H 1 3 DITHIINO 5 4 D PYRIMIDIN 8 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: GGCAGCGCTACCATAACGGA-3’) (11) by RT-qPCR was performed using LightCycler 480 II (Roche). GAPDH mRNAs were used as a loading control (14) ...
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bioRxiv - Developmental Biology 2023Quote: ... After a brief enzymatic treatment with a cocktail of Liberase Blendzyme 3 (Roche), trypsin B (BI) ...
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bioRxiv - Microbiology 2019Quote: ... after which they were centrifuged through a TE Select-D G-25 spin column (Roche Applied Science) to remove unincorporated [32P] dATP ...
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bioRxiv - Cell Biology 2020Quote: ... and LacZ activity was measured using a liquid β-galactosidase assay employing chlorophenolred-ß-D-galactopyranoside (Roche) as a substrate.
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bioRxiv - Immunology 2019Quote: ... liver tissue was minced and digested in an enzyme cocktail 0.625 mg/ml collagenase D (Roche, 11088882001), 0.85 mg/ml collagenase V (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... EDTA-free protease inhibitor cocktail and isopropyl-β-D-thiogalactoside (IPTG) were purchased from Roche (Basel, Switzerland). A polyclonal rabbit-anti-uPA antibody (ab2412 ...
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bioRxiv - Cell Biology 2021Quote: ... and then with 15-20 ml of 37 °C EBS containing collagenase D (0.1 U/ml) (Roche). Damaged livers (48h-72h after APAP injection ...
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bioRxiv - Microbiology 2022Quote: ... and enzymatic digestion was performed in Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 0.2mg/mL collagenase D (Roche), 0.01mg/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... a mix of enzymes was used (collagenase V, Sigma, C9263-1G, 0.85 mg/ml; Collagenase D, Roche, 11088882001 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumors were weighed and dissociated with digestion media (collagenase D and DNase I (both Roche, Sigma-Aldrich) in PBS ...
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bioRxiv - Immunology 2020Quote: ... placed in 10 mL of pre-warmed RPMI + 10% FBS + 1.5 mg/mL Collagenase D (Roche 11088866001) + 0.05 mg/mL Collagenase V (Sigma C9263 ...
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bioRxiv - Bioengineering 2021Quote: ... then digested at 37°C for 45 min in IMDM with 3.5 mg/mL collagenase D (Roche) before being passed through a 70μm filter ...
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bioRxiv - Molecular Biology 2023Quote: ... in the smallest volume of ice-cold 1X PBS - 0,6% D-glucose - 5mg/ml DNaseI (Roche #10104159001) solution ...
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bioRxiv - Developmental Biology 2023Quote: ... in the smallest volume of ice-cold "1X PBS - 0,6% D-glucose - 5mg/ml DNaseI (Roche #10104159001)" ...
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bioRxiv - Immunology 2023Quote: ... after cutting SVF into small pieces and incubation in a 2 mg/ml collagenase D solution (Roche) at 37°C in a water bath for 45 min ...
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bioRxiv - Immunology 2023Quote: ... plus 2% fetal calf serum (Dutscher)) supplemented with 50μg/mL DNAse and 10μg/mL collagenase D (Roche).
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bioRxiv - Developmental Biology 2021Quote: ... Signals were developed using 4-nitro blue tetrazolium chloride (NBT, Roche) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.25% Triton supplemented with protease inhibitor at 4□°C (Roche, 04693159001) for 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... supplemented with protease inhibitors (4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (Roche), benzamidine ...
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bioRxiv - Cell Biology 2022Quote: ... 4 mM MgSO4) containing Complete Inhibitor Protease Cocktail (Roche, Indianapolis, IN), lysozyme and DNAse ...
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bioRxiv - Evolutionary Biology 2022Quote: ... at 4°C overnight and subsequently developed with NBT/BCIP (Roche). For FISH ...
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bioRxiv - Physiology 2020Quote: ... containing 0.2 mg 4-(2-aminoethyl)-benzene-sulfonyl fluoride (AEBSF, Roche). Serum from blood samples was obtained by centrifugation at 3,000 rpm for 15 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and 4 complete-EDTA protease-inhibitor tablets per 500 mL (Roche). After thawing ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA (4 ng) and LightCycler 480 SYBR Green I Master (Roche) in a final volume of 10 μl ...
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bioRxiv - Neuroscience 2023Quote: ... 4% BlockAce (DS Pharma Biomedical) and 0.5× Blocking reagent (Roche Diagnostics)] for 1 hr at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunohistochemistry was performed on 4 μm sections using the BenchmarkUltra (Roche), anti-hCD45 (M0701 ...
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bioRxiv - Neuroscience 2024Quote: ... and detection with 4-Nitro blue tetrazolium chloride (NBT; 11383213001, Roche) and 5-bromo-4-chloro-3-indolyl-phosphate (BCIP ...
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bioRxiv - Cell Biology 2020Quote: ... cells were collected via centrifugation and resuspended in lysis buffer (40mM Tris pH 8, 300mM NaCl, 10% glycerol, 10mM imidazole, 1mM DTT, 1mM PMSF and 1x Roche PIC). Cells were lysed by sonication and after clearing the lysate ...
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bioRxiv - Molecular Biology 2021Quote: ... resuspended in lysis buffer (100 mM Tris pH 8, 300 mM NaCl, 5ml Mg2Cl, 1mM ß-mercaptoethanol and protease inhibitor tablets (complete tablets, Roche) and lysed by sonication ...
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bioRxiv - Evolutionary Biology 2022Quote: ... were ligated to the mate pair fragments and 8 cycles of PCR was used to enrich for the final library (KAPA Biosystems). The prepared libraries were quantified using KAPA Biosystems’ next-generation sequencing library qPCR kit and run on a Roche LightCycler 480 real-time PCR instrument ...
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bioRxiv - Immunology 2019Quote: ... Cells were lysed (10mM Tris-HCl [pH 8], 0.25% Triton X-100, 10mM EDTA, 100mM NaCl, Roche 1X Complete Protease Inhibitor) and nuclei were collected and re-suspended in 300μl SDS lysis buffer (1% SDS ...
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bioRxiv - Genomics 2019Quote: We amplified 5 ng of the HSS library with the following primers: STARR-Seq-AG-f and STARR-Seq-AG-r (Supplemental Table 8) using KAPA HiFi 2x Readymix (Kapa Biosystems) with a 65 °C annealing temperature and 30 s extension ...
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bioRxiv - Genomics 2019Quote: All 192 bp enhancers were amplified from the array using HSSF-ATGC and HSS-R-clon (Supplemental Table 8) with KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems) with SYBR Green (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Illumina compatible adapters (IDT, Inc) were ligated to the mate pair fragments and amplified with 8-12 cycles of PCR (KAPA Biosystems).
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bioRxiv - Neuroscience 2020Quote: Brain tissues were homogenized in Tris-buffer (50 mM Tris pH 8, 0.25 M sucrose, EDTA-free cOmplete protease inhibitor cocktail (Roche Diagnostics 05056489001)) and centrifuged at 3600 rpm and 4°C for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... cell pellet was resuspended in prechilled buffer A (20 mM Tris/HCl, pH: 8) supplemented with cOmplete™ protease inhibitor (Roche) and 5 mM EDTA ...
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bioRxiv - Cell Biology 2022Quote: ... The lyophilized oligo pool was resuspended in Tris-HCl (pH 8) and PCR-amplified using KAPA HiFi HotStart DNA Polymerase (Roche, KK2502) with 52 °C anneal and 15 sec extension (10 cycles) ...
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bioRxiv - Neuroscience 2023Quote: ... Expi293F cell pellets (from 50 ml of culture) were thawed and resuspended in 8 ml of 0.25 x PBS with protease inhibitors (Roche, Indianapolis, USA). Cells were lysed using a Dounce homogenizer on ice and then centrifuged in a Beckman SW55 rotor at 100,000xg for 45 min ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in lysis buffer (8 M urea, 50 mM EPPS pH 8.5, 150 mM NaCl, Roche protease inhibitor tablet) and lysed by bead beating ...
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bioRxiv - Genetics 2023Quote: Cell migration assays were performed with the xCELLigence Biosensor System using specifically designed 16-well plates equipped with membranes having 8-μm pores (CIM-plate 16; Roche Diagnostics). Cells in serum-free medium were seeded in the upper chambers ...
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bioRxiv - Immunology 2023Quote: ... 1% NP-40, 150 mM NaCl, and 10 mM Tris-HCl [pH 8] supplemented with phosphatase and protease inhibitor cocktail tablets [Roche Diagnostics]). The cell lysates were cleared by centrifugation for 15 min at 40C ...
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bioRxiv - Biochemistry 2023Quote: ... resuspended in lysis buffer (50 mM NaH2PO4; 300 mM NaCl; 10 mM imidazole; pH 8: supplemented with protease inhibitor cocktail (cOmplete, Roche, Switzerland), DNaseI (ITW REAGENTS ...
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bioRxiv - Cell Biology 2023Quote: Between 20,000 and 40,000 cells were seeded the day before the imaging experiment on 8-well ibidi dishes coated with 25 μg/ml laminin (Roche, 11243217001). When indicated ...
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bioRxiv - Microbiology 2020Quote: ... Cells were seeded in 10 cm tissue-culture treated dishes and transfected with FuGENE 6® reagent (Roche) for 24 hr ...
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bioRxiv - Neuroscience 2022Quote: ... hDAT and Stx1 constructs were transiently cotransfected into these cells (hDAT cells) using Fugene-6 (Roche Molecular Biochemicals) per the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... The mini-genes in the pSPL3b vector were transiently transfected using 6µl of FuGENE 6 Transfection Reagent (Roche) with 2 µg of vector ...
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bioRxiv - Evolutionary Biology 2020Quote: ... five pupae were homogenized in 100uL of homogenization buffer (125 mM Tris pH 6.8, 6% SDS, 2.5X Roche cOmplete protease inhibitor cocktail ...
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bioRxiv - Neuroscience 2023Quote: ... Vectors were co-transfected with packaging-defective helper plasmids into 293T cells using Fugene 6 transfection reagent (Roche). Fibroblasts were plated at a density of 50,000 cells/well on 0.1% gelatin-coated 6-well plates and infected three times with a viral cocktail containing vectors expressing OCT4:SOX2:KLF4:cMYC in a 2:1:1:1 ratio in the presence of 6 µg/ml protamine sulfate (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant containing chromatin fragments was then treated with 6-10 µg/g cells DNase-free RNase (Roche) at 37°C for 30 minutes and subsequently cleared by centrifugation at 30,000 rcf for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Both were fragmented at 94°C for 6 min and ligated with KAPA Unique Dual-Indexed adaptors (Roche). Library quality was checked on an AATI (now Agilent ...