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Citations for Roche :
1701 - 1750 of 7965 citations for 5 Chloro 1 benzothiophen 2 yl methanol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Total RNA was extracted from 5-8 pairs of ovaries using an RNA extraction kit (Roche). 50 ng of total RNA was used to make cDNA using the Transcriptor First Strand cDNA Synthesis kit (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... and filtered prior to loading onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
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bioRxiv - Microbiology 2023Quote: ... resuspendend in 10 ml of PBS buffer with 5 μg/ml DNaseI (from bovine pancreas, Roche) and 10 μg/ml lysostaphin and incubated for 20 min at 37°C to allow cell lysis ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.2 μl (5 U/μl) FastStart™ Taq DNA Polymerase (Roche Diagnostics GmbH, Mannheim, Germany) in a total volume of 25 μl ...
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bioRxiv - Immunology 2023Quote: ... and equal amounts of protein from lysate were incubated with 5 μg anti-FLAG antibody (Roche) overnight ...
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bioRxiv - Biochemistry 2022Quote: ... and 63 mM adenosine 5’-triphosphate disodium salt trihydrate brought to pH 7.0 (Roche: ATPD-RO), were stored at −20°C.
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bioRxiv - Cell Biology 2023Quote: ... Each RT-qPCR reaction (10 μl) contained 5 μl of SYBR Green Select Master Mix (Roche), 300 nM of the corresponding forward and reverse primers ...
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bioRxiv - Genomics 2023Quote: ... final extension 5 min at 62 °C) using the KAPA HiFi HotStart Ready Mix (Kapa Biosystems). Amplified libraries were purified ...
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bioRxiv - Immunology 2023Quote: ... ‘reverse’ 5’-GGGTACTTGATTTCATAGACTTTA-3’) were used in a qRT-PCR on a LightCycler 480 II (Roche). The data were analysed with LightCycler® 96 SW 1.1 (Roche) ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM EDTA pH 8.0) with 1X complete protease inhibitors (cOmplete Protease Inhibitor Cocktail, Roche 11697498001) by vortexing ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µl of purified DNA is processed using the KAPA HyperPlus kit (Roche Sequencing, CA, USA) according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... 0.3% Tween-20 pH 7.5) for 5 min at 37°C and blocking solution (#11585762001, Roche) for 45 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... tissue sections were thawed at room temperature for 30 min and blocked with 5% BSA (Roche) + 0.2% Tween 20 (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... 10 µl qPCR reactions were used for qPCR: 5 µl SYBR Green I Master Mix (Roche), 0.5 µl of 10 µM forward and reverse primer (final concentration 0.5 µM each primer) ...
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bioRxiv - Biophysics 2024Quote: ... and 5 mM ATP) containing an ATP regeneration system [0.1 mg/mL pyruvate kinase (10109045001, ROCHE) and 2.5 mM phosphoenolpyruvate (860077 ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM PMSF) supplemented with 1x protease inhibitor cocktail (Roche cOmplete Protease Inhibitor Cocktail) and lysed using a Dounce homogenizer (20 strokes with loose plunger followed by 20 strokes tight plunger) ...
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bioRxiv - Cell Biology 2020Quote: ... the bile duct was cannulated and perfused with 2◻mL collagenase P (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2021Quote: ... Briefly, cells were lysed in lysis buffer (2% SDS, 1X protease inhibitor cocktail (Roche), and 1 mM sodium azide in 1X TBS ...
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bioRxiv - Neuroscience 2020Quote: ... followed by reduction and alkylation in 10 mM Tris (2-carboxyethyl) phosphine hydrochloride (Roche) and 55 mM iodo-acetamide (Sigma-Aldrich) ...
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bioRxiv - Genomics 2020Quote: cDNA products were amplified with KAPA HiFi HotStart Uracil 2 x ReadyMix (Kapa Biosystems) and newly designed PCR primers that contain a deoxyuraciland one of which was biotinylated ...
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bioRxiv - Neuroscience 2020Quote: ... pH 9.5) was applied for 2 minutes before adding CDP-star (25 mM, Roche) for 5 minutes in the dark ...
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bioRxiv - Biophysics 2021Quote: ... which was supplemented with 2 x cOmplete™ EDTA-free protease inhibitor tablets (Roche), 15 μg/mL DNase I (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA) with protease inhibitor cocktail (04693116001, Roche Life Science, Indianapolis, IN, USA) and Phosphatase Inhibitor cocktail (P0044 ...
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bioRxiv - Immunology 2020Quote: ... Livers (minced) and pooled deciduae were incubated in 2 mg/mL collagenase D (Roche) at 37 °C for 30 – 40 minutes with agitation ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 mM Tris(2-carboxyethyl) phosphine (TCEP) supplemented with a protease inhibitor cocktail (Roche,cOmplete Ultra EDTA-free ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were isolated by sequential digestion with pronase (2 mg/mL, PRON-RO, Roche) at 37 degrees ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole; 10236276001, Roche, Basel, Switzerland) for 5 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... and 2 mM NaF supplemented with complete mini protease inhibitor cocktail (Roche, Laval, QC)) ...
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bioRxiv - Systems Biology 2022Quote: ... 2% SDS) and in presence of 1x complete protease inhibitor cocktail (Roche, Basel, Switzerland). Samples were then sonicated using Bioruptor Pico (Diagenode ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were washed 2 times with 1x PBS with protease inhibitor cocktail (Roche, 11836170001). They were lysed on the plate with cold Farnham lysis buffer to ~10×10^6 cells /mL (5mM PIPES pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 infection was confirmed by the RT-PCR-COBAS 6800 System (Roche Molecular Systems ...
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bioRxiv - Physiology 2020Quote: ... The pellet was then suspended in DMEM containing 2 mg/mL collagenase/dispase (Roche), 0,147 μg/mL TLCK (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... 2 mM EDTA and cOmplete ™ EDTA-free Protease Inhibitor Cocktail (Roche product 11836170001) for 30 min on ice ...
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bioRxiv - Biophysics 2020Quote: ... containing 0.5% (w/v) SDS and 2 μg of proteinase K (Roche, cat. #3115887). DNA fragments were extracted with phenol/chloroform and analyzed by electrophoresis in a non-denaturing 10% polyacrylamide gel ...
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bioRxiv - Microbiology 2020Quote: ... 10μL of 2× FastStart TaqMan® Probe Master Mix (Roche Life Science, Mannheim, Germany), 750 nM cruzi1 and cruzi2 primers and 250 nM cruzi3 probe (FAM/NFQ-MGB ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 mM β-mercaptoethanol in the presence of EDTA-free protease inhibitor cocktail (Roche) and benzonase (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM β-mercaptoethanol in the presence of EDTA-free protease inhibitor cocktail (Roche) and benzonase (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2020Quote: ... The LD-PCR mastermix contained 6.25 μl 2× KAPA HiFi HotStart ReadyMix (Roche Diagnostics), 0.125 μl 20 μM PCR_Satija forward primer(30) ...
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bioRxiv - Genomics 2022Quote: ... PCR was performed by adding 10μl 2×HiFi PCR mix (Kapa Biosystems, Cat. 7958927001) and 0.5μl 60mM SINGV6 primer and running the following program ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and blocked for 60 minutes in PBS-T with 2% blocking reagent (Roche, UK), 5% foetal calf serum (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... 2 μl of 10 mM dNTPs and 10 units of Transcriptor Reverse Transcriptase (Roche).
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM EDTA pH 8.0 and 0.1% BSA supplemented with protease inhibitor cocktail (Roche)) ...
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bioRxiv - Neuroscience 2022Quote: ... Primers were designed using the Universal ProbeLibrary Assay Design Center (Roche, Supplementary Table 2) and transcript levels of candidate genes were measured by qRT-PCR using the TaqMan hPSC Scorecard™ Panel (384 well ...
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bioRxiv - Microbiology 2023Quote: ... DENV-2 RNA was amplified by qRT-PCR (LightCycler Multiplex RNA Virus Master, Roche), using primers to the conserved 3’UTR ...
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bioRxiv - Biochemistry 2024Quote: ... Next, 50 µl of lytic buffer was added (2% NP-40, protease inhibitors (Roche), 0.2 µM LargeBiT (produced in house ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... qPCR was performed using SYBR FAST ABI Prism 2× qPCR Master Mix (Kapa BioSystems). Amplification was carried out in a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were sequentially enzymatically treated with 2 mg/ml of Pronase (Roche 165921) in 50 mM of MES (Sigma-M8250 ...
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bioRxiv - Plant Biology 2023Quote: ... with a KAPA SYBR FAST qPCR Master Mix (2×) Kit (Kapa Biosystems, Wilmington, MA). Relative quantities were determined by the 2(-delta delta Ct ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM MgCl) with with cOmplete Protease Inhibitors Cocktail (PI, Roche Diagnostics, Rotkreuz, Switzerland) and homogenized at 4 °C using a pre-cooled Potter-Elvehjem PTFE pestle at 100 RPM and glass tube with a working volume of 30 mL ...
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bioRxiv - Cell Biology 2023Quote: ... After physical disaggregation and digestion with 2 mg/ml collagenase B (Roche, CA, USA), the enzymatic activity was stopped by diluting with PBS 1X ...