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Citations for Roche :
101 - 150 of 5652 citations for Human Mesoderm Specific Transcript Homolog Protein MEST ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the non-specific competitor poly-dI-dC (Roche) was added to the EMSA reactions at a final concentration of 2.5 ng/µL ...
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bioRxiv - Cancer Biology 2024Quote: ... and specific primers on the LightCycler 96 (Roche). Relative target gene expression was determined by comparing average threshold cycles (CT ...
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bioRxiv - Systems Biology 2020Quote: ... strand-specific RNA libraries were prepared using a KAPA Stranded RNA-Seq Library Preparation Kit (KAPA Biosystems, catalog no. KK8401) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2019Quote: ... Then one hundred nanograms of RNA per sample were used to prepare dual-indexed strand-specific cDNA libraries using the KAPA mRNA HyperPrep kit (Roche). The resulting libraries were assessed for quantity and size distribution using Qubit and Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using a Bio-Rad sequence detection system according to the manufacturer’s instructions using a double-stranded DNA-specific SYBR Green Premix Ex TaqTM II Kit (Roche, Switzerland). Experiments were conducted in duplicate in three independent assays ...
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bioRxiv - Cancer Biology 2022Quote: The activity of telomerase in the cell lines was detected using the TeloTAGGG telomerase PCR ELISA Kit (Roche, Cat# 12013789001). The assay was performed according to the manufacturer’s instructions and repeated in triplicate ...
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bioRxiv - Molecular Biology 2019Quote: ... and were then stained with the mouse monoclonal antibody against the human P16 protein (Ventana Roche-E6H4, USA) and the FITC-tagged secondary antibody ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitation of all gene transcripts was made by qPCR using a Power SYBR Green PCR Master Mix (Roche) and Thermo Quant Studio 5 sequence detection system (Thermo ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitation of all gene transcripts was performed by qPCR using a Power SYBR Green PCR Master Mix (Roche) and an ABI PRISM 7500 sequence detection system (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... Relative transcript levels were quantified by quantitative real-time PCR in 96-well plates with LightCycler 480 (Roche). The reaction was set by using qPCRBIO Probe Mix Hi-ROX (Nippongenetics ...
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bioRxiv - Microbiology 2024Quote: ... The lcrF transcripts of interest were detected with a DIG-labeled PCR fragment (DIG-PCR nucleotide mix, Roche) amplified with primer pairs for the individually detected according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... The expression of transcripts of the target gene was measured by using a LightCycle® 96 instrument (Roche) with Hieff UNICON SYBR green master mix (11198ES08 ...
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bioRxiv - Cancer Biology 2021Quote: ... RT-PCR was performed using first strand synthesis with specific primers (Reagents) and SYBR FAST qPCR Kit (Kapa Biosystems, Wilmington, MA) in a StepOne cycler (Applied Biosystems ...
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bioRxiv - Developmental Biology 2020Quote: ... Strand-specific polyA-enriched libraries were prepared using the Kapa mRNA-seq Hyper kit (Kapa Biosystems, Cape Town, South Africa KK8581). Untreated samples were sequenced on an Illumina HiSeq4000 with 1×100bp reads ...
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bioRxiv - Genomics 2021Quote: ... RNA sequencing libraries were prepared on a Beckman Coulter Biomek i7 liquid handling platform using Roche Kapa mRNA HyperPrep strand specific sample preparation kits (Roche 08098123702) from 200 ng of purified total RNA according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... RNA sequencing libraries were prepared on a Beckman Coulter Biomek i7 liquid handling platform using Roche Kapa mRNA HyperPrep strand specific sample preparation kits (Roche 08098123702) from 200 ng of purified total RNA according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... primers and probe specific for SARS-CoV-2 RdRP (TibMolBiol; #53-0777-96) and a LightCycler Multiplex RNA Virus master kit (Roche; #07083173001) according to the instructions of the manufacturer ...
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bioRxiv - Systems Biology 2023Quote: ... RNA sequencing libraries were prepared on a Beckman Coulter Biomek i7 liquid handling platform using Roche Kapa mRNA HyperPrep strand specific sample preparation kits (Roche 08098123702) from 200 ng of purified total RNA according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The V4–V5 regions of 16S rRNA gene were amplified using specific primers and libraries were constructed using the Hyper Library Preparation Kit from Kapa Biosystems (Roche Diagnostics ...
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bioRxiv - Genetics 2020Quote: ... and hybridized to human exome probes using the SeqCap EZ Prime Exome kit (Roche). The resulting exome libraries were sequenced with paired-end 150 bp Illumina reads on the HiSeq or NextSeq platforms at Admera Health (South Plainfield ...
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bioRxiv - Cancer Biology 2020Quote: Cell proliferation was measured by determining the extent of 5-Bromo-2’-deoxy-uridine (BrdU) incorporation into DNA of U87-MG cells using the BrdU cell proliferation assay ELISA kit (Roche, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Wells were pre-coated with 100 μL per well of capture anti-histone antibody contained in the Cell Death Detection ELISA kit (1:40 in 1x coating buffer; Roche 11544675001) or anti-myeloperoxidase (MPO ...
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bioRxiv - Biophysics 2020Quote: ... using gene-specific primers and KAPA SYBR (KAPA Biosystems), on a Bio-Rad CFX96 real-time PCR machine ...
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bioRxiv - Molecular Biology 2019Quote: ... Apoptosis was measured by Cell Death Detection ELISA (Roche). For reporter assays ...
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bioRxiv - Cell Biology 2019Quote: ... Relative quantification of mRNA transcripts was calculated based on an objective method using the second derivative maximum algorithm (Roche). All target genes were normalized using a stable reference gene (POLR2A) ...
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Gonads or body? Differences in gonadal and somatic photoperiodic growth response in two vole speciesbioRxiv - Neuroscience 2020Quote: ... Transcript levels were quantified by Real-Time qPCR using SYBR Green (KAPA SYBR FAST qPCR Master Mix, Kapa Biosystems). 20 μL (2 μL cDNA + 18 μL Mastermix ...
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bioRxiv - Neuroscience 2021Quote: ... Transcript levels were quantified by real-time qPCR using SYBR Green (KAPA SYBR FAST qPCR Master Mix, Kapa Biosystems). 20 μl reactions were carried out in duplo for each sample by using 96-well plates in a Fast Real-Time PCR System (StepOnePlus ...
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bioRxiv - Immunology 2023Quote: ... The transcripts for genes of interest were measured by real-time qPCR with a Lightcycler 480 II system (Roche) and a Brilliant III SYBR Green Master mix (Agilent ...
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bioRxiv - Molecular Biology 2022Quote: ... negative and positive samples contained an equivalent volume of HBsAg-specific diluent ([HBSAGQ2 Dil HepB] from the Elecsys HBsAg II quant II immunoassay kit (Roche Diagnostics International) and were used to exclude unknown contamination within the experimental setup and laboratory environment ...
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bioRxiv - Microbiology 2022Quote: ... by the capillary method and hybridised with digoxigenin-labelled blaOXA- 58 and blaNDM-1-specific probes with an NBT/BCIP colour detection kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Blots were hybridized with a digoxygenin (DIG)-labeled ZmPEPC promoter-specific probe synthesized using primers (Fwd 5’-TCCCGAGTTCCTAACCACAG; and Rev 5’-GTGGCTGAGGCTTCTTTTTG) and the PCR DIG Probe Synthesis Kit (Roche Diagnostics, Switzerland). The signals were detected by CDP-Star (Roche Diagnostics ...
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bioRxiv - Genetics 2021Quote: ... 500 ng total RNA was used for generating strand-specific RNA-seq libraries using the KAPA Stranded RNA-Seq Kit with RiboErase (Kapa Biosystems, MA). RNA-seq libraries were sequenced on an Illumina NextSeq 500 platform with paired-end 75 bp read length.
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bioRxiv - Cancer Biology 2022Quote: ... Real-time quantitative PCR was performed using gene-specific qPCR primers listed in Supplementary Materials and the KAPA SYBR Fast qPCR Master Mix (2X) kit (Kapa Biosystems, #KK4602), and ran on CFX96 Real-Time PCR Detection System (BIO-RAD ...
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bioRxiv - Neuroscience 2021Quote: ... was selected based on the levels of CAT expression which were determined in brain tissue homogenates of two-month old CAG-CAT-Prnp mice using the CAT ELISA kit (Roche, Basel, Switzerland) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The proliferative activities of VSMCs were quantified by BrdU incorporation using an enzyme-linked immunosorbent assay (ELISA) detecting kit (Roche, Mannheim, Germany) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were treated with dilutions of SB939 for 24 or 48 hours and cell proliferation was assessed using ELISA BrdU kit (Roche Diagnostics GmbH), according to manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... Gene-specific primers were designed using Universal Probe Library (Roche) and real-time PCR performed using GoTaq qPCR Master mix (Promega ...
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bioRxiv - Immunology 2020Quote: ... and HCV specific PCR primers in LightCycler 480 instrument (Roche). By absolute quantification method ...
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bioRxiv - Developmental Biology 2021Quote: ... brn3a1 and brn3a2(l) transcripts were visualized with anti digoxigenin-alkaline phosphatase conjugates and BCIP/NBT or BM purple substrates (Roche). For two color WISH krx-20 (Oxytoby and Jowett ...
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bioRxiv - Genomics 2019Quote: ... Five to six samples at a time were then multiplexed and underwent enrichment for a 43-gene targeted RNA fusion panel (Table 1) using Roche SeqCap RNA Choice target enrichment probes spanning the entirety of the gene transcripts of interest (Roche Sequencing ...
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bioRxiv - Immunology 2022Quote: RU5 and Gag reverse transcripts and integrated HIV-DNA levels were quantified by nested real-time PCR (Light Cycler 480, Roche). Experiments were performed in triplicates on 0.5-1×105 T-cells/PCR ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was reverse-transcribed into cDNA and quantification of transcripts was performed as described previously (Duy et al., 2007) using a LightCycler system (Roche). All signals were normalized to that of actin 2 (At3g18780) ...
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bioRxiv - Molecular Biology 2019Quote: ... and CAT expression was quantified using the CAT ELISA (Roche) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... and Cell Death ELISA (Roche, 11774425001, plasma dilution 1:2) which estimates cytoplasmic histone-associated DNA fragments (mono-and oligonucleosomes ...
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bioRxiv - Biochemistry 2022Quote: ... Blocking Reagent for ELISA (BRE) (11112589001) was manufactured by Roche. 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC ...
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bioRxiv - Biochemistry 2022Quote: ... Blocking reagent for ELISA (BRE, cat. 11112589001) was from Roche. DC Assay kit was from Bio-Rad ...
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Dissemination of linezolid-resistance through sex pheromone plasmid transfer in Enterococcs faecalisbioRxiv - Microbiology 2019Quote: ... blots were hybridized with digoxigenin (DIG)-labeled optrA-specific probe using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche Applied Sciences, Germany) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... was subjected to second-strand synthesis in a 25 μl reaction volume using IGHV gene–specific primers (primer No.9–14) and a KAPA Biosystems kit (Roche, KAPA HiFi HotStart). The PCR conditions were as follows ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and total serum protein (TSP) were determined using kits supplied by Roche Diagnostics and the Roche/Hitachi Analyzer machine at Al-Aulaqi Specialized Medical Laboratory ...
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bioRxiv - Bioengineering 2019Quote: ... Human Fibronectin (Roche). Agar low melting point ...