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Citations for Roche :
301 - 350 of 5652 citations for Human Mesoderm Specific Transcript Homolog Protein MEST ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The F9 teratocarcinoma cells were grown in DMEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The F9 teratocarcinoma cells were grown in DMEM (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... and 100 U/mL human interleukin 2 (IL-2) (Roche) (Munoz et al. ...
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bioRxiv - Biophysics 2019Quote: ... and 30 IU/mL human interleukin-2 (hIL-2) (Roche).
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bioRxiv - Cell Biology 2021Quote: ... human NEMO (5 µg) and ATP (2 mM) (Roche, 10519979000) were incubated at 37°C for the indicated time in a buffer containing 150 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... coated with 5 µg/ml human plasma fibronectin (Roche, 11080938001) in growth medium overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... and human Ki67 rabbit monoclonal antibody (Roche Diagnostics, 790-4286). Images were captured with VENTANA iScan HT (Roche Diagnostics ...
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bioRxiv - Immunology 2023Quote: ... 100 IU/ml of recombinant human IL-2 (Roche Diagnostics) or 10 μg/ml of SIVmac251 Env gp130 (ImmunoDx) ...
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bioRxiv - Immunology 2023Quote: ... and 30 IU/mL human interleukin-2 (hIL-2) (Roche). To ectopically express Lifeact-eGFP or Lamp1-eGFP in CTLs ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.01 mg/mL of human insulin (Roche, Indianapolis, IN, USA), 10 mM HEPES (Corning ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCR was performed with gene specific p rimers (see Key Resource Table) using Sybr green detection on a Light Cycler 480 (Roche) or Quantstudio6 (Applied Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... 5 pmol of the forward and reverse gene-specific primers each in Light Cycler SYBR Green I Master mix (Roche) on LightCycler 480 II (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was performed with the specific primers listed in Table 2 using the LightCycler 480 (Roche, Manheim, Germany).
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bioRxiv - Developmental Biology 2022Quote: The different CRE candidate sequences were amplified using PCR-specific primers (see Table S2) and the Expand High Fidelity PCR system (Roche). Amplified fragments were gel-purified and cloned into the pSK-lacZ vector as in (Beccari et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... Sublibrary oligo pools were amplified individually using window-specific primer pairs and KAPA HiFi HotStart ReadyMix (Roche, Indianapolis, IN, USA) with a final template concentration of 1:2000 resuspended oligo array and annealing temperatures of either 60 °C or 65 °C (depending on performance of individual primer pairs ...
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bioRxiv - Microbiology 2020Quote: ... was used for each time point (mid-log and stationary) along with gene-specific primer and SYBR Green master mix (Roche) in a 10 μL reaction according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA was used as template for RT-PCR with an adapter-specific primer (BRevOligo) and 10 units of Transcriptor Reverse Transcriptase (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... 5 pmol of the forward and reverse gene-specific primers each (primer sequences are shown in Table S3) in Light Cycler SYBR Green I Master mix (Roche) on LightCycler 480 II (Roche) ...
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bioRxiv - Systems Biology 2019Quote: ... qPCR was performed using the Taqman or SYBR green system with sequence-specific primers and/or the Universal Probe Library (Roche). All data were analyzed with 18S or β-actin as the endogenous control ...
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bioRxiv - Evolutionary Biology 2021Quote: ... were synthesized from cDNA templates via T3 RNA polymerase mediated strand-specific RNA transcription using DIG/FL RNA labelling mix (Roche). Hybridised ...
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bioRxiv - Neuroscience 2022Quote: ... 1 µg of Capture-C library per sample was mixed with mouse COT1 DNA and universal as well as index-specific blocking oligos from SeqCap EZ HE-oligo (Roche). 4.5 µl pool of biotinylated probes (xGen Lockdown Probe Pools from IDT) ...
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bioRxiv - Cancer Biology 2022Quote: ... qRT-PCR was done in triplicate with PerfeCTa qPCR SuperMix Low ROX (Quanta Biosciences) with gene specific probes (Universal Probe Library, UPL, Roche) and primers ...
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bioRxiv - Neuroscience 2022Quote: ... high specific-activity RNA probes were produced from a linearized plasmid (HindIII) and labeled using the Fluorescein RNA Labeling Mix (Roche), following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... was designed to tile a 250 bp capture region within the central 4-kb target region from each 10-kb window of hg19 avoiding non-specific sequences by Roche so that the maximum distance between 2 capture regions is 14-kb ...
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bioRxiv - Developmental Biology 2020Quote: ... 5 pmol of the forward and reverse gene-specific primers each (Supplemental Table 3) in Light Cycler SYBR Green I Master mix (Roche) on LightCycler 480 II (Roche) ...
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bioRxiv - Immunology 2020Quote: ... Cells were cultured for 24 hours in the presence of SARS-CoV-2 specific MPs [1 μg/mL] or 5 μg/mL phytohemagglutinin (PHA, Roche) in 96-wells U-bottom plates at 1×106 PBMCs per well ...
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bioRxiv - Developmental Biology 2020Quote: ... 5 pmol of the forward and reverse gene-specific primers each (primer sequences shown in table S2) in Light Cycler SYBR Green I Master mix (Roche) on LightCycler 480 II (Roche) ...
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bioRxiv - Genomics 2020Quote: ... Quantitative PCR reactions were prepared using the FastStart DNA Essential DNA Green Master and sequence-specific primers (Supplementary Table S3 and analyzed using a Roche Light Cycler®96 ...
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bioRxiv - Immunology 2021Quote: ... 2.5µL diluted cDNA was used as template in a 10µL qRT-PCR reaction performed in duplicate using gene-specific primers and Kapa SYBR Green Universal qPCR mix (KAPA Biosystems) according to manufacturer’s instructions using a BioRad CFX Connect Real-Time PCR System ...
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bioRxiv - Cancer Biology 2019Quote: Real-time PCR analysis of cDNA samples was performed with specific primers and probes designed by using Assay Design Center (Roche). Primers and probes used here are available upon request ...
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bioRxiv - Genomics 2021Quote: ... 1μg of Capture-C library per sample was mixed with mouse COT1 DNA and universal as well as index-specific blocking oligos from SeqCap EZ HE-oligo (Roche). 4.5μl pool of biotinylated probes (xGen Lockdown Probe Pools from IDT) ...
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bioRxiv - Immunology 2020Quote: ... Knockdown of A3A is confirmed by RT-qPCR using allele-specific primers and SDHB probe on a Light Cycler 480 System (Roche), as previously described (Baysal et al ...
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bioRxiv - Cell Biology 2020Quote: ... reverse transcribed with qScript cDNA SuperMix (Quanta Biosciences) and primer-specific amplified with the quantitative PCR MasterMix FastStart Universal SYBR Green (Roche) or the TaqMan® Universal Master Mix II when using the Universal Probe Library (Roche) ...
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TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thalianabioRxiv - Plant Biology 2022Quote: ... was performed using gene specific primers (see File S2) in a total volume of 10 μl SYBR Green Master mix (Roche) on a LightCycler LC480 apparatus (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... The 20μL reverse transcription product was diluted with 60μL of Invitrogen nuclease free water and analyzed using sequence-specific qPCR primers and probes (Integrated DNA Technologies) and Light Cycler 480 Probes Master Mix (Roche) for relative quantitation of RNA on a CFX96 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Genomics 2023Quote: ... and incorporation of sample-specific multiplex indices before PCR amplification (12 cycles) according to the manufacturer’s protocol (Kapa Biosystems, USA). Size distribution and molarity of amplified cDNA libraries were assessed via the Bioanalyzer High Sensitivity DNA Assay (Agilent Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... Reactions of qRT-PCR were set up with 50-70ng of cDNA and 250 nM gene-specific primers using FastStart Universal Sybr Green Master (Rox) mix (Roche). Fold change in transcript levels for deregulated genes was calculated as follows ...
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bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a, 1:10; CD20, Ventana Roche L26 ...
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bioRxiv - Genomics 2022Quote: ... Re-purified circular fragments were amplified by PCR with View-Point specific primers (see Supplementary Table 2) using the Expand Long Template PCR System (Roche). Resulting amplicons were purified with a 0.8× ratio of AMPure XP beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: ... was used for cDNA synthesis and qRT-PCR was performed using FastStart Essential DNA Probes Master and gene specific probes from Universal Probe Library (Roche). Lightcycler 96 (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... Ribosomal RNA was removed by enzymatic digestion of the specific probe-bound duplex rRNA (KAPA RNA Hyper+RiboErase HMR, Roche) and then fragmented to around 200-300bp with heat in the fragmentation buffer ...
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bioRxiv - Neuroscience 2024Quote: ... 20 ng of DNA was analyzed by qPCR using primers specific for vector DNA (GFP) and genomic DNA (ADCK3) on a LightCycler 480 II (Roche). Viral genomes per cell were calculated by dividing total viral genomes (detected by GFP ...
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bioRxiv - Cell Biology 2019Quote: ... and levels of p24 were determined by ELISA as previously described (65) The SEAP levels of the supernatants were determined by a chemiluminescent method (Roche) and were used to normalize the differences in transfection efficiencies.
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bioRxiv - Biochemistry 2020Quote: RT activity was measured using an ELISA-based colorimetric reverse transcriptase activity assay (Catalog No. 11468120910, Roche Diagnostics, Indianapolis, IN). The HEPES integration buffer was used for the reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 µl protein A/G beads (SantaCruz) in the presence of Complete Protein Inhibitor (Roche) and 40 U RNase inhibitors (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins and input proteins were analyzed by immunoblotting with α-GFP (Roche, Cat # 11814460001) or α-BAK1 antibodies 55.
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bioRxiv - Bioengineering 2022Quote: ... S195A-GD-FXa protein was further purified on an anti-Protein C Affinity matrix (Roche). Maldi mass spectrometry showed that TFPI was glycosylated ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated human MIF was produced using D-biotinoyl- ε -aminocaproic acid-N-hydroxy-succinimide ester (Biotin-7-NHS) with the Biotin Protein Labeling Kit from Roche (Mannheim, Germany). Alternatively ...
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bioRxiv - Developmental Biology 2022Quote: Testes from adult mice were cut into ~20 mg pieces and transferred into ~200 μl pre-cooled denaturing buffer from the Minute Total Protein Extraction Kit (SD-001, Invent Biotech, Plymouth, MN, USA) containing protease inhibitors (CO-RO, Roche, Indianapolis, IN, USA) and phosphatase inhibitors (PHOSS-RO ...
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bioRxiv - Cancer Biology 2020Quote: ... Il1r1/IL1R1 and Gapdh/GAPDH qPCR was performed using specific primers and Lightcycler 480 Sybr Green I Master (Roche; Mannheim, Germany) in a Lightcycler 480 II (Roche Diagnostics) ...