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Citations for Roche :
1401 - 1450 of 6347 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Quantitative RT-PCR (qRT-PCR) reaction was performed using cDNA with SYBR green fast mix (Quantabio, PerfeCTa, 95072-012) on a Roche lightcycler (Roche, LightCycler 480 II). The following primers used for qRT-PCR ...
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bioRxiv - Immunology 2021Quote: ... Ct values for Asns and the housekeeping gene Actb (Beta-actin) were determined by quantitative real-time PCR on a LightCycler® 480 II Real-Time PCR System (Roche Life Science), using the TB Green™ Premix Ex Taq™ (Takara ...
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bioRxiv - Microbiology 2021Quote: 16S rRNA gene concentrations in purified genomic DNA extracts were quantified using SYBR Green I Master on a LightCycler 480 II (Roche Molecular Systems, Inc.). The primer pairs for Bacteria and Archaea were Bac908F_mod (5’ s-AAC TCA AAK GAA TTG ACG GG-3′ ...
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bioRxiv - Cell Biology 2022Quote: ... Quantification of the mRNAs of interest was carried out on a Roche Lightcycler™ 480 II instrument by using SYBR-Green I Master mix (Roche, Basel, Switzerland) and primers specified in the Table bellow ...
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bioRxiv - Microbiology 2021Quote: ... tissue pieces were incubated with their epidermal side facing up in RPMI 1640 medium supplemented with 2.4 U/ml Dispase II (Roche Diagnostics GmbH, Mannheim, Germany) overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The qRT-PCR was performed with the Hieff® qPCR SYBR® Green Master Mix (Yeasen, China) and a LightCycler 480 II Instrument (Roche, Switzerland). Six biological replicates and three reaction replicates for each group were used ...
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bioRxiv - Plant Biology 2019Quote: ... were used as template for quantitative real time RT-PCRs which were performed in 384-well plates using a LightCycler 480 II detection system (Roche Diagnostics, Mannheim, Germany). GoTaq PCR Mastermix (Promega ...
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bioRxiv - Bioengineering 2019Quote: ... The qPCR assay was performed on a Roche LightCycler® 480 Instrument II using LightCycler® 480 Probes Master (Roche Applied Science, Germany), UniWDVfw and UniWDVrv primers [33] and a TaqMan probe (6-FAM-AGGCGAAGAATGATTCACCCT-BHQ-1) ...
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bioRxiv - Genetics 2020Quote: ... Real-time PCR was performed with a LightCycler® 480 II and a LightCycler®480 SYBR Green I Master (Roche, Basel, Switzerland). The relative expression level of the CRISPR mRNA was standardized using GAPDH expression ...
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bioRxiv - Physiology 2020Quote: ... finely cut with scissors and incubated in digestion medium (PBS with 2% BSA, 1M CaCl2, 94U/ml dispase II and 100mg/ml collagenase D, Roche Life Science, France) at 37°C for 30-40min ...
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bioRxiv - Microbiology 2022Quote: ... and thiosulfohydrolase (soxB) of sulfide oxidation were quantified by SYBR-Green based qPCR assays using a LightCycler 480 II (Roche Life Science, Switzerland). Published primer pairs and a newly designed primer set for soxB were applied (Tables S1 ...
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bioRxiv - Physiology 2023Quote: ... The qPCR program was initiated at 95°C for 10 minutes to denature the cDNA and activate the TAQ polymerase enzyme in a thermocycler (Lightcycler® 480 II Roche thermocycler, Roche Diagnostics, Switzerland). The initiation was followed by 45 amplification cycles ...
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bioRxiv - Molecular Biology 2022Quote: In vitro transcribed RNA was prepared from linearized plasmid containing the human PTH 3’-UTR (68) using a Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... kidney (human primary renal proximal tubule epithelial cells, RPTEC) and liver (human hepatocellular carcinoma, HepG2) cells were assessed using a standard WST-1 (Roche) cell viability assay ...
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bioRxiv - Microbiology 2020Quote: Damage to the human colon epithelial cell line HT-29 was assessed using a lactate dehydrogenase (LDH) cytotoxicity detection kitPLUS (Roche), which measures the release of the LDH enzyme in the growth medium ...
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bioRxiv - Cell Biology 2020Quote: ... MEFs were seeded subconfluently o/n (unless indicated otherwise) onto coverslips acid-washed and coated with human fibronectin (25 μg/ml, #11051407001, Roche), as described (Dimchev and Rottner ...
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bioRxiv - Cancer Biology 2022Quote: ... All embryonic histology slides and human tissue microarray (BioMax, U.S. #BC001134b) slides were scanned using a Ventana DP200 slide scanning system (Roche Diagnostics) at 20x magnification ...
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bioRxiv - Synthetic Biology 2019Quote: ... and the candidates were PCR amplified from a U2OS (human bone osteosarcoma cell line) genome prep as template with the Kapa Hifi Hotstart Polymerase (Roche) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: A comprehensive list of coordinates of all the exonic and conserved regulatory elements from human X chromosome was used to design a customized capture library from Roche, NimbleGen (Supplementary Table 1) ...
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bioRxiv - Cell Biology 2021Quote: Human pluripotent stem cells (hPSCs) were maintained in E8 medium and passaged every 4 days onto matrigel-coated plates (Roche). The following hPSC lines were used in the study ...
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bioRxiv - Developmental Biology 2021Quote: H9 human pluripotent stem cells were maintained in E8 media and passaged every four days onto matrigel-coated plates (Roche). ESCs ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T-cells were plated in 200ul of medium (RPMI, 10% human serum) containing IL-2 (Roche, 10 IU/mL) and IL-7 (Peprotech ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... kidney (human primary renal proximal tubule epithelial cells, RPTEC), and liver (human hepatocellular carcinoma, HepG2) [45–50] cells were assessed using a standard WST-1 (Roche) cell viability assay ...
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bioRxiv - Immunology 2023Quote: K2 cells or human neutrophils were lysed with 150-200 µl of lysis buffer (supp. Table 2) supplemented with 1X complete inhibitor (Roche) and incubated for 10 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: Frozen brain tissues from human and animals were used to prepare 10% (w/v) homogenates in RIPA buffer containing PI and PhosStop (Roche). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... followed by library preparation with KAPA RNA HyperPrep kit (Roche, kit code KK8541), according to manufacturer instructions ...
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bioRxiv - Biochemistry 2021Quote: Quantitative RT-PCR was performed on the Roche LightCycler 480 Instrument II system using the LightCycler 480 SYBR Green I Master (Roche Applied Science, Mannheim, Germany). Template cDNA was diluted 1:10 ...
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bioRxiv - Cell Biology 2020Quote: ... qPCR was conducted using Roche SYBR-Green® master mix and a LightCycler 480 II Real-Time PCR system (Roche Applied Science, Penzberg, Germany). For analysis ...
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bioRxiv - Biochemistry 2020Quote: ... Primer efficiencies were evaluated by RT-qPCR using LightCycler® 480 SYBR Green I Master and LightCycler® 480 instrument II (Roche, Bâle, Switzerland). Gene expression was quantified using the Biomark microfluidic system ...
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bioRxiv - Plant Biology 2022Quote: ... using an Ovation Ultralow V2 kit (NuGEN) or a Kapa DNA HyperPrep Kit (Roche) in combination with IDT UD barcodes (Illumina) ...
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bioRxiv - Developmental Biology 2023Quote: ... Riboprobes were synthesized by DIG RNA labeling kit and in vitro transcription kit (Roche Applied science ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were quantified using the KAPA Library Quanitification Kits - Complete kit (Universal) (Kapa Biosystems). Average size fragment was determined using a LabChip GXII (PerkinElmer ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA probes were generated via overnight incubation at 37°C using 20 U/µL T7 RNA polymerase (Roche) and digoxigenin (DIG)-labeled dNTPs (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... Final DNA concentration of 1μM and increasing concentrations of Stl (0.5-8 μM) were mixed with 1μg/ml poly(d[I-C]) (Roche) in EMSA buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2019Quote: ... The membrane was incubated overnight (O/N) at 4°C with mouse anti-α-tubulin (Roche, loading control) at 1:4000 ...
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bioRxiv - Cell Biology 2019Quote: ... cardiomyocytes were dissociated using DPBS for 20 min at 37 °C followed by 1:200 Liberase TH (Roche) diluted in DPBS for 20 min at 37 °C ...
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bioRxiv - Genetics 2022Quote: ... Overnight incubation with Anti-Dig antibody conjugated to alkaline phosphatase (1:5,000) at 4 °C (no. 11093274910; Roche) was followed by 8 × 30 min washing steps at room temperature with TBST 2 (TBST with 0.1% Tween 20 and 0.05% levamisole–tetramisole ...
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bioRxiv - Genomics 2022Quote: ... Slides were washed at 42°C in 0.1xSSC and hybridization sites were detected with anti-digoxigenin-FITC (2µg/ml; Roche) and Streptavidin-Alexa594 (1µg/ml ...
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bioRxiv - Bioengineering 2022Quote: ... The dermal sections were digested for 1 hour at 37°C in 0.5 mg/ml Liberase TM (Roche) in serum free RPMI (Gibco + 2mM glutamine ...
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bioRxiv - Neuroscience 2021Quote: ... pre-cleared supernatant (25µl beads and 200µl supernatant, 30min, 4°C) was first incubated with anti-HA antibody 12CA5 (Roche Life Science ...
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bioRxiv - Immunology 2020Quote: ... The eluate was then treated with RNaseA for 1 h at 37°C and with Proteinase K (Roche) for 1 h at 55°C and the DNA was recovered using a Qiagen PCR purification kit.
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bioRxiv - Microbiology 2022Quote: ... and the right inferior lung lobes were digested at 37°C with 630 µg/mL collagenase D (Roche) and 75 U/mL of DNase I (Sigma–Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Skin samples were excised and incubated at 37°C for 90 min in dispase (2.5 mg/ml; Roche) or in liberase (0.25 mg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated overnight at 4 °C with alkaline phosphatase–conjugated anti-digoxigenin antibodies in TBTX (1:2000; Roche). Following extensive washing in TBTX ...
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bioRxiv - Cancer Biology 2021Quote: ... and digested for 30 minutes at 37 °C in DMEM + 2 mg/mL Collagenase A (Roche, Basel, Switzerland) + DNase (Roche ...
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bioRxiv - Genetics 2019Quote: ... Samples were then incubated at 55 °C for 1h after addition of 40 μg of Proteinase K (Roche) and ...
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bioRxiv - Genomics 2019Quote: ... Probes were bound to 2 µg nuclear extracts pre-incubated with 1 µg poly d(I-C) (Roche) or 100-750ng YY1 full-length recombinant protein (31332 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by a 20-min incubation at 37°C in CSS containing 1.5 mg/ml Collagenase D (Roche), 0.6 mM EDTA ...
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bioRxiv - Plant Biology 2019Quote: ... and incubated with anti-c-Myc primary antibody solution (GenScript, 1: 2,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche]) overnight at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... triglycerides and high-density lipoprotein cholesterol (HDL-c) concentrations using a Cobas C111 analyzer (Roche Diagnostics, Basilea, Sw). The intra- and inter-assay variation coefficients were less 5% in all determinations.