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Citations for Roche :
1651 - 1700 of 6347 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... Tissue slices were incubated with hybridization probes for 18 h at 55°C and then incubated with sheep anti-DIG and anti-FITC antibodies conjugated with peroxidase (POD; 1:250; Roche) for probe labeling using the InSituPro VSI robot (Intavis ...
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bioRxiv - Systems Biology 2019Quote: ... 25ml of pre-warmed to 37°C EGTA buffer followed by 25ml of pre-warmed to 37°C EBS buffer with 2.3U of Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) were cannulated into the vena cava ...
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bioRxiv - Cell Biology 2019Quote: To generate a peptide spanning lysin 105 and 106 the immunoprecipitates were digested in solution with 400 ng Glu-C (Sequencing Grade, Roche) at 37C for 16 hours.
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bioRxiv - Microbiology 2019Quote: ... The membranes were then probed for 16 h at 4 °C with the following primary antibodies in 3% (w/v) skim milk in PBS: mouse anti-GFP (1:1000, Roche), rabbit anti-SBP1 (1:1000 ...
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bioRxiv - Genomics 2019Quote: ... To remove RNA in the RNA:cDNA duplex the sample was incubated for one hour at 37° C with 125 mU/µl RNase H (Y9220F, Enzymatics) and 500 ng/µl RNase A (11579681001, Roche) in 1 × Rnase H buffer (Y9220F ...
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bioRxiv - Developmental Biology 2019Quote: ... probes were synthesized at 37°C for 2-4 hrs in digoxigenin-synthesis reaction mixture with T3 RNA polymerase (Roche). After synthesis ...
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bioRxiv - Neuroscience 2019Quote: ... The suspended pellet was incubated under agitation for 30 min at 37 °C and then digested at 56°C for 30 min with 20 μl of 50 mg/ml proteinase K (315836; Roche) in 200 μl of buffer AL ...
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bioRxiv - Microbiology 2019Quote: ... The reaction was conducted in a Versant kPCR (Siemens) at 65°C for 40 minutes for BAL samples and in a Lightcycler (Roche) at 65ºC for 30 minutes for EA/BAS ...
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Elevated Hoxb5b expands vagal neural crest pool and blocks enteric neuronal development in zebrafishbioRxiv - Developmental Biology 2021Quote: ... Probed embryos were blocked for 1-2 hours at ambient temperature in 5% Goat sera in PBST before detection overnight (∼16 hours) at 4°C using an anti-Digoxigenin-Fab fragments conjugated to Alkaline Phosphatase enzymes (1:1000 dilution, Roche) in 5% Goat Sera in PBST ...
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bioRxiv - Immunology 2021Quote: ... The reverse transcriptase was inactivated at 70 °C for 15 min and single stranded cDNA was subsequently amplified using Kapa HiFi HotStart Readymix (Roche) at a 1x concentration together with 250 nM UP-primer* (Metabion ...
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bioRxiv - Cell Biology 2020Quote: ... the membranes were hybridized overnight at 40°C with digoxigenin (DIG)-labeled DNA probes in DIG Easy Hyb solution (Roche). After low and high stringency washes ...
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bioRxiv - Bioengineering 2020Quote: ... Tissues were finely diced manually with standard razor blades and digested for 45 min at 37°C with 1.67 Wünsch U/mL (0.5 mg/mL) Liberase TL (Roche Diagnostics, Sigma Aldrich) and 0.2 mg/mL DNase I (Roche Diagnostics ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were incubated live for 10 min at 37 °C with anti-HA rat monoclonal antibody (1:100, 11867423001, Roche) or anti-GFP mouse monoclonal antibody (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... the lysates were centrifuged (10 min, 20000 g, 4 °C) and the resulting supernatants were added to 25 µl Protein G Agarose (Roche), rotating for 1 hour at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Northern blot analysis was performed at 50°C using protocol provided in the DIG Application Manual for Filter Hybridization (Roche). Following the hybridization ...
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bioRxiv - Microbiology 2020Quote: ... for 30 minutes at 37°C and 5 µg was converted to cDNA by reverse transcription using Transcriptor reverse transcriptase (Roche) and random hexaoligos (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... the sections were incubated overnight at 4 °C in digoxigenin antibodies conjugated to horseradish peroxidase (anti-digoxigenin-POD; Fab fragment; 1:100; Roche), rinsed in TBS (0.1 M Tris-HCl with 0.9% NaCl ...
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bioRxiv - Cancer Biology 2022Quote: ... The eluted chromatin was then treated with RNaseA (10mg/mL) for 1 hour at 37°C and with Proteinase K (Roche) for 2 hours at 55°C ...
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bioRxiv - Microbiology 2022Quote: PPs were excised from the SI and digested (37 °C, 45 min) using 100 µg/ml liberase TH/DNase (Roche) in RPMI containing 5 % FCS ...
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bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche] ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated overnight at +4 °C with the antibody anti-digoxigenin-AP Fab fragment (#11093274910, Roche, 1:1000) diluted in blocking solution in a humidity chamber ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were then incubated overnight at 4 °C with primary antibody of interest diluted in blocking solution (Western Blotting Reagent, Roche). The appropriate species of HRP-conjugated secondary antibody (from Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at 4°C with primary antibody for rat anti-HA (1:200; 12013819001, RRID:AB_390917, Roche, Basel, Switzerland), rabbit anti-GFP (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... This was spun down (5 minutes at 1800 RMP, 4°C) and the pellet then digested in 10ml collagenase/dispase (1mg/ml; Roche) and DNase I type IV (40µg/ml ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was immobilized using UV crosslinking (125 mJ/cm2) and hybridized at 42°C using Abion ULTRAhyb hybridization buffer and PCR DIG probes (Roche) synthesised according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: 50 million cell pellets were resuspended in 2.5ml ice cold Hi-C Lysis Buffer (10mM Tris HCl, 10mM NaCl, 0.2% NP-40, 1X protease inhibitors (Roche, 04693124001)) and split into 10 million cell amounts ...
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bioRxiv - Molecular Biology 2020Quote: ... Explants were diced and then digested by sequential incubations with pronase (70 U/mL for 1 hour at 37°C; Roche) and collagenase (0.2% w/v ...
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bioRxiv - Immunology 2020Quote: The Fab fragment of antibody CR3022 was prepared by digestion of the full-length IgG using enzyme Lys-C (Roche). The digestion reaction was allowed to proceed for 2.5 hours at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... and PI) and de-glycosylated for 3 h at 37 °C with 1 unit of PNGase-F (Roche Applied Science) added per 10 μl volume ...
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bioRxiv - Biochemistry 2021Quote: Mouse serum creatinine and urea levels were measured by the central laboratory of the University Hospital Cologne using a Cobas C 702 (Roche Diagnostics GmbH ...
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bioRxiv - Bioengineering 2021Quote: ... The skin was finely minced with a scalpel and placed for 30 min at 37 °C on a shaker in a digesting enzyme cocktail of 2 mg/ml Collagenase P (Roche), 2 mg/ml Dispase (Gibco ...
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bioRxiv - Biophysics 2021Quote: ... including 200 μM final concentration of each dNTP (G,C,T,A) and 10 µM Bio-16-dUTP or Dig-11-dUTP (all from Roche). Labelled handles specifically bind either to a glass surface covered with anti-digoxigenins or to superparamagnetic beads covered with streptavidin ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were treated for 1 hr at 55⁰C with proteinase K solution (0.4 mg/mL proteinase K (Roche; 3115828001), 50 mM EDTA ...
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bioRxiv - Genomics 2022Quote: ... The eluted material and input were incubated for 6 h at 65°C to reverse crosslinks and were treated with 100 ng RNaseA (Roche) for 1 h at 50°C ...
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bioRxiv - Cell Biology 2022Quote: ... incubation at 4 °C rotating for 3 h, followed by incubation (4 °C, rotating) with 30 μl of pre-washed Protein A agarose bead slurry (#11134515001, Roche) for an additional hour ...
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bioRxiv - Immunology 2019Quote: ... LN were pierced and torn with sharp forceps in 24-well plates and incubated for 15 min at 37° C in 1 ml digestion buffer (100 U/ml collagenase A (Roche), 500 U/ml collagenase D (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... for 1 h at 37°C and protein was removed by incubation with 10 μL of proteinase K (18 mg/mL; Roche) for 2 h at 65°C ...
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bioRxiv - Neuroscience 2019Quote: ... and then incubated overnight at 4°C with sheep anti-DIG alkaline phosphatase conjugated antibody (1:2,000; Roche Applied Sciences). Sections were washed thrice with PTW buffer and twice with coloration buffer (100 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-Green Fluorescent Protein overnight at 4°C (Clones 7.1 and 13.1; 1:50 dilution, Roche, Mannheim, Germany) and rat monoclonal anti-HA High Affinity for 60 minutes at room temperature (clone 3F10 ...
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bioRxiv - Microbiology 2019Quote: ... Protein extracts were incubated for 3 h on a spinning wheel at 4°C with 40 µl of Protein G Sepharose beads (Roche) and 2.5 µg of the specific BTV-NS3 antibody ...
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bioRxiv - Microbiology 2019Quote: ... The RNA samples were subsequently incubated for 20 min at 37 °C with 2 U of DNase I (Roche, Germany). The absence of DNA contamination in the samples was checked by the lack of conventional PCR amplification of the GP43 gene in the isolated RNA ...
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bioRxiv - Cell Biology 2019Quote: All steps were carried out on ice/at 4°C and all used buffers contained in addition Protease inhibitor cocktail tablets (cOmplete Tablets; Roche) and 0.5 mM PMSF ...
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bioRxiv - Immunology 2020Quote: ... mice were perfused with sterile PBS and the right inferior lung lobes were digested at 37°C with 630 µg/ml collagenase D (Roche) and 75 U/ml DNase I (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... They were then blocked in PBS with 3% BSA for 3h at RT and incubated overnight at 4°C with a peroxidase-labeled anti-DIG antibody (Roche) diluted 1:1500 in PBS with 3% BSA ...
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bioRxiv - Immunology 2020Quote: ... intestines were further chopped and incubated for 30 min at 37°C in medium containing liberase (20 µg/ml, Roche) and DNAse (400 µg/ml ...
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bioRxiv - Pathology 2020Quote: ... Tween-20 0.1%) for 1 hour and incubated overnight at 4 °C with the appropriated antibodies: HA (dilution 1:1000, Roche, #867423001), ß-tubulin (dilution 1:5000 ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 μL of 25 × diluted oligonucleotides and 50 ng of the digested and dephosphorylated vector were ligated overnight at 16°C using T4 Ligase (Roche), and transformed into XL10-Gold E ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% sheep serum in PBST) for one hour and overnight at 4 °C with alkaline phosphatase-conjugated anti-DIG antibodies (Roche) diluted at 1:4000 in the blocking solution ...
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bioRxiv - Genomics 2021Quote: ... Chromatin cross-linking was reversed at 65°C for 5 hours with 280 mM NaCl and 88 μg/mL RNase DNase free (11119915001, Roche). Proteins were then digested using 88 μg/mL of Proteinase K (03115828001 ...
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bioRxiv - Molecular Biology 2021Quote: Embryo extracts were pre-cleared with empty beads equilibrated in IPP 150 for 30 min at 4°C in the presence of 1x complete protease inhibitor cocktail (Roche). Pre-cleared extracts (300-500 µg ...