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Citations for Roche :
1401 - 1450 of 7954 citations for 6H Imidazo 4 5 1 de acridin 6 one 5 3 diethylamino propyl amino 8 hydroxy since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... and resuspended in Laemmli sample buffer (2% SDS, 5% β 50 mM Tris-Cl pH 6.8) supplemented with Protease Inhibitor Cocktail and PhosStop (Roche) for isolation of total cellular protein ...
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bioRxiv - Cell Biology 2019Quote: Zebrafish samples for Western blot analysis were deyolked and directly lysed in 2x Laemmli Sample Buffer containing 5% b-ME and a PhosSTOP tablet (Roche), 10 ul per embryo unless otherwise indicated ...
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bioRxiv - Cell Biology 2019Quote: ... HUVEC cells for Western blot analysis were lysed directly in 2x Laemmli Sample Buffer containing 5% b-ME and a PhosSTOP tablet (Roche), 500ul per T-25 culture flask.
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bioRxiv - Cell Biology 2019Quote: ... 5 pmol of the forward and reverse gene-specific primers each in Light Cycler SYBR Green I Master mix (Roche) on LightCycler 480 II (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... Seminiferous tubules retained on the filter were collected and incubated at 32 °C for 25 min in GBSS containing 1.2 mg/mL of Collagenase Type I and 5 μg/mL DNase (Roche 11284932). Cell aggregates were sheared gently by 10 rounds of pipetting with a wide orifice plastic transfer pipet and filtered through the Cell strainer to remove cell clumps ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was divided into 10 μl reactions containing 5 μg of RNA and was treated for 30 mins with 10 units of DNAse I (#04716728001; Roche). Then DNAse I was inactivated by addition of 1 μl of 25 mM EDTA and incubation at 70°C for 10 min.
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bioRxiv - Developmental Biology 2021Quote: ... A T7 RNA polymerase binding site with short 5’-tail (aaaaTAATACGACTCACTATAG) was added to reverse primers for transcription using T7 RNA polymerase incorporating DIG labelled ribonucleotides (NEB, Roche). PCR amplified probe templates were confirmed by sanger sequencing.
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bioRxiv - Neuroscience 2021Quote: ... and overlaid on top of 29% Iodixanol solution (25 mM KCl, 5 mM MgCl2, 10 mM Tris pH 8.0, 29% Iodixanol, 1x protease inhibitor (Roche, 11873580001), 0.4 U/ul RNAse inhibitor (Takara ...
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bioRxiv - Developmental Biology 2021Quote: The fourth and third mammary glands were dissected from 5.5 to 6.5-wk-old female mice, minced with scissors and digested in Digestion medium (DMEM/F12, 2mg/ml Collagenase A (Roche), 5μg/ml insulin (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2020Quote: ... fixed embryos were homogenized in 2-5 ml freshly prepared lysis buffer (50 mM Tris pH7.5; 150 mM NaCl; 5 mM EDTA; 0.5 % NP-40; 1.15 % Triton X-100; 1x Roche Complete protease inhibitors) with a Dounce Homogenizer on ice ...
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bioRxiv - Genomics 2020Quote: ... The PCR reaction contained 5 μl of 2X Kappa Hifi Hotstart ReadyMix (Kappa Biosystems, Roche Sequencing and Life Science, USA), 2 μl of 0.2 mg/ml BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... then harvested by centrifugation and resuspended in lysis buffer (50 mM HEPES-KOH pH 7.0, 500 mM NaCl, 5 mM β-mercaptoethanol, 1x Protease Inhibitor Cocktail (Roche)) and lysed by sonication ...
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bioRxiv - Cell Biology 2019Quote: 5 dpf zebrafish larvae were killed on ice and eyes dissected in a solution of 5 mM NaCl with protease inhibitor cocktail tablets (Roche). Eyes were either snap frozen in liquid nitrogen and stored at -80°C or immediately lysed ...
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bioRxiv - Microbiology 2019Quote: ... RNA transcripts of the cloned 5’UTRB7 fragments were produced in vitro from the resulting purified PCR products by using the T7 Transcription kit (Roche) and labeled by using the RNA 3’ end biotinylation kit (Pierce) ...
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bioRxiv - Genomics 2019Quote: ... The supernatants from the digestion and initial decalcification step were purified using a 5 M guanidine-hydrochloride binding buffer with a High Pure Viral Nucleic Acid Large Volume kit (Roche). The extract was eluted in 100 μl of a 10mM tris-hydrochloride ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were lysed in lysis buffer (50 mM HEPES pH 7.5, 300 mM NaCl, 10% glycerol, 2 mM DTT, 30 mM imidazole and complete EDTA-free protease inhibitors cocktail [Roche]) supplemented with 1 mM TCEP ...
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bioRxiv - Genomics 2019Quote: ... 5 ng DNA fragments were pooled and proceeded on with library construction using KAPA Hyper Prep Kit (Kapa Biosystems #KK8504) according to the standard protocol ...
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bioRxiv - Immunology 2021Quote: Stimulated cells were lysed at 4 °C for 10 min in 1% NP-40 lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, protease inhibitor cocktail (Roche #11836170001), 1 mM Na3VO4 ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed at 4 °C for 10 min in 1% NP-40 lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, protease inhibitor cocktail (Roche# 11836170001), 1 mM Na3VO4 ...
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bioRxiv - Neuroscience 2020Quote: ... A single qPCR reaction is made of 5 μL 2xSYBR green mix (LightCycler® 480 SYBR Green I master, Roche), 2 μL H2O ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed using 2-5 times the volume of RIPA lysis buffer supplemented with 1x protease inhibitor cocktail (Roche), PMSF (1 mM ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting cDNA was used as a template for qRT-PCR using 5× HOT FIREPol qPCR Mix (Solis BioDyne) on the LightCycler 96 System (Roche). Relative expression values were calculated with the 2-ΔΔCt method (Livak and Schmittgen ...
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bioRxiv - Plant Biology 2021Quote: ... qPCR reactions were performed in a 10-μl volume containing 5 μl of LightCycler 480 SYBR Green I Master (Roche), 4 μl of the corresponding primer pair (1.5 μM each) ...
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bioRxiv - Plant Biology 2021Quote: ... Quantification of transcript levels was carried out using SYBR Green reactions with 5 ng cDNA in a 20 μl volume on a Light Cycler 480 II (Roche) relative to the reference gene UBC10 (POLYUBIQUITIN10 ...
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bioRxiv - Systems Biology 2020Quote: ... The filters were washed with 130 μl 50 mM ammonium bicarbonate and centrifuged again before overnight digestion with 100 μl 5 ng/μl trypsin (Roche) at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... and per sample eight PCR reactions with 4,000 beads each in 50µl using 1µM SMART PCR primer (oligos in supplementary table 5) and the 2x Kapa HiFi Hotstart Ready mix (Roche #07958935001) were performed ...
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bioRxiv - Microbiology 2021Quote: ... the remaining dermis was washed in Ca2+ and Mg2+ free PBS 5 times and incubated in a digestion buffer containing 2 mg/ml collagenase A (Roche), 100 µg/ml of DNase I (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Lungs were cut into small pieces and digested in 5 mL of RPMI containing DNase I (100 μg/mL, Roche), Dispase II (800 μg /mL ...
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bioRxiv - Microbiology 2021Quote: ... Cells were harvested by centrifugation at 3,800 × g for 10 min at 4 °C and resuspended in lysis buffer (50 mM NaH2PO4, 300 mM NaCl, 50 mM imidazole, 5% glycerol and Complete Protease Inhibitor Cocktail Tablet (Roche), pH 8.0) ...
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bioRxiv - Physiology 2020Quote: ... RBC lysis and post-lysis wash buffers contained 5 mM Tris-HCl/pH 8.0 and complete Protease Inhibitor Cocktail tablets (cPIC; Roche, 04693116001), as opposed to sodium-phosphate buffer/pH 7.5 and PMSF and pepstatin A—a modification required to obtain good mass-spectrometry data ...
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bioRxiv - Neuroscience 2019Quote: The brain samples were homogenised in 5 volumes (w/v) of sterile PBS containing cOmplete Mini EDTA-free Protease Inhibitor Cocktail tablets (Roche), sodium fluoride (10 mM ...
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bioRxiv - Plant Biology 2021Quote: ... The abundance of specific transcript was measured by probing 1 μL cDNA by quantitative real-time PCR in a total volume of 10 µl containg 5 μL SYBR-green master mix (Roche), 0.5 μM forward and reverse primer and water ...
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bioRxiv - Microbiology 2020Quote: ... Membranes was incubated secondary antibody for an hour in 5% milk and developed using Lumi-light western blot substrate (Roche) to detect HRP ...
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bioRxiv - Microbiology 2020Quote: ... for 30 minutes at 37°C and 5 µg was converted to cDNA by reverse transcription using Transcriptor reverse transcriptase (Roche) and random hexaoligos (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5% NP-40 and supplemented with RNase inhibitor (Murine, M0314, New England Biolabls) and protease inhibitors cocktail (cOmplete Mini, Roche), and passed through a 25G needle 7 times ...
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bioRxiv - Biophysics 2021Quote: ... The pellet was re-suspended in lysis buffer (50 mM HEPES, 500 mM NaCl, 5% glycerol, pH 7.5) supplemented with cOmplete™ protease inhibitor cocktail (Roche), 10 μg/mL DNAse I ...
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bioRxiv - Biophysics 2021Quote: ... The pellet was re-suspended in lysis buffer (50 mM HEPES, 500 mM NaCl, 5% glycerol, pH 7.5) supplemented with cOmplete™ protease inhibitor cocktail (Roche), 10 μg/mL DNAse I ...
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bioRxiv - Biophysics 2021Quote: ... The pellet was re-suspended in lysis buffer (50 mM HEPES, 500 mM NaCl, 5% glycerol, pH 7.5) supplemented with cOmplete™ protease inhibitor cocktail (Roche) and split into 6 fractions ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted from 58 macro-dissected regions of slides G1-5 in samples UH1-UH16 and 30 regions of slides F1-5 in samples UH17-UH23 and UH25-UH27 (Supplementary Table 2) using the High Pure FFPE RNA isolation kit (Roche). For UH4 ...
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bioRxiv - Immunology 2022Quote: ... Precipitated pellet was then resuspended in 100 μL of RIPA buffer plus laemli supplemented with 5 mM diisopropylfluorophosphate (DFP) and protease inhibitor cocktail (Roche) and heat denaturated for 10 min at 95°C ...
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bioRxiv - Biochemistry 2022Quote: ... samples were incubated with 50 μl mix of 5 μl enzyme and 45 μl labeling solution (TUNEL In situ Cell Death Detection Kit, TMR Red’ from Roche) for 1.5 h at 37°C in a dark humid chamber ...
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bioRxiv - Neuroscience 2022Quote: ... The flow through was discarded and the beads washed 5 times with wash buffer (CL48 diluted ¼ in PBS and supplemented with complete protease inhibitor cocktail (Roche)) and sample buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... then lysed for 10 min on ice with IP lysis buffer (10 mM Tris-HCl pH 7.6, 150 mM NaCl, 0.5% NP-40, 5% Glycerol and Roche protease inhibitor). Lysates were centrifuged at 17,000 × g for 10 min and supernatant incubated overnight at 4 °C with 2 μg of anti-PTBP1 antibody (monoclonal-ThermoFisher ...
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bioRxiv - Pathology 2022Quote: ... and brain tissues were homogenized using a Teflon-glass homogenizer in ice-cold homogenization buffer (0.32 M sucrose, 5 mM EDTA, 10 mM Tris-HCl, and pH 7.4, phosphatase inhibitor cocktail tablet; Roche, Mannheim, Germany), centrifuged at 4500 rpm for 10 min at 4°C and the supernatants were collected.
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bioRxiv - Biochemistry 2022Quote: ... purified Q23-huntingtin (5 µg) was purified in the presence of complete protease and phosphatase inhibitor cocktails (Roche Applied Science) and incubated with or without PRKACA (20 ng) ...
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bioRxiv - Molecular Biology 2022Quote: ... Yeast pellets of approximately 50 OD600 units in size were resuspended in 600 µL of lysis buffer (5% SDS, 50mM TEAB, supplemented with protease and phosphatase inhibitor tablets (Roche)) and the 2 mL screwcap tube was filled to capacity with acid-washed glass beads ...
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bioRxiv - Neuroscience 2022Quote: ... homogenized on ice in 5 volumes (w/v) of T-per extraction buffer complemented with protease inhibitor tablets (Complete Mini Protease Inhibitor Tablets, Roche) and phosphatase inhibitor cocktail tablets (phosSTOP ...
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bioRxiv - Cell Biology 2022Quote: ... 30 s at 72°C) and final extension 5 min at 72°C using KAPA Taq DNA polymerase (KAPA BIOSYSTEMS). PCR products were visualized on 2% agarose gels containing SYBR Safe DNA gel stain (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.5 and various concentrations of ADP-ribose or hit compounds were mixed on ice in 384-well PCR plate (Roche). Fluorescent signals were measured from 25 to 95 °C in 0.2 °C/30/Sec steps (excitation ...
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bioRxiv - Cell Biology 2022Quote: ... Glycerol was added to a final concentration of 10% as well as Turbonuclease (5 µL) and a protease inhibitor cocktail tablet (Roche). The cells were lysed by sonication (70% amplitude,10 s pulse/10 s pulse off for 2.5 min) ...