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Citations for Roche :
1301 - 1350 of 7954 citations for 6H Imidazo 4 5 1 de acridin 6 one 5 3 diethylamino propyl amino 8 hydroxy since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Pellets were resuspended in microsome buffer (50 mM MOPS, 0.5 M sorbitol, 5 mM EGTA, complete protease inhibitor (Roche)) and repelleted by centrifugation at 100□000 g for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... was purified via Immobilized Metal Affinity Chromatography (IMAC) using a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HEPES-buffered saline buffer (HBS ...
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bioRxiv - Microbiology 2022Quote: ... and the right inferior lung lobes were digested in 50 μL of 5 mg/mL of Liberase Tm (Roche) and 12.5 μL of 10 mg/mL of DNase I (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2023Quote: ... Up to 5 ng plasma cfDNA (~0.5 mL plasma) was used for cfWGS library with Kapa HiFi Hotstart ReadyMix (Roche) in 11-13 cycles ...
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bioRxiv - Plant Biology 2023Quote: ... 5% (vol/vol) glycerol and 20 mM imidazole (buffer A) supplemented with cOmplete EDTA-free protease inhibitor tablets (Roche) and lysed by sonication ...
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bioRxiv - Molecular Biology 2023Quote: A 20% w/v homogenate was prepared with a piece of snap-frozen liver tissue in homogenization buffer (50 mM Tris-HCl [pH 7.4], 250 mM sucrose, 25 mM KCl, 5 mM MgCl2, 3mM imidazole, supplemented with protease inhibitors [Roche]). To remove nuclei and debris ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM EDTA, 20 mM HEPES, 0.2% NP-40, 10% glycerol, 400U Ribolock inhibitor, 1x protease/phosphatase inhibitor, Roche). Lysates were centrifuged (12000 rpm ...
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bioRxiv - Genomics 2023Quote: ... resuspended in cold lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 0.1 mM EGTA, Protease Inhibitor (Roche, 04693159001)) and incubated on ice for 10’ to isolate the cell nuclei ...
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bioRxiv - Cell Biology 2023Quote: ... 50 mM NaCl, 5 mM EDTA, 50 mM Tris at pH 7.4 and cOmplete EDTA-free Protease Inhibitor Cocktail [Roche]). After agitation at 4°C for 30 min ...
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bioRxiv - Genomics 2023Quote: ... Library construction was performed on the 5 ng of pooled PCR amplicons using the KAPA HyperPrep Kit (Roche 07962363001) and NEXTFLEX Unique Dual Index Barcodes (7.5µM final concentration ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting cell pellet was resuspended in a lysis buffer of Buffer A + 0.5% Triton X-100 containing 5% protease inhibitor cocktail cOmplete (Roche, 118614500 ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 95°C for 5 min in SDS lysis buffer (5% SDS in 100 mM triethylammonium bicarbonate pH 8.5, complete EDTA free protease inhibitor cocktail (Roche), phosphatase inhibitor cokatail-2 ...
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bioRxiv - Neuroscience 2023Quote: ... cells and sections were processed for BrdU immunodetection following the manufacturer’s recommendations (5-Bromo-2′-deoxy- uridine Labelling and Detection Kit I, Roche). The slides were mounted for fluorescent microscopy in ProLong® Diamond Antifade Mountant with DAPI (Life Technologies).
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bioRxiv - Biochemistry 2023Quote: ... and individually transferred in 1.5 ml vials filled with 5 volumes (V/W) of ice-cold 25 mM Hepes pH=7.4 in PBS completed with protease (Mini, Roche) and phosphatase (Phos-stop ...
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bioRxiv - Immunology 2023Quote: ... 2019) by harvesting lung lobes into 5 ml HBSS with 40µl Liberase Tm (0.1 wU/ml, Roche, Cat# 5401127001) and 20µl DNAse 1 (10mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... Each 5 μl reaction contained 2.5 μl of LightCycler 480 SYBR Green I Master mix 2X (Roche Applied Science), 0.5 μl of each primer (300 nM final ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 mM EDTA, 0.1 % NP-40, 20 mM KCl, 150 mM NaCl, plus a protease inhibitor cocktail from Roche) and centrifuged for 2 minutes and 2,500 g at 4 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and 1X Triton buffer (100mM Tris-HCl, pH8.8, 100mM NaCl, 5mM EDTA and 5% Triton-X) with protease inhibitors (11836170001, Roche) for 16 hours at 4° C ...
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bioRxiv - Biochemistry 2023Quote: ... then the pellet was resuspended in 40 ml binding buffer (50 mM NaPi, 300 mM NaCl, 5 mM imidazole, pH 7.5) containing protease inhibitor (Roche cOmplete EDTA-free protease inhibitor cocktail ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was thawed in two volumes of lysis buffer (3M NaCl, 100mM Tris pH7,5, 10% Glycerol, 0.5mM EDTA, 5 mM β-mercaptoethanol, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
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bioRxiv - Cell Biology 2023Quote: ... 5 % glycerol and 0.5 mM TCEP supplemented with 5 Units/mL Benzonase and an EDTA-free protease inhibitor cocktail (Roche)) ...
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bioRxiv - Molecular Biology 2023Quote: ... The pellet was thawed in an equal volume of lysis buffer (1M NaCl, 25mM Tris pH7,5, 5% Glycerol,1mM DTT, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
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bioRxiv - Molecular Biology 2023Quote: ... The pellet was thawed in two volumes of lysis buffer (3M NaCl, 100mM Tris pH7,5, 10% Glycerol, 0.5mM EDTA, 5 mM β-mercaptoethanol, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
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bioRxiv - Physiology 2024Quote: ... Sections were dried and denatured for 10 min at 80°C in hybridization buffer (0.1M Tris, pH 7.2, 25mM MgCl2, 70% formamide [Sigma-Aldrich, Saint Louis, MO], 5% blocking reagent [Roche] with 1.0 μg/mL of Cy-3-labeled telomere-specific [CCCTAA] peptide nucleic acid [PNA] probe [TelC-Cy3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The amplification of the oligonucleotide pool was performed with ∼5 ng of template DNA and KAPA HiFi polymerase (Roche) with the annealing temperature at 53°C and extension time of 15 sec for 14-20 cycles ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was purified from these cells by freeze-thaw lysis in Hepes buffer (20mM Hepes; pH 7.5, 150mM NaCl, 5% glycerol) with Complete protease inhibitor tablets (Roche) or Phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Neuroscience 2024Quote: ... .5 µL of a 10 µM primer mix and 7.5 µL of Fast Start SYBR Green Master Mix (Roche). Each reaction was performed in triplicate ...
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bioRxiv - Neuroscience 2024Quote: ... 5-diphenyl tetrazolium bromide (MTT) in accordance with the manufacturer’s recommendations (Cell Proliferation Kit I; Roche Diagnostics, Basel, Switzerland). In brief ...
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bioRxiv - Molecular Biology 2024Quote: ... 150 mM NaCl, 0.5% NP40, 0.5% Triron-X100, 5% glycerol, 1x Roche Complete EDTA free inhibitor cocktail, 1x Roche PhosSTOP) for 1h on ice by agitation ...
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bioRxiv - Neuroscience 2024Quote: ... The template plasmid was diluted to 5×107 copies/µL with nuclease-free water plus yeast RNA (Roche, 10109223001), and this solution was further diluted to 2.5 ...
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bioRxiv - Cell Biology 2024Quote: ... containing 5% beta-mercaptoethanol and protease and phosphatase inhibitors (Roche Complete Mini protease inhibitor, Sigma-Aldrich #11836153001, and Roche Complete Ultra phosphatase inhibitor ...
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bioRxiv - Microbiology 2024Quote: ... with their respective dilution in 5% skimmed milk in PBS-tween 0.1% were used: anti-HA-HRP (3F10) (N°12013819001, Roche) 1/10,000 ...
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bioRxiv - Neuroscience 2024Quote: ... underwent lysis through the addition of 200 µl of a 50 mM Tris-HCl buffer (pH 7.8) containing 5% SDS and cOmplete ULTRA protease inhibitor (Roche). The samples were then subjected to Bioruptor® treatment (Diagenode ...
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bioRxiv - Molecular Biology 2021Quote: ... washed in cold PBS and incubated on ice in cold swelling buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.2% NP-40, 1 mM AEBSF and 1x complete mini EDTA-free proteasome inhibitor, Roche) for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then vigorously resuspended in 1ml of fresh ice-cold lysis buffer (50mM Tris pH 8, 150mM NaCl, 0.5% NP-40, 1 tablet of complete protease inhibitor (04693159001, Roche)) and transferred to 9 ml of pre-chilled lysis buffer and incubated for 20 min on ice ...
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bioRxiv - Immunology 2021Quote: ... Pellets of cross-linked cells were resuspended in shearing buffer (10 mM Tris-HCl pH 8, 0.1% SDS, 1 mM EDTA) supplemented with Complete Protease Inhibitor Cocktail (Roche), and chromatin was sheared to an average fragment size of 200-250 bp ...
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bioRxiv - Molecular Biology 2020Quote: ... and subjected to 8 cycles of lysis in a MagNALyser (Roche – 6000 rpm, 1 min on, 2 min off). Lysate was transferred to fresh microfuge tubes and clarified by centrifugation (13,000 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... 10mM Tris-HCl pH 8, 1 mM DTT, 0.2% NP-40, 1x protease inhibitor [Roche], 1x phosphatase inhibitor [Roche]), sonicated and centrifugated for 10 minutes at 14,000 RPM at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Released nuclei were washed (10 mM Tris-HCl pH 8, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1x PIC (Roche)) for 5 minutes at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Isolated nuclei were then lysed with lysis buffer (50mM Tris-HCl, pH 8.1, 10 mM EDTA, pH 8, 1% SDS, 1X protease inhibitor cOmplete EDTA-free cocktail, Roche). Nuclei were sonicated using a Bioruptor ultrasonic cell disruptor (Diagenode ...
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bioRxiv - Cell Biology 2019Quote: Cells were harvested and lysed in ice-cold lysis buffer (20 mM Tris pH 8, 200 mM NaCl, 1 mM EDTA, 0.5% NP40) with protease inhibitor cocktail (Roche). Protein content of extracts was estimated using Pierce 660 nm Protein Assay Reagent (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... Add 120 ul of fresh ChIP SDS Lysis Buffer (0.5% SDS, 50 mM Tris, pH 8, 10 mM EDTA, 1 × protease inhibitor cocktail (Roche)) per 5 million cells ...
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bioRxiv - Immunology 2022Quote: ... were lysed in 400 μl lysis buffer in urea buffer (8 M urea, 50 mM Tris pH 8.0, 1 mM TCEP) with protease (cOmplete mini EDTA free, Roche) and with phosphatase inhibitors (PhosStop ...
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bioRxiv - Cell Biology 2022Quote: ... The pellet was resuspended with 20 mL lysis buffer (50 mM NaH2PO4, 0.5 M NaCl, pH 8) and 1 cOmplete Mini Protease Inhibitor Cocktail tablet (Roche) per 1 L of expression ...
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bioRxiv - Plant Biology 2023Quote: ... in 100μl GTEN-buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl,1 x protease inhibitors (Roche), 5mM DTT ...
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bioRxiv - Plant Biology 2023Quote: ... A GTEN-based buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl, 1 x protease inhibitors (Roche), 5mM DTT ...
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bioRxiv - Physiology 2022Quote: ... and resuspended in 600 μL of cold nuclear lysis buffer (10 mM Tris pH 8, 1 mM EDTA, 0.5 mM EGTA, 0.3% SDS, 1X cOmplete™ Protease Inhibitor Cocktail, Roche) for 30 min on ice ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed in IP buffer (10 mM Tris pH 8, 0.4% NP40, 300 mM NaCl, 10% glycerol, 1 mM DTT, antiprotease (Roche), anti-phosphatase (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... washed with 1 x PBS and resuspended in 100 μl of SoTE buffer (10 mM Tris-HCl pH 8, 1 mM EDTA, 0.15 % Triton X-100, protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cancer Biology 2024Quote: ... or protein extraction (50 mM Tris-HCl, 1% NP40, 0.15 M NaCl, pH 8) supplemented with phosphatase (PhoStop, Roche) and protease inhibitors (Complete ...