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Citations for Roche :
1251 - 1300 of 7954 citations for 6H Imidazo 4 5 1 de acridin 6 one 5 3 diethylamino propyl amino 8 hydroxy since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Supernatants were diluted 1:4 with ice-cold dilution buffer (1× phos-stop [Roche], 0.5% NP-40, 1 mM DTT) and centrifuged again at 15,000 rpm for 15 minutes at 4°C to precipitate actomyosin components ...
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bioRxiv - Molecular Biology 2020Quote: ... and then deproteinized by adding of 8 µl of 10% SDS and 8 µl of proteinase K (19 mg/ml stock, Roche). Deproteinization was carried out at 37 °C for 30 min with gentle flicking ...
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bioRxiv - Molecular Biology 2019Quote: ... ~2 x 108 parasites were resuspended in 2 mL cytoplasmic lysis buffer (10 mM HEPES [pH 8], 10 mM KCl, 0.1 mM EDTA [pH 8], plus complete protease inhibitor [PI, Roche # 11836170001]), transferred to a prechilled 2 mL douncer homogenizer and set on ice for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... grown for 24 hours and transfected with 8 μg of plasmids and 8 μl of X-tremeGENE HP DNA Transfection Reagent (Roche). The cells were grown for 48 hours post-transfection and collected for downstream analyses.
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bioRxiv - Cell Biology 2022Quote: ... Cells were harvested using ice cold EBC buffer (140 mM NaCl, 50 mM Tris-HCl, 0.5% NP-40, 5 mM EDTA and protease inhibitor cocktail (Roche)) and briefly sonicated prior to 20 min centrifugation at 20,000 g ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mM EDTA) with the addition of a Complete EDTA free protease inhibitor cocktail tablet (Roche Diagnostics, Oslo, Norway) and a PhosSTOP tablet (Roche) ...
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bioRxiv - Neuroscience 2019Quote: ... cells were collected and lysed with a lysing buffer (100mM KCl, 20mM HEPES, 5% Glycerol, 0.1X Triton-X, 1mM DTT, and Complete protease inhibitor (Roche)) ...
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bioRxiv - Neuroscience 2019Quote: ... Resin was washed with TBS buffer (150 mM NaCl, 20 mM Tris-Cl, pH 7.4, 5 mM MgCl2, EDTA free protease inhibitors (Complete, Roche) and incubated with purified 6xHis-tag LC3 for 120 min @4°C in TBS buffer either alone or in the presence of purified 6xHis-Snapin ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 mM KCl, 5 mM MgCl2, 0.01% NP-40, freshly prepared 20 mM Na2MoO4, and cOmplete protease inhibitors; Roche). 10-20 million cells were lysed in 0.5 mL Felts buffer by rotation at 4°C for 30 minutes and vortexing for 10 seconds ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei pellets were resuspended in 1 ml nuclei permeabilization buffer (5 % BSA, 0.2 % IGEPAL-CA630, 1mM DTT and cOmpleteTM, EDTA-free protease inhibitor cocktail (Roche) in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... which were blocked and permeabilized with 5% normal goat serum in 0.25% Triton X-100 in PBS (Roche Diagnostics) for 30 min at 25°C ...
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bioRxiv - Plant Biology 2021Quote: ... Each 10 μl reaction was comprised of 5 μl LightCycler 480 SYBR Green I Master mix (Roche Life Science), 2 μl cDNA template ...
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bioRxiv - Biochemistry 2021Quote: ... Pelleted cells were resuspended in 1x PBS buffer (pH 7.4) with 5 mM BME and proteinase inhibitor cocktail (Roche). For purification of PABPC1-His ...
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bioRxiv - Microbiology 2020Quote: ... cells were centrifuged and pellets were resuspended in RB1 (20mM Tris pH7.5, 300mM NaCl, 1mM DTT, 5% glycerol and protease inhibitor (complete EDTA-Free, Roche)) ...
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Plant pathogens convergently evolved to counteract redundant nodes of an NLR immune receptor networkbioRxiv - Plant Biology 2021Quote: ... 5% (vol/vol) glycerol and 20 mM imidazole (buffer A) supplemented with cOmplete EDTA-free protease inhibitor tablets (Roche) and lysed by sonication ...
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bioRxiv - Plant Biology 2021Quote: ... 500 nM of each primers and 5 μl of 2X LightCycler® 480 SYBR Green I Master mix (Roche) in 10 μl final volume ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was diluted 5 times and subjected to real-time PCR using LightCycler 480 SYBR Green I Master (Roche) with specific primers for the genes of interest ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei pellets were resuspended in 1 ml nuclei permeabilization buffer (5 % BSA, 0.2 % IGEPAL-CA630, 1mM DTT and cOmpleteTM, EDTA-free protease inhibitor cocktail (Roche) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells from mouse blood infected with the DEH-GFP-expressing parasite were pelleted and then lysed in hypotonic buffer (10 mM Tris-HCl pH 8.4, 5 mM EDTA) containing protease inhibitors (Roche), freeze/thawed twice ...
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bioRxiv - Biophysics 2021Quote: ... The troponin T detection kit (Elecsys® Troponin T Gen 5 STAT) and troponin T were purchased from Roche. 1X phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2021Quote: ... were harvested in ice-cold lysis buffer (0,25% NP40, 5% glycerol, 50 mM Tris HCl, 150 mM NaCl) containing protease inhibitor (Roche), DNAse (5 µl/ml ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were lysed in lysis buffer (30 mM HEPES, pH 7.5, 300 mM NaCl, 5% glycerol, 30 mM imidazole, Complete EDTA-free protease inhibitor [Roche]) and the cleared lysate was incubated with Ni-NTA agarose beads (Qiagen) ...
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bioRxiv - Molecular Biology 2022Quote: ... in lysis buffer (50 mM Tris–HCl pH 7.5, 300 mM NaCl, 10 mM imidazole, 5 mM β-mercaptoethanol, 0.5% Triton X-100, Roche Complete Mini EDTA-free protease inhibitor tablets (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2022Quote: ... filtered conditioned medium was loaded onto a 5 ml cOmplete™ His-tag Purification Column (Roche Diagnostics, Vilvoorde, Belgium). The protein was eluted with 250 mM imidazole in HBS after which the imidazole was removed using a HiPrep™ 26/10 Desalting column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... PSH in DDM micelles or in POPC vesicles was diluted with MES-buffer (50 mM MES pH 6.0, 500 mM NaCl, 5 mM MgCl2, 1x PI mix complete (Roche)) to 1 - 2 µM PSH ...
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bioRxiv - Biochemistry 2022Quote: ... pelleted cells were resuspended in lysis buffer (20 mM Tris-HCl pH 7.5, 500 mM NaCl, 5 mM MgCl2 with EDTA-free protease inhibitor (Roche)) ...
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bioRxiv - Developmental Biology 2021Quote: ... resuspended in cold lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 0.1 mM EGTA, Protease Inhibitor (Roche, 04693159001)) and incubated on ice for 10’ to isolate the cell nuclei ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was clarified by a 5 µm filter followed by a 0.45 µm filter and loaded on a cOmplete His-Tag purification Column (Roche) with a column volume (CV ...
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bioRxiv - Microbiology 2020Quote: ... Mycelia pellets were thawed on ice and resuspended in 0.4 mL ice-cold lysis buffer (20 mM Tris pH 8.0, 5 mM EDTA, 1x EDTA-free protease inhibitors [Roche]) and sonicated (5x 15 sec on/15 sec off at 5-micron amplitude) ...
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bioRxiv - Molecular Biology 2019Quote: ... The E.coli cells were resuspended in lysis buffer (20mM HEPES, pH 7.5, 500mM NaCl, 5% glycerol supplemented with 1X PIC (Roche), 100ug/ml lysozyme and lysed by sonication and centrifuged ...
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bioRxiv - Microbiology 2019Quote: ... Transfected cells were washed two times in cold PBS and lysed in lysis buffer (20 mM Hepes, 100 mM NaCl, 5 mM MgCl2, 0,2 % Triton X100, 10 % Glycerol, protease inhibitors (Roche)) ...
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bioRxiv - Molecular Biology 2019Quote: ... up to 5 ng/sample of ChIP was prepared for sequencing using the Kapa Hyper-prep Kit (Kapa Biosystems) using NEXTflex adapters (Bio Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... frozen HEK293E cells were thawed and resuspended in 2x excess of lysis buffer (25 mM HEPES-KOH pH 7.6, 5 mM MgCl2, 50 mM KCl, cOmplete protease inhibitor cocktail (Roche), 1 mM PMSF ...
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bioRxiv - Genetics 2020Quote: ... 721-bp tandem and 1600-bp tandem repeats were selected and PCR labelled with tetramethyl-rhodamin-5-dUTP (Roche) (Ishii et al ...
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bioRxiv - Biochemistry 2020Quote: ... cells were lysed in Buffer A (2 M NaCl, 20 mM HEPES pH 7.5, 20 mM Imidazole, 5 mM βME, protease inhibitor (Roche)) by sonication ...
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bioRxiv - Microbiology 2019Quote: ... cells were lysed in ice-cold lysis buffer (10 mM Tris pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, supplemented with a protease cocktail inhibitor from Roche, Switzerland) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2019Quote: ... cortices from 6-week old mice were homogenized in cold sucrose buffer (20 mM HEPES pH 7.4, 320 mM sucrose, 5 mM EDTA) with protease inhibitor cocktail (Roche). Homogenates were centrifuged at 3,000 g for 20 min at 4°C speed to pellet nuclei ...
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bioRxiv - Physiology 2021Quote: ... Sections were dried and denatured for 10 min at 80°C in hybridization buffer (0.1M Tris, pH 7.2, 25mM MgCl2, 70% formamide [Sigma-Aldrich, Saint Louis, MO], 5% blocking reagent [Roche] with 1.0 μg/mL of Cy-3-labeled telomere-specific [CCCTAA] peptide nucleic acid [PNA] probe [TelC-Cy3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Dechorionated embryos were then washed once with cold DEI buffer (10 mM HEPES pH 7.4, 5 mM DTT and 1x complete protease inhibitor from Roche) followed by homogenization in 1-2 embryo volumes of DEI buffer on ice ...
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bioRxiv - Biochemistry 2021Quote: ... 20 mM imidazole and 5 mM β-mercaptoethanol (BME)) and supplemented with DNase I and protease inhibitor cocktail (Roche). Cells were disrupted by Emulsiflex C3 (Avestin ...
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bioRxiv - Genomics 2019Quote: ... Nuclei pellets were resuspended in 1 ml nuclei permeabilization buffer (5 % BSA, 0.2 % IGEPAL-CA630, 1mM DTT and cOmpleteTM, EDTA-free protease inhibitor cocktail (Roche) in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... 5 ml NP-40 in a total volume of 500 ml ddH2O) containing phosphatase and protease inhibitor cocktail (Roche) then centrifuged for 30 min at 12000 rpm at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... All cortices were homogenized in cold sucrose buffer (20 mM HEPES pH 7.4, 320 mM sucrose, 5 mM EDTA) supplemented with protease inhibitor cocktail (Roche) and a BCA was perform to assess the protein concentration of each sample ...
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bioRxiv - Neuroscience 2022Quote: ... the neocortex from 8- 10 six-week-old mice was homogenized in cold sucrose buffer (20 mM HEPES pH 7.4, 320 mM sucrose, 5 mM EDTA) supplemented with protease inhibitor cocktail (Roche). Homogenates were centrifuged at low speed to pellet nuclei and cell debris (3,000 g for 20 min at 4_C ...
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bioRxiv - Developmental Biology 2022Quote: ... DIG-labelled cDNA probe was incubated at 95 °C for 10 min and immediately placed in the ice for 5 min before adding to the hybridization buffer (5x SSC, 50 % formamide, 0.02 % SDS, 2 % blocking agent (Roche), DEPC-treated water) ...
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bioRxiv - Biochemistry 2022Quote: ... The pellet was suspended in resuspension buffer (5% glycerol, 20 mM HEPES pH 7.4, 200 NaCl, cOmplete protease inhibitor mixture (Roche)) at a concentration of 4 mg/mL using a tissue grinder ...
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bioRxiv - Biophysics 2022Quote: ... constructs were resuspended in Buffer C (20 mM at Tris pH 7.6, 300 mM NaCl, 5 mM DTT, and Roche Complete Mini protease inhibitor cocktail (Roche Diagnostics 11836153001)) ...
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bioRxiv - Plant Biology 2023Quote: ... 5% (vol/vol) glycerol and 20 mM imidazole (buffer A) supplemented with cOmplete EDTA-free protease inhibitor tablets (Roche) and lysed by sonication ...
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bioRxiv - Neuroscience 2022Quote: ... Each region was homogenized using Ribolyser for 5 min in 500uL lysis buffer (140mM NaCl, 20mM TrisHCl, pH 7.5, protease inhibitors [complete Mini, Roche] ...
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bioRxiv - Neuroscience 2023Quote: ... Hematocrit (0.5 to 0.64) and blood glucose concentration (5.7 to 8.6 mmol/L, Accu-Chek Performa Nano, Roche, Mannheim, GER) were measured shortly before injecting the radiotracer.