Labshake search
Citations for Roche :
9951 - 10000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Digoxigenin (DIG)-labeled sense and antisense riboprobes were prepared using the in vitro transcription DIG labeling kit (Roche, IN, USA). Hybridization was carried out at 600C overnight and the slides were washed and probed with an alkaline phosphatase-conjugated anti-DIG antibody (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... according to the manufacturer’s instructions (Roche, Mannheim, Germany). At least three samples were analyzed per genotype ...
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bioRxiv - Developmental Biology 2023Quote: ... and complimentary DNA libraries were prepared using the KAPA RNA HyperPrep Kit (Roche) according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 5µL SYBR Green I Master mix (5 mL: Roche Diagnostics, Indianapolis, IN) and 2 µL of distilled and deionized H2O ...
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bioRxiv - Developmental Biology 2023Quote: ... qPCR was performed on white 384-well plates (E&K Scientific, Santa Clara, CA) using Roche Lightcycler 480 (Roche Molecular Systems, Inc.). Each reaction contained 1 µL of cDNA from the RT reaction ...
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bioRxiv - Developmental Biology 2023Quote: 970 µg/mL Insulin (11376497001, Roche), 535 µg/mL holotransferrin (T0665 ...
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bioRxiv - Developmental Biology 2023Quote: ... that were conjugated with an alkaline phosphatase (Roche) for colorimetric ISH or horse-radish peroxidase (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... and nitro blue tetrazolium chloride (NBT, [Roche]) in alkaline phosphatase (AP ...
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bioRxiv - Developmental Biology 2023Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was done following manufacturer’ s instruction (In Situ cell Death Detection Kit TMR red, Roche).
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bioRxiv - Developmental Biology 2023Quote: ... probes were detected using anti-digoxigenin and anti-fluorescein antibodies with Fab fragments conjugated to horseradish peroxidase (Roche). 1:500 fluor-tyramide (TAMRA or FAM) ...
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bioRxiv - Developmental Biology 2023Quote: ... 5-Bromo-4-chloro- 3-indolyl phosphate (BCIP, [Roche]) and nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Developmental Biology 2023Quote: ... Antisense probes were synthesized with digoxigenin-11-UTP (Roche) or fluorescein-12-UTP (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... or fluorescein-12-UTP (Roche) using standard molecular protocols (Collins et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... for colorimetric ISH or horse-radish peroxidase (Roche) for fluorescent ISH (FISH) ...
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bioRxiv - Developmental Biology 2023Quote: ... supplemented with Mini EDTA-free protease inhibitor cocktail mix (Roche, CH). Proteins were separated by electrophoresis and transferred onto 0.2μm nitrocellulose membranes (Bio-Rad Laboratories ...
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bioRxiv - Developmental Biology 2023Quote: ... T7 polymerase and biotin labeling mix (Roche #11685597910) were used according to the package directions ...
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bioRxiv - Developmental Biology 2023Quote: ... Crossing point (Cp) values were calculated by LightCycler 480 Software (Roche) using the second-derivate maximum algorithm ...
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bioRxiv - Developmental Biology 2023Quote: ... qRT-PCR reactions were run in a LightCycler 480 Instrument (Roche) using a 5 μl reaction mixture of DNA SYBR Green I Master (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... using a 5 μl reaction mixture of DNA SYBR Green I Master (Roche) according to the standard manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... and KAPA library quantification kit (Roche Cat# 0796014001.) Gene expression libraries were sequenced at a targeted depth of 25,000 reads per cells ...
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bioRxiv - Immunology 2023Quote: Cells were collected and lysed in 2x RIPA buffer + 2x Protease Inhibitors (Roche #4693159001). The protein concentration was determined with the Pierce™ BCA Protein Assay Kit (ThermoFisher #23225 ...
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bioRxiv - Immunology 2023Quote: ... The epidermis and dermis layers were separated after incubation at 37°C for 1h in 0.4 mg/mL dispase II (Roche). The epidermis was collected and then cut into small pieces using scalpels and incubated for 30 minutes with agitation at 37°C in RPMI containing 10% FCS and 0.5 mg/mL DNAse I ...
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bioRxiv - Immunology 2023Quote: ... The epidermis and dermis layers were separated after incubation at 4°C for 16h in 0.2 mg/mL dispase II (Roche). Epidermal layers were fixed in 4% Para-formaldehyde for 20 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Transcripts were quantified by real time PCR on a 480 LightCycler instrument (Roche). Reactions were carried out in 10μL ...
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bioRxiv - Immunology 2023Quote: ... Lamina propria digestion was performed using 0.1 mg/mL Liberase TL (Roche #540102001) and 0.5 mg/mL DNAse (DN25 ...
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bioRxiv - Immunology 2023Quote: ... with proteinase inhibitor (Roche) and phosphatase inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: Metabolic activity was determined using XTT Cell proliferation kit II (Roche, #11465015001). Cells were seeded at a density of 0.3 x 106 (PMC-306 ...
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bioRxiv - Immunology 2023Quote: Apoptosis was analyzed with terminal deoxynuclotidyltransferase-mediated dUTP nick-end labeling (TUNEL; Roche) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Approximately 500ng Total RNA was used for RNA-seq library preparation by following the KAPA Stranded RNA-Seq Kit with RiboErase (HMR) sample preparation guide (Cat. No: KR1151, KAPA Biosystems, USA). The first step in the workflow involved the depletion of rRNA by hybridization of complementary DNA oligonucleotides ...
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bioRxiv - Immunology 2023Quote: ... 1 µg of RNA was reverse transcribed using random oligonucleotides (Roche, #11034731.001) and Omniscript Reverse Transcription (RT ...
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bioRxiv - Immunology 2023Quote: 50,000 sorted cells were washed with PBS and resuspended in Buffer 1 (1X eBioscience Perm/Wash Buffer, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Immunology 2023Quote: ... and 0.1 mg/ml of DNase I (Roche). Samples were incubated for three times at 37 °C for 20 min and constant shaking (2,000 rpm) ...
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bioRxiv - Immunology 2023Quote: ... Tissue was digested in RPMI 1640 containing 1.25 mg/ml collagenase D (Roche), 0.2 mg/ml of Dispase II (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... and incubated on ice for 1 hr and washed twice with Buffer 2 (0.05% w/v Saponin, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Immunology 2023Quote: ... Illumina sequencing libraries were prepared using the KAPA HTP Library Preparation Kit (KAPA Biosystems #KK8234) according to the manufacturer’s instructors with <0.001-3.8 ng input DNA and 14 cycles of PCR ...
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bioRxiv - Immunology 2023Quote: ... supplemented with protease and phosphate inhibitor tablets (Roche)) ...
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bioRxiv - Immunology 2023Quote: ... The gut was then added to a 1.5 mL Eppendorf tubed filled with 1 mL pre-warmed 37°C collagenase/dispase solution (Roche 10 269 638 001) and incubated for 90 seconds before adding to a 40-micron filter as above ...
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bioRxiv - Immunology 2023Quote: ... lysed by ice-cold RIPA buffer (50 mM Tris pH8, 150 mM NaCl, 2 mM EDTA, 0.5% NP40) supplemented with protease inhibitors (Roche) and by sonication ...
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bioRxiv - Immunology 2023Quote: ... FastStart Universal SYBR Green Master (Roche) was used for qPCR and was performed on StepOnePlus™ Real-Time PCR System (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Oligonucleotides used in this study were supplied by Roche Diagnostics (Sant Cugat del Vallès ...
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bioRxiv - Immunology 2023Quote: ... 50μM beta-mercaptoethanol and 10 ng/ml recombinant human IL-2 (Roche). Both human and murine CD4+ T cells were cultured for 46 hrs under humidified conditions at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Following antibodies were diluted in 1-5% BSA (Roche): anti-cGAS (Cell Signaling ...
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bioRxiv - Immunology 2023Quote: ... 2X complete protease cocktail inhibitor (Roche), 2X protease and phosphatase inhibitor (Pierce ...
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bioRxiv - Immunology 2023Quote: ... and 10 ng/ml recombinant human IL-2 (Roche). For activation of murine CD4+ T cells ...
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bioRxiv - Immunology 2023Quote: ... EDTA-free protease inhibitor (Roche 11836 170 001), and 60 U/mL RNasin-Plus RNase Inhibitor (Promega N2611) ...
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bioRxiv - Immunology 2023Quote: ... 1 x EDTA-free protease inhibitor (Roche) and 0.1% Triton-X100 (Merck ...
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bioRxiv - Immunology 2023Quote: ... and 2000 U/mL DNAse I (Roche). These digestion tubes were placed in a shaking incubator (37°C ...
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bioRxiv - Immunology 2023Quote: ... minced with razor blade and digested enzymatically with 3 mg/mL collagenase/dispase (Roche) at 37 °C for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... Fat was digested enzymatically with 3 mg/ml collagenase/dispase (Roche) at 37°C for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... and 40μg/ml DNase I (Roche, UK) for 45 - 60min at 37°C ...