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Citations for Roche :
9851 - 9900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: The library preparation method is a combination of KAPA HyperPrep and HyperPlus (Roche), which enables both high molecular weight DNA ...
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bioRxiv - Genomics 2023Quote: ... and libraries were prepared using the Kapa HyperPrep Library Construction kit (cat. KK8504, Roche Diagnostics). Whole genome sequencing was performed to 30x coverage for Luc-MC26 and 15x coverage for Balb/c buffy coat ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed in lysis buffer (2% SDS in 50 uM Tris-HCL pH.8, supplemented with EDTA-free protease inhibitor, Roche) at cell concentration of 20×106/mL and incubated at 95 °C for 15 minutes ...
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bioRxiv - Genomics 2023Quote: ... 1X protease inhibitor cocktail (Roche) and 10 mM EDTA was added ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted using phenol-chloroform extraction method and 40 μg of glycogen (Roche cat. no. 10901393001) was mixed with the aqueous phase ...
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bioRxiv - Immunology 2023Quote: ... the common bile duct was clamped and the pancreatic duct was perfused with 3-5 mL solution of collagenase P in HBSS-1% HEPES (Roche). The pancreas was then harvested and transferred to a 50mL conical tube containing 5mL of collagenase P solution and kept on ice until all organs were collected ...
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bioRxiv - Immunology 2023Quote: ... LiberaseTM (100 µg/mL, Roche, Indianapolis, IN, USA), hyaluronidase 1a (1 mg/mL ...
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bioRxiv - Immunology 2023Quote: ... supplemented with protease inhibitors (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail, Roche) and 1mM PMSF (Cell Signaling Technology).
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bioRxiv - Immunology 2023Quote: ... Mice were tested weekly and considered diabetic after two consecutive blood glycemia readings of > 250 mg/dL using an ACCU-CHEK blood glucose monitor (Roche).
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bioRxiv - Immunology 2023Quote: Lungs were perfused with sterile PBS and digested for 45 minutes with 625μg/mL collagenase D (Roche 11088875103) and 75U/mL DNase I (Sigma D4527) ...
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bioRxiv - Genomics 2023Quote: Cell-free fDNA and gDNA libraries were constructed using the Kapa Hyper Prep kit (cat: 07962363001, Roche) with custom dual index duplex UMI adapters (IDT) ...
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bioRxiv - Immunology 2023Quote: ... 12.5 μl of SYBR Green (Roche), 0.75 μl of the forward and reverse primer (OriGene ...
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bioRxiv - Immunology 2023Quote: HEK293 cells were seeded at 400,000 cells per well in 6-well plates and transfected the day after with indicated cDNA constructs with FuGENE6 (Roche). Reverse transfection of siRNAs was carried out using RNAiMAX according to the manufacturer’s instructions (Invitrogen Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... Cells were washed twice in cold PBS and scraped off the plates in cold PBS containing protease inhibitors (Roche, Complete 11836170001 and Halt ...
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bioRxiv - Immunology 2023Quote: ... Protease inhibitor cocktail (11697498001) was purchased from Roche. Total RNA extraction kit (8034111 ...
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bioRxiv - Immunology 2023Quote: ... DOTAPs were: D10530 from Psaitong (Beijing) and 11202375001 from Roche. Poly(I:C ...
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bioRxiv - Immunology 2023Quote: ... 20 mM imidazole) and supplemented with protease inhibitor (cOmplete Protease Inhibitor Cocktail, Roche) and benzonase nuclease (Sigma ...
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bioRxiv - Immunology 2023Quote: HEK293 cells were seeded 72 hours prior to the experiment at 400,000 cells per well in 6-well plates and transfected the next day with wt or mutated myc-ALPK1 constructs with FuGENE6 (Roche) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... T237M or V1092A mutated hALPK1 cDNA constructs using FuGENE 6 (Roche).
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bioRxiv - Genomics 2023Quote: ... treated at 55°C and then pre-cleared with 1:2 KAPA Pure beads (Roche Cat. No: 07983298001). The quality and quantity of fragmented DNA were verified using agarose gel (1.5% ...
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bioRxiv - Genomics 2023Quote: Cells were washed in PBS and lysed in RIPA buffer with protease inhibitor (Roche) on ice for 15min ...
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bioRxiv - Genomics 2023Quote: ... Bank validation was done with the quantification of the complementary DNA with the Standard Sensitivity NGS kit on Fragment Analyzer and with qPCR (ROCHE Light Cycler 480). The sequencing was done on NovaSeq 6000 (Illumina ...
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bioRxiv - Genomics 2023Quote: ... The brain samples were homogenized in RIPA with 1% SDS and protease inhibitor cocktail (1 tablet per 10 ml, Roche complete, EDTA-free, Sigma-Aldrich) using a sonicator with a 10 s pulse at 40% amplitude.
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bioRxiv - Genomics 2023Quote: ... 6 μL of Ligation-1 mix (3.75 U T4 DNA ligase (Roche, 10799009001), 33.3 mM DTT (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... in 1X Ligase Buffer (Roche, 10799009001)) was added to a total volume of 26 μL per tube ...
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bioRxiv - Genomics 2023Quote: ... 1 X cOmplete™ protease inhibitor cocktail (Roche, 11697498001), 0.05% Saponin (Sigma ...
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bioRxiv - Genomics 2023Quote: ... 4 μL of Lysis mix (Proteinase K (5.05 mg/mL, Roche, 3115879001), IGEPAL CA-630 (5.05% ...
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bioRxiv - Cell Biology 2023Quote: ... and complete protease inhibitors without EDTA (PIM; Roche)) ...
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bioRxiv - Genetics 2023Quote: ... and phosphatase inhibitor (PhosSTOP Easy, Roche, Basel, Switzerland) was added to each sample ...
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bioRxiv - Genetics 2023Quote: ... The sequencing library was then validated on the Agilent TapeStation and quantified using a Qubit 2.0 Fluorometer in addition to quantitative PCR (KAPA Biosystems, Wilmington, MA, USA). The sequencing libraries were multiplexed and clustered onto two flowcells ...
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bioRxiv - Genetics 2023Quote: ... as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA). The sequencing libraries were clustered on a flowcell ...
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bioRxiv - Genetics 2023Quote: ... with 1x Complete Mini EDTA-free protease inhibitor cocktail tablets (Roche Applied Sciences ...
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bioRxiv - Genetics 2023Quote: ... and analyzed with a Lightcycler 480 (Roche) or LC96 (Roche) ...
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bioRxiv - Immunology 2023Quote: The cell lysis buffer 10 M urea containing the cOmplete™ protease inhibitor cocktail (Roche, #11697498001) was used for protein extraction ...
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bioRxiv - Genetics 2023Quote: ... supplemented with EDTA-free cOmpleteTM Protease Inhibitor Cocktail (Roche), for 20 minutes on ice and centrifuged 14,000g for 10 minutes at 4°C ...
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bioRxiv - Genetics 2023Quote: Mouse tissue was homogenized in 400 μl of RIPA homogenizing buffer (50-mM Tris HCl pH 7.4, 150-nM NaCl, 1-mM EDTA, supplemented with protease-inhibitor cocktails (Roche)) and lysed with a MagNA Lyser ...
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bioRxiv - Genetics 2023Quote: ... gap repaired with KAPA HiFi HotStart Uracil+ Ready Mix (Roche) and treated with a mixture of USER enzyme and T4 polynucleotide kinase (NEB) ...
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bioRxiv - Genetics 2023Quote: ... cDNA was synthesized with Anchored-oligo (dT)18 primers and the Transcriptor First Strand cDNA Synthesis Kit (Roche). cDNA was diluted five-fold with water and 2-4μl of the diluted cDNA served as template for each qRT-PCR as described previously 4 ...
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bioRxiv - Genetics 2023Quote: ... Completed libraries were quantified by qPCR using KAPA Library Quantification kit (Complete kit; Universal) (Roche) and sequenced with 150 bp paired-end reads on an Illumina NextSeq 550 instrument ...
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bioRxiv - Genetics 2023Quote: Quantitative RT-PCR was performed in a final volume of 5 μl with SYBR Green I Master (Roche) and analyzed with a Lightcycler 480 (Roche ...
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bioRxiv - Genetics 2023Quote: ... and amplified for 15 cycles using KAPA HiFi HotStart ReadyMix (KAPA BIOSYSTEMS). Following a final purification with Agencourt AMPure XP beads ...
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bioRxiv - Genetics 2023Quote: ... or LC96 (Roche). For each reaction ...
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bioRxiv - Genetics 2023Quote: ... 2X Complete protease inhibitor cocktail (Roche)) and transferred in 2mL tubes containing glass beads (ozyme ...
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bioRxiv - Genetics 2023Quote: ... The qPCR was performed in a Lightcycler480 qPCR machine (Roche, Basel, Switzerland), with a pre-incubation of 95°C for 3 minutes ...
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bioRxiv - Genetics 2023Quote: ... SensiMix reagents (GCBiotech) and the LightCycler 480 (Roche Diagnostics) were used ...
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bioRxiv - Genetics 2023Quote: The TUNEL assay was performed to detect apoptosis using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and In-Situ cell death detection Fluorescein kit (Roche Cat#11684795910), as published ...
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bioRxiv - Genetics 2023Quote: ... We PCR-amplified bisulfite-treated DNA with 15-16 cycles with KAPA HiFi Uracil polymerase (Kapa Biosystems). We used Illumina TruSeq unique index adapters for PCR amplification and multiplexing of the libraries ...
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bioRxiv - Genetics 2023Quote: ... using a MagNA Lyser (Roche Diagnostics; Woerden, The Netherlands). Concentrations were measured using a Pierce bicinchoninic acid (BCA ...
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bioRxiv - Genetics 2023Quote: ... and the KAPA Library Quantification Kit optimised for Roche® LightCycler 480 (Roche, 07960298001). The multiplexed libraries were sequenced as 150 bp paired-end reads on the Illumina Novaseq 6000 S1 ...
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bioRxiv - Genetics 2023Quote: ... 1% blocking reagent (Roche Diagnostics, Mannheim, Germany), and 35% (v/v ...