Labshake search
Citations for Roche :
9901 - 9950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Cells were washed three times with 1 ml buffer A (15 mM Tris [pH 7.5], 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, one tablet Roche complete EDTA-free mini protease inhibitors ...
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bioRxiv - Genetics 2023Quote: DNA fragments of an Alu sequence with T7 promoter on the 5’ end (ssAlu) or both ends (dsAlu) were in vitro transcribed with the biotin RNA-labeling mix (Roche) and T7 transcription kit (Roche) ...
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bioRxiv - Genetics 2023Quote: ... and T7 transcription kit (Roche). 1 × 107 HEK293T cells were suspended in 1 mL RIP buffer (25mM Tris at pH7.5 ...
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bioRxiv - Genetics 2023Quote: ... for 30 min at room temperature and incubated for 2 h at room temperature or overnight at 4°C with primary antibodies against GFP (Roche), androgen receptor (Invitrogen) ...
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bioRxiv - Genetics 2023Quote: ... Final libraries were quantified by qPCR using the KAPA Library Quantification Kit (Complete kit; Universal) (Roche) and sequenced on a MiSeq sequencer using a 300-cycle v2 kit (Illumina).
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bioRxiv - Genetics 2023Quote: ... A-tailed (using KAPA High Throughput Library Preparation Kit; Roche), ligated with a hairpin adapter (NEB) ...
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bioRxiv - Genetics 2023Quote: ... and subject to library construction using the KAPA HyperPrep Kit (Roche) with Illumina-competent adapters (Supplementary Table 5 ...
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bioRxiv - Genetics 2023Quote: ... and amplified by PCR using KAPA HiFi HotStart ReadyMix (Roche). Completed libraries were quantified by qPCR using KAPA Library Quantification kit (Complete kit ...
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bioRxiv - Cell Biology 2023Quote: ... or 50 μg/ml DNase (04536282001, Roche Diagnostics, Mannheim, Germany) were added to cells ...
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bioRxiv - Cell Biology 2023Quote: ... 1X cOmplete Protease Inhibitor Cocktail (11836170001, Roche), 10 mM N-Ethylmaleimide ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% sodium deoxycholate) with protease inhibitors (1mM PMSF, 1mM Benzamidine, 1mg/ml Bacitracin, one Roche Tablet (catalog # 11836170001) in 10ml ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing was performed using the SeqCap EZ Human Exome Library (Roche) and a NovaSeq 6000 sequencer (Illumina) ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR gene expression analysis was conducted using a LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland) with SYBR Green I Master Mix reagent (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... Bacterial pellets were lysed in 10 ml B-PER Bacterial Protein Extraction Reagent (Pierce, Rockford, ILL) with Complete Protease Inhibitor Cocktail Tablets (Roche, Indianapolis, IN). Extracts were cleared by centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... XtremeGene 9 (Roche Diagnostics) was used.
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bioRxiv - Cell Biology 2023Quote: ... with SYBR Green I Master Mix reagent (Roche) and the primers listed in Table 1 ...
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bioRxiv - Cell Biology 2023Quote: ... and a complete protease inhibitor cocktail (Roche). The resulting supernatants ...
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bioRxiv - Cell Biology 2023Quote: ... cytospins and membranes were permeabilized by 0.1% Triton X-100 (Roche, SIG10789704001) for 15 min and blocked with 2% BSA in PBS for 1 hour at room temperature (RT) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Quantitative PCR was performed using FastStart DNA SYBR Green on LightCycler 1.5 (Roche Diagnostics), with primer set of 5’-AGACAGTGGTTGCCTACGGG-3’ and 5’-ATGCGAAGTGTCCCATGAGC-3’ ...
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bioRxiv - Genetics 2023Quote: ... resuspended in 1 bead volume of IP buffer containing 2X complete protease inhibitors (Roche) and sonicated on ice at an amplitude of 30% for 2.5 min (15’’ ON/15’’ OFF pulses ...
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bioRxiv - Genomics 2023Quote: ... The cDNA was amplified by combining 25 μL of the silane purified product with PCR master mix (25 μL 2X Kapa HotStart Mix (Kapa Biosystems #KK2602), 2 μL of 5 μM SMART PCR primer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries for target enrichment of ∼3 Mb of Lupinus angustifolius genomic material were produced using the Roche Sequencing Solutions’ ‘SeqCap EZ – HyperPlus’ kit (Roche Sequencing Solutions, Pleasanton, CA) with 200 ng/L of input DNA.
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bioRxiv - Evolutionary Biology 2023Quote: ... supplemented with protease inhibitor (Roche), 1 μL/mL Ready-Lyse Lysozyme Solution (Lucigen ...
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bioRxiv - Genomics 2023Quote: Fresh pinch biopsies were washed twice in DPBS before being coarsely chopped with a scalpel and transferred into 0.5 mL DPBS solution containing 0.1 WU ml−1 Liberase TH (Roche, 5401151001) and 0.5 U ml−1 RQ1 DNase I (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... gfp (Hayashi et al., 2015) and dusp6 (Gomez et al., 2005) were prepared according to the protocol of the manufacturer (Roche). To prepare serial cryosections ...
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bioRxiv - Developmental Biology 2023Quote: ... and NBT/BCIP substrate (Roche) were used to detect the probe ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized with Transcriptor Reverse Transcriptase (Roche, cat# 3531287001), using an oligo(dT)18sequence ...
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bioRxiv - Developmental Biology 2023Quote: ... Anti[DIG alkaline phosphatase-conjugated antibody (Roche; 1/2000 diluted) and NBT/BCIP substrate (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... 100 μl of the lysis buffer was added to each 6-well plate along with 1×Protease Inhibitor Cocktail (PIC, Roche cOmplete). The cells were incubated in a rocker at 4°C for 1hr ...
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bioRxiv - Developmental Biology 2023Quote: ... and 1x Protease Inhibitor cocktail (PIC) (Roche, SKU11697498001). The total protein concentration in the cell lysate was quantified by using BCA protein Assay kit (Thermo Scientific™ ...
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bioRxiv - Developmental Biology 2023Quote: ... in the PCR instrument Light Cycler 480 (Roche) or BioRad ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were in situ hybridized according to (Harland and Biology, 1991), bleached (Sive et al., 2000) after staining with BM Purple (Roche #11442074001) and imaged ...
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bioRxiv - Developmental Biology 2023Quote: ... and the Digoxigenin labeling kit (Roche). Both anti-sense and sense probes were made for each gene ...
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bioRxiv - Developmental Biology 2023Quote: ... RNAse inhibitor and dig-labeled rNTPs (Roche, #3359247910 and 11277057001). Embryos were in situ hybridized according to (Harland and Biology ...
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bioRxiv - Developmental Biology 2023Quote: ... Riboprobes were synthesized using the DIG-labelling kit (Sp6/T7) from Roche. Animals were mounted in 70% glycerol before imaging.
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bioRxiv - Developmental Biology 2023Quote: Double-stranded RNA (dsRNA) was synthesized by in vitro transcription (Sp6/T7 from Roche) as previously described (Sánchez-Alvarado and Newmark ...
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bioRxiv - Developmental Biology 2023Quote: ... 1μg linearized plasmid was used for In Vitro transcription to make DIG labelled RNA probe (DIG RNA Labeling Mix-11277073910 Roche). Whole mount RNA in situ hybridization was performed by following the standard zebrafish protocol (http://zfin.org/ZFIN/Methods/ThisseProtocol).
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bioRxiv - Developmental Biology 2023Quote: ... The probes were detected using anti-DIG POD-conjugated antibody (Roche, Cat# 11207733910) and anti-DNP HRP-conjugated antibody (Perkin Elmer ...
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bioRxiv - Developmental Biology 2023Quote: ... Detection was performed using an anti-digoxigenin AP-conjugate antibody (Roche, Cat# 11093274910) followed by an NBT/BCIP reaction (Roche) ...
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... Embryos were stained with 20μl/mL NBT/BCIP substrate (Roche 11681451001) in the dark for 5-10 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... and 1x cOmplete Protease Inhibitor (Roche #11836153001)) with a 1 cc syringe and 25×5/8 needle ...
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bioRxiv - Developmental Biology 2023Quote: ... BCIP/NBT (Roche) was used as a color developing agent ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were incubated with 1:5,000 dilution of anti-digoxigenin-AP conjugated antibody (Roche) overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... for nucleic acid hybridization and detection (BBR, Roche, 11096176001) in MAB buffer at RT for 90 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1% Tween20 in H2O) and color reaction was developed with BM purple (Roche, 1442074) at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... and DIG RNA Labelling Mix (Roche) according to the manufacturers’ protocols and (Genikhovich and Technau ...
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bioRxiv - Developmental Biology 2023Quote: ... and 1 μl of Alkaline Phosphatase-linked α-Digoxigenin antibody (Roche, 11093274910) per sample ...
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bioRxiv - Developmental Biology 2023Quote: ... and 3% Blocking Reagent (Roche) to the hybridization mix during overnight blocking and hybridization of the probe ...
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bioRxiv - Developmental Biology 2023Quote: ... Pieces of tissue were incubated in 500 ml of NBT/BCIP solution (4.5 μl/ml NBT, 3.5 μl/ml BCIP in Alkaline Phosphatase buffer) (Roche 11383213001 and 11383221001) in the dark from some minutes to several hours ...
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bioRxiv - Developmental Biology 2023Quote: ... 2018) were combined with a transgenic construct (9 µg) and co-transfected using X-tremeGene HP (Roche Applied Science) at a 1:3 ratio of DNA to the transfection reagent ...