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Citations for Roche :
9701 - 9750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... containing protease and phosphatase inhibitor cocktails (Roche, Indianapolis, Indiana). The mouse kidneys were extracted using the same lysis buffer with the protease and phosphatase inhibitors and homogenized by sonication ...
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bioRxiv - Cell Biology 2023Quote: Library preparation was performed with 500 ng of total RNA where the ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems), fragmented in reverse transcriptase buffer and heated to 94°C for 8 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... productively edited cells were selected with 0.3 mg mL-1 hygromycin B (Roche, 10843555001). Homozygously edited clones were identified using the same genotyping strategy as described above and cells which efficiently depleted WAPL were identified using confocal microscopy and Western blotting ...
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bioRxiv - Cell Biology 2023Quote: ... and hygromycin B (0.3 mg mL-1, Roche, 10843555001). All cell lines were cultured in a humidified incubator at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorescein (Ver16.0, Roche, Mannheim, Germany) per the instructions.
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bioRxiv - Cell Biology 2023Quote: ... containing 0.5% fatty acid-free BSA (Roche), 10 mg ml-1 Collagenase D (Roche) ...
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bioRxiv - Cell Biology 2023Quote: U2OS cells were transfected with the indicated plasmids with Fugene 6 (Roche) according to manufacturer’s instructions using a 3:1 Fugene/plasmid ratio ...
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bioRxiv - Cell Biology 2023Quote: ... with virus packaging and envelope expressing plasmids using Fugene-6 (Roche). Medium was replaced with DMEM with 10% FCS the next day and supernatant was harvested 3 days post-transfection ...
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bioRxiv - Cell Biology 2023Quote: ... 10 mg ml-1 Collagenase D (Roche), 3 U ml-1 Dispase II (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... 50 mM NaF, 1 mM EDTA, 1 mM EGTA, and 1% Triton X-100, supplemented with Complete EDTA-free protease inhibitor [Roche]). Cell samples were rotated for 10 min at 4 °C to achieve lysis ...
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bioRxiv - Cell Biology 2023Quote: ... 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 2x Complete EDTA-free protease inhibitor [Roche]), and insoluble material and lipid were cleared by centrifugation at 16,000 g for 15 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Basal respiration was measured in DMEM containing 2% fatty-acid-free BSA (Roche). Cells were treated with 100 μM isoproterenol to stimulate respiration ...
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bioRxiv - Cell Biology 2023Quote: ... 3 U ml-1 Dispase II (Roche), and 5 mM CaCl2 for 45 min at 37 °C with shaking ...
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bioRxiv - Cell Biology 2023Quote: ... The additional mutation in construct M1 (P234G) was generated by PCR (Pwo Master; Roche) using primers ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with cOmplete protease inhibitor (1×, Roche, 11873580001), 1 mM PMSF ...
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bioRxiv - Cell Biology 2023Quote: ... using the LightCycler 480 SYBR Green I Master mix (Roche, Cat. # 04887352001), according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... and 0.1% SDS) supplemented with protease inhibitors (Roche, Cat. # 11836170001). SDS-PAGE gels were used for protein separation followed by transfer onto PVDF membrane (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... anti-GFP (1:1000, 11814460001, Roche). The membrane was washed 3 times with 5% milk TBST and incubated with HRP-conjugated secondary antibodies (1:5000 ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were transfected using X-tremeGENE9 (Roche) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with complete EDTA-free protease inhibitor tablet (Roche, Cat. # 11836170001)] ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR (qPCR) was performed on LightCycler® 480 Instrument II (Roche) using the LightCycler 480 SYBR Green I Master mix (Roche ...
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bioRxiv - Cell Biology 2023Quote: RNA was obtained from epithelioids 1 week after medium change to mFAD or from mouse esophageal epithelium peeled and incubated with Dispase I (Roche catalog no. 04942086001) diluted at 1 mg/ml in PBS for 15 min ...
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bioRxiv - Biophysics 2023Quote: ... supplemented with protease inhibitors (Roche) according to manufactures’ protocol ...
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bioRxiv - Biophysics 2023Quote: ... 300 mM NaCl with a protease inhibitor cocktail tablet (Roche). Resuspended pellets were stored at −80 °C.
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bioRxiv - Biophysics 2023Quote: ... and harvested cell pellets resuspended in 250 μl lysis buffer containing a complete protease inhibitor cocktail (Roche). After centrifugation (15,000 × g for 5 min at 4°C) ...
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bioRxiv - Biophysics 2023Quote: ... and 1x complete protease inhibitor cocktail (#11836170001, Roche)] and lysed by sonication (total 2min ...
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bioRxiv - Biophysics 2023Quote: ... and 1X EDTA-free complete protease inhibitor cocktail (Roche). The cells were lysed with an Emusliflex C3 Homogenizer and the cell lysate was clarified by centrifugation (Sorvall RC-5B Superspeed Centrifuge ...
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bioRxiv - Cell Biology 2023Quote: Lipofection: Plasmid was lipofected into cells using X-tremeGENE 9 DNA Transfection Reagent (Roche; Basel, Switzerland). The plasmid:transfection reagent ratio (w:v ...
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bioRxiv - Cell Biology 2023Quote: Adult rat ventricular cardiomyocytes were freshly isolated by Langendorff perfusion with Liberase TM (0.225 mg/mL, Roche) in modified Krebs-Henseleit buffer (135mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... washed with 100 ul/sample of Wash Buffer (20mM HEPES pH7.5, 150mM NaCl, 0.5mM Spermidine, 1x Roche cOmplete EDTA-free Protease Inhibitor (1187358001) ...
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bioRxiv - Cancer Biology 2023Quote: Whole-cell protein lysates were harvested in lysis buffer (RIPA buffer supplemented with protease inhibitor (Roche), sodium vanadate and sodium molybdate ...
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bioRxiv - Cancer Biology 2023Quote: ... Consecutive sections were stained with hematoxylin and eosin (H&E) or subjected to immunohistochemistry using automated immunostaining platforms (Ventana Discovery XT, Roche or Autostainer Plus Link 48). Antigen retrieval was first performed with high or low pH buffer depending on the primary antibody (CC1m ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was extracted from frozen muscle samples using TriPure Isolation Reagent (Roche Diagnostics, Basel, Switzerland), as described by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... erythrocytes were lysed in 2 mL red blood cell lysis buffer (Roche, 11814389001) for 5 min at room temperature before the addition of 10mL AdDF+++ and centrifugation at 400 rcf ...
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MIIP downregulation promotes colorectal cancer progression via inducing adjacent adipocytes browningbioRxiv - Cancer Biology 2023Quote: ... and 1.5 mg/mL collagenase I (Roche). Digested tissue was filtered through a 200-μm cell strainer and centrifuged at 300g for 5 min to pellet the ADSCs ...
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bioRxiv - Cancer Biology 2023Quote: ... and 20 mM Tris pH 8) supplemented with 1 mg/mL of protease inhibitors (Roche). Cell lysates were sonicated for 10 cycles with Bioruptor Pico (Diagenode ...
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bioRxiv - Cancer Biology 2023Quote: ... and 140 mM NaCl) containing phosphatase and EDTA-free Protease Inhibitor Cocktail (Roche). Cell lysates incubated on ice for 30 min and sample were centrifuged at 12,000 rpm for 15 min at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... Cells were pelleted and resuspended in 50 mM Tris (pH 7) supplemented with 500 μg/mL lysozyme and 1x cOmplete EDTA-free protease inhibitor cocktail (Roche, 11873580001). The pellet was incubated at 4 ºC while rotating end over end for 45 min.
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bioRxiv - Biophysics 2023Quote: ... 10 mM β-mercaptoethanol) plus 1× cOmplete™protease inhibitor cocktail (Roche). The lysate was centrifuged at 22 ...
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bioRxiv - Cell Biology 2023Quote: ... Livers were collected on ice and incubated in digestion buffer (0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum) at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues were incubated in digestion buffer (0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum) for 45 minutes at 37°C while agitating at 750 rpm on an Eppendorf ThermoMixer ...
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bioRxiv - Cell Biology 2023Quote: ... 1x Protease inhibitor (11 873 580 001, Roche)) ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used in the study: rat anti-HA (Roche, Basel, Switzerland), rabbit anti-HA (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... liberase TL Research Grade low Thermolysin (Roche), DNase I ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting cDNA was analysed by qRT-PCR on a LightCycler 480 using SYBR Green 1 Master Mix (Roche). Relative fold change in expression was determined by the ddCt method ...
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bioRxiv - Cell Biology 2023Quote: cDNA synthesis was performed using the Transcriptor Reverse Transcriptase (3531317001, Roche) with an oligo-dT anchor primer (5’-GACCACGCGTATCGATGTCGAC-TTTTTTTTTTTTTTTTV-3’) ...
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bioRxiv - Cell Biology 2023Quote: ... and cOmplete EDTA-free protease inhibitor cocktail (11836170001, Roche)] and incubated 15 minutes on ice ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 1 µg/mL plasmid DNA using Fugene HD transfection reagent (Roche Diagnostics, Indianapolis, IN).
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bioRxiv - Cell Biology 2023Quote: ... anti-GFP (Roche, Indianapolis, IN), or anti-GFP (VUIC9H4 ...
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bioRxiv - Cell Biology 2023Quote: ... UK). Rat anti-HA mAb (3F10) and rabbit anti-GFP pAb (code No. 598) were from Roche (Basel, Switzerland) and Medical and Biological Laboratories (Aichi ...