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Citations for Roche :
8901 - 8950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Complete protease inhibitors (Roche)) ...
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bioRxiv - Genomics 2023Quote: ... on a 384-well LightCycler 480 Real-Time PCR System (Roche) and threshold cycle (Ct ...
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bioRxiv - Genomics 2023Quote: Real-time quantitative reverse transcription PCR (qRT-PCR) was performed using KAPA SYBR FAST (Kapa Biosystems KK4610) on a 384-well LightCycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... Binding reactions were performed in buffer A containing 2x protease inhibitor (Roche) for 1 hour at 4 °C ...
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bioRxiv - Genomics 2023Quote: ... which was performed with the KAPA SYBR FAST (Kapa Biosystems KK4610) on a LightCycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Genomics 2023Quote: ... on a LightCycler 480 Real-Time PCR System (Roche) using either 96-well or 384-well qPCR plates as previously described (Brosh et al ...
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bioRxiv - Genomics 2023Quote: ... Using a P7-specific primer (Extended Data Table S4) UMI’s were added to cDNA (in 5 reactions) with Kapa 2x HiFi HotStart ReadyMix (Kapa Biosystems). Reactions were pooled and purified ...
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bioRxiv - Genomics 2023Quote: ... GenBank accession number Z69354.1) and BlSat1 (Hong Ha 2018) were labelled by PCR in the presence of biotin-16-dUTP (Roche Diagnostics) detected by streptavidin-Cy3 (Sigma–Aldrich ...
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bioRxiv - Genomics 2023Quote: ... and cells were washed and scraped into ice cold PBS containing Complete EDTA-free Protease Inhibitor Cocktail (Roche). Cells were pelleted in aliquots of 3.6*107 cells ...
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bioRxiv - Immunology 2023Quote: ... incubated with 50 μl of anti-digoxigenin POD fragments (Roche, 11 633 716 001) diluted 1:5000 in 0.3% BSA PBS for 1 hour at RT ...
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bioRxiv - Immunology 2023Quote: This assay was performed using in situ cell death detection kit (Roche) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... following by the secondary antibody incubation and chromogenic detection with DISCOVERY Purple and Yellow kits (Ventana-Roche Diagnostics, #760-229 and # 760-239). These chromogenic dyes are covalently deposited and have unique spectra (83 ...
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bioRxiv - Immunology 2023Quote: K2 cells or human neutrophils were lysed with 150-200 µl of lysis buffer (supp. Table 2) supplemented with 1X complete inhibitor (Roche) and incubated for 10 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: Total hemolymph was harvested by bleeding ten L3 wandering larvae in 100 μL of Phosphate Buffer Saline (PBS) supplemented with Complete Inhibitor (Roche, diluted 1/50), PMSF (1 mM ...
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bioRxiv - Immunology 2023Quote: ... cytotoxicity was determined based on the release of lactate dehydrogenase (LDH) into the co-culture supernatant measured by Cytotoxicity Detection Kit (Roche) and using a Synergy 2 Multi-Mode Microplate Reader (Biotek ...
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bioRxiv - Immunology 2023Quote: ... or performed by the LightCycler480 System (Roche) using the LightCycler480 Probes Master (Roche ...
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bioRxiv - Immunology 2023Quote: ... Universal ProbeLibrary Probes #22 (Roche).
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bioRxiv - Immunology 2023Quote: ... and the Universal ProbeLibrary Probes (Roche). Transcripts were normalized to the mHprt/hHPRT transcript ...
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bioRxiv - Immunology 2023Quote: ... and 0.1 mg/mL DNase I (Roche); the proteins of the parietal-cell cytosol and membrane/organelle fractions were extracted with the ProteoExtract Subcellular Proteome Extraction Kit (Millipore ...
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bioRxiv - Genomics 2023Quote: ... RNA libraries were prepared according to the mRNA HyperPrep kit protocol (Roche, Basel, Switzerland). All samples were sequenced in the MSU RTSF Genomics core with paired-end 150 bp reads on an HiSeq 6000 system (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... primer efficiency was first calculated by amplifying cDNA template with KAPA 2× SYBR Master mix (Roche catalog 07959362001) and quantifying the SYBR intensity using a Roche LightCycler 480 at three different concentrations of cDNA 10-fold apart (1/2 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-HA (rat, 11867423001; Roche), anti-YIPF4 (rabbit ...
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bioRxiv - Cell Biology 2023Quote: ... Standards with known NADH concentrations (10107735001, Roche, Sigma-Aldrich), ranging between 0 and 0.75 µmol/ml ...
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bioRxiv - Cell Biology 2023Quote: Cells from a well of 12-well plate were lysed on ice in 80 µL RIPA buffer (Thermo) supplemented with protease inhibitor cocktail (Roche) and Benzonase (Novagen ...
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bioRxiv - Genomics 2023Quote: ... 0.3 mM each dNTP (Roche #KK1017), 2.0 μM each P5 and P7 PCR primer ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was conducted using a Roche LightCycler 480 system (Roche) with three biological replicates ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were further incubated for 48 h and subsequently subjected to Gentamycin (G418) selection (1 μg/ml, Roche, G418-RO). After 4 days cells were sorted on a fluorescence-activated cell sorter (FACS ...
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bioRxiv - Microbiology 2023Quote: ... monoclonal rat anti-HA antibody (Roche, Basel, Switzerland) and mouse anti-GFP antibody (Roche ...
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bioRxiv - Genomics 2023Quote: ... containing Complete Protease Inhibitor (Roche) and PhosSTOP phosphatase inhibitor (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: Total cell lysates were generated from E10.5 embryos using CHAPS buffer (10 mM HEPES, pH 7.4, 150 mM NaCl, 1% CHAPS plus complete protease inhibitor cocktail (Roche, #11697498001)) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell viability was assessed by MTT assay (Roche, #11465007001) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... 0.1% sodium dodecylsulfate (w/v)) supplemented with 1x protease inhibitor cocktail (Roche). Lysates were clarified by centrifugation at 20,000 x G for 10 minutes ...
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bioRxiv - Physiology 2023Quote: ... or 50 Tris-HCl pH 7.5, 1 phenylmethylsulfonyl fluoride, 3% SDS (RyR, SERCA, CaMKIIδ-C273S) and supplemented with cOmplete protease inhibitor (Roche). Lysates were then incubated on ice for 15 min and centrifuged for 15 min at 15000 rcf at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... and lysed immediately in ice-cold lysis buffer (20 mM Tris. HCl, pH 8.5, 8M urea, phosphatase inhibitors (PhosSTOP, Roche), 2 mM dithiothreitol (DTT ...
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bioRxiv - Immunology 2023Quote: ... 12.5 µl of KAPA HiFi HotStart Ready Mix (Roche), 0.03 µl of 100 µM 5UO and 3U primers ...
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bioRxiv - Immunology 2023Quote: ... They were incubated in digestion buffer (1X PBS Ca+Mg+ containing 0.1mg/ml DNase I, Roche 05401127001 ...
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bioRxiv - Immunology 2023Quote: ... RT-qPCR was performed in duplicate for each sample using the LightCycler 480 Real-Time PCR System (F. Hoffmann-La Roche, Ltd.) with the following conditions ...
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bioRxiv - Microbiology 2023Quote: ... followed by overnight in-solution trypsin (Roche, Basel, Switzerland) digestion (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... containing 0.3% BSA (fraction V; Roche), 10 mM HEPES (Corning) ...
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bioRxiv - Microbiology 2023Quote: ... destained and digested with Asp-N (Roche, 20 ng/μL) overnight at 37 °C ...
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bioRxiv - Biophysics 2023Quote: ... 5 mM β-ME) supplied with Complete EDTA-free Protease Inhibitors (Roche) per manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: Testis protein extraction was performed in RIPA buffer with a cocktail of protease inhibitors (Roche cat. 11836170001), which was freshly added before beginning protein extraction ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated in an anti-digoxigenin antibody (alkaline-phosphatase-coupled anti-digoxigenin; RRID:AB_514497, diluted 1:3,500; Roche Diagnostics; Table 2) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... sections were blocked in a saturation solution containing 2% Blocking Reagent (Roche Diagnostics) and 20% sheep serum in MABT for 4h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The hybridization signal was revealed with NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl phosphate) (both from Roche Diagnostics) mixed in B2 in a proportion of 0.45 µl of NBT and 4.5 µl of BCIP in 5 ml of B2 ...
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bioRxiv - Neuroscience 2023Quote: Linearized DNAs were used as templates to synthesize in vitro antisense RNA following the protocol of the RNA polymerase protocol (Roche Diagnostic). The mix containing the DNA ...
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bioRxiv - Microbiology 2023Quote: ... coli cell pellets was carried out by resuspending pellets in 50 mL of lysis buffer (50 mM NaPO4, 300 mM NaCl, 20 mM imidazole, 10 mM 2-mercapoethanol, protease inhibitor (Roche), DNAse (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... using the FastStar Essential DNA Green Master (Roche) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Brain cell isolation was performed by enzymatic digestion with Liberase DH (Roche Diagnostics) and Dispase (Worthington) ...
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bioRxiv - Molecular Biology 2023Quote: ... We used a Quikchange site-directed mutagenesis protocol with the KAPA HiFi DNA polymerase (Kapa Biosystems, cat# KK2101) to modify the Fmnl3 minigene plasmid ...