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Citations for Roche :
9101 - 9150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Expression of mRNA and pre-mRNA was then assayed by semi-quantitative reverse-transcription PCR (qRT-PCR) on a Roche LightCycler480 using SYBR Green I (Roche Diagnostics). Raw fluorescence data were analysed using LinRegPCR to perform background subtraction ...
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bioRxiv - Pathology 2023Quote: ... and quantified using the KAPA Library quantification kit (Roche).
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bioRxiv - Neuroscience 2023Quote: ... with DIG-11-UTP (Roche Diagnostics). The DNP-labeled RNA probes for GFP were derived from the fragment corresponding to nucleotides 1,256–1,983 in pIRES2-EGFP (Takara Bio) ...
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bioRxiv - Neuroscience 2023Quote: ... pH 8.0, incubated in a hybridization buffer [(50% formamide, 5× SSPE, 0.1% SDS, and 1 mg/ml Yeast tRNA (Roche Diagnostics)] containing the Avidin solution (Vector Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... 4% BlockAce (DS Pharma Biomedical) and 0.5× Blocking reagent (Roche Diagnostics)] for 1 hr at RT ...
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bioRxiv - Neuroscience 2023Quote: Mouse brains at 5W were isolated and homogenized in homogenization buffer [(0.32 M sucrose, 10 mM HEPES, 2 mM EDTA and 1× complete protease inhibitor cocktail (Roche Diagnostics), pH 7.4)] ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-GFP antibodies (1:500; 11814460001, Roche Diagnostics), anti-Nav1.1 antibodies (1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... The probes were visualized using the NBT/BCIP kit (Roche Diagnostics), VECTASTAIN Elite ABC kit (Vector laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with the alkaline phosphatase-conjugated sheep anti-DIG (1:500; 11093274910, Roche Diagnostics) and biotinylated rabbit anti-DNP (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... and one tablet each of PhoStop and Complete Mini (04906845001 and 04693124001, Roche). Insoluble materials were removed by centrifugation at 300 × g for 20 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and PhosSTOP (#4906837001, Roche). 20 μg of total proteins were resolved on 10% polyacrylamide gels (except for 6% gels used for PERK detection ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 mM Tris-HCl) supplemented with cOmplete™ Mini Protease Inhibitor Cocktail (#11836170001, Roche, Basel, Switzerland) and PhosSTOP (#4906837001 ...
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bioRxiv - Cancer Biology 2023Quote: ... using GAPDH as an endogenous control on LightCycler 96 PCR system (Roche) using Faststart Essential DNA master mix (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... Blocking was performed with 5 % Bovine serum albumin (Boehringer Mannheim, now Roche) in 1x PBST (1x PBS with 0.05 % Tween-20 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 % blocking reagent (Roche), 10 mM Tris-HCl (pH 7.2)) ...
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bioRxiv - Cell Biology 2023Quote: ... and lysed in lysis buffer (50mM Tris, pH 7.4, 500mM NaCl, 0.4% SDS, 2% Triton-X-100, 1mM DTT, 1x complete protease inhibitor [Roche]). Lysates were sonicated twice for 1 minute at 30% duty cycle at an output level of 4 ...
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bioRxiv - Cell Biology 2023Quote: Mouse ventricular myocytes were freshly isolated by Langendorff perfusion with Liberase TM (0.225 mg/mL, Roche) in Krebs-Henseleit buffer (135mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... The reactions were run in Optical 96-well Reaction Plates using LightCycler 96 Instrument (Roche Molecular Systems, Switzerland) and results were analysed using LightCycler 96 Software version 1.1.0.1320 (Roche Molecular Systems ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR (qPCR) was performed following the manufacturer’s instruction using FastStart Essential DNA Green Master (Roche Molecular Systems, Switzerland). Target cDNAs were amplified using gene specific primer pairs (45S_FW ...
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bioRxiv - Cell Biology 2023Quote: ... The microsome fraction was solubilized in 1% Triton X-100 in PBS (10 mg microsome per ml) with protease inhibitor cocktail (complete mini, Roche) by rocking at 4°C for 60 min ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant was incubated with 5% v/v pre-immune rabbit sera and 20 μl of protein G-beads (Roche) for 1 h on a rotator at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... along with a panel of 3 reference genes (PUM1, RPL13A, ACTB) was performed using TaqMan qPCR chemistry on the Light Cycler 480 II (Roche Diagnostics). According to previously established methods 7 ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... minced finely and digested in pre-warmed PBS (with calcium and magnesium) with 2 mg/ml of collagenase A (Roche, Basel, Switzerland) for 1h on a rotating wheel at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... based chromogenic IHC on tissue samples (FFPE or fresh frozen) was performed on an automated Ventana Discovery ULTRA system (Roche). Fresh frozen tissues were air dried at room temperature for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: Whole slides were scanned at x40 resolution (0.25 um per pixel) on a Ventana DP200 slide scanner (Roche) for chromogenic slides ...
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bioRxiv - Cancer Biology 2023Quote: ... pH 8.0) supplemented with cOmplete ULTRA protease inhibitor cocktail (Roche, 4693124001) and sonicated using a Bioruptor (Diagenode ...
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bioRxiv - Cancer Biology 2023Quote: ... containing a protease inhibitor cocktail (Roche) and benzonase (Thermofisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... The runs were carried out in a LightCycler®480II (Roche, Germany) equipment using the standard program SYBR Green Probe II and controls [25] ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR reactions were set up in 20 µL with 300 nM of oligonucleotides (Table S1) and LightCycler® 480 SYBR Green I Master 2x (Roche, Germany) using three technical replicates per sample ...
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bioRxiv - Cancer Biology 2023Quote: ... following the instructions of the manufacturer of Transcriptor First Strand cDNA Synthesis Kit (Roche, Germany). qPCR reactions were set up in 20 µL with 300 nM of oligonucleotides (Table S1 ...
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bioRxiv - Cancer Biology 2023Quote: ... with random hexamer primers (Roche) to produce cDNA from 2 micrograms total RNA ...
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bioRxiv - Cancer Biology 2023Quote: ... The qPCR was run on a LightCycler® 480 II (Roche).
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bioRxiv - Cancer Biology 2023Quote: For qPCR the LightCycler® SYBR Green system (Roche, 04707516001) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... 1mM NaF) supplemented with Complete Protease Inhibitor (Roche), Phosphatase Inhibitor Cocktails II and III (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... and protease inhibitor cocktail tablets (11836153001, Roche, Basel, Swiss). Protein concentration was measured by Protein Assay Dye Reagent Concentrate (cat ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mM Phenylmethylsulfonylfluoride (Roche, Basel, Switzerland), 1% octyl-beta-D glucopyranoside (Sigma-Alrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Bâle, Switzerland), and obtained by centrifugation at 13,000 × g for 20 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... paraffin-embedded sections on a Ventana Discovery Ultra autostainer (Roche Diagnostics). Following dewaxing and rehydration on board ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by Chromomap DAB IHC detection kit (#5266645001, Roche Diagnostics), counterstaining with Mayer’s hematoxylin ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were detected using an anti-rabbit HQ detection system (#7017936001 and 7017812001, Roche Diagnostics) followed by Chromomap DAB IHC detection kit (#5266645001 ...
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bioRxiv - Cancer Biology 2023Quote: ... The cDNAs were labeled with [α32P]-dCTP using the Random Primed DNA Labeling Kit following the protocol provided by Roche. Total RNA was TRizol (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared by the Van Andel Genomics Core from 500 ng of total RNA using the KAPA mRNA Hyperprep kit (v4.17) (Kapa Biosystems, Wilmington, MA USA). RNA was sheared to 300-400 bp ...
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bioRxiv - Cancer Biology 2023Quote: ... complete protease inhibitor cocktail tablets (Roche Applied Science, Basel, Switzerland) were added to the ethanol ...
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bioRxiv - Cancer Biology 2023Quote: ... and Kapa Illumina Library Quantification qPCR assays (Kapa Biosystems). Individually indexed libraries were pooled and 50 bp ...
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bioRxiv - Cancer Biology 2023Quote: ... using Fast Start Universal SYBR Green Master Mix (Roche) with the following primers ...
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bioRxiv - Cancer Biology 2023Quote: ... and PhosSTOP phosphatase inhibitor cocktail (Roche, Cat No. 04906837001). Protein concentrations were measured by Nanodrop (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... with LightCycler® 480 SYBR Green I Master (4887352001, Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... All qPCR reactions were performed on a LightCycler 480 Multiwell Plate 96 (Roche) using the SsoAdvanced Universal SYBR Green Supermix (BioRad, ...
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bioRxiv - Cell Biology 2023Quote: ... with a protease inhibitor cocktail (Roche #11836170001), incubated on ice for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 5mM EDTA and 2% Protease inhibitor (Roche, Complete) boiled for 10 min at 70°C in SDS loading buffer ...