Labshake search
Citations for Roche :
8701 - 8750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The resulting sgRNA was purified using the High Pure PCR Cleanup Microkit (Roche, 498395500). 60 pg sgRNA and 100 pg Cas9 protein (PNA Bio ...
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bioRxiv - Cancer Biology 2023Quote: ... SYBR GreenⅠ Master (Roche, German) was used for qPCR ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1x protease inhibitors (Roche). Western blots were imaged using Image Studio software (LI-COR) ...
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bioRxiv - Cell Biology 2023Quote: ... and protease (Roche) inhibitor cocktails] ...
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bioRxiv - Cell Biology 2023Quote: ... and protease (Roche) inhibitor cocktails] ...
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bioRxiv - Developmental Biology 2023Quote: ... 500 ng of RNA was prepared for sequencing using the KAPA RNA HyperPrep Kit with RiboErase (Kapa Biosystems). Libraries were sequenced on the NextSeq 500 (Illumina) ...
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bioRxiv - Microbiology 2023Quote: ... 1mM TCEP or DTT supplemented with mini complete EDTA-free protease inhibitor (Roche) and 1mM PMSF) ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with protease inhibitors (cOmplete mini, Roche). The tissue was lysed in a Dounce homogenizer with 40 strokes of a plunger ...
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bioRxiv - Molecular Biology 2023Quote: ... they were blocked with 2% bovine serum albumin (BSA, Roche). Samples were incubated overnight at 4°C in the same blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... stranded mRNA-seq libraries were prepared from total RNA (100 ng) using the KAPA Stranded mRNA-Seq kit (Roche) according to the manufacturer’s standard protocol ...
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bioRxiv - Genetics 2023Quote: ... was amplified from the Tad2-containing pSG-thrC-Phspank plasmid using KAPA HiFi HotStart ReadyMix (Roche, cat #KK2601) with the primer pair tad2KIF and tad2KIr (Supplementary table 15) ...
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bioRxiv - Genetics 2023Quote: ... 10 µL 2x KAPA HiFi ReadyMix (Roche), 3 µL nuclease-free water ...
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bioRxiv - Genetics 2023Quote: Libraries were prepared with SeqCap EZ-HyperPlus (Roche Sequencing Solutions). Library size was analyzed with Bioanalyzer High Sensitivity DNA assay (Agilent technologies) ...
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bioRxiv - Genetics 2023Quote: ... with Digoxigenin-11-dUTP alkali-stable (Roche, 11093088910) at 37°C ...
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bioRxiv - Genetics 2023Quote: ... quantitative PCR was performed using the LightCycler® 480 SYBR Green I Master (Roche, 04887352001) following the standard amplification protocol with 45 cycles in a multi-well PCR plate 384 ...
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bioRxiv - Genetics 2023Quote: ... and 5 µL 2x KAPA HiFi ReadyMix (Roche). The following indexing primers were used (X indicates the positions of the 8 bp indices):
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bioRxiv - Genetics 2023Quote: The DNA of each anti-defense candidate was amplified from the source phage genome using KAPA HiFi HotStart ReadyMix (Roche cat # KK2601). Homologs of verified anti-defense genes were synthesized by Genscript Corp ...
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bioRxiv - Genetics 2023Quote: ... Amplification was performed after the bisulfite conversion using Kapa Hifi Uracil+ (Kapa Biosystems, cat# KK282) polymerase using the following cycling conditions ...
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bioRxiv - Genetics 2023Quote: ... Library preparation was performed using KAPA mRNA HyperPrep Kit (ROCHE-08105936001) according to the recommended protocols ...
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bioRxiv - Genetics 2023Quote: ... permeabilization buffer and solubilization buffer were supplemented with phosphatase inhibitor (Roche) and Halt Protease Inhibitor Cocktail (Thermo Scientific) ...
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bioRxiv - Evolutionary Biology 2023Quote: Libraries were produced using the Kapa Stranded mRNA-seq Kit (24RXN) (KK8420 ROCHE-07962193001) with KAPA Unique Dual-Indexed Adapter Plate (KK8726-08861862001 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and qPCR was performed with the Roche COBAS Ampliprep PCR system (Roche, IN, USA). The detection range was from 20 to 1.7◊108 IU/mL.
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bioRxiv - Evolutionary Biology 2023Quote: ... A total of 600 µL of serum or CSF was used for qPCR according to the standard protocol of the COMBAS TaqMan 96 HBV Test V2.0 Kit (Roche, IN, USA), and qPCR was performed with the Roche COBAS Ampliprep PCR system (Roche ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The signal was visualized with BM Purple (Roche).
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bioRxiv - Evolutionary Biology 2023Quote: ... and in vitro transcribed with sp6 or T7 RNA polymerase (Roche, Basel, Switzerland)) according to the insert direction ...
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bioRxiv - Microbiology 2023Quote: ... and ADNaCl with fatty-acid-free BSA (Roche). For modified Sauton’s solid medium ...
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bioRxiv - Molecular Biology 2023Quote: ... KAPA HiFi DNA Polymerase (Kapa Biosystems) and Ligation Sequencing Kit Primer Mix (part of the 1D Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... Splenocytes from Tcrb KO mice were resuspended in 50 µg/ml Mitomycin C (Roche) in PBS and incubated at 37°C for 1h (mixed repeatedly) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10μL total were prepped using the KAPA RNA HyperPrep Kit with RiboErase (Kapa Biosystems; product #KR1351). Sequencing was performed on an Illumina Next-Seq 500 ...
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bioRxiv - Molecular Biology 2023Quote: SNSs prepared as described above were converted to double stranded DNA (dsDNA) via random priming with random hexamer primer phosphate (Roche) and ligation with Taq DNA ligase (NEB)48 ...
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bioRxiv - Molecular Biology 2023Quote: ... washed and resuspended in RIPA buffer containing Phosphatase inhibitor (Roche 4906837001) and protease inhibitor cocktail (Roche 118361530001) ...
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bioRxiv - Molecular Biology 2023Quote: ... and protease inhibitor cocktail (Roche 118361530001). After sonication (2x 4 times Amp60% ...
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bioRxiv - Neuroscience 2023Quote: Real-time PCR analysis was performed with use of LightCycler 96 real-time PCR system (Roche). The PCR parameters were following ...
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bioRxiv - Microbiology 2023Quote: DNA was purified from virus-infected Vero cells stock by a commercially available kit (High Pure Extraction Kit; Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... with 600 μL of the diluted supernatant and 600 μL MagNA Pure 96 External Lysis Buffer (Roche LifeScience, Basel, Switzerland) as input ...
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bioRxiv - Microbiology 2023Quote: ... RNA was re-extracted from original material using the High Pure RNA Isolation Kit (Roche Diagnostics GmbH Mannheim). A multi segment RT-PCR amplification was performed using the Superscript III high-fidelity RT-PCR Kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... RNA/DNA extraction from all material types was performed on the MagNA Pure 96 platform (Roche Diagnostics GmbH, Mannheim, Germany). Phocine Distemper Virus (PDV ...
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bioRxiv - Molecular Biology 2023Quote: ... and 0.1 % SDS) supplemented with protease inhibitors (Roche Applied Science, Manniheim, Germany) and phosphatase inhibitors (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1x cOmplete EDTA-free Proteinase inhibitor cocktail (PIC, Roche).
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bioRxiv - Molecular Biology 2023Quote: ... and 1x cOmplete EDTA-free Proteinase inhibitor cocktail (PIC, Roche)) ...
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bioRxiv - Genomics 2023Quote: ... The PCR amplifications were run on a LC96 thermocycler (Roche, Basel, Switzerland) with the following parameters ...
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bioRxiv - Genomics 2023Quote: ... qPCR was carried out using the LightCycler FastStart DNA Master SYBR Green I kit (Roche), and specific primers selected from the list of available designed primers at Primer Bank (https://pga.mgh.harvard.edu/primerbank).34 As a reference gene ...
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bioRxiv - Genomics 2023Quote: ... ELISA BrdU Colorimetric assay (Roche) was performed according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... KAPA HiFi HotStart PCR Ready Mix (2×) (Roche, KK2601) was used as polymerase for all the PCR amplification steps in this paper ...
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bioRxiv - Genomics 2023Quote: ... 150mM NaCl and 1x cOmplete proteinase inhibitor cocktail EDTA-free (Roche)) and 2 times with FA-500mM NaCl (50mM HEPES pH 7.5 ...
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bioRxiv - Genomics 2023Quote: TP pre-capture libraries were prepared as previously described [15] using the KAPA HyperPlus kit with UDI adapters (Roche). 17.5µL DNA was used as input for fragmentation for eight minutes ...
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bioRxiv - Genomics 2023Quote: ... 75mM NaCl and 1x cOmplete proteinase inhibitor cocktail EDTA-free (Roche)) and 2 times with FA-150mM NaCl (50mM HEPES pH 7.5 ...
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bioRxiv - Genomics 2023Quote: ... 150mM NaCl and 1x cOmplete proteinase inhibitor cocktail EDTA-free (Roche)) ...
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bioRxiv - Genomics 2023Quote: ... resuspended in cold lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 0.1 mM EGTA, Protease Inhibitor (Roche, 04693159001)) and incubated on ice for 10’ to isolate the cell nuclei ...
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bioRxiv - Molecular Biology 2023Quote: ... and 0.1% NP-40) with cOmplete protease-inhibitor cocktail mini tablets (Roche). Triton-X100 was added to a final concentration of 1% and the samples were incubated on ice for 10-20 mins to lyse the cells ...