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Citations for Roche :
9001 - 9050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... MagNA Lyser Green Beads (Roche) was used for the initial lysis step as described in Shirzadi et al ...
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bioRxiv - Immunology 2023Quote: ... together with random oligonucleotides (Roche, #11034731.001) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... complemented with 0.5 M EDTA and a protease inhibitors cocktail (cOmplete Tablets, Roche, Poland), and incubated for a minimum of 10 min at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... and 1× Complete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Plant Biology 2023Quote: ... Starting amounts for transcript levels were calculated based on the titration curve for each target gene using the LightCycler 480 Software (Roche; version 1.5.1.62). Two technical replicates were used and averaged in analyses for each biological replicate ...
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bioRxiv - Plant Biology 2023Quote: ... Oligo dT18 primer and Transcriptor First Strand cDNA synthesis kit (Roche) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... 10 µl of 2X LightCycler 480 SYBR Green I Master mix (Roche, USA) was used along with 1 µl of 5 µM of each primer and 2.5 µl cDNA template in a final reaction volume of 20 µl ...
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bioRxiv - Plant Biology 2023Quote: ... Libraries were constructed using KAPA mRNA Capture Kit (Roche Diagnostics, Tokyo, Japan) and MGIEasy RNA Directional Library Prep Set (MGI ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in non-denaturing buffer at 4°C (10 mM Tris, 500 mM NaCl, MgCl2 1mM, 3U of DNase I, and protease inhibitor cocktail 1 X from Roche, ref 4693116001, pH 7.9) followed by sonication and ultracentrifugation at 13000 rpm for 45 min at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... Each reaction was performed in 25 μL total volume with 12.5 μL KAPA HiFi HotStart ReadyMix (Roche), one μL from 10 μM of each primer ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation with 20 μl of protein G-beads (Roche) for 1 h on a rotator at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were developed in a NBT/BCIP solution (nitroblue tetrazolium chloride/5-bromo-4-chloro-3-indoxyl phosphate; Roche; Basel, CH) for up to 20 min at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 mg/mL BSA (Roche), 60 µg/mL catalase (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... The cDNA was amplified by PCR using KAPA HiFi HotStart ReadyMix (Roche), using M2-2 and SEQ7 (5’-ATATCGTCTCGTATTAGTAGAAACAAGGTAG-3’ ...
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bioRxiv - Neuroscience 2023Quote: ... EDTA-free protease inhibitor cocktail tablet (Roche #4693159001 ...
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bioRxiv - Cell Biology 2023Quote: ... containing protease inhibitor cocktail (Roche, Indianapolis, IN or Sigma Aldrich) and 1 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Molecular Biology 2023Quote: ... Each Ct value was measured using Lightcycler 480 (Roche) and each mean dCt was averaged from triplicate qRT-PCR reactions ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Total protein was extracted with radio immune precipitation assay (RIPA) buffer containing phenylmethylsulphonyl fluoride (PMSF) (Roche, Switzerland) and a protease inhibitor cocktail (Roche ...
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bioRxiv - Immunology 2023Quote: ... and measured using the LightCycler®480 Instrument II (Roche). Relative expression was calculated by LinRegPCR27 and the N0 values were normalized to the geometric mean of reference genes beta-actin ...
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bioRxiv - Immunology 2023Quote: ... antibodies which were detected with SA-POD (Roche). After a final wash ...
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bioRxiv - Immunology 2023Quote: ... RNA from dermal fibroblasts cell pellets were extracted using High Pure RNA Isolation Kit (Roche) using manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... qPCR was performed in 384-well plates using the LightCycler 480 Instrument II (Roche). Target gene expression change was calculated relative to non-targeting controls using the ddCt method.
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bioRxiv - Genomics 2023Quote: ... and 100 μg/ml DNAse I (Roche); red blood cells were lysed (Red Blood Lysis buffer ...
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bioRxiv - Immunology 2023Quote: ... 1x complete protease cocktail inhibitor (Roche) and 5 IU mL−1 benzonaze (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... 1% Triton X-100) and supplemented with protease inhibitors (Roche, cat.no. 11836170001). Cell lysate was incubated for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Real-time PCR was performed using qPCR primer pairs listed in Table S2 and LightCycler480 SYBR Green I Master Mix (Roche) in a LightCycler480 instrument ...
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bioRxiv - Cell Biology 2023Quote: ... qPCR was performed using the LightCycler 480 (Roche) and LightCycler 480 SYBR Green I Master Mix (Roche ...
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bioRxiv - Microbiology 2023Quote: ... 20 µl TUNEL reaction mixture (In situ Cell Death Detection Kit, TMR-red, Roche) was then added to each coverslip and the reaction incubated in the dark for 1 hour at 37°C ...
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bioRxiv - Genomics 2023Quote: ... 12 μL of KAPA_mix [1X KAPA HiFi HotStart buffer Ready Mix (Roche, Cat. No KK2601), 0.5 μM LIDAR_preamp_f ...
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bioRxiv - Genomics 2023Quote: ... and the pellet resuspended in 100 µl of ice-cold RIPA lysis buffer containing 1× protease inhibitor cocktail (Roche), incubated for 30 min on ice ...
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bioRxiv - Genomics 2023Quote: ... 1x complete protease inhibitor and phosphatase inhibitor (Roche). Lysate was sonicated using Active Motif dounce homogenizer ...
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bioRxiv - Genomics 2023Quote: ... We quantified libraries by qPCR using the Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems, cat#KK4824) and the 7900HT Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Genomics 2023Quote: ... we performed amplification using Kapa Hifi Uracil+ (Kapa Biosystems, cat# KK282) polymerase based on the following cycling conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... and libraries were generated using the KAPA Stranded mRNA-seq kit (Roche). RNA-seq libraries were sequenced on the Illumina NovaSeq 6000 and sequencing reads were aligned to hg19 build of the human genome using HISAT2 [24] ...
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bioRxiv - Microbiology 2023Quote: Cell lysates for intracellular chemokine quantification were obtained via repeated freeze-thaw cycles at −80°C of cells suspended in media containing protease inhibitor cocktail (Roche Applied Science) and final centrifugation at 8000g to pellet debris ...
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bioRxiv - Genomics 2023Quote: ... Quantitative PCR was carried out on standards and test samples using the LightCycler 480 Probes Master kit (Roche, 04707494001) with inverted terminal repeat probe and primer sequences indicated in Table S4.
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bioRxiv - Genomics 2023Quote: ... protease inhibitors (1 tablet per 5 mL, Roche 4693159001)) was added to the embryo pellet ...
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bioRxiv - Plant Biology 2023Quote: The 2nd generation DIG Gel Shift EMSA kit (Roche, USA) was used to label the probes with digoxigenin as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Color development was performed in solution containing NBT/BCIP (Roche).
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bioRxiv - Developmental Biology 2023Quote: ... Sections on slides were post-fixed at 4% PFA in 0.1M PB and treated with proteinase K (Roche). Sections were hybridized with digoxigenin (DIG)-labeled probes at 72°C overnight in hybridization solution ...
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bioRxiv - Developmental Biology 2023Quote: ... The optimal numbers of PCR cycles were pre-determined using the KAPA Real-Time Library Amplification Kit (Roche) as described previously (Tanegashima et al ...
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bioRxiv - Bioengineering 2023Quote: ... RNase inhibitor 40U (Roche, Mannheim, Germany), 10×Transcription Buffer 20 µL ...
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bioRxiv - Immunology 2023Quote: ... and equal amounts of protein from lysate were incubated with 5 μg anti-FLAG antibody (Roche) overnight ...
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bioRxiv - Immunology 2023Quote: ... 1x cOmplete protease inhibitor cocktail (+EDTA) (Roche, 11697498001), 250 U/replicate benzonase (Merck ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used in this study and their respective dilutions were mouse monoclonal anti-GFP at 1/1000 (clones 7.1 and 13.1, Roche), mouse monoclonal anti-TY at 1/250 (53) ...
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bioRxiv - Microbiology 2023Quote: ... All analyses were performed using FastStart Universal SYBR Green (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The parasites were then lysed in 300 μL of cold hypotonic buffer (10 mM HEPES, pH 7.5) supplemented with protease inhibitors (Roche), passed through 25G needle (5x ...
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bioRxiv - Microbiology 2023Quote: ... mouse anti-GFP (1:1000, Roche Diagnostics) and rabbit anti-mCherry (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... and qRT-PCR was performed on a Roche 480 Lightcycler with KAPA SYBR FAST (Kapa Biosystems KK4610) after plate loading with an Echo 550 liquid handler ...
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bioRxiv - Genomics 2023Quote: ... Magnesium chloride was added to a final concentration of 5 mM and KAPA HiFi 2x ReadyMix (Roche) was used ...