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Citations for Roche :
8601 - 8650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... sequencing libraries were prepared using KAPA Hyper Prep Kit (KAPA Biosystems). Sequencing of the cDNA libraries was performed by HiSeq2500 (Illumina).
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bioRxiv - Immunology 2023Quote: Cell lysates were prepared in RIPA buffer supplemented with complete protease-inhibitor cocktail (Roche) and mixed in equal amounts with Laemmli buffer (Roth ...
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bioRxiv - Immunology 2023Quote: ... The transcripts for genes of interest were measured by real-time qPCR with a Lightcycler 480 II system (Roche) and a Brilliant III SYBR Green Master mix (Agilent ...
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bioRxiv - Immunology 2023Quote: ... mRNA amplification was analysed by second derivative maximum algorithm (LightCycler 480 Sw 1.5; Roche) and expression of the gene of interest was normalised to a combination of housekeeping genes as specified in each figure.
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bioRxiv - Immunology 2023Quote: ... and protein from whole cell fraction as well as supernatants were extracted with M-PER buffer from Thermo Fisher Scientific (Cat. No. 78501, Waltham, MA, USA) with protease inhibitors from Roche/Sigma-Aldrich (Cat ...
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bioRxiv - Immunology 2023Quote: ... TMR red (Roche). Tissues were washed three times and mounted in Dako Fluorescence Mounting Medium (Agilent).
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bioRxiv - Immunology 2023Quote: ... Kidneys were homogenized using the Miltenyi gentleMACS Dissociator and digested for 20 minutes at 37°C in RPMI with 2 mg/mL collagenase D (Roche) and 0.1 mg/mL DNAse I ...
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bioRxiv - Immunology 2023Quote: ... 2 μg/ml Histon (Roche), 1 μg/ml Sm/RNP (GenWay) ...
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bioRxiv - Immunology 2023Quote: ... Universal ProbeLibrary Probes #39 (Roche). Human mCTLA-4 ...
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bioRxiv - Immunology 2023Quote: ... Universal ProbeLibrary Probes #69 (Roche). Human HPRT1 ...
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bioRxiv - Immunology 2023Quote: ... and 0.1 mg/mL DNase I (Roche; Sigma-Aldrich)] in the Bioshaker (TAITEC ...
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bioRxiv - Immunology 2023Quote: ... with the Complete mini EDTA-free (Roche); the cell lysate was centrifuged for 10 minutes at 18,000 x g at 4°C and the supernatant was collected ...
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bioRxiv - Immunology 2023Quote: ... in antibody buffer (20 mM HEPES pH 7.5; 150 mM NaCl; 0.5 mM Spermidine; 1X Protease inhibitor cocktail (Roche); 0.05% Digitonin ...
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bioRxiv - Immunology 2023Quote: ... Amplified DNA libraries were purified by adding 1.3x volume of KAPA pure SPRI beads (Roche) to each sample and incubated for 10 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... using the LightCycler480 Probes Master (Roche) and the Universal ProbeLibrary Probes (Roche) ...
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bioRxiv - Immunology 2023Quote: ... The DNA samples were treated with 0.5 μg/μl of RNase A for 30 min at 37°C and then with 0.25 μg/μl of Proteinase K (Roche) in the presence of 10mM EDTA ...
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bioRxiv - Immunology 2023Quote: ... Input DNA was obtained following the sample phenol-chloroform purification steps post overnight de-crosslinking of 10 μg of sonicated sample at 65 °C in the presence of 0.2 M NaCl and 10 μg/μl of RNase A and 0.8 μg/μl of Proteinase K (Roche). DNA was quantified with the Qubit dsDNA HS Assay Kit (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... Magnus Fontes (Institut Roche), Gunilla Karlsson Hedestam (Karolinska Institutet) ...
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bioRxiv - Immunology 2023Quote: ... Apoptosis also was detected using a terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick end-labeling (TUNEL) assay with Cell Death Detection Kit (Roche, Germany). Nuclei were stained with 4-6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2023Quote: ... Aortas were manually homogenized and digested for 20 minutes at 37°C in RPMI with 1 mg/mL of collagenase A and collagenase B and 0.1 mg/mL DNAse I (Roche). Kidneys were homogenized using the Miltenyi gentleMACS Dissociator and digested for 20 minutes at 37°C in RPMI with 2 mg/mL collagenase D (Roche ...
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bioRxiv - Immunology 2023Quote: ... Retinas of each model were pooled and digested in 1.5 mg/ml collagenase A and 0.4 mg/ml DNase I (Roche) for 45 min at 37°C with agitation ...
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bioRxiv - Immunology 2023Quote: ... with protease inhibitors (Roche, 11836170001), quantified with Pierce 660 nm Protein Assay Reagent (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... 10 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) using 8 cycles of PCR ...
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bioRxiv - Immunology 2023Quote: ... Serum lipid panel was measured using Cobas b 101 POC system and its lipid disc (both from Roche Diagnostics). For liver cholesterol measurements ...
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bioRxiv - Immunology 2023Quote: ... Washed bladders were incubated for 1 h at 37 °C in 0.34 U/mL of Liberase (Roche) in PBS ...
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bioRxiv - Immunology 2023Quote: ... Technical triplicates were run for each sample using LightCycler® 480 SYBR Green Mix (Roche) and analysed with a LightCycler instrument (Roche).
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bioRxiv - Immunology 2023Quote: ... Antibody detection was further performed using streptavidin–alkaline phosphatase (11089161001, Roche) for 1 hour and then alkaline phosphatase substrate solution containing 4-nitro-phenyl phosphate (N2765-50TAB ...
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bioRxiv - Immunology 2023Quote: ... KAPA SYBR FAST One-Step universal kit (Roche) and LightCycler 96 (Roche ...
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bioRxiv - Immunology 2023Quote: ... The pellets were resuspended with 100 μl of solubilization buffer containing protease inhibitor cocktail (11836153001, Roche) and incubated for 30 min on ice with constant mixing ...
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bioRxiv - Microbiology 2023Quote: ... All qPCR assays were carried out on a LightCycler 480 (Roche Diagnostics) using the LightCycler 480 software version 1.5.1.
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bioRxiv - Microbiology 2023Quote: ... with protease inhibitors (cOmplete, Roche, Basel, Switzerland)) ...
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bioRxiv - Microbiology 2023Quote: ... on the LightCycler 480 System (Roche) with the following primers ...
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bioRxiv - Molecular Biology 2023Quote: ... 1μl 10mM of ddATP/ddTTP/ddCTP/ddGTP (Roche, 12352200) was added at the beginning.
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bioRxiv - Molecular Biology 2023Quote: ... The cells were lysed in 150 µL RIPA buffer with 2x complete protease inhibitor cocktail (Roche) on ice for 10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR was performed in technical triplicates using the 5x HOT FIREPol EvaGreen qPCR Supermix (Solis Biodyne) and the LightCycler 480 II instrument (Roche). For RT-qPCR normalization ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR was performed using the 5x HOT FIREPol EvaGreen qPCR Supermix (Solis Biodyne) and the LightCycler 480 II instrument (Roche) as technical triplicates ...
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bioRxiv - Neuroscience 2023Quote: ... Reactions were developed in a LightCycler® 96 (Roche). Reverse transcription was performed for 30 min at 48 °C ...
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bioRxiv - Microbiology 2023Quote: ... TE Select-D G-25 spin columns (Roche Applied Science) were used to remove unincorporated [μ-32P]dATP ...
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bioRxiv - Microbiology 2023Quote: DNA from all cervical smears was isolated using MagNA Pure (Roche, Bazel, Switzerland). The purified DNA was eluted in 50 μl TE-buffer (28 ...
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bioRxiv - Neuroscience 2023Quote: ... and phosphatase inhibitor cocktail (Roche; 4906837001) and quantified using a Pierce™ BCA protein assay (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and 1× cOmplete EDTA-free protease inhibitor (Roche). Lysis was allowed on ice for 30min ...
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bioRxiv - Neuroscience 2023Quote: ... Pellets were then dissolved in RIPA buffer with 1× Phos-STOP (Roche) and 1× cOmplete EDTA-free protease inhibitor (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... Pellets were resuspended in lysis buffer (20mM Tris-HCL pH 8.0, 300mM NaCl; 4ml/100ml culture) with freshly added protease inhibitors (Roche #11697498001: 1 tablet/50ml) and lysozyme (0.1mg/ml) ...
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bioRxiv - Microbiology 2023Quote: ... and lysed using NP-40 lysis buffer (50 mM Tris [pH 8.0], 150 mM NaCl, 1% NP-40) supplemented with Complete Protease Inhibitor Cocktail (Roche) for 30 minutes on ice ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were centrifuged at 10,000 x g for 15 minutes to pellet and remove insoluble material after which the remaining soluble lysate was incubated with anti-HA sepharose beads (Roche) for 4 hours at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... microvessels obtained in the pellet were combined into a new conical sterile tube and further digested with collagenase-dispase (1 mg/ml, Roche) and DNase I (6.7 μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... were ligated to the individual cDNA products to barcode the samples and Kapa HiFi HotStart Library Amplification kit (kk2612, Roche Sequencing and Life Science) was used for library amplification.
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bioRxiv - Neuroscience 2023Quote: ... pH 7.6 containing 1% sodium deoxycholate with 1X protease/phosphatase inhibitor cocktail (Roche, #05892970001). Samples were then sonicated ...
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bioRxiv - Neuroscience 2023Quote: ... TUNEL assay was performed using the In-Situ Cell Death Detection Kit (TMR Red) (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... protease and phosphatase inhibitor cocktail (Roche)) ...