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Citations for Roche :
8401 - 8450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 1 µg/ml Pepstatin A and Protease inhibitor cocktail (Roche, USA). The first five fractions of the flow-through were collected as 1 ml fractions and the rest were collected as 2 ml fractions ...
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bioRxiv - Neuroscience 2023Quote: ... 1X Protease Inhibitor Cocktail (Roche), 1% Phosphatase Inhibitor Cocktail 2 and 3 (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... Multiplexed library pool QC was based on Agilent Tapestation 4200 HS D1000 and Kapa Library Quantification Kit for Illumina platforms (Kapa BioSystems, Boston, MA) and sequenced at shallow depths on Illumina’s iSeq 100 v2 flow cell for 26×10×10×90 cycles for estimated reads per cell ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.6 μL of ATP (Roche, 11140965001) or Adenosine-5’-O-(3-thiotriphosphate ...
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bioRxiv - Molecular Biology 2023Quote: For nuclear cell lysis 5×10^6 cells per condition were resuspended in 1mL of Lysis Buffer + protease inhibitor (cOmplete, Roche) (10mM Tris-HCl pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mg bovine pancreas RNaseA and EDTA-free Protease Inhibitor Cocktail (Roche). Then the cell suspension was centrifuged at 45,000 g for 1.5 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... or FuGENE (Roche) reagents as described by the manufacturers.
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bioRxiv - Neuroscience 2023Quote: ... Sections of mouse brain were homogenized in 10 volumes (w/v) of TBS buffer with a cOmplete protease inhibitor cocktail (Roche), and centrifuged at 1000,000 × g for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... EDTA-free Protease Inhibitor Cocktail (Roche), 1x Protease Inhibitor Cocktail for plant cell and tissue extracts (Merck) ...
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bioRxiv - Molecular Biology 2023Quote: ... the pellet was resuspended in 200 µL of cold buffer D (50 mM Tris/HCl, pH = 8.0, 10 mM EDTA, 1% SDS 1xPIC (cOmplete™, Protease Inhibitor Cocktail; Roche; 04693116001)) per 15 cm plate ...
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bioRxiv - Neuroscience 2023Quote: ... and complete protease inhibitor cocktail (Roche). Protein concentrations were determined by BCA assay (Pierce ...
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bioRxiv - Neuroscience 2023Quote: ... containing DAPI (0.5 µg/ml; Roche).
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bioRxiv - Neuroscience 2023Quote: ... and rat anti-HA (1:100; Roche, Switzerland) (2 ...
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bioRxiv - Neuroscience 2023Quote: ... All lysis buffers were supplemented with PhosStop™ phosphatase inhibitor cocktail (Roche, 04906837001), protease inhibitor cocktail (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... Leptomeninges were placed in 1mg/mL of Collagenase D (Roche cat. #51657128) dissolved in PBS and incubated at 37C for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... consisting of protease and phosphatase inhibitors (Roche, Mannheim, Germany). Afterwards the Pierce BCA Protein Assay Kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... protease inhibitor cocktail (Roche), 25 units of Pierce Universal Nuclease ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR was performed using KAPA SYBR FAST qPCR (Kapa Biosystems, Wilmington, MA, USA) and 200 nM primers in a LightCycler 96 (Roche Life Science ...
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bioRxiv - Molecular Biology 2023Quote: ... and 200 nM primers in a LightCycler 96 (Roche Life Science, Basel, Switzerland). Cycling conditions were 95 °C for 3 min ...
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bioRxiv - Neuroscience 2023Quote: ... and supplemented with a protease inhibitor (Roche, Basel, Switzerland). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labelling) assay kit (Roche Diagnostics, Mannheim, Germany) was used to label dying cells ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmid DNA containing EEEb1 was labeled with cyanine-5-dUTP by Nick Translation Mix (Roche), while plasmid DNA harboring each of the other nine DNA families (EEEb2–EEEb10 ...
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bioRxiv - Molecular Biology 2023Quote: ... while plasmid DNA harboring each of the other nine DNA families (EEEb2–EEEb10) was labeled with digoxigenin-11-dUTP using DIG-Nick Translation Mix (Roche) or with biotin-16-dUTP (Promo Kine ...
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bioRxiv - Molecular Biology 2023Quote: ... or with biotin-16-dUTP (Promo Kine) using Nick Translation Mix (Roche). The plasmid DNA containing EEEb2 ...
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bioRxiv - Neuroscience 2023Quote: ... 1X protease inhibitors (Roche, cat. #11836153001). Protein extracts containing loading buffer NOVEX Tricine SDS sample buffer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with primary antibodies at 1:200 (rat anti-HA, Roche) in the blocking buffer (2% BSA in PBST ...
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bioRxiv - Microbiology 2023Quote: ... diluted in PBS supplemented with protease inhibitors (Complete, Roche), during 30 minutes on ice and centrifuged at 16100 g at 4°C for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 2% Triton X-100) containing ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail (Roche cOmplete, Sigma Aldrich)] ...
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bioRxiv - Microbiology 2023Quote: ... Cross-linked cells were lysed by sonication in lysis buffer (5 mM EDTA, 1% NP-40 in PBS) supplemented with 2X cOmplete™ inhibitor (Roche). Cell lysates were treated with 2 units of Turbo DNase (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were then prepared from 3 ng DNA with the Kapa HyperPrep Kit (Roche) according to the manufacturer’s standard protocol ...
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bioRxiv - Immunology 2023Quote: ... 12.5 µl KAPA HiFi HotStart ReadyMix (KAPA Biosystems, KK2602). The PCR reaction was performed as follows ...
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bioRxiv - Immunology 2023Quote: ... brains and spinal cords were cut into pieces and digested for 30 min at 37 °C with DNase I (Sigma-Aldrich or Roche) at a concentration of 1 mg/ml and collagenase D (Roche ...
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bioRxiv - Immunology 2023Quote: ... Universal (KAPA Biosystems) and sequenced on an Illumina NextSeq 500 with paired-end 75-base-pair (bp ...
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bioRxiv - Immunology 2023Quote: ... Quantitative real-time PCR (RT-qPCR) was performed using LightCycler® 480 SYBR Green I Master (Roche, Germany) and Muc5a primers (IDT ...
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bioRxiv - Immunology 2023Quote: ... lactate dehydrogenase (LDH) was measured in supernatants collected at 48 hours post transfection using cytotoxicity detection kit PLUS Assay (CYTODET-RO, Roche) according to manufacturer’s instructions and plates were read at 490 nm.
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bioRxiv - Immunology 2023Quote: ... One lobe of lung tissue was collected in an Omni Bead Ruptor tube filled with Tripure Isolation Reagent (catalog number 11667165001; Roche). Tissue was homogenized using an Omni Bead Ruptor 24 instrument (5.15 ms ...
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bioRxiv - Microbiology 2023Quote: ... RNA was reverse transcribed into complementary DNA (cDNA) using Transcriptor reverse transcriptase kit (Roche Diagnostics, UK). qRT-PCR was carried out for Spy0107 ...
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bioRxiv - Microbiology 2023Quote: ... 0.5% Triton X100 and protease inhibitor cocktail (Roche), pH 8 ...
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bioRxiv - Microbiology 2023Quote: ... as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA). DNA libraries were multiplexed and loaded on an Illumina MiSeq instrument according to manufacturer’s instructions (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... qPCR and qRT-PCR was performed using LightCycler 480 SYBR Green I Master (Roche). The thermal cycling conditions were 95°C for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... anti-DIG antibody (Anti-Digoxigenin-AP Fab fragments) and Chemiluminescent detection with CSPD following the instructions of the kit (all products and kits from ROCHE).
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bioRxiv - Microbiology 2023Quote: ... and reverse transcripted using the Transcriptor First Strain cDNA Synthesis Kit (Roche).
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bioRxiv - Microbiology 2023Quote: ... PCR reactions with a total volume of 25 μL included: 2× KAPA HiFi Hot Start Ready Mix (KAPA Biosystems), a final concentration of 0.1 mM for each primer and 7.5 μL of DNA sample ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 20 ml PBS plus two cOmplete Mini EDTA- free protease inhibitor tablets (Roche) and 25 μg/ml phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Microbiology 2023Quote: ... We recircularized linearized plasmid PCR products with a Rapid DNA Ligation Kit (Roche) and transformed plasmids into DH5α-competent cells (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... 1 mM EDTA, 0.1 % NP-40, 150 mM NaCl, 0.5 mM DTT, plus a protease inhibitor cocktail from Roche; used in pull-down experiments with the GFP-Trap resin of Proteintech) ...
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bioRxiv - Microbiology 2023Quote: ... GFP- or HA3x-tagged proteins were detected using α-GFP (1:1000; Roche) or α-HA3x (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... 12.5 μL KAPA HiFi HotStart ReadyMix (Roche Sequencing Solutions, Pleasanton, USA), 2 μM primers B341F (5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3’ ...
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bioRxiv - Microbiology 2023Quote: The RNA of all cell culture supernatants was extracted using High Pure Viral RNA kit (Roche, REF.11858882001). Viral RNA quantification in each sample was performed by quantitative reverse transcription PCR (RT-qPCR ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted with 12 ml of elution buffer (20 mM Tris, 500 mM NaCl, 6 M guanidine, 600 mM imidazole, 1 mM MgCl2 and 1X Roche protease inhibitor) and exchanged into #1 to #5 dialysis buffers in order to remove the imidazole and the Guanidine ...