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Citations for Roche :
8451 - 8500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and High Pure RNA Isolation Kit (Roche), treated by TURBO DNAse (Ambion ...
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bioRxiv - Microbiology 2023Quote: ... with the LightCycler 480 system® (Roche). RNA levels were normalised to GAPDH when indicated and analysed using the 2−ΔΔCT as described before (Livak and Schmittgen ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were quantified using KAPA Library Quantification Kits for Illumina Platforms (Kapa Biosystems; Wilmington, MA). Four uniquely dual indexed libraries ...
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bioRxiv - Microbiology 2023Quote: ... The Kapa Taq DNA polymerase (Kapa Biosystems) was used for amplification (denaturation ...
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bioRxiv - Microbiology 2023Quote: ... and complete protease inhibitor cocktail (Roche Applied Science) or 2% SDS and homogenized by either passing the solution through a 26-gauge needle or by sonication ...
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bioRxiv - Microbiology 2023Quote: ... We blocked the wells with 1% BSA (Fraction V, Roche) in DPBS (70 µl per well ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time PCR was carried out using FastStart Universal SYBR Green Master Mix (Roche) on a CFX 384 Bio-Rad thermal cycler (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... and biotin RNA labeling mix (Roche 11685597910).
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bioRxiv - Molecular Biology 2023Quote: ... on a LightCycler 480 II (Roche, Mannheim, Germany), using manufacturer’s recommended thermocycling conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... Expand™ Long Template PCR System (Roche) was used with the following thermocycling program ...
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bioRxiv - Molecular Biology 2023Quote: ... were lysed by sonication in ∼140 mL lysis buffer (50 mM Tris-HCl pH 8, 250 mM KCl, 1 mM TCEP) supplemented with protease inhibitor cocktail (Roche) and DNase I (5 μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... and 100 ng used to construct libraries for sequencing using the KAPA RNA HyperPrep kit (Roche Diagnostics) with 8 cycles of amplification ...
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bioRxiv - Microbiology 2023Quote: ... 0.1% Tween-20) supplemented with Protease Inhibitor (cOmplete, Roche) and lysed in a Precellys homogenizer (Bertin Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with a complete protease inhibitor cocktail (Roche). After sonication ...
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bioRxiv - Microbiology 2023Quote: ... titrated to pH 8.1 with phosphoric acid) with protease and phosphatase inhibitors added (Roche, CO-RO and PHOSS-RO) and sonicated ...
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bioRxiv - Microbiology 2023Quote: ... after resuspending samples with Stool Transfer and Recovery (STAR) buffer (Roche, Basel, Switzerland), the samples were repeatably bead beaten with beads in MP FastPrep-24 5G (MP Biomedicals ...
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bioRxiv - Microbiology 2023Quote: ... Library quantification was performed with qPCR using KAPA Library Quantification Kit for Illumina Platforms (KAPA/Roche). Libraries were normalized ...
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bioRxiv - Microbiology 2023Quote: ... 2 mg/ml lysozyme and protease inhibitors (Roche) per 1 L of culture ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.2) with protease and phosphatase inhibitors (Roche; 5892791001). 20 µg of each sample were denatured at 95°C for 5 min and separated by SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... RNA extraction was performed employing the High Pure RNA Isolation Kit (Roche), with an additional DNA digestion step that involved the addition of DNAse (Ambion™ ...
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bioRxiv - Neuroscience 2023Quote: ... 50 mM Tris-HCl (pH 8.0) with protease and phosphatase inhibitors (1X Roche Complete Mini ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by immunoblotting against GFP and against the loading control Pgk1 using mouse anti-GFP (11814460001, Roche), mouse anti-Pgk1 (459250 ...
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bioRxiv - Molecular Biology 2023Quote: ... The proliferative activities of VSMCs were quantified by BrdU incorporation using an enzyme-linked immunosorbent assay (ELISA) detecting kit (Roche, Mannheim, Germany) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... The resulting NGS adapter ligated cDNA was then amplified with KAPA HiFi HotStart ReadyMix using NGS indexing primers for 25 cycles (ROCHE). Amplified DNA fragments between 150∼500nt were size selected by gel extraction purification following gel-electrophoresis in a 4% SYBR-GOLD E-GEL ...
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bioRxiv - Molecular Biology 2023Quote: ... 1x cOmplete protease inhibitors (Roche Diagnostics, Mannheim, Germany)) for protein extraction ...
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bioRxiv - Molecular Biology 2023Quote: ... library concentrations were quantified using KAPA-QUANT qPCR kit (ROCHE). NGS reads were merged using BBMerge using a normal merge rate and then mapped onto the TnpB containing locus using Geneious mapper at Low Sensitivity to identify reRNA boundaries (Geneious 2023.0.1 (https://www.geneious.com)).
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... the proliferation of spheroids was measured using WST1 (Roche) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... and phosphatase inhibitors (PhosSTOP, Roche) directly to the well ...
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bioRxiv - Genomics 2023Quote: ... with protease (Complete, Roche) and phosphatase inhibitors (PhosSTOP ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with a cocktail of protease inhibitors (Roche Cat# 4693159001) and phosphatase inhibitors (Sigma-Aldrich Cat# P5726)) ...
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bioRxiv - Genomics 2023Quote: ... Real-time quantitative PCR was done with SYBR green (Roche) according to the manual with the primer sets for each gene as follows ...
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bioRxiv - Genomics 2023Quote: ... supplemented with EDTA-free cOmplete Mini Protease Inhibitors (Roche, Basel, Switzerland) and 0.5% BSA (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... The aorta was digested with LiberaseTM (50 μg/mL) (Roche Applied Science) for 1.5 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... phosphatase inhibitor (Roche), 0.4 U/ml benzonase (Merck ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 8.0) or Lysis buffer (1 % SDS, 50 mM Tris pH6.8 and 10 % Glycerol) supplemented with protease inhibitor cocktail (Roche) and subsequently quantified using Pierce BCA Protein Assay Kit (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... phosphatase inhibitor (Roche), 0.4 U/ml benzonase (Merck ...
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bioRxiv - Molecular Biology 2023Quote: ... Two days after transfection cells were washed twice with ice-cold PBS and scraped in ice-cold Co-IP buffer (10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, protein inhibitor cocktail (Roche)) supplemented with 0.5% Nonident P40 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg total RNA was reverse transcribed using the Transcriptor First Strand cDNA Synthesis Kit (Roche) with random hexamer primers according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and reverse transcribed using Transcriptor First Strand cDNA Synthesis Kit (Roche). cDNA was amplified by Q5® High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: Proteolysis with chymotrypsin (Roche) was performed by diluting the solutions to 1 M urea with 50 mM ammonium bicarbonate and adding the protease at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Molecular Biology 2023Quote: ... Detection was performed using alkaline phosphatase-conjugated anti-digoxigenin Fab fragments and the chemiluminescent substrate CSPD (Roche Applied Science). ImageJ analysis was performed to calculate the relative intensity of Maxi-probe detected bands with the corresponding control-probe detected bands.
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bioRxiv - Molecular Biology 2023Quote: ... Highly sensitive DNAprobes labelled with digoxigenin-dUTP were generated using the PCR digoxigenin probe synthesis kit (Roche Applied Science) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... the cDNA inserts of the plasmids were isolated and amplified by low-cycle (12x) PCR using a Kapa2G Robust Hotstart PCR kit (Roche, Ref #07961073001) according to specifications ...
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bioRxiv - Neuroscience 2023Quote: ... The libraries were quantified by fluorometry on a Qubit instrument (LifeTechnologies) and by qPCR with a Kapa Library Quant kit (Kapa Biosystems-Roche) prior to sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... The libraries were quantified by fluorometry on a Qubit instrument (LifeTechnologies) and by qPCR with a Kapa Library Quant kit (Kapa Biosystems-Roche) prior to sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 complete Mini Protease Inhibitor Tablet (Roche) per 10 mL) ...
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bioRxiv - Molecular Biology 2023Quote: Protease inhibitor (Protease inhibitor Cocktail Tablets, complete, Mini, EDTA-free Tablets) were purchased from Roche Diagnostics (Indianapolis ...
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bioRxiv - Molecular Biology 2023Quote: ... They were incubated with the primary antibodies (anti-HA, Roche, 11867423001 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% SDS) containing 2x Complete protease inhibitor cocktail (Roche). Lysates were then cleared of cellular debris by centrifugation at 21,000 x g for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Complete protease inhibitor cocktail (Roche) and PhosSTOP (Roche)) ...