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Citations for Roche :
701 - 750 of 7935 citations for S 6 Methoxy 2 3 dihydro 1H inden 1 amine hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... diluted to a 50nM concentration in hybridisation solution (10mM Tris-HCl (pH=7.4) and 0.5% blocking reagent (Roche) in 70% formamide ...
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bioRxiv - Biochemistry 2024Quote: ... cremoris samples were resuspended in 100 mM Tris-HCl (pH 8.5) with a protease inhibitor cocktail tablet (Roche) and 2% sodium dodecyl sulfate ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells grown until exponential phase were resuspended in Lysis Buffer (10 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.5X cOmplete™ (ROCHE), 0.5% (v/v ...
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bioRxiv - Biophysics 2024Quote: ... the cells were re-suspended in Tris-HCl buffer containing a complete EDTA protease inhibitor cocktail tablet (Roche) and lysed by sonication ...
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bioRxiv - Cancer Biology 2024Quote: ... lysed in cold lysis buffer (50 mM Tris–HCl [pH 7.5], 50 mM KCl, 5% glycerol, 0.4% Triton X-100, 1X Roche EDTA-free Complete Protease Inhibitor cocktail) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were lysed with 1% Triton X-100 in lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, Roche EDTA-free protease inhibitor cocktail and NEM) ...
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bioRxiv - Immunology 2023Quote: ... 3 mM ATP (Roche), 25 μg/ml MSU (InvivoGen) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... lumbar vertebrae 1 – 5 were incubated in 2% Collagenase P (Roche, Switzerland) for 30 minutes at 30° C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... pH 7.5) with the addition of 2 mg·ml-1 collagenase (Roche, Indianapolis). Defolliculated oocytes were injected with ENaC cRNAs into the cytosol (25 ng per oocyte in 50 nl of RNase-free water ...
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bioRxiv - Neuroscience 2020Quote: ... nuclei were stained with 4,6 diamine-2-phenylindole (1:25000, DAPI, Roche) in PBS 1X for 1 minute ...
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bioRxiv - Biophysics 2023Quote: ... and protease inhibitor tablets (1 per 2 L culture, Roche, Basel, Switzerland) and lysed via sonication ...
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bioRxiv - Neuroscience 2023Quote: ... 1 or 2 mL of the cOmplete His-Tag purification resin (Roche) equilibrated with the solubilization buffer 2 was added ...
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bioRxiv - Microbiology 2024Quote: ... 2) step - 1:0.85 using Kapa HyperPure Beads (Roche, cat. no. 07983298001). Sequencing was performed using Pair-end 2×100 cycle mode on the Illumina NovaSeq 6000 system (NovaSeq 6000 S1 Reagent Kit v1.5 200 cycles Illumina ...
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bioRxiv - Microbiology 2024Quote: ... and protease inhibitor tablets (1 per 2 L culture, Roche, Basel, Switzerland) and lysed via sonication ...
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bioRxiv - Genomics 2024Quote: ... 1 mM Tris(2-carboxyethyl)phosphine (TCEP) and complete protease inhibitor (Roche)) and added to the oligo-bound magnetic beads for 2 hours at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... then washed twice with Buffer 2 (0.05% w/v Saponin, 1× Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Cancer Biology 2020Quote: ... 80 µl of dephosphorylation solution (8 μl 10x dephosphorylation buffer, 2 μl RNasin Plus, 67 μl ddH2O, and 3 μl alkaline phosphatase (Roche, catalog no. 10713023001)) was added to the beads ...
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bioRxiv - Neuroscience 2022Quote: ... 20 mg protein was loaded onto 8-10% SDS-PAGE gels for electrophoresis and subsequently transferred (90 V, 2-3 h) onto PVDF western blotting membranes (Roche, Cat. No. 3010040001) using the Criterion™ System BioRad ...
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bioRxiv - Immunology 2021Quote: ... which were subsequently incubated with digestion buffer (IMDM supplemented with 2% FBS, 1 mg/mL Collagenase D [Roche], 2 U/mL DNase I [Life Technologies] and Dispase II [Roche]). The digested brain parenchyma ...
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bioRxiv - Systems Biology 2023Quote: ... 500 mM NaCl and 3 mM Dithiothreitol (DTT)) supplemented with 1 mg deoxyribonuclease I (DNaseI, Roche) and protease inhibitor 4-(2-Aminoethyl ...
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bioRxiv - Cell Biology 2024Quote: ... with primer set #3 (Supplementary Table 1) using KAPA High-Fidelity DNA Polymerase (KAPA Biosystems, KE2502). The forward and reverse primers contained BspEI and KpnII sites respectively ...
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bioRxiv - Cell Biology 2024Quote: One million cells were initially lysed in 0.5% CHAPS (3-cholamidopropyl dimethylammonio 1-propanesulfonate) (Roche; #10810118001) in PBS (1x ...
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bioRxiv - Cell Biology 2024Quote: ... and either pBABE-puro-F-NOMO1r (6 μg) or pBABE-puro-F-NOMO1r4-Mut (6 μg) using 27 µL of X-tremeGENE 9 (Roche). Supernatants were filtered after 72 h through a 0.45 μm filter unit ...
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bioRxiv - Cell Biology 2021Quote: ... 2.5 U/ml glucose-6-phosphate dehydrogenase (Roche), 2.5 U/ml hexokinase (Roche) ...
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bioRxiv - Biochemistry 2022Quote: ... Glucose-6-phosphate (G6P) (#10127647001) is from Roche. All other chemicals if not noted otherwise are from Sigma Aldrich.
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bioRxiv - Systems Biology 2022Quote: ... 6 cycles of preamplification with KAPA HiFi (Roche), and purification with Ampure XP beads (1:1 ratio ...
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bioRxiv - Developmental Biology 2022Quote: ... using FuGENE 6 transfection reagents (Roche, Basel, Switzerland). The cells were selected with puromycin (1 μg/ml ...
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bioRxiv - Biochemistry 2024Quote: ... 6 U/ml Pyruvate kinase (Roche, ref: 10128155001), 9 U/ml L-lactate dehydrogenase (Roche ...
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bioRxiv - Microbiology 2022Quote: Total RNA from 4 replicates of BCBL-1 WT clone B4 and 57KO clone #6 cells were isolated by TriPure Reagent (Roche) 24 h after induction with VA ...
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bioRxiv - Biophysics 2022Quote: ... the force was kept at 11 pN and Pif1 was added to the microfluidic chamber at a concentration of 6 nM and 1 mM ATP Lithium salt (Roche) in G4-Pif1 buffer ...
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bioRxiv - Biophysics 2021Quote: ... Pellets were suspended in 50 ml of denaturation buffer (8 M GdmCl, 50 mM Tris-HCl, pH 8.0) with one protease inhibitor tablet (Roche) per L medium ...
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bioRxiv - Biophysics 2020Quote: ... resuspended in buffer A (50 mM Tris-HCl pH 7.5 and 200 mM NaCl) with protease inhibitors (complete, Roche) and bacterial lysis was performed by sonication ...
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bioRxiv - Microbiology 2020Quote: ... bacteria were disrupted by sonication in a lysis buffer containing 10 mM Tris-HCl pH 7.4 (buffer A) and Complete Protease Inhibitor Cocktail (Roche). Cell debris was removed by centrifugation at 16,000 g at 4°C during 10 minutes and supernatants were collected as total extracts ...
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bioRxiv - Molecular Biology 2020Quote: ... and then lysed with IP lysis buffer (0.5% NP-40, 150 mM NaCl, 50 mM Tris HCl, pH 7.5, protease inhibitors [cOmplete, Roche], and phosphatase inhibitors [PhosStop, Roche]). Cell lysates were clarified by serial centrifugation (600 ×g for 3 min and 9300 ×g for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... then 10-fold diluted in TBS buffer (50mM Tris-HCl pH 7.5; 150mM NaCl) supplemented with cOmpleteTM protease inhibitors (Roche). ANTI-FLAG M2 Magnetic Beads (SIGMA ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were lysed in resuspension buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl and protease inhibitor cocktail (Roche) with sonication on ice ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were harvested using ice cold EBC buffer (140 mM NaCl, 50 mM Tris-HCl, 0.5% NP-40, 5 mM EDTA and protease inhibitor cocktail (Roche)) and briefly sonicated prior to 20 min centrifugation at 20,000 g ...
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bioRxiv - Molecular Biology 2021Quote: ... the nuclear fraction was resuspended in the TBS buffer (50 mM Tris•HCl, pH7.4; 420 mM NaCl; Complete protease (Roche), 1 mM PMSF ...
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bioRxiv - Neuroscience 2020Quote: ... cells were lysed in 300μl of SDS lysis buffer (0.5% SDS, 10mM EDTA, 50mM Tris-HCl) supplemented with protease inhibitor cocktail (Roche, 11836153001), sonicated for 10min in a Diagenode Bioruptor Pico ...
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bioRxiv - Molecular Biology 2020Quote: ... and resuspended in proteome lysis buffer (50 mM Tris HCl pH 8.0, 8 M urea, 75 mM NaCl, 100 mM sodium butyrate, cOmplete protease inhibitors – Roche). Cells were then mechanically lysed (MagNAlyzer Roche – 6000 rpm ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed in 1% Triton-TBS lysis buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1mM PMSF) supplemented with protease inhibitor cocktail (Roche). Total protein concentration was determined by bicinchoninic acid (Pierce) ...
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bioRxiv - Bioengineering 2020Quote: ... and 200 mg of fresh leaf pieces were homogenized with 800 μL of 20 mM Tris-HCL (pH 9.5) supplemented with protease inhibitor cocktail (Complete Mini, Roche) using a mortar and pestle ...
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Biochemical alterations precede neurobehavioral deficits in a novel mouse model of Friedreich ataxiabioRxiv - Biochemistry 2021Quote: ... All the pieces were placed in 1.5 ml screw cap polypropylene tubes in the presence of lysis buffer consisting in 50 mM tris(hydroxymethyl)aminomethane (Tris) HCl pH 7.5 containing a protease inhibitor cocktail (Roche). For each 100 mg of tissue ...
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bioRxiv - Microbiology 2021Quote: ... xanthus culture was harvested and the pellet resuspended in lysis buffer (50 mM Tris-HCl pH7.6, Protease inhibitor cocktail (Roche)) (1 ml per 80 mg cells) ...
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bioRxiv - Microbiology 2021Quote: ... and resuspended in MBP-lysis buffer (100 mM Tris-HCl pH 7.0, 200 mM NaCl) supplemented with EDTA-free protease inhibitor cocktail (Roche) and lysed using sonication ...
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bioRxiv - Cell Biology 2021Quote: ... in sonication buffer (10 mM Tris-Hcl pH 8.0, 0.25% SDS, 2mM EDTA, 1x Roche complete Protease Inhibitor (Roche # 11697498001) with setting “high”,15 x (30 sec on / 30 sec off) ...
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bioRxiv - Cell Biology 2021Quote: ... The cells pellet was resuspended in Lysis buffer (20mM Tris-HCl pH 7.5, 150mM NaCl, 1mM EDTA) containing protease inhibitor cocktail (Roche) and lysed using mechanical disruption (Isobiotec Cell Homogenizer) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were resuspended in lysis buffer (50 mM Tris-HCl buffer pH 7.4, 150 mM NaCl, 1x complete protease inhibitor cocktail (PIC) (Roche) and 1 mM PMSF) ...
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bioRxiv - Cell Biology 2021Quote: ... 300ul lysis buffer (50mM Tris-HCl, 150mM NaCl, 0.5% NP40 and 1mM EDTA) containing protease inhibitor (Roche, Basel, Switzerland) and 0.2M PMSF was added to each well and incubated on ice for 15 minutes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The imaginal discs were removed and suspended whole in 350μl of sucrose solution (250mM D-sucrose, 10mM Tris-HCl, 1mM. MgCl2, 1x protease inhibitors (Roche)) inside labelled 2ml dounce homogenisers (Sigma-Aldrich ...