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Citations for Roche :
951 - 1000 of 7935 citations for S 6 Methoxy 2 3 dihydro 1H inden 1 amine hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 100 mM KCl, 1 mM MgCl2, 0.5 mM β-mercaptoethanol, 0.1 mM GTP, 3 U/ml benzonase, 1X Roche Complete EDTA-free protease inhibitor ...
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bioRxiv - Cell Biology 2022Quote: ... and 100 µL protein breakage buffer (50 mM Tris-HCl pH 8.0, 1 mM EDTA, 20 mM Tris pH 9.5, 3 mM DTT, 1X cOmplete EDTA-free inhibitor cocktail [Roche]) were added to the samples ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were first mechanically dissociated with a scalpel into approximately 1-3 mm pieces and enzymatically digested in RPMI-1640 medium (Cytiva) containing 0.25 mg/ml DNase I (Roche) and 0.25 mg/ml Collagenase (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... 3 mM magnesium chloride) supplemented with 1 cOmplete protease inhibitor cocktail tablet per 50 ml of lysis buffer (Roche) and mechanically disrupted in a Dounce homogenizer ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected according to the manufacturer’s protocol at a µL lipofectamine: µg plasmid ratio of 3:1 (X-tremeGENE 9, Roche). After 48 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... pellets were resuspended in 100 μl of lysis buffer (50 mM Tris, pH 7.5, 1 mM EDTA, 3 mM DTT, and 1X cOmplete protease inhibitor cocktail [11836145001, Roche]) and lysed on a beadbeater for 5 min at room temperature with 100 μl of acid-washed glass beads ...
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bioRxiv - Molecular Biology 2021Quote: ... 6 mM creatine phosphate (Roche, Cat. No. 71519-72-7), 102 ng/μl creatine kinase (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesized with random p(dN)6 primers (Roche) and MMLV reverse transcriptase (Invitrogen) ...
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bioRxiv - Biophysics 2022Quote: ... C-33A cells were transiently transfected using FuGENE 6 (Roche) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was synthesized with random primers p(dN)6 (Roche) using SuperScript III Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid transfections were carried out by FuGENE 6 (Roche, E269A) as per the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... XG1 cells were supplemented with IL-6 (Roche, 3ng/ml).
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bioRxiv - Molecular Biology 2020Quote: ... 200 mM NaCl, 2 mM MgAc2, 1% [w/v] LMNG, 1 mM DTT, 1x complete EDTA-free protease inhibitor cocktail [Roche, Germany]). After 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... 200 mM NaCl; 2 mM MgAc; 1% [w/v] LMNG, 1 mM DTT, 1x complete EDTA-free protease inhibitor cocktail [Roche, Germany]) per 1 g of cell pellet and incubated for 30 min at 4°C ...
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bioRxiv - Physiology 2024Quote: ... Follicular membranes were removed from isolated oocytes by collagenase treatment (2 mg ml−1; type 1; Sigma-Aldrich or Collagenase-P from Roche) for 3 hr ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 2 x 109 cells were harvested and washed with equal volume of cold PBS and resuspended in 1 ml lysis buffer (1 x PBS, supplemented with 1% NP40 and 2 x cOmplete, EDTA-free protease inhibitor cocktail (Roche, 11873580001)) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then mechanically lysed (MagNAlyzer Roche – 6000 rpm, 1 min on, 2 min off) in the presence of 300 uL acid-washed glass beads (Sigma-Aldrich).
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bioRxiv - Biochemistry 2021Quote: ... 1 mM Tris(2-carboxyethyl)phosphin (TCEP) with complete EDTA-free protease inhibitor cocktail (Roche) and 10 µg/ml DNAseI (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM tris(2-carboxyethyl)phosphine [TCEP]) supplemented with EDTA-free protease inhibitors (Roche). The lysate was clarified by centrifugation (17,000 rpm for 1 h at 4°C ...
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bioRxiv - Immunology 2020Quote: ... diluted 2-fold with PBMC culture media and supplemented with 10% WST-1 reagent (Roche) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mM PMSF) supplemented with 1 protease inhibitor cocktail tablet (cOmplete-EDTA free, Roche, #11836170001) to a final volume of 50 mL ...
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bioRxiv - Developmental Biology 2023Quote: ... was diluted 1 in 50 in solution consisting of 2 % blocking reagent (Roche ref 11096176001), 1 % goat serum (Gibco ...
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bioRxiv - Immunology 2024Quote: ... The slides were counterstained with 4,6-diamidino-2-phenylindole (DAPI, 1 μg/ml; Roche, Switzerland) for 20 min ...
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bioRxiv - Genomics 2021Quote: ... rp49 5’-TAATACGACTCACTATAGGGCAGTAAACGCGGTTCTGCATG-3’ and 5’-CAGCATACAGGCCCAAGATC-3’) were transcribed using the Biotin RNA Labeling Mix (Roche) and T7 polymerase (Promega).
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bioRxiv - Microbiology 2021Quote: ... and then incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG; or anti-GFP, 1:1000, Roche #11814460001 in 3% BSA/TBS-T) at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Fixed embryos were homogenized in 2 ml cell lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.3% NP-40, 1x Roche Complete protease inhibitors cocktail) with a Dounce Homogenizer on ice and centrifuged 5 min 2,300g at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... iMN9D cells were lysed in cold immunoprecipitation (IP) buffer (50 mM Tris HCl pH 8, 150 mM NaCl, 0.1% Igepal CA-630) containing protease inhibitors (Roche Diagnostics, COEDTAF-RO). Following 30 min incubation at 4 °C on rotator ...
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bioRxiv - Genetics 2020Quote: ... TBS (Tris-buffered saline: 10 mM Tris-HCl, pH 7.4, 150 mM NaCl) containing 1X Western Blocking Reagent (Roche Applied Science, Inc.). Antibodies were diluted in TBS containing 0.5X Western Blocking Reagent + 0.1%Tween-20 ...
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bioRxiv - Biophysics 2021Quote: ... To isolate expressed aSyn the cell pellets were resuspended in lysis buffer (10mM Tris-HCl pH 8, 1mM EDTA and EDTA-free complete protease inhibitor cocktail tablets (Roche, Basel, Switzerland), 0.2 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The quenched PFA solution was then removed and the tissue was resuspended in ice-cold Hi-C Lysis Buffer (10mM pH=8 Tris-HCl, 10mM NaCl, 0.2% NP-40 and 1x Roche Complete protease inhibitor). The lysis was helped with a Dounce Homogenizer Pestle A on ice (series of 10 strokes in 10’ intervals) ...
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bioRxiv - Biochemistry 2021Quote: The pupae infected by the baculovirus were homogenized with a buffer solution (20 mM Tris-HCl, 150 mM NaCl, 5 mM DTT, 1mg/ml phenylthiourea, one inhibitor cocktail tablet (EASYpack, Roche, Basel, Switterland) per 50 ml solution ...
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bioRxiv - Developmental Biology 2023Quote: ... Fixed embryos were homogenized in 2 ml cell lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.3% NP-40, 1x Roche Complete protease inhibitors cocktail) with a Dounce Homogenizer on ice and centrifuged 5 min 2,300g at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... resuspended in Buffer A (50 mM Tris-HCl pH 8.0, 500 mM NaCl) supplemented with Protease Inhibitor Cocktail (Roche Diagnostics GmbH, Germany), phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed in ice-cold PBS, incubated in lysis buffer (8M urea in 50mM Tris/HCl, pH 7.8) supplemented with Phos-Stop tablets (Roche Diagnostics GmbH, Germany) and sonicated for 5 cycles of 5 s each at 10% amplitude (Branson Digital Microtip Sonifier 250-D ...
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bioRxiv - Biochemistry 2024Quote: ... Culture pellets were sonicated in 50 ml Lysis Buffer (10 mM Tris-HCl pH 7.9, 300 mM KCl, protease inhibitors from Roche according to manufacturer), spun at 18K rpm ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were resuspended in 150 μL of resuspension buffer (150 mM NaCl, 50 mM Tris-HCl, supplemented with cOmplete EDTA-free protease inhibitor cocktail [Roche], pH 7.4). For protein identification ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were lysed in cold lysis buffer (10mM Tris-HCl pH7.5, 10mM NaCl, 5mM MgCl2, 1mM EGTA, with Roche Protease Inhibitor #04693159001) and incubated on ice for 10 minutes to extract nuclei ...
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bioRxiv - Cell Biology 2022Quote: Frozen yeast lysate powder was resuspended in ice-cold lysis buffer (50M Tris-HCl, pH 7.4, 150mM NaCl, 0.05% Triton X-100, 1x Roche cOmplete Proteinase inhibitor cocktail) to a concentration of 6µg/µl and rotated for 10 min at 4°C before centrifugation at 3000 x g for 10min at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were pelleted and resuspended in 2× SDS-PAGE solvent (4% SDS, 20% glycerol, 0.1 M DTT, 0.125 M Tris-HCl pH 7.5 and Roche EDTA-free protease inhibitor cocktail) and boiled at 95°C for 5min ...
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bioRxiv - Physiology 2024Quote: ... Liver samples were dounced in Buffer A (250mM sucrose, 10mM Tris HCl pH7.5, 5mM MgCl2,100μM PUGNAc, and Roche EDTA-free protease inhibitor cocktail) with a loose pestle ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Crosslinked cell pellets were lysed in 5 mL cold lysis buffer (10 mM Tris-HCl, pH7.5; 10 mM NaCl; 5 mM MgCl; 0.1 mM EGTA; 1x Roche Complete protease inhibitor cocktail). Lysis was allowed to proceed for minutes on ice with intermittent gentle pipetting to obtain homogeneous suspensions of nuclei ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were scraped from the plate and lysed with 0.5% NP-40 buffer (10 mM Tris-HCl, 10 mM NaCl, 0.5% NP-40, freshly added Roche cOmplete protease inhibitor cocktail) for 15mins at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated in lysis buffer (8M urea in 50mM Tris/HCl, pH 7.8) supplemented with Phos-Stop tablets (Roche Diagnostics GmbH, Germany), sonicated and cleared by centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were resuspended in 150 µl resuspension buffer (150 mM NaCl, 50 mM Tris-HCl, supplemented with protease inhibitor cocktail [Roche], pH 7.4). CISSc sheath preparations were analyzed by negative stain EM imaging40 and mass spectrometry at the Functional Genomics Center Zürich.
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bioRxiv - Developmental Biology 2024Quote: ... Thawed cell pellets were then lysed (10mM Tris-HCL pH8, 10mM NaCl, 0.2% Igepal CA-630, Roche Complete EDTA-free Sigma #11836170001) and digested with 600U of DpnII (NEB) ...
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bioRxiv - Neuroscience 2021Quote: ... Labeled S and AS probes were generated using the DIG RNA Labeling Kit (SP6/T7) (Roche) per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Quantitative PCRs were carried out in triplicate using SYBR Green I Master Mix (S-7563, Roche) on a LightCycler 480 system (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: ... were prepared using the IP buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% TritonX-100, 0.5% Na-DOC, 1 mM EDTA, 2 mM PMSF and 1x Roche protease inhibitor cocktail). After sonification for 5 min and clarification with centrifuge at 16,000 g at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were stained with secondary antibodies (1:300) for 2 hours at room temperature and nuclei stained with DAPI (1:1000, Roche, Munich, Germany). The cells were mounted using the ProLong Diamond antifade mountant (Thermo ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were stained with secondary antibodies (1:300) for 2 h at room temperature and nuclei stained with DAPI (1:1000, Roche, Munich, Germany). Cells were mounted with ProLong Diamond Antifade Mountant (Thermo P36970 ...