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Citations for Roche :
651 - 700 of 7935 citations for S 6 Methoxy 2 3 dihydro 1H inden 1 amine hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... by scraping the cells in ice-cold PBS and lysing cell pellets in the lysis buffer (20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1.0% Triton X-100, and protease inhibitors; Roche). Supernatants and pellet fractions were separated by centrifugation at maximum speed for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Adipocytes were washed and lysed in a buffer (10mM Tris-HCl pH8.0, 10mM NaCl, 0.2% NP-40, 1x cOmplete Protease Inhibitor Cocktail from Roche) on ice for 30 min with shaking ...
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bioRxiv - Molecular Biology 2021Quote: ... The brain tissue was then homogenized on ice using a dounce homogenizer and diluted to 5 mL/g in homogenization buffer with RNase inhibitors (10mM Tris-HCl pH7.4, 0.8M NaCl, 1mM EGTA, 10% sucrose, 1X Roche protease inhibitor ...
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bioRxiv - Molecular Biology 2021Quote: ... The remaining pellet was incubated with 300μl of nucleosome release buffer (10mM Tris-HCl pH 7.5, 10mM NaCl, 0.2mM EDTA, 1x protease inhibitor cocktail (Roche)) at 4°C for 1 h ...
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bioRxiv - Genomics 2021Quote: ... and resuspended in ~250uL of Tep20 Buffer (10mM Tris-HCl pH 7.5, 0.1mM EGTA, 20mM NaCl and 1X Protease Inhibitor (Roche) added immediately before use ...
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bioRxiv - Molecular Biology 2021Quote: Total proteins from HEK293T cells were extracted using lysis buffer (150mM NaCl, 50 mM Tris-HCl, pH7.4, 0.5% Triton X-100) adding protease inhibitor cocktail (#11836153001, Roche). Prepared protein samples were separated by 12% or 15% SDS-PAGE and subsequently transferred onto a 0.2μm PVDF membrane (Pall) ...
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bioRxiv - Neuroscience 2020Quote: ... 25 mM Tris-HCl pH 7.6) containing phosphatase and protease inhibitors (PhosSTOP™, cOmplete™; both from Roche). Protein concentration was determined with the Pierce™ BCA Protein Assay Kit (Pierce™ ...
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bioRxiv - Cancer Biology 2021Quote: Transfected HeLa were harvested and lysed with lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5% NP-40, protease inhibitors tablet from Roche, 50 mM NaF ...
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bioRxiv - Neuroscience 2020Quote: ... brain from transgenic mice expressing different PrP deletion mutants was homogenized in 10 volumes of lysis buffer (50 mM Tris-HCl pH 8, 0.5% Na deoxycholate, and 0.5% Igepal, protease inhibitors (complete Mini, Roche) using TissueLyser LT for 5 min for 2 cycles ...
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bioRxiv - Plant Biology 2021Quote: Root tissue was ground in IP buffer (50mM Tris-HCL, pH7.5, 150mM NaCL, 10% glycerol, 0.1% NP40) containing cOmplete protease inhibitor (Roche). After clearing lysates at 12,000x g (10 min) ...
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bioRxiv - Microbiology 2021Quote: Purified BUNV RNPs were diluted in TNE buffer (100 mM Tris-HCl, 200 mM NaCl, 0.1 mM EDTA, pH 7.4, supplemented with protease inhibitors (Roche)) and mixed with indicated amounts of RNase A to a final volume of 20 μL ...
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bioRxiv - Plant Biology 2021Quote: ... were ground to fine powder in liquid nitrogen and homogenized in 575 μL of lysis buffer (10 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.15% IGEPAL (CA-630, Merk) and 1x cOmplete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Microbiology 2021Quote: ... cell extracts were lysed in TNN lysis buffer (100mM Tri-HCl pH8.0, 250 mM NaCl, 0.5% NP-40, and cOmplete protease inhibitor (Roche)) and centrifuged for 7 min at 15’000 rpm and 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Pellet was resuspended in 1mL of isolation buffer (225mM mannitol, 75mM sucrose, 30mM Tris-HCl pH 7.4, 0.1mM EGTA and protease inhibitors (Roche), and homogenized with a 25G needle at 4°C ...
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bioRxiv - Cell Biology 2021Quote: Cells transfected with indicated constructs were harvested and lysed in NETN buffer (10mM Tris-HCl [pH 8.0], 100mM NaCl, 1mM EDTA, and 0.5% NP-40) plus protease inhibitor (Roche) on ice for 30 min ...
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bioRxiv - Immunology 2020Quote: ... 10 million cells were harvested and lysed in EBC buffer (50 mM Tris-HCl pH 7.5, 120 mM NaCl, 0.5% NP40) with protease inhibitors (Complete Mini, Roche) for 30 min on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were lysed with NETN-300 buffer (20mM Tris-HCl Ph8.0, 300mM NaCl, 1mM EDTA, 0.5% Nonidet P-40) containing protease inhibitor cocktail (Roche), 3μM TSA and 50mM NAM on ice for 10min ...
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bioRxiv - Genomics 2020Quote: ... and resuspended in 1mL per 50E6 nuclei of Tep20 Buffer (10mM Tris-HCl pH 7.5, 0.1mM EGTA, 20mM NaCl, and 1X Protease Inhibitor (Roche) added immediately before use ...
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bioRxiv - Microbiology 2022Quote: ... cell extracts were lysed in TNN lysis buffer (100mM Tri-HCl pH8.0, 250 mM NaCl, 0.5% NP-40, and cOmplete protease inhibitor (Roche)) and centrifuged for 7 min at 15’000 rpm and 4°C ...
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bioRxiv - Microbiology 2021Quote: ... 1.1% Triton X-100, 1.2 mM EDTA, 20 mM Tris-HCl pH8.0, 167 mM NaCl, complete protease inhibitor [Roche]). Antibody (2 μg ...
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bioRxiv - Microbiology 2021Quote: ... 1.1% Triton X-100, 1.2 mM EDTA, 20 mM Tris-HCl PH8.0, 500 mM NaCl, complete protease inhibitor [Roche]), and LiCl (250 mM LiCl ...
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bioRxiv - Molecular Biology 2021Quote: Protein lysates were prepared from snap-frozen dorsal skin with urea buffer (8 M Urea, 40 mM Tris-HCl, 2.5 mM EDTA, pH 8.0) containing phosphatase (PhosSTOP, Roche) and protease inhibitors (cOmplete Protease Inhibitor Cocktail Tablet EDTA-free ...
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bioRxiv - Genomics 2021Quote: ... Pellets were resuspended in 500 μL cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP-40, 1X protease inhibitor (Roche)) and rotated for 30 minutes at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... washed twice with PBS and lysed in 5 ml lysis buffer (50 mM Tris-HCL, ph7,4; 500 mM NaCl, 0,2% SDS; 1x protease inhibitors (Roche), 20 mM DTT ...
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bioRxiv - Molecular Biology 2020Quote: ... washed twice with 1x PBS and resuspended in cold NP40 lysis buffer (10mM Tris-HCl pH 7.4; 10mM NaCl; 3mM MgCl2; 0.5% NP40 with freshly added protease inhibitors (Roche)) ...
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LptM promotes oxidative maturation of the lipopolysaccharide translocon by substrate binding mimicrybioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 20 mM Tris-HCl pH 8 containing a EDTA-free protease inhibitor cocktail (Roche). For the purification of protein samples that had to be analyzed both by reducing and non-reducing SDS-PAGE ...
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bioRxiv - Molecular Biology 2023Quote: ... and then lysed and harvested in ChIP lysis buffer (50 mM Tris-HCl pH 8.1; 0.5% SDS; 10 mm EDTA;100 mM NaCl, 1mM PMSF, Proteinase inhibitor Roche) by centrifugation for 6 min at 2,000 × g ...
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bioRxiv - Synthetic Biology 2023Quote: ... resuspended in 500 μL of Buffer B (30 mM Tris-HCl pH 7.9, 0.5 mM EDTA, antiprotease complete tablets (Roche)) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysis buffer (50 mM Tris-HCl, pH 7.5; 500 mM NaCl; 10 mM imidazole; 0.5 mM TCEP; 1X Roche cOmplete protease inhibitors ...
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bioRxiv - Cell Biology 2023Quote: ... and pellets resuspended in lysis buffer (50 mM Tris-HCl, pH 7.5; 150 mM NaCl; 0.5 mM TCEP, 1X Roche cOmplete protease inhibitors ...
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bioRxiv - Molecular Biology 2023Quote: ... A 2L spinner culture of asynchronous HeLa S3 cells was collected and homogenized using nitrogen cavitation in swelling buffer (20mM Tris- HCl [pH 7.5], 5mM KCl, 1.5mM MgCl2, 1mM DTT, 1x protease-inhibitor tablet(Roche)) ...
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bioRxiv - Molecular Biology 2022Quote: ... After exchanging the buffer with 100μl PI-lysis buffer (50mM Tris-HCl [pH7.5], 500mM NaCl, 2mM EDTA, 2mM DTT, 0.4%[w/v] SDS, cOmplete Protease Inhibitor Cocktail Tablet [Roche]), testes were homogenized using Bioruptor (Diagenode ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were lysed with 5mL cold lysis buffer (10 mM Tris-HCl, pH 7.5; 10 mM NaCl; 5 mM MgCl2; 0.1 mM EGTA; 1x complete protease inhibitor; 11836145001 Roche) for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed 2x with ice-cold PBS and then incubated for 10 min on ice with swelling buffer (10mM Tris-HCl [pH 7.5], 10% glycerol, 3mM CaCl2, 2mM, MgCl22, 0.5mM DTT, protease inhibitors [Roche] and 4 U/ml RNase inhibitors [RNaseOUT ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed twice with PBS and lysed for 20 min on ice in lysis buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40, supplemented with protease inhibitors [Roche]). Centrifugation at 13,000×g for 10 min at 4°C was then used to remove cell debris ...
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bioRxiv - Developmental Biology 2023Quote: Dissected embryonic brain structures were lysed in RIPA buffer (Tris HCl 1M, pH 7.7; NaCl 5M; EDTA 0.5M; Triton X-100) supplemented with protease inhibitors (Roche) and phosphatase inhibitors (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 10 µM Olaparib-containing medium for the 48 h before being washed with PBS and pre-extracted with pre-extraction buffer (10 mM Tris-HCl, 2.5 mM MgCl2, 0.5% NP-40, 100× Protease inhibitor cocktail (Roche)) for 5 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... after which they were resuspended in 1.0 ml of ice-cold lysis buffer (10 mM Tris-HCl, pH 7.7; 10 mM NaCl; 5 mM MgCl2; 0.1 mM EGTA; protease inhibitors [Roche]) and incubated on ice for 10 min before nuclei were pelleted (400 × g ...
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bioRxiv - Immunology 2022Quote: ... Cells were pelleted by centrifugation and resuspended in lysis buffer (40 mM Tris-HCl pH 8.0, 0.3 M NaCl, cOmplete protease inhibitor (Roche), 5 μg/mL DNase I ...
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bioRxiv - Neuroscience 2022Quote: ... For cellular extracts 50 μg of total protein isolated in NP-40 buffer (150 mM NaCl, 50 mM Tris-HCl pH 7.6, 0.5% NP-40, protease inhibitors (Roche)) was resolved in 10% SDS-PAGE gels and then transferred to a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2022Quote: ... 50mM TRIS-HCl pH 7.4) supplemented with protease and PhosSTOP phosphatase inhibitor (ref. 04 906 845 001 – Roche). Antibodies were diluted at 1:1000 in PBS - 0.1% Tween - 5% non-fat dried milk.
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bioRxiv - Cell Biology 2022Quote: ... Cells where lysed in immunoprecipitation buffer (50 mM Tris-HCl, 0.5% NP-40, and Roche protease inhibitor cocktail) and subjected to GFP trap (ChromoTek) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were thawed on ice and disrupted in lysis buffer (100 mM Tris-HCl pH 7.4, 0.5% Triton X-100, 150 mM NaCl, 13% w/v glycerol, protease inhibitor tablet, Roche) by bead beating (mini bead beater-16 ...
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bioRxiv - Molecular Biology 2024Quote: ... then resuspended in Lysis Buffer (20mM Tris-HCl, pH7.5; 500mM NaCl; 1.5mM MgCl2; 50mM Imidazole; 1X cOmplete EDTA-free Protease Inhibitor (Roche); 40U DNaseI (NEB)) ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 × 106 formaldehyde-cross-linked DN cells were lysed with 250 μl of ice-cold HiC lysis buffer (10 mM Tris-HCl pH 8.0, 10 mM NaCl, 0.2% IGEPAL CA630) containing protease inhibitors (Roche) for 15 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... SDS buffer (56 mM Tris-HCl pH6.8 buffer with 2.2 % SDS and 11 % glycerol) supplemented with protease inhibitors (cOmpleteTM Mini, Roche). Afterwards ...
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bioRxiv - Microbiology 2024Quote: Cell-and virus-associated proteins were solubilized in lysis buffer (30 mM NaCl, 50 mM Tris-HCl pH 7.5, 0.5% Triton X-100, 10 mM iodoacetamide, complete protease inhibitor [Roche]). Lysates boiled in 6X loading buffer (7 ml 0.5 M Tris-HCl/0.4% SDS ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 μl lysis buffer added (50 mM Tris-HCl pH 7.5, 75 mM NaCl, 0.25% NP-40, 2x protease inhibitors (Roche), and made 2 mM PMSF) ...
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bioRxiv - Microbiology 2024Quote: ... The pelleted cells were then resuspended in 500µl of Tris-HCl (pH8) supplemented with a mini cOmplete (Roche) protease inhibitors cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... The beads were washed 4x in Co-IP wash buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10% glycerol, 0.05% Triton-X100, Roche Complete Protease Inhibitor cocktail (1 tablet/50 mL) ...