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Citations for Roche :
901 - 950 of 7935 citations for S 6 Methoxy 2 3 dihydro 1H inden 1 amine hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... 1 mM 1,10-Phenanthroline and 2× Complete protease inhibitor cocktail and PhosStop cocktail (Roche). 2 mg total protein was incubated with 30 µl agarose-TUBE2 or agarose-control beads (tebu-bio ...
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bioRxiv - Neuroscience 2020Quote: ... The nuclei were stained with 4,6 diamine-2-fenilindole (1 μg/ml, DAPI, Roche). Cells were then washed and mounted with Fluorescent mounting medium (Dako).
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bioRxiv - Microbiology 2020Quote: ... The anesthetic was 2:1 Ketamine hydrochloride® (Pfizer, Spain):Diazepam ® (Roche, Spain).
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bioRxiv - Cell Biology 2023Quote: ... 1 mM tris(2-carboxyethyl)phosphine [TCEP]) supplemented with EDTA-free protease inhibitors (Roche). The cells were lysed via sonication and lysate was clarified by centrifugation (17,000 rpm for 1 hour at 4°C) ...
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bioRxiv - Neuroscience 2023Quote: ... samples were blocked for 2 hours in blocking solution (1% blocking reagent; (Roche, 11096176001) in malic acid buffer (0.15M maleic acid ...
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bioRxiv - Synthetic Biology 2023Quote: ... cell pellets were treated with 1 mL of 2 mg/mL lysozyme (Roche, Switzerland) and incubated at 30 °C for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... 1% penicillin-streptomycin) and treated with 2 units/mL of DNase I (Roche Diagnostics) for 15 minutes at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... 1% penicillin-streptomycin) and treated with 2 units/mL of DNase I (Roche Diagnostics) for 15 minutes at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... 250 mg of powder was thawed at 4°C followed by resuspension in 1 mL of HIP buffer (40 mM HEPES-KOH pH 7.5, 110 mM KOAc, 2 mM MgCl2, 1% Triton X-100, 0.1% Tween, 1x protease inhibitor cocktail [Roche], 1% solution P, 1 mM DTT). The lysate was next passed through a Whatman 25 mm GD/X Disposable filter (Cat No ...
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bioRxiv - Biochemistry 2022Quote: Proteins were extracted from adherent cells by scraping into extraction buffer (1× LysisM, 1× protease inhibitor cocktail, 2 x phosphatase inhibitor cocktail (all Roche), 2 mM sodium orthovanadate (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... and low-volume supernatants (90 μL media per well of a 48-well plate) were mixed 1:1 with 2× SDS/PAGE sample buffer containing Complete Mini EDTA-free Protease Inhibitor Mixture (Roche). In experiments where primary hMDMs were plated in a 24-well plate and infected with T4SS-Lp ...
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bioRxiv - Developmental Biology 2023Quote: ... sections were washed three times with TBST and then incubated with appropriate secondary antibodies (2 µg/mL in TBST / 1% bovine serum albumin (BSA)) and 1 µg/mL DAPI (Roche) for one hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were co-transfected with sgRNA expression vectors and lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 2:1:1 ratio using X-tremeGENE 9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were blocked for 2 hours at room temperature in 1% blocking solution (1% Western Blocking Reagent (WESTBL-RO, Roche) in TBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cells in 6-well plates were lysed with RIPA lysis buffer containing 1mM PMSF and 1% phosphatase inhibitor cocktail (PhosSTOP, Roche group, Swiss). The supernatant was collected after centrifuging at 14,000 rpm for 15 min at 4℃ ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted with 12 ml of elution buffer (20 mM Tris, 500 mM NaCl, 6 M guanidine, 600 mM imidazole, 1 mM MgCl2 and 1X Roche protease inhibitor) and exchanged into #1 to #5 dialysis buffers in order to remove the imidazole and the Guanidine ...
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bioRxiv - Genomics 2020Quote: ... embryos were sacrificed and DNA was extracted by exposure to lysis buffer (10mM Tris-HCl pH8, 50mM KCl, 1mM EDTA, 0.3% Tween 20, 0.3% Igepal) and proteinase K (Roche Diagnostics, Mannheim, Germany) for 2 h at 55°C ...
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bioRxiv - Biochemistry 2020Quote: ... and resuspended in 100 μL of 50 mM Tris-HCl (pH 7.6)/250 mM sucrose buffer supplemented with protease inhibitors (Roche Diagnostics GmbH). Cells were disrupted by 20 passages through a 27-gauge needle ...
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bioRxiv - Bioengineering 2020Quote: ... samples were prepared by combining 4 μL of medium per hydrogel and diluting it in 96 μL LDH (lactate dehydrogenase) storage buffer (200 mM Tris-HCl (Roche, 10812846001), 10% glycerol (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... About 20 g of bacterial cell pellet was resuspended in lysis buffer (100 mM Tris-HCl pH 7.5) supplemented with a tablet of EDTA-free cOmplete Protease Inhibitor S2 Cocktail Tablet (PIC) (Roche, Solna, Sweden) and DNase (PanReac Applichem ...
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bioRxiv - Genomics 2022Quote: The cell pellet was resuspended in 500 μL of cold permeabilization buffer (10 mM Tris-HCl pH 8.0, 10 mM NaCl, 0.2% IGEPAL CA-630, 100 μL of protease inhibitor cock-tail Roche Cat no. 11836170001) and placed on ice for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... 50 mM Tris-HCl (pH 8.0) and protease inhibitor (one tablet of Roche cOmplete™ mini per 10 ml lysis buffer) [22] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we homogenized dechorionated embryos with a mortar and pestle in ice-cold lysis buffer (50mM Tris HCl, pH7.5; 137 mM NaCl; 0.25% NP-40; Roche cOmplete protease inhibitors) before centrifugation at 18,000 rpm at 4°C and filtration through a 0.45 micron PVDF membrane.
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bioRxiv - Microbiology 2020Quote: ... cells were harvested by centrifugation and resuspended in 20 mL of lysis buffer (20 mM Tris-HCl, 500 mM NaCl [pH8], containing a protease inhibitor cocktail (Complete®, Roche)) ...
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bioRxiv - Genomics 2021Quote: ... 30μL from each was plated and diluted to 10ng/μL using 10mM Tris-HCl and sequencing libraries were prepared in triplicate using a miniaturized version of the KAPA HyperPlus kit (Roche, KK8514) with Illumina-compatible KAPA Unique Dual-Indexed Adapters (Roche ...
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bioRxiv - Biophysics 2023Quote: ... The resulting cell pellets were resuspended in Ni column buffer (25 mM Tris HCl, 50 mM NaCl, 30 mM imidazole pH 7.4) containing protease inhibitors (Roche Applied Science). Cells were lysed with 4 passes through an Emulsiflex C3 (Avestin) ...
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bioRxiv - Microbiology 2022Quote: ... cell pellet was resuspended in prechilled buffer A (20 mM Tris/HCl, pH: 8) supplemented with cOmplete™ protease inhibitor (Roche) and 5 mM EDTA ...
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bioRxiv - Cell Biology 2022Quote: ... The lyophilized oligo pool was resuspended in Tris-HCl (pH 8) and PCR-amplified using KAPA HiFi HotStart DNA Polymerase (Roche, KK2502) with 52 °C anneal and 15 sec extension (10 cycles) ...
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bioRxiv - Plant Biology 2024Quote: ... A total of 20 µl of anti-GFP beads was pre-washed with wash buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.6 mM PMSF, and 20 µM MG132 with Roche protease inhibitor cocktail) three times ...
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bioRxiv - Microbiology 2024Quote: Cell harvesting and disruption were performed with the multi shot cell disruptor at 20,000 psi using buffer A (100 mM Tris-HCl, pH 8.0) with cOmplete™ Protease inhibitor (Roche, Basel, Switzerland) for cell resuspension and disruption ...
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bioRxiv - Immunology 2023Quote: ... 1% NP-40, 150 mM NaCl, and 10 mM Tris-HCl [pH 8] supplemented with phosphatase and protease inhibitor cocktail tablets [Roche Diagnostics]). The cell lysates were cleared by centrifugation for 15 min at 40C ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 % NP40, 2.5 mM EDTA, 0.05 % NaDeoxycholate, 20 mM Tris-HCl pH 8, 1x of PhosSTOP, 1x Roche protease inhibitor mixture), and the TE buffer (same as for histones plus 1x PhosSTOP) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 mM Tris HCl pH 8.0) and subsequently resuspended in shearing buffer with freshly added protease inhibitors (cOmplete, EDTA-free, Roche Cat# 05056489001). ∼10-20 million cells per shearing tube were sheared by sonication using either the Bioruptor (Diagenode UCD-200 ...
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bioRxiv - Cancer Biology 2023Quote: ... resuspended at 10 mg/mL in AC/BT buffer (1.5M aminocaproic acid and 75mM Bis-Tris/HCl, pH 7.0, supplemented with Complete Mini Protease Inhibitor [Roche Diagnostics, Stockholm, Sweden]), and kept frozen at -80°C until analysis ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were centrifuged and the pellet was resuspended in Resuspension Buffer (25 mM Tris HCl pH 7.5, 250 mM NaCl) supplemented with one tablet of cOmplete protease inhibitor (Roche, Cat # 04693159001). Cells were then lyzed using a high-pressure Emulsiflex C5 homogenizer (Avestin) ...
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bioRxiv - Microbiology 2024Quote: ... 20 mM Tris-HCl, and pH 8.0) supplemented with protease inhibitor cocktail (Medchem Express, cat#HY-K0010) and PMSF (Roche, cat#10837091001). After ultrasonication ...
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bioRxiv - Microbiology 2024Quote: ... supernatant was collected and A549 cells infected with rLGTVT:prME R86 or Q86 were lysed in 350 µl of lysis buffer (50 mM Tris-HCl pH7.5 + 150 mM NaCl + 0.1% Triton X-100) complemented with 1x protease inhibitor (cOmplete™ ULTRA, Roche, Basel, Switzerland) on ice for 20 min ...
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bioRxiv - Neuroscience 2024Quote: Frozen cortical and hippocampal tissues were thawed and homogenized in ice-cold TBS buffer (50 mM Tris-HCl, pH 7.6, 150 mM NaCl, and protease inhibitor cocktail (Roche, Basel, Switzerland)) ...
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GRASP55 Safeguards Proper Lysosome Function by Controlling Sorting of Lysosomal Enzymes at the GolgibioRxiv - Cell Biology 2024Quote: ... in TBS-T buffer [50 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.1% Tween-20 (#A1389, AppliChem)] or with 5% BSA (#10735086001, Roche; #8076, Carl Roth) in TBS-T for phosphoprotein immunoblots ...
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bioRxiv - Molecular Biology 2022Quote: ... 72 °C for 10 s) using KAPA Hifi Hot Start Ready Mix (KAPA Biosystems KK2602) and primers (fw ...
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bioRxiv - Cell Biology 2022Quote: ... BrdU incorporation was carried out according to the manufacturer′s specifications (Cell Proliferation ELISA, Roche) with an incubation time of 5 hours as previously described.23 Quantification was performed by measuring the absorbance using Power Wave 340 ELISA reader from Bio-Tek.
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bioRxiv - Physiology 2024Quote: ... Minas Gerais) in an automated biochemical analysis equipment (Cobas Mira S, Roche®, Basel, Switzerland).
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bioRxiv - Biochemistry 2022Quote: ... 3 mg/ml Dispase II (Roche), and 1 mg/ml trypsin inhibitor (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... and ∼3 mg DNase I (Roche). MhOR5 was extracted using 0.5% (w/v ...
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bioRxiv - Developmental Biology 2022Quote: ... 3 µL 10% NP40 (Roche 11332473001), 3 µL 10% Tween-20 (Roche 11332465001) ...
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bioRxiv - Immunology 2023Quote: ... 3 IU/mL erythropoietin (EPO; Roche), 50 ng/mL stem cell factor (SCF ...
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bioRxiv - Molecular Biology 2023Quote: ... and dithiothreitol (Roche, 3483-12-3). Lysates were rocked at 4C for 20 min and centrifuged 10 min at 15,000g ...
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bioRxiv - Neuroscience 2024Quote: ... 3% SDC and PhosSTOP™ (Roche) by sonication at 40% amplitude for 4x10 sec ...
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bioRxiv - Biochemistry 2021Quote: ... except that all digests contained 200 µg/mL fibrils and 40 µg/mL pronase E and that the digest was stopped in each 20 µL aliquot with 2 µL protease inhibitor cocktail solution (1 tablet cOmplete EDTA-free Protease Inhibitor Cocktail, Roche, in 2 ml pure water) instead of PMSF solution ...
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bioRxiv - Cell Biology 2021Quote: ... The membranes were blocked with 3% skim milk in TBS for 30 minutes and then probed with mouse anti-GFP (1:1,000, Roche) or rabbit anti-aldolase (Mesén-Ramírez et al. ...