Labshake search
Citations for Roche :
7301 - 7350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed twice with ice cold PBS then scraped in 500 μL of PBS + protease inhibitors (PIs) (Roche). Cells were centrifuged at 8000 rpm (4◦C ...
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bioRxiv - Cancer Biology 2023Quote: ... GATA3 (L50-823; 1:1; Roche), E-cadherin (612130 ...
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bioRxiv - Biochemistry 2023Quote: ... MgCl2 (10 mM final concentration) and DNase1 (Roche) (8 U/mL final concentration ...
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bioRxiv - Developmental Biology 2023Quote: ... R-5’-GGGACGCAGCAACTGACATT-3’) was assessed by RT-qPCR using SyBR Green solution on a LightCycler480 (Roche). The cDNAs of every single cell were purified using the DNA Clean & Concentrator Kit (Zymo ...
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bioRxiv - Developmental Biology 2023Quote: ... the Anti-POD antibody (1:500; Roche) and TSA system (PerkinElmer ...
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bioRxiv - Developmental Biology 2023Quote: ... we used anti-dig-POD (Roche, cat ...
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bioRxiv - Developmental Biology 2023Quote: ... and for whole mount in situ hybridization (WISH) we used anti-digoxigenin-AP antibody (Roche cat ...
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bioRxiv - Developmental Biology 2023Quote: ... and 500 ng of random primer (Roche) at 25°C for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... and phosphatase inhibitors (EDTA-free Protease Inhibitor cocktail; #PHOSS-RO; Roche). Proteins were separated in SDS-PAGE and analyzed by western blotting by standard procedures ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with protease (cOmplete™, #COEDTAF-RO; Roche) and phosphatase inhibitors (EDTA-free Protease Inhibitor cocktail ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were resuspended in 200 µL low-salt Wash Buffer (20 mM HEPES-NaOH pH 7.5, 0.5 mM spermidine, 0.01% digitonin, 1x Roche protease inhibitor cocktail), the pA-MNase activated for 15 minutes at 0°C with 100 µL ice-cold Calcium Incubation Buffer (3.5 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... The libraries were quantified using the Kapa Library Quantification Kit Illumina Platforms (Kapa Biosystems). The denatured and diluted libraries were sequenced on a NextSeq 550 System (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA (3–4 μg) was used to prepare cDNA with the Transcriptor First-Strand cDNA synthesis kit (Roche). qPCR was performed with TaqMan gene expression assay primers (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Du145 (5 × 103) and 22Rv1 (1 × 104) cells were seeded on 96-well E-Plates (Roche). Proliferation was monitored every 1 h and time dependent cell index (CI ...
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bioRxiv - Cancer Biology 2023Quote: ... the quantification of ABCB1 copy number was performed with LightCycler 480 SYBR Green I Master mix (Roche Diagnostics, 04707516001). ABCB1 genomic amplification primer sequences are listed in Sup ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was PCR amplified with KAPA HiFi Hot Start Ready Mix (Roche, cat.no. KK2601) using cDNA FWD primer and cDNA REV primers (see Table 3) ...
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bioRxiv - Cancer Biology 2023Quote: ... the libraries were amplified by 12-rounds of indexing PCR (2X KAPA HiFi Hot Start Ready Mix, Roche, cat.no. KK2601). Library quality was assessed using Bioanalyzer DNA High Sensitivity chip (Agilent ...
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bioRxiv - Biochemistry 2023Quote: ... 60 µg/ml creatine kinase (Roche), 4.65 µg/ml myokinase (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... The regions of interest (Table S1) were enriched using SeqCap HyperChoice Library probes (NimbleGen; Roche Diagnostics, Jakarta, Indonesia). Libraries were sequenced on the NextSeq500 (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... The gDNA libraries were then generated with the KAPA Hyper Prep Kit (KAPA Biosystems). The regions of interest (Table S1 ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1% SDS) containing protease inhibitors (Roche) and phosphatase inhibitors (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: The cellular metabolic viability was measured by NADH-dependent mitochondrial dehydrogenase activity using Cell Proliferation Kit II (XTT) (Roche, Basel, Switzerland) according to the manufacturer’s protocol by measuring the absorbance at 492nm in BioTek Synergy HTX multimode reader ...
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bioRxiv - Cancer Biology 2023Quote: ... BrdU (colorimetric) kit (Roche, Basel, Switzerland) according to the manufacturer’s protocol with minor modification ...
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bioRxiv - Cancer Biology 2023Quote: ... with X-tremeGENE HP (Roche Diagnostics, Indianapolis, IN, USA), and the supernatant was harvested for lentiviral transduction ...
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bioRxiv - Cancer Biology 2023Quote: ... qRT-PCR analyses were performed using LightCycler® 480 SYBR Green I Master RT-PCR kits (ROCHE) on a Bio-Rad CFX Connect Real-Time system (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... qPCRs reactions were prepared in a 20 uL final volume containing Fast Start Universal SYBR Green Master (Rox) (Roche Applied Science), cDNA template ...
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bioRxiv - Cell Biology 2023Quote: ... EDTA-free Protease Inhibitor Cocktail (#11836170001, Roche) and pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... This plasmid was digested with PacI and transfected into HEK293 cells using X-tremeGENETM HP DNA transfection reagent (Roche, Basel, Switzerland) to produce infectious viral particles ...
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bioRxiv - Genomics 2023Quote: ... KAPA HiFi ReadyMix (Roche) was used to perform PCRs to amplify libraries in 25 μL reactions ...
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bioRxiv - Microbiology 2023Quote: ... qPCR was performed using FastStart Universal Probe Master (Roche) with primers and probes specific for late HIV-1 reverse transcription products (FW ...
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bioRxiv - Microbiology 2023Quote: ... 25 mM Tris (pH 8.0)) supplemented with proteinase and phosphatase inhibitor (Roche) for 30 min on ice ...
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bioRxiv - Immunology 2023Quote: ... Input DNA was obtained following the sample phenol-chloroform purification steps post overnight de-crosslinking of 10 μg of sonicated sample at 65 °C in the presence of 0.2 M NaCl and 10 μg/μl of RNase A and 0.8 μg/μl of Proteinase K (Roche). DNA was quantified with the Qubit dsDNA HS Assay Kit (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... Magnus Fontes (Institut Roche), Gunilla Karlsson Hedestam (Karolinska Institutet) ...
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bioRxiv - Immunology 2023Quote: ... Apoptosis also was detected using a terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick end-labeling (TUNEL) assay with Cell Death Detection Kit (Roche, Germany). Nuclei were stained with 4-6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2023Quote: ... Aortas were manually homogenized and digested for 20 minutes at 37°C in RPMI with 1 mg/mL of collagenase A and collagenase B and 0.1 mg/mL DNAse I (Roche). Kidneys were homogenized using the Miltenyi gentleMACS Dissociator and digested for 20 minutes at 37°C in RPMI with 2 mg/mL collagenase D (Roche ...
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bioRxiv - Immunology 2023Quote: ... Retinas of each model were pooled and digested in 1.5 mg/ml collagenase A and 0.4 mg/ml DNase I (Roche) for 45 min at 37°C with agitation ...
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bioRxiv - Immunology 2023Quote: ... 12.5 µl KAPA HiFi HotStart ReadyMix (KAPA Biosystems, KK2602). The PCR reaction was performed as follows ...
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bioRxiv - Immunology 2023Quote: ... brains and spinal cords were cut into pieces and digested for 30 min at 37 °C with DNase I (Sigma-Aldrich or Roche) at a concentration of 1 mg/ml and collagenase D (Roche ...
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bioRxiv - Immunology 2023Quote: ... Serum lipid panel was measured using Cobas b 101 POC system and its lipid disc (both from Roche Diagnostics). For liver cholesterol measurements ...
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bioRxiv - Immunology 2023Quote: ... 1 mM DTT) supplemented with phosphatase inhibitor (Roche, cat ...
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bioRxiv - Immunology 2023Quote: ... and protease inhibitor cocktail (Roche, cat. No. 11836170001) followed by incubation on ice for 30 min ...
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bioRxiv - Immunology 2023Quote: LightCycler 480 II Real-Time System (Roche, Basel, Switzerland), or Applied Biosystems 7500 Fast Real-Time PCR System (ThermoFisherScientific ...
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bioRxiv - Immunology 2023Quote: ... and phosphatase inhibitor (Roche, Switzerland). Total protein was quantified using a bicinchoninic acid (BCA ...
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bioRxiv - Immunology 2023Quote: ... then enzymatically dissociated (0.05mg ml-1 Liberase DL (Roche), 0.05 mg ml-1 Liberase TL (Roche) ...
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bioRxiv - Immunology 2023Quote: ... 0.05 mg ml-1 Liberase TL (Roche), 0.2 mg ml-1 Dnase I (Roche ...
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bioRxiv - Immunology 2023Quote: ... protease inhibitor (Roche, Switzerland), and phosphatase inhibitor (Roche ...
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bioRxiv - Immunology 2023Quote: ... the livers were cut and separated with forceps and digested in 3mg/mL Liberase (589994, Roche ® Life Science) and 3mg/mL DNase I (Roche ...
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bioRxiv - Immunology 2023Quote: ... sequencing libraries were prepared using KAPA Hyper Prep Kit (KAPA Biosystems). Sequencing of the cDNA libraries was performed by HiSeq2500 (Illumina).
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bioRxiv - Immunology 2023Quote: Cell lysates were prepared in RIPA buffer supplemented with complete protease-inhibitor cocktail (Roche) and mixed in equal amounts with Laemmli buffer (Roth ...
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bioRxiv - Immunology 2023Quote: ... The transcripts for genes of interest were measured by real-time qPCR with a Lightcycler 480 II system (Roche) and a Brilliant III SYBR Green Master mix (Agilent ...