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Citations for Roche :
7551 - 7600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Dispase II (2 U/ml; Roche, 04942078001), Collagenase D (2 U/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were brought to room temperature and washed twice in TNT before being incubated in blocking buffer (TNT+5% sheep serum+1% Roche blocking reagent) for 1 hour ...
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bioRxiv - Genetics 2023Quote: GDF15 measurements in both the HG vs control study and in Generation Scotland were measured on a Cobas e411 analyser (Roche Diagnostics, Basel, Switzerland) using the manufacturer’s reagents and quality control material ...
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bioRxiv - Genomics 2023Quote: ... resuspended in 30 ml of Solution A supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and 0.175x cOmplete™ (Mini, EDTA-free) protease inhibitor cocktail (Roche) in a 50 ml Falcon tube and disrupted by vortexing with an equal volume of glass beads (425-600 μm ...
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bioRxiv - Microbiology 2023Quote: ... 1% NP-40) supplemented with 0.25 mg/ml lysozyme and mini protease inhibitor cocktail (Roche). Samples were left on ice for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... 1% NP-40) supplemented with 0.25 mg/ml lysozyme and mini protease inhibitor cocktail (Roche). Samples were left on ice for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Cas12a-HA was detected using horseradish peroxidase (HRP)-conjugated HA antibody (Roche) at 1:5000 dilution ...
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bioRxiv - Genetics 2023Quote: Total RNA was extracted using the TriPure Isolation Reagent (Roche). Reverse transcription and quantitative real-time PCR were performed with PrimeScriptTM RT Master Mix (Takara ...
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bioRxiv - Genomics 2023Quote: ... 25 mM NaCl) with 200µg/ml Proteinase K (3115879001, Roche), incubating at 37°C for 30 min ...
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bioRxiv - Genetics 2023Quote: ... 2nd Generation (Roche, cat#03353621001) for cDNA production paired to Phusion High-Fidelity DNA Polymerase (New England BioLabs ...
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bioRxiv - Genomics 2023Quote: Total RNA were isolated using TriPure Isolation Reagent (Roche). For reverse transcription ...
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bioRxiv - Genomics 2023Quote: ... and 1X cOmplete mini EDTA-free protease inhibitor cocktail (Roche)) and cells were lysed by bead-beating for 5min at RT ...
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bioRxiv - Genomics 2023Quote: ... PCR amplifications were performed with Expand Long Template Enzyme mix (Expand Long Template PCR system, Roche). Oligos are available in Table S4.
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bioRxiv - Genomics 2023Quote: ... and PCR using NEB ultra mastermix (M0544S) and 10X KAPA Library Amp Primer Mix (Kapa Biosystems, KK2623). Samples were sequenced using a NovaSeq SP kit (Illumina ...
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bioRxiv - Genomics 2023Quote: ... 2 μg of RNA was treated with DNase I for 30 minutes (#04716728001; Roche) and subsequently treated with 1 μl 25 mM EDTA at 70 °C for 10 minutes to inactivate DNase I ...
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bioRxiv - Genomics 2023Quote: ... was used for the 121 epegRNA small scale screen and KAPA HiFi HotStart ReadyMix (Roche Diagnostics, Cat. No. KK2602) was used for amplification in the large scale screen using the recommended protocol with a 65C annealing temperature for the KAPA2G Robust PCR and the KAPA HiFi PCR ...
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bioRxiv - Genomics 2023Quote: ... KAPA2G Robust HotStart ReadyMix (Roche Diagnostics, Cat. No. KK5702) was used for amplification using the recommended protocol with a 60C annealing temperature and a 30 second extension time ...
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bioRxiv - Genomics 2023Quote: ... To correct ONT reads another library using the same HMW sample as above was constructed for shotgun sequencing with the Hyper Library construction kit from Kapa Biosystems (Roche, Basel, Switzerland) and sequencing was performed using an Illumina NovaSeq at the Roy J ...
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bioRxiv - Genomics 2023Quote: ... 5 mM 2-mercaptoethanol and cOmplete Protease Inhibitor Cocktail (Roche, no. 11697498001). The cell suspension was subjected to sonication (Qsonica ...
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bioRxiv - Genomics 2023Quote: ... Library concentration was assessed using both Qubit fluorometric quantification and the qPCR-based KAPA Library Quantification Kit (Roche, KK4824). Appropriate fragment size distributions were confirmed by running each sample on an Agilent Bioanalyzer using the DNA High-Sensitivity Kit (Agilent ...
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bioRxiv - Genomics 2023Quote: ... the libraries were amplified using the KAPA Library Amplification Kit (Roche, KK2702) with 12 PCR cycles for amplification ...
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bioRxiv - Genomics 2023Quote: ... Libraries were generated using the KAPA Hyper Prep DNA kit (Roche Sequencing and Life Science) and then shipped frozen to the University of California ...
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bioRxiv - Genomics 2023Quote: ... Library Enrichment was performed in a 30 μl reaction volume containing 20 μl DNA from the previous step and the following final concentrations: 1X KAPA buffer (Roche #KK2502), 0.3 mM each dNTP (Roche #KK1017) ...
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bioRxiv - Genetics 2023Quote: ... The molar concentration of the pool was measured using the KAPA Illumina Library Quantification Kit (KAPA Biosystems, Wilmington, MA) and the pool was sequenced on one lane of the Illumina HiSeq 4000 sequencer with a 75 bp Paired-End SBS chemistry (Illumina).
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bioRxiv - Genetics 2023Quote: ... AB individuals with the shortest wild introgressed fragment were selfed and the progeny was genotyped by High Resolution Melting (HRM) on a LightCycler 480 Real-Time PCR (Roche Diagnostics, Meylan, France) to identify recombinants with shorter introgressions ...
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bioRxiv - Immunology 2023Quote: Cells were isolated from the brain by mechanical disruption followed by collagenase D digestion (Roche Pharmaceuticals, Mannheim, Germany). The resulting cell suspension was resuspended in 40% Percoll (GE Healthcare Biosciences ...
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bioRxiv - Immunology 2023Quote: Human TGs were measured using the Cobas c 501 analyzer (Roche Diagnostic). For this purpose ...
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bioRxiv - Microbiology 2023Quote: ... A total amount of 1010 bacteria were pelleted at 4°C and resuspended in 1 mL of buffer A (20 mM Tris-HCl pH 8, 200 mM MgCl2) supplemented with protease inhibitor cocktail (PIC, Roche) and lysozyme to a final concentration of 0.5 mg/mL ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.4) containing one EDTA-free protease inhibitor pill (Roche) per 25 mL and stored at −80 °C.
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bioRxiv - Genomics 2023Quote: ... 0.5 mM each dNTP (Roche #KK1017), 0.25 mM ATP (NEB #P0756S) ...
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bioRxiv - Genomics 2023Quote: ... Genomic libraries were prepared with the HyperPrep Library construction kit from Kapa Biosystems (Roche, Basel, Switzerland). Libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Genomics 2023Quote: ... supplemented with PhosStop Tablet (Roche), Aprotinin (Sigma Aldrich) ...
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bioRxiv - Genomics 2023Quote: ... KAPA2G Robust HotStart ReadyMix (Roche Diagnostics, Cat. No. KK5702) was used for the 121 epegRNA small scale screen and KAPA HiFi HotStart ReadyMix (Roche Diagnostics ...
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bioRxiv - Genomics 2023Quote: ... was mixed 1:50 in Dig-300 buffer (without digitonin, 20 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 0.5mM spermidine, Roche Complete Protease Inhibitor EDTA-free) and beads were resuspended in 100 uL and nutated at room temperature for 1 hour ...
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bioRxiv - Genomics 2023Quote: ... KAPA2G Robust HotStart ReadyMix (Roche Diagnostics, Cat. No. KK5702) was used for amplification using the recommended protocol with a 60C annealing temperature and a 30 second extension time for this second PCR ...
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bioRxiv - Genetics 2023Quote: ... incubated the islets for 10 min at 37°C in 2 mL PBS with 4U Dispase I (Roche Diagnostics) / 2U DNase I (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... we washed pupal abdomens by replacing PBS with 1ml of Wash+ buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine with Roche complete EDTA-free protease inhibitor) and centrifuging twice at 12000 g for 5 minutes ...
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bioRxiv - Genetics 2023Quote: ... Library concentration was quantified with a KAPA Library Quantification Kit (Roche) and quality control was performed with the High-Sensitivity DNA Analysis kit in a Bioanalyzer 2100 (Agilent) ...
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bioRxiv - Genetics 2023Quote: ... RT-PCR was performed using KAPA HiFi HotStart ReadyMix (Roche) under conditions that resulted in 1% of the concentration of the original cDNA solution ...
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bioRxiv - Genetics 2023Quote: ... and quantified on a Light-Cycler 480 (Roche). Prior to RNA-seq library construction ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 mM TCEP and 10 % glycerol) with cOmplete EDTA-free Protease Inhibitor Cocktail (Roche). After lysate centrifugation at 48,384 g for 45 min at 4°C ...
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bioRxiv - Genomics 2023Quote: ... 900 ng from the breeding population) were used in a liquid-phase capture (SeqCap EZ developer, IRN 6089042357, OID35086, Roche Nimblegen). The captured material was amplified and sequenced on an Illumina HiSeq 4000 PE100 at the Centre d’Expertises et de Services Génome Québec (Montréal ...
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bioRxiv - Genetics 2023Quote: ... then washed 3X in PBS containing Complete Mini protease inhibitor (Roche). Worms were briefly spun down ...
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bioRxiv - Genetics 2023Quote: ... Next, 10 volumes of PBS (containing 1.1% formaldehyde, Complete Mini protease inhibitor (Roche) and phosphatase inhibitor cocktail (Calbiochem) ...
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bioRxiv - Genetics 2023Quote: ... and KAPA Library Quantification Kit (Kapa Biosystems), respectively ...
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bioRxiv - Genetics 2023Quote: ... The anti-HA (Roche 12CA5, 1:10,000) monoclonal antibody was used as previously described (52) ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM EDTA, 0.1% NP-40, 1 mM DTT, 1 mM PMSF, 1.3 mM benzamidine, protease inhibitor tablets [Roche]) and eluted with maltose (20 mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... 2.7 mM KCl, 0.1% NP40, 1 mM EDTA, 1mM DTT, 1 mM PMSF, 1.3 mM benzamidine, protease inhibitor tablets [Roche]) and eluted with glutathione (50 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... α-Ape1 (1:5000) (Hamasaki et al., 2003), α-Prb1 (laboratory stock, 1:5000) and α-GFP (Roche, 11814460001, 1:1000), were used as primary antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... SRA01/04 cell lysates were obtained using RIPA lysis buffer (Beyotime) supplemented immediately before use with 10 μl ml-1 phosphatase and protease inhibitors (PhosSTOPTM, Roche). The bicinchoninic acid assay (Beyotime ...