Labshake search
Citations for Roche :
451 - 500 of 7935 citations for S 6 Methoxy 2 3 dihydro 1H inden 1 amine hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... 2 mio cells were lysed in 100 μl Buffer-B (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS, 1x Roche cOmplete protease inhibitors) and sonicated in a microtube (Covaris ...
-
Glucocorticoid receptor collaborates with pioneer factors and AP-1 to execute genome-wide regulationbioRxiv - Genomics 2021Quote: ... followed by addition of 100 μL ChIP Elution Buffer (50 mM Tris-HCl, pH 7.5 @ RT, 10 mM EDTA, 1% SDS) and 20 μL Pronase (Roche, 20 mg/mL) for incubation at 42°C for 2 hours ...
-
bioRxiv - Molecular Biology 2022Quote: ... ESCs or HEK293T cells were harvested and lysed in TEN buffer (50 mM Tris-HCl, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 0.5% Na-Deoxycholate, supplement with Roche cOmplete Protease Inhibitor). The lysates were quantified by the Bradford method and equal amount of proteins were loaded for Western blot assay ...
-
bioRxiv - Molecular Biology 2024Quote: ... Nuclear pellet was suspended in 1 mL of TAP buffer (100 mM NaCl, 20 mM Tris-HCl pH 8, 2.5 mM EDTA, 10 % glycerol, 1 % Triton, 1x of PhosSTOP, 1x Roche protease inhibitor mixture) and given 20 strokes with the Dounce Homogenizer ...
-
bioRxiv - Microbiology 2023Quote: ... 50 mM Tris-HCl, 10% glycerol, 1% IGEPAL [Sigma, St Louis, MO], and cOmplete EDTA-free protease inhibitor cocktail [Roche] [pH 7.4]). Cells were vortexed ...
-
bioRxiv - Genomics 2023Quote: ... 3 million crosslinked cells were resuspended in 1 mL of ice-cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP-40, and 1× Roche protease inhibitors Cat#11697498001) and rotated at 4 °C for 30 minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... and lysed in immunoprecipitation buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% TritonX-100 supplemented with Roche protease inhibitor cocktail). Lysates were centrifuged (5,000x g ...
-
bioRxiv - Microbiology 2022Quote: ... The cell pellet was washed once with 50 mM Tris-HCl (pH 7.0) and resuspended in 1 mL of 50 mM Tris-HCl (pH 7.0) containing protease inhibitors (cOmplete, Roche Applied Science, Mannheim, Germany). The cells were disrupted with glass beads using a FastPrep system (Qbiogene ...
-
bioRxiv - Neuroscience 2023Quote: Lysates from cultured neurons were collected in NP40 lysis buffer (150 mM NaCl, 100 mM Tris-HCl, 1% NP40 in PBS) containing 1X protease inhibitors (cOmplete ULTRA tablets, Roche, cat# 05892970001). Samples were run on mini-protean TGX gels (Biorad ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... subsequently resuspended in 200µl of Lysis Buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl pH 8, 1x cOmplete™, EDTA-free Protease Inhibitor Cocktail (Roche #04693132001)) and frozen with liquid nitrogen ...
-
bioRxiv - Molecular Biology 2020Quote: ... and incubated with intermittent shaking (30 s shaking, 30 s off) for 15 min with 15 units of DNAse I (Roche) to remove DNA traces ...
-
bioRxiv - Microbiology 2020Quote: ... followed by 45 cycles of amplification under the following conditions: 94°C for 15 s and 60°C for 60 s in a LightCycler 96 (Roche).
-
bioRxiv - Microbiology 2021Quote: ... followed by 95 °C for 2 min and then 45 cycles of 95 °C for 15 s and 60 °C for 30 s using a Light Cycler 480 (Roche).
-
bioRxiv - Microbiology 2023Quote: ... followed by 95 °C for 2 min and then 45 cycles of 95 °C for 15 s and 60 °C for 30 s using a Light Cycler 480 (Roche).
-
bioRxiv - Biophysics 2020Quote: ... gamma-S-ATP was added to 100μM (Roche) (ATP concentration is 500μM ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Roche, #11465007001) and CellTiter-Glo Luminescent Cell Viability (CTG ...
-
bioRxiv - Biochemistry 2020Quote: For Figures 2 and 6: Cells or embryos were lysed in RIPA buffer supplemented with a cocktail of protease inhibitor (Roche). Total protein was resolved on SDS polyacrylamide gels (Bio-Rad ...
-
bioRxiv - Plant Biology 2022Quote: ... Glucose content was then measured by spectrophotometry after a 2 enzymes reaction with hexokinase and glucose-6-phosphate dehydrogenase (Roche).
-
bioRxiv - Evolutionary Biology 2022Quote: ... Embryos were then washed with wash buffer and their nuclei stained with 5 ug/mL DAPI (4′,6-diamidino-2-phenylindole, Roche) prepared in wash buffer for 10 minutes at 30 ºC.
-
bioRxiv - Neuroscience 2023Quote: ... diluted in PBS for 2 hours at room temperature after which they were counterstained with 300 nM 4’,6’-diamino-2-phenylindole (DAPI; 10236276001, Roche) for 5 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were washed three times with 1X PBS before nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, Roche) for 10 min at RT ...
-
bioRxiv - Microbiology 2020Quote: ... bacteria were harvested by centrifugation at 6,000 g for 10 min at 4°C and resuspended in L Buffer (25 mM Tris-HCl, 500 mM NaCl, 10 mM Imidazole, 1 mM PMSF, 5 % Glycerol, pH 8, containing Roche protease inhibitor cocktail). Bacteria were then lysed by sonication ...
-
bioRxiv - Developmental Biology 2020Quote: ... Pelleted nuclei were resuspended in 333 μl of nuclear lysis buffer (50 mM Tris-HCl pH 7.5, 10 mM EDTA, 1% SDS, 1x Roche Complete protease inhibitors cocktail), kept 5 min on ice and diluted with 667 μl of ChIP dilution buffer (16.7 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were harvested and resuspended in lysis buffer A (50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM PMSF, and Roche EDTA free protease inhibitor). Following sonication and centrifugation ...
-
bioRxiv - Biochemistry 2020Quote: ... The spheroplasts were resuspended in ice-cold D-buffer (0.6 M sorbitol, 10 mM Tris-HCl pH 7.4) containing protease inhibitors (complete protease inhibitor cocktail, Roche; 1 tablet per 50 mL), and lysed by 10 strokes in a Dounce homogenizer ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were lysed in IP buffer (150 mM NaCl, 10mM Tris-HCl pH 8, 1 mM DTT, 0.2% NP-40, 1x protease inhibitor [Roche], 1x phosphatase inhibitor [Roche]), sonicated and centrifugated for 10 minutes at 14,000 RPM at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... then lysed in ice-cold NP-40 lysis buffer (25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% NP-40, 5% glycerol, Roche cOmplete protease inhibitor cocktail) by trituration followed by incubation on ice for 10 minutes ...
-
bioRxiv - Neuroscience 2023Quote: ... Pellets were resuspended in lysis buffer (20mM Tris-HCL pH 8.0, 300mM NaCl; 4ml/100ml culture) with freshly added protease inhibitors (Roche #11697498001: 1 tablet/50ml) and lysozyme (0.1mg/ml) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Pelleted nuclei were resuspended in 333 µl of nuclear lysis buffer (50 mM Tris-HCl pH 7.5, 10 mM EDTA, 1% SDS, 1x Roche Complete protease inhibitors cocktail), kept 5 min on ice and diluted with 667 µl of ChIP dilution buffer (16.7 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biophysics 2024Quote: ... The pellet was resuspended in 50 ml lysis buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA, Roche Complete protease inhibitor cocktail) and the lysate was boiled for 20 min ...
-
bioRxiv - Neuroscience 2024Quote: ... were homogenized with a pre-chilled Dounce homogenizer using RIPA buffer (50 mM Tris-HCl, 250 mM sucrose, 1 mM EDTA, and Roche protease inhibitor tablet). Tissue lysates were cleared by cold centrifugation ...
-
bioRxiv - Cell Biology 2022Quote: ... DNA (1 μg) was mixed with 3 μl Fugene6 (Roche, #11836145001) in 200 μl opti-MEM (Gibco ...
-
bioRxiv - Neuroscience 2021Quote: ... 10 mM Tris-HCl pH 8.1) supplemented with cOmplete protease inhibitor cocktail (Roche) using the M220 focused-ultrasonicator (Covaris ...
-
bioRxiv - Microbiology 2021Quote: ... 10 mM Tris-HCl pH 7.4) supplemented with cOmpleteTM Protease Inhibitor Cocktail (Roche) and subjected to 2 freeze and thaw cycles ...
-
bioRxiv - Cell Biology 2021Quote: ... and 50 mM Tris-HCl (pH 8.0)] containing a protease inhibitor cocktail (Roche). Anti-FLAG immunoaffinity columns were prepared using anti-FLAG M2 affinity gel (Sigma ...
-
bioRxiv - Developmental Biology 2021Quote: ... 50 mM Tris-HCl pH 8,0) supplemented with phosphatase and protease inhibitors (Roche). 30 μg of proteins were separated by SDS-PAGE electrophoresis on 15 % acrylamide gels ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 10 mM Tris-HCl pH 7.4) containing complete protease inhibitor cocktail (Roche) followed by two rounds of differential centrifugation ...
-
bioRxiv - Cell Biology 2024Quote: ... 6mM Tris HCl pH 6.8) supplemented with EDTA-free protease inhibitor cocktail (Roche). Tissues were disrupted using potter homogenizer (2x 25 strokes at 2000rpm) ...
-
bioRxiv - Cancer Biology 2024Quote: ... 25 mM Tris HCl pH 7.4) supplemented with protease and phosphatase inhibitors (Roche). Proteins were then denaturated in Laemmli buffer (Bio-Rad ...
-
bioRxiv - Genetics 2023Quote: ... 5 mM Tris-HCl (pH 7.4) supplemented with Protease Inhibitor cocktail (Roche, #04693159001) and transferred to a 7 mL Dounce tissue grinder (KIMBLE KONTES ...
-
bioRxiv - Neuroscience 2023Quote: ... 50 mM Tris-HCl (pH 8.0) with protease and phosphatase inhibitors (1X Roche Complete Mini ...
-
bioRxiv - Neuroscience 2023Quote: ... 50 mM Tris-HCl (pH 8.0) with protease and phosphatase inhibitors (1X Roche Complete Mini ...
-
bioRxiv - Cell Biology 2022Quote: ... 50 mM Tris HCL pH 7.5) supplemented with cOmplete protease inhibitor by Roche. The GFP-tagged proteins were targeted using 10 µL GFP-Trap magnetic beads (Chromotek ...
-
bioRxiv - Neuroscience 2022Quote: ... were transfected into HEK293 cells at a ratio of 3:3:1 (hGluN1/hGluN2A/EGFP) using X-tremegene HP (Roche) at a 1:100 ratio with optiMEM ...
-
bioRxiv - Cell Biology 2023Quote: ... 0.5% Zwittergent 3-12 (N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate) and protease inhibitor (Complete, Roche Applied Science)) for 2 h at 0°C (ref A) ...
-
bioRxiv - Biochemistry 2020Quote: ... Trypsin-6 (11418025001; Roche), Trypsin-7 (90057 ...
-
bioRxiv - Biophysics 2020Quote: ... using FUGENE 6 (Roche) and grown in IMDM medium with 10% FBS ...
-
bioRxiv - Biophysics 2020Quote: ... using FUGENE 6 (Roche) and grown in IMDM medium with 10% FBS ...
-
bioRxiv - Immunology 2020Quote: ... using FUGENE 6 (Roche) and grown in IMDM medium with 10% FBS ...
-
bioRxiv - Immunology 2020Quote: ... using FUGENE 6 (Roche) and grown in IMDM medium with 10% FBS ...