Labshake search
Citations for Roche :
5701 - 5750 of 6565 citations for Estrone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 2008) using commercial transcription kits and RNA labeling mix (REF #: 11277073910, Lot #: 26591021 & REF #11426346910:, Lot # 24158720; Roche Applied Science) to generate digoxigenin-labeled and fluorescein-labeled riboprobes using a plasmid template containing cDNA ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... Ten million cells were then lysed with a Dounce homogenizer on ice in the kit lysis buffer supplemented with EDTA-free protease inhibitors (Roche). Cell homogenates were subjected to immunoprecipitation using magnetic beads-coupled anti-TOMM22 antibodies ...
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bioRxiv - Plant Biology 2023Quote: The YFP digoxygenin-labelled antisense and sense RNA probes were generated by in vitro transcription according to the instructions provided with the DIG RNA labelling kit (SP6/T7; Roche) using a plasmid containing the YFP sequence as template ...
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bioRxiv - Plant Biology 2023Quote: ... We used the SYBR Premix Ex Taq II reagent kit (Vazyme, Q311-02) to perform qRT-PCR on a LightCycler 96 Real-Time PCR System (Roche). Rice OsACTIN1 was used as the internal reference ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was carried out using the KAPA HiFi Hot Start Ready Mix PCR Kit (Cat# KK2602, KAPA Biosystems, Wilmington, MA). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... Antisense and sense RNA probes were synthesized from the linearized plasmid template by in vitro transcription using a DIG RNA Labeling Kit (Roche). In situ hybridization was performed on cryo-sections of telencephalon as previously described (Masai et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Libraries were base-pair selected based on Agilent 2100 Bioanalyzer profiles and normalized determined by KAPA Library Quantification Kit (Roche). The libraries were built from 3 to 5 different mice per genotype ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA (150-200 ng) was sheared and HiSeq sequencing libraries were prepared using the KAPA Hyper Prep Kit (Roche). Libraries were amplified by PCR (10 cycles) ...
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bioRxiv - Microbiology 2022Quote: A fraction of each RNA sample analyzed by RNAseq was reverse-transcribed to cDNA using Transcriptor First Strand cDNA Synthesis Kit (Roche) and random hexamer primers ...
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bioRxiv - Cancer Biology 2022Quote: ... Between 50 and 100 ng of RNA was used as input for the KAPA RNA HyperPrep Kit with RiboErase (Human/Mouse/Rat) library preparation (Roche) on an automated liquid handling platform (Beckman Coulter) ...
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bioRxiv - Cancer Biology 2022Quote: ... In all available cases staining was performed on the Benchmark ultra-automated Stainer (Ventana) using diamino-benzidine as chromogen (DAB detection kit; Ventana, Roche), and anti-AGR2 monoclonal antibody (1:100 ...
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bioRxiv - Genomics 2022Quote: ... Pooled library (18 total – nine from DNA and 9 from total RNA – see below) concentration was determined using a KAPA library quantification kit (Roche). Libraries were sequenced on a Illumina NextSeq 500 instrument (2×150bp paired-end reads ...
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bioRxiv - Microbiology 2022Quote: ... [30] The RNA was then extracted with the MagNA Pure 96 DNA and Viral NA Small volume kit (06 543 588 001, Roche) on the MagNA Pure 96 Instrument (06541089001 ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting RNA was used as input into library preparation at the first strand synthesis step using the KAPA RNA Hyper Prep Kit (Roche). Libraries were sequenced to a depth of 20 million paired-end 2×150bp reads each on the NovaSeq 6000 (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... and visualized with anti-DIG-AP antibody according to the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche) instructions on a Chemidoc MP.
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bioRxiv - Cancer Biology 2022Quote: ... followed by DNA purification and amplification for the library with index primers using KAPA HiFi HotStart Kit (Roche, Indianapolis, IN). The libraries were purified ...
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bioRxiv - Cell Biology 2022Quote: ... RNA-Seq library was prepared according to KAPA mRNA HyperPrep kit with 200-300 bp insert size (Roche, Wilmington, MA) using 250 ng of total RNAs as input ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic PCR for ATP13A2 floxed or KO alleles was performed using 100 ng genomic DNA and the Kapa2g Fast HotStart PCR Kit (Roche). PCR primers included a forward primer (5’-CTGCAGCTTCGAGAGGAAAG-3’) ...
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bioRxiv - Biochemistry 2023Quote: ... was used for cDNA synthesis and RT-qPCR was performed using KAPA SYBR FAST qRT PCR kit (KK4602, KAPA Biosystems). Taf10 was used as a control for normalisation and the fold change in mRNA levels were calculated by 2^-ddct method.
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bioRxiv - Plant Biology 2024Quote: ... Post-hybridization washes and immuno-chemiluminescent detection of the bound probe were conducted following the instructions provided in the DIG Northern Starter Kit manual (Roche). To assess siRNA size via northern blot analysis ...
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bioRxiv - Microbiology 2024Quote: ... and 1 μL of the reverse transcrip-tion mix from the Titan One Tube RT-PCR System kit (Roche, Switzerland) was added ...
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bioRxiv - Microbiology 2024Quote: ... and 100 ng were used for paired-end Illumina sequencing library preparation using the KAPA HyperPlus Library Preparation Kit (Roche) and the KAPA Unique Dual-Indexed Adapater Kit (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR was performed using the Luna Universal One-Step RT-qPCR Kit (Biolaps) according to the manufacturer protocol on a LightCycler real-time PCR thermocycler (Roche). The analysis of the infection rate based on PCR data was done with the binGroup package [42] in R software.
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bioRxiv - Microbiology 2024Quote: ... Plate-based DNA library preparation for Illumina sequencing was performed on the PerkinElmer Sciclone NGS robotic liquid handling system using Kapa Biosystems library preparation kit (Roche). 200 ng of sample DNA was sheared to 300 bp or 600 bp using a Covaris LE220 focused-ultrasonicator ...
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bioRxiv - Molecular Biology 2024Quote: ... To detect for DNA containing complementary sequences on membrane-bound DNA a α-32P-dCTP-labelled probe spanning the region of 37-611 nts on human mtDNA was synthesized using High Prime DNA Labeling Kit (Roche). After pre-hybridizing the membrane with Church’s buffer (250 mM NaPi pH 7.2 ...
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bioRxiv - Neuroscience 2024Quote: ... RNAseq libraries were prepared by KAPA Stranded mRNA Sample Preparation Kit according to the manufacturer’s protocol (Kapa Biosystems, MA, USA) as previously described (Grabowska et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... A first PCR reaction was prepared from genomic DNA using the KAPA HiFi HotStart PCR Kit (KAPA Biosystems/Roche Sequencing) in a total reaction volume of 20 µL ...
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bioRxiv - Molecular Biology 2024Quote: ... A first PCR reaction was prepared from genomic DNA using the KAPA HiFi HotStart PCR Kit (KAPA Biosystems/Roche Sequencing) in a total reaction volume of 20 µL ...
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bioRxiv - Microbiology 2024Quote: ... Different dilutions of the enzyme preparation were assayed for 1 hour and analyzed with a luciferin-luciferase coupled reaction (76) using the CLSII-Bioluminiscent ATP Assay Kit (Roche).
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bioRxiv - Cancer Biology 2024Quote: ... Gene expression was detected using the LightCycler 480 SYBR Green I Master Mix or Probes Master Mix kit (Roche Diagnostics) after PCR (5 min at 95 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... The size and concentration of each sample’s library were determined using Agilent 2200 Tapestation and KAPA library quantification kit (Roche, #KK4824) respectively ...
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bioRxiv - Developmental Biology 2024Quote: RNA-seq libraries were constructed by the ICM Platform (PARIS) from 100 ng of total RNA using the KAPA mRNA Hyperprep kit (Roche) that allows to obtain stranded polyA libraries ...
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bioRxiv - Plant Biology 2023Quote: Quantitative real-time PCR (qRT-PCR) analysis was performed using the KAPA SYBR FAST qPCR kit (KAPA Biosystems Inc., Switzerland) to check the gene expression levels of GLXI;1 ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA (1 μg) was reverse transcribed to cDNA using the Transcriptor First Strand cDNA Synthesis Kit (catalog no. 04379012001, Roche), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Libraries were quantified by real-time PCR using the KAPA Library Quantification Kit for Illumina Sequencing Platforms (Roche, Basel, Switzerland), followed by quality control using a DNA 100 Chip on a Bioanalyzer 2100 ...
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bioRxiv - Cell Biology 2024Quote: ... total RNA was extracted from 200 µL of indicated cell supernatants using a High Pure Viral RNA Kit (Roche, Germany). The extracted RNA was eluted in 50 µL of elution buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The short insert paired-end libraries for the whole genome sequencing were prepared with PCR free protocol using KAPA HyperPrep kit (Roche). The library was sequenced on NovaSeq 6000 (Illumina ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and library preparation was carried out using the Accel-NGS 2S Plus DNA Library Kit (Swift Bioscience) and amplified with the KAPA HiFi DNA polymerase (Roche). The amplified library was sequenced on NovaSeq 6000 (Illumina ...
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was performed using 4µl of cellular RNA and Taqman RNA-to-Ct 1step kit (Thermo Fischer 4392938) following manufacturer recommendations using a LightCycler 480 (Roche). For IFN-I and ISG expression analysis ...
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bioRxiv - Genetics 2024Quote: ... In order to link the sequencing adaptors and double indexed libraries the second PCR step were performed using TG Nextera® XT Index Kit (FC-131-200(1,2,3) and the Kappa Hifi Hot Start (Roche KK2602) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was obtained by automated purification using the MagNA Pure 96 DNA and Viral NA Small Volume Kit and DNA Blood ds SV 2.0 protocol (Roche Diagnostics). The DNA was quantified with Qubit fluorometer (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... One hundred ng of genomic DNA was used for PCR amplification of the target loci using KAPA HiFi HotStart PCR kit (Roche) and the primer sets in Table S1 ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 500 ng of RNA were then retrotranscribed using the Transcriptor First Strand cDNA Synthesis kit (Roche LifeScience) as previously described (Montañés et al. ...
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bioRxiv - Cancer Biology 2024Quote: The Terminal dUTP Nick End Labeling (TUNEL) and In Situ Cell Death Detection Kits (TMR Red #12156792910; Roche Applied Science) were used to examine apoptosis ...
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bioRxiv - Cancer Biology 2024Quote: ... 1μg of total RNA was reverse transcribed using the Transcriptor First Strand cDNA Synthesis Kit (Roche Life Sciences, Penzberg, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... as in viral infection.9 TFIIB knockdown libraries were prepped with KAPA Stranded RNA-seq Kit with RiboErase (HMR) (Roche) using 500 ng of input RNA and amplified for 9 cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA-seq libraries were prepared using 500 ng of total RNA by using KAPA stranded RNA-seq kit for Illumina (Roche) as per manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by primary antibody labelling (16-120 min) and signal detection carried out with the OptiView DAB IHC Detection Kit (VENTANA/Roche), as described previously (33) ...
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bioRxiv - Plant Biology 2023Quote: ... Paromomycin probe was labeled with dUTP-DIG during PCR reaction (PCR DIG probe synthesis kit, Roche; see table primers list). Membrane was pre-hybridized 1h at 50°C in a DIG eazy Hyb (Roche ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and an additional step 72°C for 10 min performed on a Light Cycler 480 with the SYBR Green I Master kit (Roche) (qPHD UM2/GenomiX Platform ...