Labshake search
Citations for Roche :
5751 - 5800 of 6565 citations for Estrone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The library concentrations were also assessed by qPCR using the KAPA Library Quantification Kit (Complete, Universal; Kapa Biosystems, Woburn, MA).
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bioRxiv - Evolutionary Biology 2023Quote: Antisense probes were synthesized using purified PCR products (1 μg per reaction) (supplementary table S16) and DIG RNA labeling Kit (T7) (Roche), following the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: Viral RNA was extracted from 100 µL of tissue or nasal wash samples using MagNA Pure 24 Total NA Isolation Kit on the MagNA Pure 24 system (Roche) and eluted with 50 µL of water ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real time PCRs (qPCRs) were performed in a total volume of 10μl with Kapa SYBR Fast qPCR kit (KAPA Biosystems) on a QuantStudio 3 Real-Time PCR system ...
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bioRxiv - Immunology 2023Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Developmental Biology 2023Quote: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed using the In Situ Cell Death Detection Kit (11684795910, Roche) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed using a FastStart Universal Probe Master (Rox) kit (Roche Applied Science) or Luna Universal Probe qPCR Master Mix (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... labeled antisense riboprobes were generated from the cDNA template using T7 RNA polymerase and a DIG-UTP RNA labeling kit (Roche). Brain sections were fixed in 3% phosphate buffered paraformaldehyde for 5 min followed by a rinse in phosphate buffered saline ...
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bioRxiv - Neuroscience 2023Quote: ... the cDNA was amplified using KAPA SYBR fast qPCR kit (Nippon genetics) with LightCycler 480 II system (Roche, Mannheim, Germany). The temperature profile of the reaction was 95 °C for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... Afterwards 5ng DNA fragments were pooled and proceeded on with library construction using KAPA Hyper Prep Kit (Kapa Biosystems #KK8504) according to the standard protocol ...
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bioRxiv - Immunology 2023Quote: ... lactate dehydrogenase (LDH) was measured in supernatants collected at 48 hours post transfection using cytotoxicity detection kit PLUS Assay (CYTODET-RO, Roche) according to manufacturer’s instructions and plates were read at 490 nm.
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bioRxiv - Molecular Biology 2023Quote: 100 pmol gapmers (Supplemental Table S4) were labeled with biotin-16-ddUTP (Jena) and a 2nd generation DIG-oligonucleotide 3’end-labeling kit (Roche) using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer ...
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bioRxiv - Microbiology 2023Quote: ... qPCR analysis was done on 100ng of DNA using a LightCycler 480 Probe Master-Mix kit (Roche, Cat. No. 04707494001) and a primer and probe set specific to MHV68 gB to quantify the number of viral genome copies ...
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bioRxiv - Pathology 2023Quote: Total RNA was extracted from sigmoid colon tissue using a High Pure RNA Isolation kit (Roche Applied Science, Penzberg, Germany) according to the manufacturer’s instructions ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... and in vitro transcribed using T7 (pSC-pbx3a, pSC-cdx1b) or T3 polymerase (pSC-best4, pSC-otop2) and fluorescein or digoxygenin RNA labeling kits (digoxygenin: Roche, Cat# 11277073910 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We used a Covaris E220 sonicator to shear DNA and we then prepared genomic libraries using the KAPA HyperPlus kit (Roche). Individual libraries were indexed using KAPA UDI’s and pooled libraries were sent to Novogene for Illumina paired-end 150 bp sequencing on a Novaseq X.
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bioRxiv - Neuroscience 2023Quote: Apoptotic cell death was assessed as formation of fragmented nucleosomes using the Cell Death Detection ELISAPlus kit (Roche Applied Science) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... ChIP-seq libraries were prepared by Duke University Sequencing and Genomic Technologies Core Facility (Duke Sequencing Facility) from 1 ng of ChIP DNA using the KAPA HyperPrep Library Kit (Roche) to generate Illumina-compatible libraries ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was used to generate the sequencing libraries using the KAPA Stranded mRNA Seq kit for the Illumina sequencing platform according to the manufacturer’s protocol (KAPA biosystems). Libraries were sequenced on an Illumina NextSeq 550 using 150 nt paired-end sequencing ...
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bioRxiv - Developmental Biology 2023Quote: ... Afterwards 5ng DNA fragments were pooled and proceeded on with library construction using KAPA Hyper Prep Kit (Kapa Biosystems #KK8504) according to the standard protocol ...
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bioRxiv - Cell Biology 2023Quote: Apoptotic cells were identified by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining using the In Situ Cell Death Detection Kit (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Illumina sequencing library was prepared using a TruSeq-based protocol (KAPA LTP Library Preparation kit Illumina, catalog num. 7961898001, Roche) and manufacturer protocol was strictly followed ...
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bioRxiv - Immunology 2023Quote: ... cytotoxicity was determined based on the release of lactate dehydrogenase (LDH) into the co-culture supernatant measured by Cytotoxicity Detection Kit (Roche) and using a Synergy 2 Multi-Mode Microplate Reader (Biotek ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were constructed using ∼50 ng of mRNA using the Kapa Stranded RNA-Seq Library Preparation Kit (KAPA Biosystems, Illumina). Indexes were designed according to TruSeq RNA UD specifications (#20020591 ...
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bioRxiv - Genomics 2023Quote: ... All strains were sequenced using an Illumina MiSeq platform and the KAPA LTP library preparation kit (KAPA Biosystems, Wilmington, MA). The pooled libraries were loaded into a MiSeq system and sequenced using a MiSeq reagent kit v2 with 2 × 250 cycles (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... and nucleic acid extracted using the MagNA 96 instrument with DNA/Viral NA small volume kits (Roche, Laval, Quebec, Canada). Ophidiomyces ophidiicola was not detected in these samples by qPCR (Allender et al ...
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bioRxiv - Evolutionary Biology 2023Quote: ... present-day mitochondrial genome was amplified for baits in four overlapping fragments using the Expand Long Range dNTPack kit (Roche) with four selected primers pairs (175F-5151R ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real-time PCR was performed in triplets for each sample using the LightCycler DNA Master SYBR Green I Kit in a LightCycler 480 System (Roche). The relative copy number of Gapdh RNA was used for normalization ...
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bioRxiv - Biochemistry 2023Quote: Cytotoxicity assays were performed as described29 (with minor changes. Proliferation of human cells was assessed using an MTT colorimetric assay (Cell Proliferation Kit I, Roche). HeLa (epithelial cells ...
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bioRxiv - Cancer Biology 2023Quote: ... The cDNAs were labeled with [α32P]-dCTP using the Random Primed DNA Labeling Kit following the protocol provided by Roche. Total RNA was TRizol (Invitrogen ...
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Remodeling of the Ribosomal Quality Control and Protein Translation by a Viral Ubiquitin DeconjugasebioRxiv - Cell Biology 2023Quote: ... One μg total RNA was reverse transcribed using the SuperScript VILO cDNA Synthesis kit and quantified using a LightCycler 1.2 instrument (Roche Diagnostic) with the LC FastStart DNA master SYBR green I kit and specific primers (Supplementary Table S3) ...
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bioRxiv - Developmental Biology 2023Quote: Apoptotic cells present in gonadal histological preparations were revealed by the terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick end labelling (TUNEL) assay using the Fluorescent In Situ Cell Death Detection Kit (Roche ref ...
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bioRxiv - Genomics 2022Quote: ... RNA-seq libraries were prepared using 1 μg of total RNA input using KAPA stranded mRNA-seq kit for Illumina (Roche) as per manufacturer’s instruction and paired-end sequenced on NovaSeq 6000 (Illumina).
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bioRxiv - Developmental Biology 2022Quote: ... TUNEL staining on 60 dpf paraffin embedded testes sections were performed using a fluorescein In Situ Cell Death Detection Kit (11684795910, Roche). Immunolabelling with Vasa antibody was done before the TUNEL staining ...
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bioRxiv - Developmental Biology 2023Quote: ... Digoxigenin (DIG)-labeled sense and antisense riboprobes were prepared using the in vitro transcription DIG labeling kit (Roche, IN, USA). Hybridization was carried out at 600C overnight and the slides were washed and probed with an alkaline phosphatase-conjugated anti-DIG antibody (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was done following manufacturer’ s instruction (In Situ cell Death Detection Kit TMR red, Roche).
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bioRxiv - Genomics 2023Quote: ... 1ng of Cut and Run DNA was used to make libraries using the KAPA Hyper prep kit and SeqCap adapters A &B (Roche) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The 12 and 32 libraries were equimolarly pooled and subjected to qPCR quantification using the KAPA library quantification kit (Roche). Sequencing was carried out on a NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... rRNA-depleted RNA-seq libraries were then amplified in a BioRad CFX96 using the Kapa HiFi Real-Time Amplification Kit (Roche) in a 50 μL reaction with 25 μL master mix ...
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bioRxiv - Developmental Biology 2023Quote: RNA sequencing (RNAseq) libraries were prepared using 1 µg of total RNA using the KAPA stranded mRNA-seq kit (Roche) according to the manufacturer’s specifications ...
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bioRxiv - Cancer Biology 2022Quote: ... or IRF4-R98AC99A variants was processed by use of the KAPA mRNA Hyper Prep Kit for Illumina Platforms (KK8580; Roche) and KAPA Single-Indexed Adapter Kit (KK8700 ...
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bioRxiv - Systems Biology 2023Quote: ... Paired-end RNA sequencing libraries were prepared by using a KAPA RNA HyperPrep kit from Kapa Biosystems (Wilmington, MA, USA) as described previously33 ...
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bioRxiv - Immunology 2023Quote: ... Lactate dehydrogenase (LDH) in the supernatants from either cell type was quantified using the LDH Cytotoxicity Detection kit (TaKaRa or Roche) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: RNA was extracted at 120 h or 144 h post aggregation using the High Pure RNA Isolation Kit (Roche 11828665001) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: The library was prepared by the Dana Farber Next Generation Sequencing Core Facility using a KAPA library quantification kit (Roche). Sequencing was done on an Illumina NovaSeq 6000 with total RNA.
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bioRxiv - Neuroscience 2023Quote: ... RNA-seq libraries were prepared by the Van Andel Genomics Core from total RNA using the KAPA RNA HyperPrep Kit with RiboseErase (v1.16) (Kapa Biosystems). Base calling was done by Illumina NextSeq Control Software (NCS ...
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bioRxiv - Neuroscience 2023Quote: The TUNEL assay was caried out using the In Situ Cell Death Detection TMR rot kit (Roche, cat. no. 12156792910) according to the manufacturers protocol ...
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bioRxiv - Neuroscience 2023Quote: ... RNA sequencing libraries were prepared with 200 ng of total RNA using KAPA RiboErase Stranded RNA-Seq kit (cat # KK8483) following standard protocol (Roche). Sequencing was done at Stanford Functional Genomics Facility (Stanford ...
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bioRxiv - Neuroscience 2023Quote: ... or Ultra View Universal Alkaline Phosphatase Red Detection Kit (yielding pink signals) using standard machine settings (all solutions were from Ventana, Roche). Counterstaining (light blue background ...
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bioRxiv - Systems Biology 2023Quote: ... the entire eluate was used as input for a 300 μl PCR reaction mastermix with the KAPA HiFi HotStart DNA Polymerase kit (Roche Sequencing Solutions Inc ...