Labshake search
Citations for Roche :
5501 - 5550 of 6565 citations for Estrone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... first strand cDNA synthesis was performed using mRNA with an oligo-dT primer using Transcriptor High Fidelity cDNA Synthesis Kit (Roche), according to the manufacturer′s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The amplified fragments were then used to produce digoxigenin (DIG)-labeled sense and antisense RNA probes using a DIG RNA Labeling Kit (Roche). Whole-mount in situ hybridization was performed following (39) ...
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bioRxiv - Microbiology 2020Quote: ... gRNA encoding DNA sequences were amplified in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems) and sequenced on an Illumina NextSeq (High Output ...
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bioRxiv - Plant Biology 2021Quote: ... Gene transcription was quantified by qPCR using a LightCycler 480 SYBR Green I Master kit and LightCycler 480 (Roche, Switzerland). The PCR conditions were 95 °C for 10 min followed by 45 cycles of 95 °C for 10 s ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was reverse-transcribed to cDNA using a Transcriptor First Strand cDNA Synthesis kit (Roche Diagnostics BV, Almere, Netherlands). Viral genomic RNA was quantified by real-time quantitative RT-PCR specific for the RdRp gene of SARS-CoV-2 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Library-quality was assessed using Fragment Analyzer and libraries were quantified by qPCR using the Kapa Library Quantification Kit (Roche). Sequencing was performed on a NovaSeq S4 lane (Illumina ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 1 μg of total RNA was reverse-transcribed with oligo dT primers using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche) according to the manufacturer’s protocols ...
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bioRxiv - Genomics 2021Quote: ... RNA-seq libraries were constructed with removal of ribosomal RNA using the KAPA Stranded RNA-Seq kit with RiboErase (Roche). The RNA extraction and library preparation were performed by the same technician to minimize confounding effects ...
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bioRxiv - Microbiology 2020Quote: ... previously published assay (“one-step real-time RT-PCR E assay”) using the One-Step RT-qPCR kit (Roche Diagnostics). For each run ...
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bioRxiv - Microbiology 2019Quote: ... Sendai Virus (SeV) and Modified vaccinia Ankara (MVA)-gfp viral stocks were extracted using the High Pure Viral Nucleic Acid Kit (Roche). When indicated ...
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bioRxiv - Molecular Biology 2021Quote: Serum creatinine was determined using the creatininase/creatinase specific enzymatic method described by Bömer using a commercial kit (Alpha Laboratories Ltd. Eastleigh, UK) adapted for use on a Cobas Fara centrifugal analyser (Roche Diagnostics Ltd ...
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bioRxiv - Microbiology 2021Quote: Total nucleic acid was extracted from 253 resuspended vaginal swab samples using the MagNA Pure Compact Nucleic Acid Isolation Kit (Roche) [33] ...
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bioRxiv - Plant Biology 2021Quote: ... Purified DNA amplicons with flanking T7 and SP6 promoters were used for in vitro synthesis of digoxigenin (DIG)-UTP-labelled single-stranded RNA sense and anti-sense probes by using T7 and SP6 polymerases from a DIG RNA Labelling Kit (Roche). The non-complementary sense probe was used as negative control for each gene ...
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bioRxiv - Microbiology 2021Quote: ... Library preparation and sequencing data processing were performed according to Nilsen et al (2020) except the library was quantified with the KAPA library quantification kit (universal; Roche) in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... RNA was isolated anew from stored clinical samples (naso- and/or oropharyngeal swabs in GLY-medium) using the MagNA Pure 96 DNA and Viral NA Small Volume Kit (Roche) and was assessed with a single replicate to obtain a first indication of clinical specificity and sensitivity ...
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bioRxiv - Microbiology 2020Quote: ... the DNA was subjected to pre-hybridization and hybridization using a DIG High Prime DNA labeling and detection starter kit II (Roche) according to the manufacturer’s instructions with a DIG-labeled probe of ∼100-200 bp annealing in the targeted gene (for primer sequences ...
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bioRxiv - Molecular Biology 2021Quote: ... DAB staining was performed using a Ventana Ultraview DAB detection kit in a Ventana BenchMark XT processor (Ventana Medical Systems, a division of Roche). In addition ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNAse was inactivated with 1 µl of Stop Solution at 65°C for 10 min and RT was performed using Transcriptor First Strand cDNA Synthesis Kit (Ref. 04 897 030 001, Roche) in a final volume of 20 µl ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription was performed using 200-500 ng of total RNA in the Transcriptor First Strand cDNA synthesis kit (Roche) with 60 μM random hexamer and 6.25 μM oligo(dT)18 primer ...
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... rRNA-depleted RNA-seq libraries were prepared from 100-1,000ng RNA per sample using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche, #KK8560). RNA fragmentation was performed at 94°C for 6 minutes and 10 PCR cycles were used during library amplification with TruSeq single-index adapters (Illumina ...
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bioRxiv - Genomics 2020Quote: ... cDNAs were prepared using Superscript II reverse transcriptase (Life Sciences) using hexamer random primers and qPCR was performed using the SybrGreen aMster kit (Roche) on a LightCycler 480 II ...
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bioRxiv - Cell Biology 2020Quote: ... and RNAseq libraries (2 biological replicates per condition comprised of a pool of 4 PA-gel cultures each) prepared using KAPA mRNA HyperPrep Kit (Roche) and IDT dual indexed sequencing adaptors ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was performed with Kapa SYBR Fast qPCR Kit Master Mix (2×) Universal (Kapa Biosystems Ltd., Wilmington, MA, USA) on a CFX connect real-time system (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2020Quote: RNA was purified from ∼50 mg of homogenized tissue from all organs by using the MagNAPure 96 system and the MagNA Pure 96 DNA and Viral NA Large Volume Kit (Roche) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... RNA samples with a RIN of at least 7 were used to generate libraries by GTAC using the RNA zero kit (Roche). Samples were prepared according to library kit manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting amplified and barcoded libraries were then equimolarly pooled and quantified using a qPCR method (KAPA library quantification kit, Roche). The sequencing was carried out using single-read mode (SR100 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Detection of apoptotic cells in tissue sections was performed by the TUNEL assay with the In-Situ Cell Death Detection Kit (TMR red, Roche), according to the protocol described previously22 ...
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Population structure and inbreeding in wild house mice (Mus musculus) at different geographic scalesbioRxiv - Genetics 2022Quote: ... MA).The UNC High Throughput Sequencing Facility generated sequencing libraries using Kapa DNA Library Preparation Kits (Kapa Biosystems, Wilmington, MA). Each library was run on its own lane of a HiSeq4000 (Illumina Inc ...
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bioRxiv - Cell Biology 2022Quote: mRNA was purified from ∼200 ng of total RNA with oligo-dT beads and sheared by incubation at 94°C in the presence of Mg2+ with the KAPA mRNA HyperPrep kit (Roche). Following first-strand synthesis with random primers ...
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bioRxiv - Plant Biology 2022Quote: ... RNA libraries for sequencing were prepared at the Functional Genomics Laboratory at UC Berkeley using a KAPA RNA HyperPrep Kit (Roche) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... RNA-seq libraries of template molecules suitable for high throughput sequencing were established from 300ng of total RNA using the KAPA RNA HyperPrep Kit after a first step of purification using poly-T oligo-attached magnetic beads (Roche). Libraries were sequenced on the Illumina Hiseq 4000 sequencer as paired-end 100 base reads ...
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bioRxiv - Developmental Biology 2022Quote: ... Riboprobes labeled with digoxigenin (DIG), fluorescein (FLU) or dinitrophenol (DNP) were prepared as described (Opitz et al., 2011) using DIG RNA labeling kit (Roche), FLU RNA labeling kit (Roche ...
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bioRxiv - Immunology 2022Quote: ... qPCRs were performed using specific primers for each gene (Supplementary Table 2) in Roche LightCycler 480 real-time PCR machine using Lightcycler 480 Sybr Green I Master kit (Roche). Actin was used as a reference gene (Supplementary Figure 1) ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 13 mRNA libraries for Illumina sequencing were prepared using KAPA mRNA HyperPrep Kit (KAPA Biosystems, cat. KK8581) from 250 ng of total RNA ...
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bioRxiv - Cancer Biology 2022Quote: ... Large scale preps of bacteria cultures containing the sgRNA plasmid library were harvested using the Genopure plasmid maxi kit (Roche). SIDP library was packaged in lentiviral particles by large scale co-transfection of HEK293T cells with CELLECTA ready-to-use packaging plasmid (Cellecta – cat.no CPCP-K2A ...
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bioRxiv - Developmental Biology 2022Quote: ... TUNEL staining was performed using ApopTag Red In Situ Apoptosis Detection Kit (S7100, MerckMillipore) combined with peroxidase (POD) coupled anti-DIG antibody (1/200, #11207733910, Roche) followed by tyramide-based amplification61.
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bioRxiv - Microbiology 2022Quote: ... and by quantitative PCR (qPCR) using a KAPA Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems, Roche, Wilmington, MA). Samples were multiplexed and sequenced with a 15% spike-in of PhiX DNA in an Illumina NovaSeq platform (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... and by quantitative PCR (qPCR) using a KAPA Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems, Roche, Wilmington, MA). Samples were multiplexed and sequenced with a 15% spike-in of PhiX DNA in an Illumina NovaSeq platform (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μm sections were stained with H&E and Masson trichrome (Trichrome II Blue staining kit at Nexus special stainer; Roche). Immunohistochemical analysis was performed on cryosections and paraffin sections using a Bench Mark XT automatic staining platform (Ventana ...
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bioRxiv - Genetics 2022Quote: ... were prepared and sequenced by the Van Andel Genomics Core from 500 ng of total RNA using the KAPA mRNA HyperPrep kit (v4.17) (Kapa Biosystems). RNA was sheared to 300-400 bp ...
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bioRxiv - Cancer Biology 2022Quote: ... genomic (g)DNA was extracted both from primary Irf4−/− tumours as well as FACS-sorted control B220+mIgM- BM fr.A-D cells using the High Pure PCR Template Preparation kit from Roche (11796828001). Integrity of resultant gDNA was confirmed in a 2 % Agarose gel ...
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bioRxiv - Molecular Biology 2022Quote: Cytotoxicity was determined as lactate dehydrogenase (LDH) activity in cultured supernatants using a cytotoxicity detection kit (#11644793001; Roche, Mannheim, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... DNase-treated RNA was converted into barcoded polyadenylated mRNA libraries using the Kapa Hyper Stranded mRNA Sample Prep Kit (Roche). Libraries were barcoded with unique dual indexes to prevent index switching and adaptor-ligated double-stranded cDNAs were PCR-amplified for eight cycles with KAPA HiFi DNA Polymerase (Roche) ...
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bioRxiv - Cancer Biology 2022Quote: ... mRNA expression levels were determined by qRT-PCR with KAPA SYBR FAST qPCR Master Mix Kit (Kapa Biosystems, no. KK4610). Relative expression levels were normalized to human ACTB or mouse Actb ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Library quality was assessed using an Advanced Analytical Fragment Analyzer and libraries were quantified by qPCR using the Kapa Library Quantification Kit (Roche). Sequencing has been performed on a NovaSeq6000 S4 lane (Illumina ...
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bioRxiv - Immunology 2022Quote: ... Yield of RNA yield was quantitated by nano-drop and 1 µg of RNA was use to reverse-transcribed to cDNA using the iScript cDNA synthesis kit (BIORAD; #1708891) (Roche). 1:5 diluted cDNAs was used for qPCR by using KAPA SYBR® FAST qPCR Master Mix (5X ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with RCAS-hPDGFB-HA and RCAS-shRNA-p53-Rfp using a Fugene 6 transfection kit (Roche, 11814443001) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability analysis by lactate dehydrogenase (LDH) release assay was performed with the Cytotoxicity Detection Kit (LDH) (Roche, Mannheim, Germany) and has also been described 23,31.
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bioRxiv - Microbiology 2020Quote: ... Library concentrations for pooling of barcoded samples was assessed by RT-qPCR with a KAPA Library Quantification kit (KAPA Biosystems) as recommended for high sensitivity ...
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bioRxiv - Genomics 2021Quote: ... using 400 ng of total RNA from each sample for poly(A) mRNA capture followed by stranded mRNA-seq library construction using KAPA stranded mRNA-seq kit for Illumina (Roche) as per manufacturer’s instruction ...