Labshake search
Citations for Roche :
5851 - 5900 of 6565 citations for Estrone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... RT-PCR was performed using first strand synthesis with specific primers (Reagents) and SYBR FAST qPCR Kit (Kapa Biosystems, Wilmington, MA) in a StepOne cycler (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... Indexed libraries that met appropriate cut-offs for both quantity and quality were quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution was determined with the LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized with 12 µL of RNA and anchored oligo(dT)18 primer in a 20 µL reaction using the Transcriptor First Strand cDNA synthesis kit at 55°C for 30 min for reverse transcriptase reaction (Roche Diagnostics).
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bioRxiv - Microbiology 2020Quote: ... The fragments were treated with end-repair, A-tailing, and ligation of Illumina compatible adapters (IDT, Inc) using the KAPA Illumina Library prep kit (KAPA biosystems). Libraries for the rest of the samples were prepared in 96-well plates ...
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bioRxiv - Developmental Biology 2021Quote: ... Illumina-compatible sequencing libraries were prepared by using a modified version of the KAPA HyperPrep library kit (KAPA BioSystems, Willmington, MA). Quality check steps were performed to assess the fraction of proximally ligated DNA labeled with biotin ...
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bioRxiv - Cell Biology 2020Quote: ... Each RNA-seq library was analyzed by the fragment analyzer (Advanced Analytical Technologies) and quantified by KAPA Library Quantification Kit (KAPA Biosystems) according to the manufacturer’s manual ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative PCR reactions were performed with fast Start Universal Probe Master kit and UPL-probe system in a LightCycler 480 Instrument (Roche Diagnostics) as recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2021Quote: ... with on-column DNase digestion followed by cDNA synthesis using the Transcriptor First Strand cDNA synthesis kit (Roche Molecular Diagnostics, 04379012001) using random primers ...
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bioRxiv - Immunology 2021Quote: Approximately 200-500 ng of FFPE RNA extracted from FFPE slides with a DV200 range between 3-99 or 65-100 ng of fresh frozen RNA (DV200 98-99) per sample were used for RNA library construction using the KAPA RNA Hyper library prep kit (Roche, Switzerland). The number of pre-capture PCR cycles was adjusted based on the quality and quantity of RNA extracted from the samples ...
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bioRxiv - Neuroscience 2020Quote: ... The molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (Roche-KK4824). Individual libraries were normalized to 1.30 nM in preparation for Illumina sequence analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... The library size distribution was checked on an Agilent high-sensitivity DNA chip and the library quantified using the KAPA library quantification kit (KAPA Biosystems). For each reaction ...
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bioRxiv - Genetics 2021Quote: ... PCR products were cleaned using Ampure XP Beads (Agencourt) and quantified using the KAPA Library Quantification Kit for Illumina® Platforms (Roche). Libraries were sequenced on a NextSeq sequencer (Illumina ...
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bioRxiv - Microbiology 2019Quote: ... DNA was extracted and purified with the MagNA Pure LC instrument and the MagNA Pure DNA isolation kit III (Roche Diagnostics), according to the recommendations of the manufacturer ...
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bioRxiv - Developmental Biology 2021Quote: ... and samples with RIN >7 were used for paired-end sequence library construction using the KAPA mRNA HyperPrep Kit (Roche # 08098123702). For ChIP samples ...
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bioRxiv - Developmental Biology 2021Quote: ... mRNA was enriched by poly-A capture and mRNA-seq libraries were generated using KAPA Stranded mRNA-seq Kit (KAPA Biosystems). Libraries were generated from two biological replicates ...
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bioRxiv - Developmental Biology 2021Quote: ... and the whole converted DNA was used as input for library preparation using the Accel-NGS Methyl-Seq DNA Library Kit (Swift Biosciences) according to the recommended protocol and indexing PCR using 2X KAPA HiFi HotStart Uracil+ ReadyMix (KAPA Biosystems). Indexed ...
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR products from each level were pooled in equimolar concentration and the pool was converted into a sequence-ready library with the Kapa Hyper Library Construction kit (Roche, CA) with no PCR amplification ...
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bioRxiv - Molecular Biology 2020Quote: ... The entire ChIP DNA or 10 ng DNA in the case of ChIP input was used to make DNA library with the KAPA Hyper Prep Kit (KAPA Biosystems) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA from OS/PDB tissues and matched healthy bone marrow samples was isolated using the High Pure PCR Template Preparation Kit following the manufacturer’s instructions (Roche Life Science). Genomic DNA from cell lines (MC3T3 and RPE1 ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was synthesized from 1 μg total RNA for each sample using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche, 05081963001), with a mixture of oligo dT and random primers ...
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bioRxiv - Genomics 2020Quote: ... The libraries were visualized with the Fragment Analyzer (Advanced Analytical Technologies) and quantified using the Kapa library quantification kit for Illumina platforms (Kapa Biosystems). [3] Based on the concentration ...
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bioRxiv - Genomics 2019Quote: ... was the substrate for a 10 μL qPCR reaction using either Taq DNA polymerase or KAPA HiFi (KAPA HiFi HotStart PCR kit, Kapa Biosystems) according to the manufacturer’s protocol with barcoded P5 and P7 sequencing primers at 0.5 mM and with 1x EvaGreen dye (Biotium ...
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bioRxiv - Immunology 2021Quote: ... and used as a template for the synthesis of cDNA utilizing the Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland). Total genomic DNA was extracted by boiling BMDCs in a 50 mM NaOH solution as previously described (47) ...
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bioRxiv - Physiology 2019Quote: ... in the lysis buffer (buffer ATL and protinase K) provided in the above DNA isolation kits using a Roche Magnalyser instrument and homogenization tubes containing ceramic beads (Roche, UK). UBR5 PCR primers and pyrosequencing primers were purchased from EpigenDX (Hopkinton ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized with 12 µL of RNA and anchored oligo(dT)18 primer in a 20 µL reaction using the Transcriptor First Strand cDNA synthesis kit at 55°C for 30 min for reverse transcriptase reaction (Roche Diagnostics).
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bioRxiv - Immunology 2020Quote: ... Tagmented DNA was ligated to unique i5/i7 barcoded primer combinations using the Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (2X; KAPA Biosystems) and then purified using AmPure Beads XP (Agencourt) ...
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bioRxiv - Pathology 2019Quote: ... cDNA synthesis was performed with a 1 μg RNA sample using the First Strand cDNA Synthesis kit RT-PCR (Roche, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... 300 ng of total RNA from three biological replicates per B cell subset was used as input for the KAPA stranded mRNA-seq Kit with mRNA capture beads (KAPA Biosystems). Libraries were assessed for quality on a bioanalyzer ...
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bioRxiv - Developmental Biology 2019Quote: ... Sample preparation protocol for the RNASeq libraries was following the manufacturer’s recommendations of KAPA Stranded mRNA-Seq Illumina® Platforms Kit (Roche-Kapa Biosystems). The libraries were sequenced on HiSeq2500 (Illumina ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 0.05% (v/v) Tween-20) and processed through the spin columns from High Pure Viral Nucleic Acid Large Volume kits (Roche, Switzerland). Purified DNA was eluted in either 45 µl of EB buffer (10 mM tris-hydrochloride (pH 8.0 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Total RNA was isolated at day 7 or 14 of MSCs differentiation using the High Pure RNA Isolation kit (Roche Diagnostics). For tissues ...
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bioRxiv - Plant Biology 2019Quote: ... An Illumina DNAseq library was constructed for polishing the PacBio based assembly using 1ug of DNA the same high molecular weight DNA prep with the KAPA HyperPrep Kit (Kapa Biosystems). The Illumina DNAseq library was sequenced on an Illumina HiSeq4000 under paired end mode (150 bp ...
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bioRxiv - Genomics 2019Quote: ... Libraries were quantified for normalization by qPCR using the KAPA Library Quantification Kit for Illumina sequencing platforms (KAPA Biosystems, Boston, MA). The final libraries were also assessed using the DNA1000 kit for the Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 µg of linearized pCR©II-Tc-mef2 or pCR©II-Tc-duf/kirre was in vitro transcribed utilizing the DIG RNA labelling kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... All reverse transcriptase qPCR assays for a given target were performed simultaneously for all samples using the LightCycler Multiplex RNA Virus Master Kit (Roche diagnostics). Each sample was normalized with the geometric mean of the quantification cycle (Cq ...
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bioRxiv - Immunology 2019Quote: ... cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit for library quantitation (Kapa Biosystems) prior to sequencing.
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bioRxiv - Immunology 2019Quote: ... and the quantity and quality of each pool was assessed by the Fragment Analyzer and subsequently by qPCR using the Illumina Library Quantification Kit (Kapa Biosystems) on a Light Cycler LC480II (Roche ...
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bioRxiv - Genomics 2019Quote: ... Quality control for primer target amplification and performance in multiplex PCR was assessed by screening each primer pair in singleplex reactions using gDNA from PC3 or DU145 cells (10 ng) as template (see below) using the HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems). Reactions were supplemented with uracil N-glycosylase (0.2 U ...
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bioRxiv - Cell Biology 2019Quote: ... Libraries were prepared by the Genome Technologies core facility at The Jackson Laboratory using the Ovation RNA-seq System V2 (Nugen) and Hyper Prep Kit (Kapa Biosystems). Briefly ...
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bioRxiv - Genomics 2019Quote: TUNEL staining on rat β-cells was performed 48h after transfection using the TMR red In Situ Cell Death Detection Kit (Roche) combined to polyclonal guinea pig anti-insulin (dilution 1:40 ...
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bioRxiv - Cell Biology 2020Quote: ... Libraries were prepared by the Genome Technologies core facility at The Jackson Laboratory using the Ovation RNA-seq System V2 (NuGEN Technologies) and Hyper Prep Kit (Kapa Biosystems). Libraries were checked for quality and concentration using the D5000 ScreenTape assay (Agilent Technologies ...
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bioRxiv - Microbiology 2019Quote: ... 50 ng of DNA from each sample was pooled with multiplex identifiers for 2-region 454 sequencing on GS FLX Titanium PicoTiterPlates (70675) with the use of a GS FLX Titanium Sequencing Kit XLR70 (Roche Diagnostics). Library construction and 454 pyrosequencing were performed at the National High-Throughput DNA Sequencing Centre ...
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bioRxiv - Molecular Biology 2020Quote: ... A-tailed and ligated to an adaptor in the form of a uracil-containing stem-loop using the KAPA HTP Library Preparation Kit PCR Free (KAPA Biosystems). Lambda exonuclease (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative reverse transcription PCR was performed using Eppendorf Realplex2 Mastercycler with reaction mixtures prepared with KAPA SYBR Fast Universal qPCR Kit (KAPA Biosystems). The PCR primers for KIF1A ...
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bioRxiv - Biochemistry 2019Quote: ... The cDNA was then used to perform qRT PCR using the KAPA SYBR FAST qRT PCR master mix kit (KK4602, KAPA Biosystems) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Poly-A mRNA was purified from total RNA using oligodT magnetic beads and strand-specific indexed libraries were prepared using the KAPA Stranded RNA-Seq kit followed by ten cycles of amplification using KAPA HiFi DNA polymerase (KAPA Biosystems). Libraries were quantified and pooled based on a post-PCR Agilent Bioanalyzer and 75 bp paired-end reads were generated on the Illumina HiSeq v4 following the manufacturer’s standard sequencing protocols (as above).
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bioRxiv - Bioengineering 2021Quote: ... 1μg of total RNAs were used to prepared ribo-minus RNA-seq libraries using the KAPA Stranded RNA-Seq Kit with RiboErase (Roche, KK8484), and the depletion of rRNAs confirmed by fluorometric quantification using the Qubit RNA HS Assay kit (Invitrogen) ...
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bioRxiv - Genomics 2021Quote: ... target sites were amplified directly from genomic DNA in the lysate using a KAPA HiFi HotStart PCR kit (KAPA Biosystems, KK2501) as previously described18 ...
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bioRxiv - Genomics 2021Quote: ... 1-8 ng of ChIPed DNA was treated with end-repair, A-tailing, and ligation of Illumina compatible adapters (IDT, Inc) using the KAPA-Illumina library creation kit (KAPA biosystems). The ligated products were enriched with 8-10 cycles of PCR (HiFi premix ...
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bioRxiv - Genomics 2021Quote: Genomic DNA libraries were constructed for high throughput sequencing using the KAPA hyper Prep Library Preparation Kit (Kapa Biosystems, Woburn, MA). Mechanical DNA shearing was performed with the Covaris S220 through microTube-50 AFA Fiber Screw-Cap (Covaris® ...