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Citations for Roche :
5451 - 5500 of 6565 citations for Estrone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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PHF2 regulates homology-directed DNA repair by controlling the resection of DNA double strand breaksbioRxiv - Molecular Biology 2019Quote: ... cDNA synthesis and PCR amplification were carried out in the same tube using the qScript One-Step SYBR Green qRT-PCR Kit (Quantabio) and a LightCycler480 II (Roche). All reactions were performed in triplicate ...
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bioRxiv - Plant Biology 2019Quote: ... with 340bp insert size according to KAPA Library Preparation Kit with no PCR Library Amplification/Illumina series (Roche-Kapa Biosystems) protocol and sequenced on HiSeq2000 (v4 ...
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bioRxiv - Plant Biology 2019Quote: ... with 340bp insert size according to KAPA Library Preparation Kit with no PCR Library Amplification/Illumina series (Roche-Kapa Biosystems) protocol and sequenced on HiSeq2000 (v4 ...
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bioRxiv - Bioengineering 2019Quote: ... For molecular cloning 3 µg of RNA were reverse transcribed for 30 min at 50°C using an oligo(dT) primer and the Transcriptor High Fidelity cDNA Synthesis Kit (Roche) followed by 5 min enzyme inactivation at 85°C according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: The labelling of the probes was carried out according to the instruction manual of the Dig DNA labelling kit (Roche). In situ hybridization was performed as described previously63 ...
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bioRxiv - Microbiology 2019Quote: ... The cytotoxic potential of the samples was determined from THP1-cell supernatants after 3 hours by using the Cytotoxicity Detection Kit (Roche) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... Extracted RNA samples with RIN >7 underwent library preparation for polyA tailed mRNA with a RNA concentration of >1ng/1ul using with Kapa RNA hyperPrep kit (Roche), followed by 75 cycles of Next-generation sequencing with NextSeq® (Illumina® ...
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bioRxiv - Cancer Biology 2019Quote: ... All samples were individually barcoded and quantitated with the KAPA Library Quantitation Kit for Illumina Platforms (Kapa Biosystems, Wilmington, MA) in conjunction with an Agilent Bioanalyzer DNA 1000 analysis (Agilent Technologies ...
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bioRxiv - Microbiology 2019Quote: ... PCR amplicons were purified using the High Pure PCR Product Purification Kit of ROCHE™ (Roche Diagnostics Gmb, Mannheim, Germany). Sequencing was performed using MiSeq™ Illumina® (2 x 300 bp ...
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bioRxiv - Developmental Biology 2020Quote: ... The RNA concentration was measured by a spectrophotometer (NanDrop1000; Thermo) followed by a reverse transcription process using PrimeScript RT reagent kit (Roche). Real time PCR analysis was performed using SYBR green mix (Roche ...
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bioRxiv - Genomics 2019Quote: ... Library quality was assessed using a Fragment Analyzer (Advanced Analytical Technologies, Inc., Iowa, USA) and libraries were quantified by qPCR using the Kapa Library Quantification Kit (Roche). Sequencing was performed on a NovaSeq (Illumina ...
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bioRxiv - Developmental Biology 2019Quote: TUNEL assay for scoring apoptotic cells was performed using In situ cell death detection kit fluorescein (Roche, Catalog No.11684795910), as per manufacturer’s protocol.
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bioRxiv - Molecular Biology 2019Quote: ... 6-10ng of immunoprecipitated material was used for ChIP-seq library preparation using the KAPA Hyper prep kit (KAPA Biosystems). Libraries were sequenced on an Illumina HiSeq 2500 platform on SE50 mode.
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bioRxiv - Immunology 2019Quote: PFA fixed zebrafish larvae were used in combination with the In situ Cell Death Detection Kit (TMR and Fluorescein) (Roche). After O/N fixation larvae were washed and permeabilized for 45 min at 37°C using Proteinase K ...
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bioRxiv - Physiology 2019Quote: ... Total RNA was reverse-transcribed to complementary DNA (cDNA) using the Transcriptor First Strand cDNA Synthesis Kit (Roche, Indianapolis, IN). RT-PCR amplification was consisted of an initial denaturation step (95°C for 10 min) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Paraffin was dissolved with xylene and total RNA extracted using the High Pure FFPE RNA Micro Kit (Roche, Indianapolis, IN) or Qiagen miRNeasy FFPE kit ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Antiviral azaBINOL B#24 was assessed for inhibition of HIV-1 reverse transcriptase using a commercially available colorimetric reverse transcriptase assay kit (Roche) according to the manufacturer’s instructions ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... subtilis strains at the different time points were evaluated via terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) using the In-Situ Cell Death Detection Kit with fluorescein (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The crosslinked chromatin was lysed in 130 uL of Buffer B (LowCell# ChIP kit, diagenode) supplemented with Complete protease inhibitor (Roche) and 20mM Na-butyrate (Sigma) ...
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bioRxiv - Plant Biology 2019Quote: ... Illumina Paired-End libraries were constructed for 280 accessions using the KAPA Hyper Prep Kit (Kapa Biosystems, Wilmington, Massachusetts, USA) following the manufacturer’s instructions (KR0961 – v5.16) ...
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bioRxiv - Molecular Biology 2020Quote: ... TUNEL staining was performed prior to secondary antibody incubation using the Biotin-based in situ cell death detection kit (Roche) and following the manufacturer instructions ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed with ice-cold DPBS and scraped in ice-cold kit lysis buffer (1 mL/107 cells) containing a cocktail of protease inhibitors (cOmplete, EDTA-free; Roche). Cells were then lysed in a Potter-Elvehjem homogenizer (~50 strokes) ...
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bioRxiv - Bioengineering 2019Quote: ... the entire miniprepped volume was used as template in a PCR reaction using the KAPA HiFi PCR Kit (Kapa Biosystems). A ten-fold dilution of this PCR product was used as template in a barcoding PCR adding Illumina adapter sequences and dual barcodes ...
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bioRxiv - Microbiology 2019Quote: ... And 1.0 μg total RNA was reverse-transcribed into cDNA using the transcriptor first-strand cDNA synthesis kit (Roche, China). qPCR was conducted using FastStart Universal SYBR Green Master (Rox ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The cDNA encoding for the A33 construct was obtained by total RNA extraction from the HT-29 colorectal carcinoma cell line (High Pure RNA Isolation Kit, Roche), reverse transcription (Transcriptor reverse transcriptase ...
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bioRxiv - Genomics 2021Quote: ... were used to prepare single-end (SE) libraries with an insert size of 300 bp using a PCR-free workflow with the KAPA HyperPrep Kit (Roche) following manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... The library was prepared by the Genome Technologies core facility at JAX using the KAPA mRNA HyperPrep Kit (KAPA Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... TUNEL staining was performed on testis sections according to the instructions provided with the cell death detection kit (11684795910, Roche). To reduce experimental variations ...
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bioRxiv - Developmental Biology 2021Quote: ... Template DNAs of interest (500 ng each) were transcribed in vitro during 2 hours at 37°C using SP6 RNA polymerase kit (Roche), followed by a 1 hr treatment with DNAse I (Roche ...
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bioRxiv - Immunology 2021Quote: Strand-specific ribosomal RNA depleted RNA-seq libraries were prepared from 1 µg of total RNA using the KAPA Stranded RNA-seq Kit with Ribo-Erase (Roche). Samples were prepared in at least triplicate for analysis ...
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bioRxiv - Microbiology 2021Quote: ... The 32P-radiolabeled probe was generated from a plasmid containing the entire HPV16 genome by radiolabeling using a Random Prime labeling kit (Roche).
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bioRxiv - Neuroscience 2020Quote: ... Pooled libraries were cleaned using a 0.6:1 ratio of Ampure beads and quantified on Qubit using HS DNA reagents and with the KAPA Library Quantification Kits for Illumina (Roche). Samples were sequenced on two separate rapid-runs on HiSeq2500 (Illumina) ...
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bioRxiv - Developmental Biology 2021Quote: ... were generated cloning each enhancer from the genomic zebrafish DNA DNA using KAPA Long Range HotStart PCR kit (Kapa Biosystems) and cloned into the E1b:GFP:AC-DS vector (102417 Addgene (Chong-Morrison et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Further quantification of the adapter ligated fragments and confirmation of successful P5 and P7 adapter incorporations were assessed with the KAPA universal library quantification kit for Illumina (Roche) run on a ViiA7 real-time PCR system (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: The RNA-Seq libraries were prepared from total RNA using KAPA Stranded mRNA-Seq Kit Illumina Platforms (Roche-Kapa Biosystems) with minor modifications ...
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bioRxiv - Neuroscience 2021Quote: The RNA-Seq libraries were prepared from total RNA using KAPA Stranded mRNA-Seq Kit Illumina Platforms (Roche-Kapa Biosystems) with minor modifications ...
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bioRxiv - Evolutionary Biology 2020Quote: RNA was extracted from 200 μl swab rectal samples or fecal pellets with the High Pure Viral RNA Kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Viral transport media was added to each pool at a 1:1 ratio for nucleic acid extraction performed on the Roche MagNA Pure 24 platform using the MagNA Pure 24 Total NA Isolation kit (Roche). Elution volume was set to 50 ul to concentrate viral RNA ...
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bioRxiv - Systems Biology 2020Quote: ... strand-specific RNA libraries were prepared using a KAPA Stranded RNA-Seq Library Preparation Kit (KAPA Biosystems, catalog no. KK8401) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... was extracted from inactivated samples (200 μL) using the MagNA Pure Compact instrument and MagNA Pure Compact Nucleic Acid Isolation Kit I (Roche Molecular Systems Inc ...
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bioRxiv - Microbiology 2021Quote: ... The tissues were processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159) (Roche Diagnostics Corp. ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then treated with the TUNEL reaction mixture according to the protocol (In situ Cell Death detection Kit, TMR red; Roche) and incubated for 1 h at 37°C in the dark ...
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bioRxiv - Neuroscience 2021Quote: RNA was reverse transcribed using the High Capacity RNA-to-cDNA kit and qPCR performed using a Roche LightCycler480 with SYBR Green I Master Mix (Roche) and the primers listed in Table S2 ...
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bioRxiv - Neuroscience 2020Quote: ... All qPCR reactions were performed in 10 μl volume in triplicates with 1× LightCycler 480 SYBR Green I Master kit (Roche) and primers listed in Supplementary Table 1 on LightCycler® 480 PCR instrument II (Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... Detection and quantification of apoptosis in cell cultures was determined using a In Situ Cell Death Detection Kit (Roche, 11684795910) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Libraries were prepared by the Van Andel Genomics Core from 300 ng of total RNA using the KAPA RNA HyperPrep Kit with RiboseErase (v1.16; Kapa Biosystems). RNA was sheared to 300-400 bp ...
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bioRxiv - Physiology 2021Quote: ... After the concentration of DNA was evaluated by qPCR using the KAPA Library Quantification kit (KAPA Biosystems, Wilmington, MA, USA), the libraries were loaded onto an Illumina MiSeq sequencer and then sequenced using MiSeq Reagent kits v2 500 cycles (Illumina ...
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bioRxiv - Plant Biology 2021Quote: ... the blot was washed at 55°C and probe detection was performed according to the manufacturers’ protocol using the DIG luminescent detection kit (Roche). The intensity of individual bands was measured with respect to the background and integrated density value (IDV ...
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bioRxiv - Bioengineering 2021Quote: ... the probes were detected with anti-digoxigenin and anti-fluorescein antibodies conjugated with alkaline phosphatase (AP) and horseradish peroxidase (HRP) using AP detection kit (Roche) and tyramide signal amplification (TSA ...
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bioRxiv - Microbiology 2020Quote: ... DNA probes for 5S rRNA and SPA0079 were amplified with PCR using primers described in Table S2 and PCR DIG Probe Synthesis Kit (#11636090910, Roche). The reaction was performed with a total volume of 50 μl containing 0.2 μM of forward primer ...