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Citations for Roche :
5301 - 5350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Complete Phosphatase inhibitor cocktail (Roche, 11836145001), 10 mM sodium fluoride ...
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bioRxiv - Molecular Biology 2023Quote: Predigested proteins and peptides were reduced with 10 mM dithiothreitol (DTT) (Roche, 10197777001) for 30 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... (PhosSTOP Roche)] ...
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bioRxiv - Molecular Biology 2023Quote: ... EDTA-free Complete Protease inhibitor (Roche, Mannheim, Germany). Proteins were eluted using a 5mL linear gradient of elution buffer 1 (20mM NaH2PO4 pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... EDTA-free Complete Protease inhibitor (Roche)) at 4° C followed by sonication ...
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bioRxiv - Microbiology 2023Quote: ... we calculated mean Cp values using the second derivative method (2nd derivative Max) implemented in LightCycler 480 SW 1.5 (Roche Applied Science). Replicate was considered as positive when Cp <36 ...
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bioRxiv - Microbiology 2023Quote: ... and S-D-Bact-0785-a-A-21 (GACTACHVGGGTATCTAATCC) (Klindworth et al. 2013) together with KAPA HIFI Hot Start Ready Mix (Kapa Biosystems, Cat. No. 07958935001) containing the polymerase with proofreading activity ...
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bioRxiv - Microbiology 2023Quote: ... no-chloroplast amplifying primers 335F (CADACTCCTACGGGAGGC) and 769R (ATCCTGTTTGMTMCCCVCRC) (Dorn-In et al. 2015) were used together with KAPA2G Robust PCR Kits (Kapa Biosystems, Cat. No. KK5005, Wilmington, USA). In protocol 2 (P2) ...
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bioRxiv - Microbiology 2023Quote: ... in combination with the KAPA stranded mRNA seq kit (Kapa Biosystems, Wilmington, MA, USA). Ribo-depleted RNA was suspended in the Fragment/Prime/Elute mix and fragmented at 94°C for eight min ...
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bioRxiv - Microbiology 2023Quote: ... The nucleus was stained with 4,6-Diamidino-2-phenylindole (DAPI, 1 µg/mL in PBS) (Roche; Basel, Switzerland). Finally ...
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bioRxiv - Microbiology 2023Quote: ... Pre-capture libraries were prepared from up to 100 ng input DNA using the KAPA Hyperplus kit (Roche) and TruSeq adapters and barcoded primers (Illumina) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 1000 µL cold cytoplasmic lysis buffer (10 mM HEPES pH 7.9, 10 mM KCl, 2 mM Mg acetate, 3 mM CaCl2, 340 mM sucrose, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA to 1× ...
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bioRxiv - Microbiology 2023Quote: ... cells were resuspended in 1000 µL cold sucrose buffer containing (10 mM HEPES pH 7.9, 10 mM KCl, 2 mM Mg acetate, 3 mM CaCl2, 340 mM sucrose, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA to 1× ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were then resuspended in 1000 µL cold nucleoplasm extraction buffer (50 mM HEPES pH 7.9, 150 mM K Acetate, 1.5 mM MgCl2, 0.1% [vol/vol] Nonidet P40, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA free ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were collected as the cytoplasmic fraction and pellets were resuspended in 200 µL cold nuclear lysis buffer (20 mM HEPES pH 7.9, 100 mM NaCl, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA free ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in the same buffer supplemented with a protease inhibitor cocktail (Roche). Cells were disrupted by sonication on ice (40% power ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots (6 μL) of tg7-brain homogenates were supplemented with a mixture of protease inhibitors (Roche), mixed with 5% glucose (94 μL ...
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bioRxiv - Microbiology 2023Quote: ... then DNA was extracted on the MagNA Pure 24 (Roche Molecular Systems). The DNA was prepared for sequencing with the SQK-LSK109 Ligation Sequencing Kit with NBD104/114 barcoding ...
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bioRxiv - Microbiology 2023Quote: ... with a KABA SYBR Fast Universal qPCR Kit (Kapa Biosystems, Wilmington, MA, USA) was used for quantification ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then washed 4 times in wash buffer containing 25% formamide (Roche, 11814320001), 2x SSC (Ambion ...
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bioRxiv - Microbiology 2023Quote: ... 1x protease inhibitor cocktail (Roche));
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bioRxiv - Microbiology 2023Quote: ... 1x protease inhibitor cocktail (Roche));
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bioRxiv - Microbiology 2023Quote: ... The coding sequence of SifA amplified from SL1344 genome were cloned in a series of prokaryotic expression vectors including pBH-HA from Roche, pET28a ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with complete mini EDTA-free protease inhibitor (Roche, Basel, Switzerland) and phenylmethene-sulfonylfluoride PMSF (10 mM ...
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bioRxiv - Molecular Biology 2023Quote: ... Then 2 µL of 20 mg/mL Proteinase K (#3115879001, Roche) was added and again incubated at 55°C for 2 h ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR primers for Rpl8 and Ifna4 were used as given above together with the universal probe library probes (UPL, Roche) #5 and #3 ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were developed with Lumi-Light (#12015200001, Roche) or Pierce™ ECL (#32106 ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative RT-PCR was performed on a LightCycler96 system (Roche) with FastStart Essential DNA Green Master kit (Roche ...
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bioRxiv - Microbiology 2023Quote: ... All steps were performed at 4°C and buffers were all supplemented with protease inhibitor cocktail from Roche (cOmplete tablets, EASYpack). The pellets were washed once in buffer A (50 mM Tris/HCl pH8.0 ...
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bioRxiv - Microbiology 2023Quote: ... 167 mM NaCl) containing protease inhibitors (Roche) and pre-cleared with 80 μl of Protein-A agarose (Roche ...
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bioRxiv - Microbiology 2023Quote: ... After the membrane was blocked with 5% BSA (Bovine Serum Albumin Fraction V, Roche Diagnostics, Germany) in TBST buffer (1X Tris-Buffered saline ...
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bioRxiv - Microbiology 2023Quote: ... containing 1x complete protease inhibitor cocktail (Roche) at 4°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... complete proteinase inhibitor cocktail (Roche)) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were incubated for 20 min with a mix of XtremeGENE9 transfection reagent (Roche) and OptiMEM (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5µL Proteinase K (Roche), 0.5µL 1% SDS) ...
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bioRxiv - Microbiology 2023Quote: ... Whole genome amplification of HPV16 from oral cancer DNA was performed with an Expand High Fidelity PCR system (Roche) according to the manufacturer’s instructions using HPV type 16-specific primers ...
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bioRxiv - Molecular Biology 2023Quote: ... with 1 mM DTT and Protease Inhibitor Cocktail (PIC, Roche) added immediately prior to use] at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Protease Inhibitor Cocktail (PIC) (Roche), fractionated by SDS-PAGE and transferred to nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2023Quote: ... protease inhibitor cocktail (Roche). After protein quantification ...
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bioRxiv - Molecular Biology 2023Quote: ... Ct values were calculated by LightCycler 480 Software (Roche) and normalized using 16S rRNA as a housekeeping gene ...
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bioRxiv - Molecular Biology 2023Quote: ... Gene expression was analyzed by quantitative PCR (qPCR) in 384-well plates using the LightCycler480 (Roche). For each tissue ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 tauRD biosensor cell pellets were lysed by resuspension in cold 0.01% Triton in PBS containing cOmplete™ mini EDTA-free protease inhibitor tablet (Roche) at a concentration of 10% w/vol followed by incubation in the lysis buffer for 15 minutes on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... and DS10 cell pellets were lysed by resuspension in cold 0.05% Triton in PBS containing cOmplete™ mini EDTA-free protease inhibitor tablet (Roche) at a concentration of 10% w/vol followed by incubation in the lysis buffer for 20 minutes on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... and each was lysed by trituration in 1 mL ice-cold PBS with 0.25% Triton-X containing cOmplete™ mini EDTA-free protease inhibitor cocktail tablet (Roche) at a concentration of 10% w/vol followed by a 15-minute incubation on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... Protease inhibitors (PI, cOmplete, #4693116001) and phosphatase inhibitors (PhI, PhosSTOP, #4906837001) were from Roche (Mannheim, Germany). Recombinant murine IFNβ (#12405-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... on a LightCycler 96 instrument (Roche). qPCR primers were as follows ...
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bioRxiv - Physiology 2023Quote: RIPA buffer supplemented with cOmplete Protease Inhibitor (Roche) and PhosSTOP (Roche ...
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bioRxiv - Physiology 2023Quote: ... for 45 seconds at 6,000 rpm × 3 (and placed on ice for 5 min at the end of each 45 second homogenization) using a Roche Magnalyser instrument (Roche, Germany). RNA was then isolated as per Invitrogen’s manufacturer’s instructions for Tri-reagent ...
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bioRxiv - Physiology 2023Quote: ... 8µl were used to determine blood triglyceride concentration (mg/dl) using Accutrend-plus (Roche). The excess blood was collected in a 1.5ml tube ...
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bioRxiv - Physiology 2023Quote: ... Two ul of blood were immediately used to determine blood glucose concentration (mg/dl) using the glucometer Accu-check guide (Roche). In contrast ...