Labshake search
Citations for Roche :
5251 - 5300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and KAPA qPCR (Roche). Sequencing was performed at TCAG on the Illumina HiSeq 2500 with single-end 100bp read length yielding 40 to 50 million reads.
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bioRxiv - Microbiology 2023Quote: ... Reactions were run on a LightCycler® 96 instrument (Roche) and data was analyzed using the 2-ΔΔCt method ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR reactions were set up using the FastStart Essential DNA Green Master kit (Roche), using four different primer pairs ...
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bioRxiv - Plant Biology 2023Quote: ... using a LightCycler 480 II instrument (Roche Applied Science), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Sst sense and antisense probes were transcribed using a DIG or FITC RNA labeling kit (Roche) and purified with RNA Clean & Concentrator (Zymo Research) ...
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bioRxiv - Neuroscience 2023Quote: ... the nonspecific staining was blocked with 10% normal donkey serum in PBS with 1% BSA and 0.3% triton for 1 hour and then incubated with mouse anti-BrdU (1: 1000, Roche), rabbit anti-Ki67 (1 ...
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bioRxiv - Neuroscience 2023Quote: ... with 3 mg/mL DNAse I grade II (Roche, Cat# 104159). The cell suspension was centrifuged at 1,300 rpm for 5 minutes with 7.5% BSA solution (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... isolated DRGs were placed in 1.5 mL screw cap polypropylene tubes in the presence of lysis buffer consisting of 50 mM Tris-HCl pH 7.5 containing a protease inhibitor cocktail (Roche). Glass beads (0.5–1.0 mm ...
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bioRxiv - Neuroscience 2023Quote: RNA-Seq library was prepared using the KAPA Stranded RNA-Seq Kit with RiboErase (Kapa Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and cOmpleteTM Protease Inhibitor Cocktail (5056489001, Roche, Ireland, 1:25) through mechanical disruption using Qiagen TissueLyser LT (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... phosphatase inhibitor (Roche), and 5 µM trichostatin A (TSA ...
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bioRxiv - Neuroscience 2023Quote: ... plus protease inhibitor cocktail (Roche), phosphatase inhibitor (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... 50 mM dithiothreitol (DTT) plus protease inhibitors (Roche). After incubation (30 min in a shaker at 25ºC) ...
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bioRxiv - Neuroscience 2023Quote: TUNEL assay was carried out following the manufacturer’s instructions (In Situ Cell Death Detection Kit, Roche-11684795910).
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bioRxiv - Neuroscience 2023Quote: ... supplemented with protease and phosphatase inhibitor cocktail (Roche), sonicated for 5 min and centrifuged at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with EDTA-free protease inhibitors (cOmplete, Roche) and proteins were extracted as explained above ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time PCR was carried out on a LightCycer480-II System (Roche Diagnostics, Penzberg Germany) by using SYBR Premix Ex Taq TM (TAKARA ...
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bioRxiv - Neuroscience 2023Quote: ... and 1xcOmplete™ protease inhibitor cocktail (Roche). Cells were lysed with microfluidizer then boiled for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... The plate was inserted in the Roche LightCycler® 480 (Roche Life Science, Switzerland) and the cycles were defined as follows ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were then dried and cut into small pieces before the pronase treatment (15 mg pronase per hemisphere, (Roche, cat. no. 11459643001) in 0.1 M Trizma hydrochloride (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was diluted to 2 ng/µL and 4 µL were added to 10 µL 2x FastStart Universal SYBR Green PCR Master (Roche). Each sample was run in triplicate using iTaq Universal SYBR Green Supermix (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected according to the manufacturer’s protocol at a µL lipofectamine: µg plasmid ratio of 3:1 (X-tremeGENE 9, Roche). After 48 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative RT-PCR was performed with a Light Cycler 480 SYBR Green IMaster (Roche Applied Science) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was digested with DNase-free RNase (5 μg/ml, Roche #11119915001) for 1 hour at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Library concentrations were measured using KAPA Illumina Library Quantification Kit (KAPA Biosystems, Wilmington, MA). Polled libraries were sequenced on Illumina NovaSeq6000 sequencer with 150-bp Paired-End chemistry (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... further containing the suggested amount of protease inhibitors mixture according to the manufacturer’s instructions (Roche). The sample was centrifuged at 16,000 g for 10 min at 4°C and guanidinium hydrochloride was added to the cleared supernatant to reach a final concentration of 4 M.
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bioRxiv - Plant Biology 2023Quote: The YFP digoxygenin-labelled antisense and sense RNA probes were generated by in vitro transcription according to the instructions provided with the DIG RNA labelling kit (SP6/T7; Roche) using a plasmid containing the YFP sequence as template ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μl of 1:25 diluted cDNA with water was used for real-time PCR (LightCycler 480 Roche) using SYBR Premix Ex Taq (Takara ...
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bioRxiv - Plant Biology 2023Quote: ... in 100μl GTEN-buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl,1 x protease inhibitors (Roche), 5mM DTT ...
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bioRxiv - Plant Biology 2023Quote: ... together with the primers from the Kapa Hyper Prep kit (Roche) and MyTaq Master mix (Bioline) ...
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bioRxiv - Plant Biology 2023Quote: ... and then used to construct libraries with Kapa HyperPrep Kit (Roche). The libraries were sequenced on an Illumina iSeq 100 sequencer.
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bioRxiv - Plant Biology 2023Quote: ... and 1x Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Pathology 2023Quote: ... IHC staining for GPER was performed using the Ventana Benchmark Ultra platform (Roche Diagnostics). Tissue sections were cut onto positively charged slides at a thickness of 3-5 microns and baked at 60-65 degrees Celsius for 10 minutes prior to IHC staining ...
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bioRxiv - Physiology 2023Quote: ... DNA was isolated with the MagNaPure System (Roche Diagnostics, Mannheim, Germany) from mononuclear cells after lysis of erythrocytes ...
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bioRxiv - Plant Biology 2023Quote: ... A GTEN-based buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl, 1 x protease inhibitors (Roche), 5mM DTT ...
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bioRxiv - Plant Biology 2023Quote: The broad-spectrum phosphatase inhibitor (PhosSTOP, Phosphatase Inhibitor Cocktail Tablet, Roche) was dissolved in MQ water and 1x solution was used for the treatments ...
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bioRxiv - Plant Biology 2023Quote: ... and 1x Protease Inhibitor Cocktail (Roche)) on ice for 10 minutes and the lysate was sheared by Bioruptor Plus (Diagenode ...
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bioRxiv - Plant Biology 2023Quote: ... 5 mM Benzamidine and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche)) was added and mixed by pipetting ...
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bioRxiv - Plant Biology 2023Quote: ... and 1x Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Plant Biology 2023Quote: ... 5mM Benzamidine and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Plant Biology 2023Quote: ... and 1X Protease Inhibitor Cocktail (Roche)) for 10 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... together with the primers from the Kapa Hyper Prep kit (Roche) and MyTaq Master mix (Bioline ...
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bioRxiv - Plant Biology 2023Quote: ... and 1x Protease inhibitor (Roche)) ...
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bioRxiv - Plant Biology 2023Quote: ... Libraries were prepared with the purified PCR product by the Kapa DNA Hyper Kit (Roche) together with TruSeq DNA UD indexes for Illumina (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... The DNA fragments were used to perform end repair reaction using the Kapa Hyper Prep kit (Roche), and together with TruSeq DNA Sgl Index Set A/B (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... cOmplete EDTA-free Protease Inhibitor Cocktail (Roche), 50uM MG132) ...
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bioRxiv - Plant Biology 2023Quote: ... 50 uM MG132 and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche)) and further homogenized with dounce homogenizer ...
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bioRxiv - Plant Biology 2023Quote: ... The DNA fragments were used to perform end repair reaction using the Kapa Hyper Prep kit (Roche), and together with Illumina TruSeq DNA sgl Index Set A/B (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were: mouse anti-GFP (Roche, 11814460001, 0.4 µg/mL), rabbit anti-Cdc2 (Santa Cruz ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was extracted using acidic phenol chloroform and treated with DNase (Roche DNase I) to remove any remaining DNA contamination ...