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Citations for Roche :
5151 - 5200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... protease inhibitors (Roche complete, EDTA free) and 0.025% of Igepal CA-630 ...
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bioRxiv - Molecular Biology 2023Quote: ... Worm powder was resuspended in cold PBS (supplemented with Roche cOmplete protease inhibitors). Freshly prepared EGS solution (ethylene glycol bis(succinimidyl succinate) ...
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bioRxiv - Molecular Biology 2023Quote: ... or pre-extracted for 5 minutes on ice with CSK/0.5% Triton buffer (supplemented with Roche Complete Protease inhibitor and PhosSTOP Phosphatase inhibitors ...
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bioRxiv - Molecular Biology 2023Quote: ... total RNA (50 μg) was separated on 1% agarose–formaldehyde gels and transferred to Hybond-N+ nylon membranes (Roche, Basel, Switzerland). Northern blotting was conducted with biotin-labeled DNA (bio-CTGTAGAAAGTCTGCTGATCGATACCGCGACG ...
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bioRxiv - Molecular Biology 2023Quote: ... and pIZT-CS2 (1 μg) containing the complete cDNA sequence of BmCPV S2 dsRNA encoding RdRp using Roche-X Gem (Switzerland, Roche, Cat: 6366236001). At 48 h posttransfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantification of the RT-PCR product was obtained on a LightCycler 480 (Roche).
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bioRxiv - Molecular Biology 2023Quote: ... Urine was analyzed for Protein and Albumin (Cobas c 311 Analyzer; Roche Diagnostics).
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bioRxiv - Molecular Biology 2023Quote: ... using the LightCycler Fast Start DNA Master SYBR Green I kit (Roche Diagnostics) as previously described (Maire et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of total RNA from each sample was converted into cDNA with First Strand cDNA Synthesis Kit (Roche, #04897030001). Primers used in the RT-qPCR are listed in Supplementary Table S1 ...
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bioRxiv - Molecular Biology 2023Quote: ... one tablet of protease inhibitor cocktail (Roche) and 1 mg DNase I (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1% deoxycholate) supplemented with protease inhibitor cocktail (Roche). Cell lysates were incubated on ice for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... EDTA-free protease inhibitor (Roche) and cycloheximide or puromycin.
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% SDS) supplemented with protease and phosphatase inhibitors (both Roche) for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: Real-time RT-PCR were performed with the Roche Lightcycler 480 system (Roche Diagnostics, Neuilly-Sur-Seine, France). The mix of the reaction was 6 μL per sample ...
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bioRxiv - Molecular Biology 2023Quote: ... 1mM PMSF (PanReac AppliChem, Barcelona, Spain) and EDTA-free protease inhibitor complex (cOmplete Tablets EDTA-free, Roche, Mannheim, BW, Germany). Samples were then sonicated in a Bioruptor® sonication device (Diagenode ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: rat anti HA (1:500, Roche, #11-867-423-001), chicken anti GFP (1:800 ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA was extracted using TriPure Isolation Reagent (Roche, #1166715700). Quality and quantity of the isolated RNA were determined with NanoDrop One (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were ruptured using a beadbeater with 1.9g of glass beads either in SDS buffer (1X phosphate buffered saline, 10% Glycerol, 2mM EDTA, 2mM DTT, Roche protease inhibitor cocktail ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time reverse transcription PCR assays were conducted in 96-well plates using the LightCycler 480 Instrument (Roche, Wilmington, MA). Levels of mRNA expression were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) ...
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bioRxiv - Molecular Biology 2023Quote: ... using a Cobas creatinine reagent (Roche Diagnostics, Indianapolis, Indiana). The plasma creatinine values were used to calculate estimated glomerular filtration rate (eGFR ...
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bioRxiv - Molecular Biology 2023Quote: ... or monoclonal rat anti-HA antibody (Roche; Basel, CH; dilution 1:200) and polyclonal rabbit anti-Pf39 antisera (dilution 1:10,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... monoclonal mouse anti-GFP antibody (Roche; Basel, CH) and monoclonal rat anti-HA antibody (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... The membranes were developed in a NBT/BCIP solution (nitroblue tetrazolium chloride/5-bromo-4-chloro-3-indoxyl phosphate; Roche; Basel, CH) for up to 20 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... and monoclonal rat anti-HA antibody (Roche; Basel, CH).
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bioRxiv - Molecular Biology 2023Quote: ... pH 8.0) supplemented with protease inhibitor cocktail (PIC, Roche; Basel, CH). A 5x SDS loading buffer with 25 mM dithiothreitol was added to the lysates ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were incubated overnight at 4°C with monoclonal mouse anti-GFP antibody (Roche; Basel, CH; dilution 1:500) or monoclonal rat anti-HA antibody (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... protease cOmplete Mini tablet (Roche)) was added to each well ...
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Membrane-dependent actin polymerization mediated by the Legionella pneumophila effector protein MavHbioRxiv - Molecular Biology 2023Quote: ... and protease inhibitor cocktail (Roche)) ...
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bioRxiv - Molecular Biology 2023Quote: ... Library construction of 300 ng total RNA for each sample was made using KAPA Stranded mRNA-Seq Kit (Illumina) (Kapa Biosystems), using 10 cycles of PCR amplification ...
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bioRxiv - Molecular Biology 2023Quote: ... and phosphatase inhibitors (PhosSTOP, Roche). Lysates were sonicated and debris was pelleted by centrifugation at 10,000 × g for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 150mM NaCl) supplemented with protease (Roche Complete) and phosphatase (PhosStop ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified with the High-Pure PCR Product Purification kit (Roche) and the library was sequenced by Illumina X ten platform.
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified with the High-Pure PCR Product Purification kit (Roche) and then sequenced by the X ten platform.
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bioRxiv - Molecular Biology 2023Quote: ... product was generated with KAPA2G Robust polymerase (KAPA biosystems), manufacturer provided buffer per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 mg DNase I (Roche). After incubation on ice for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... containing protease inhibitor (Roche) and phosphatase inhibitor (Merck) ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with cOmplete protease inhibitor cocktail (Roche) and Halt phosphatase inhibitor cocktail (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with c0mplete tablet mini-EDTA-free protease inhibitor (Roche) and Halt phosphatase inhibitor cocktail (Thermo Fisher Scientific)) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then lysed in RIPA buffer with 1× EDTA-free protease inhibitor cocktail (Roche) and analyzed using western blots.
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bioRxiv - Molecular Biology 2023Quote: ... upon which cells were washed with 2x ice-cold PBS and lysed in RIPA buffer with 1× EDTA-free protease inhibitor cocktail (Roche). Lysates were then separated on a 17.5% acrylamide gel for 6h at 150V and transferred onto a 0.22µM nitrocellulose membrane using semi-dry transfer ...
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bioRxiv - Molecular Biology 2023Quote: ... 5×106 K562 cells were resuspended in PBS with EDTA-free protease inhibitor cocktail (Roche) and lysed using a 27.5G needle ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then resuspended in 1mL PBS with 1× EDTA-free protease inhibitor cocktail (Roche) from which 5 μl of the cells were lysed in 50 μl of 1% Triton lysis buffer to obtain whole cell protein levels ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1× EDTA-free protease inhibitor cocktail (Roche)) ...
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bioRxiv - Molecular Biology 2023Quote: ... with the high-throughput Light Cycler 480 II Real-Time PCR system(Roche, Germany, Mannheim). cDNA samples were diluted with Nuclease Free Water (1:5) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was isolated using High Pure miRNA Isolation Kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions and stored at −80°C until use ...
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bioRxiv - Molecular Biology 2023Quote: ... 1mM DTT) supplemented with protease inhibitors (1X Complete Protease Inhibitor Cocktail EDTA-free, Roche). After centrifugation at 20,000 g for 20 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The qPCR was performed using LightCycler 480 Instrument II (Roche) and the relative gene expression was calculated using the 2-(ΔΔCt ...
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bioRxiv - Molecular Biology 2023Quote: ... and DNA concentration was quantified using the KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems) on a BioRad CFX Machine ...
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bioRxiv - Molecular Biology 2023Quote: ... Then the mRNA expression was analyzed quantitatively using the SYBR Green Supermix (Roche, Basel, Switzerland), and the relative gene expression data were analyzed using the relative 2−ΔΔct method (45).
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bioRxiv - Molecular Biology 2023Quote: ... the Roche LightCycler® 480 System (Cat# 05015243001) in combination with 384-well plates (FrameStar, Cat# 4ti-0381/DBC, and Roche, Cat# 04729757001) was used ...