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Citations for Merck :
401 - 450 of 779 citations for Recombinant Human V erb b2 Erythroblastic Leukemia Viral Oncogene Homolog avian His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 0.4 mg/ml collagenase from Clostridium histolyticum type V (Merck), and ROCK inhibitor Y-27632 ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: A549 (human epithelial lung cell line) and EA.hy926 (human endothelial somatic cell line) cells were bought from Merck company and ATCC ...
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bioRxiv - Biochemistry 2023Quote: ... and transiently transfected with the plasmid DNA containing GFP-tagged protein of interest (See Table S7 for plasmid constructs) using GeneJuice transfection reagent (70967, Merck) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Lysates from HEK293 cells overexpressing FLAG-tagged wild typeTP53INP2 or the TP53INP2-3A were affinity-purified with anti-FLAG-M2 affinity beads (Merck). Then ...
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bioRxiv - Molecular Biology 2022Quote: CobB driven deacetylation of His-PrsAc was analyzed by Western blot with anti-acetyl lysine antibodies (Sigma/Merck) and mass spectrometry ...
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Oxidative and non-oxidative active turnover of genomic methylcytosine in distinct pluripotent statesbioRxiv - Cell Biology 2020Quote: ... 10 μg/ml human holo-Transferrin (Merck), 12.5 mg/ml AlbuMAX I (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... and human plasma full-length fibronectin (Merck) was added to the dish at a final concentration of 10 µg/µl for a 1h incubation at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... and human plasma full-length fibronectin (Merck) was added to the dish at a final concentration of 10 μg/μl for a 1h incubation at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... mouse anti-human α5 IgG1 (JBS5; Merck), mouse anti-human α5 IgG3 (P1D6 ...
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bioRxiv - Physiology 2022Quote: ... labelled human mitochondria primary antibody (Merck, MAB1273), 1:500 for 48 h at 4°C and washed with PBS overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... rat anti-human β1 IgG1 (AIIB2; Merck), mouse anti-human β1 IgG1 (Lia1/2 ...
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bioRxiv - Microbiology 2023Quote: ... mouse anti-human α5 IgG3 (P1D6; Merck), mouse IgG1 (JC1 ...
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: ... 180⍰µg/ml Human transferrin (Merck, T8158), 20% D4T Conditioned Media ...
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bioRxiv - Immunology 2023Quote: ... polyclonal anti-human PI3K p85 (Merck Millipore), anti-PI3 Kinase p110α (C73F8 ...
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bioRxiv - Cell Biology 2024Quote: ... or 5µg/cm2 human-plasma fibronectin (Merck) overnight in 4°C ...
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bioRxiv - Immunology 2021Quote: ... or 1 × 105 BM-DCs (mouse) per condition were pre-incubated for 1h prior to viral stimulation or infection with inhibitors MG132 (10μM; Merck Millipore) BafA1 (0.5μM ...
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bioRxiv - Neuroscience 2020Quote: ... culture medium was collected and viral particles were enriched by filter device (Amicon Ultra-15 Centrifuge Filters, Merck, Cat#UFC910008). Titer check was performed on HEK293T cell culture for every batch of lentiviral preparation.
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bioRxiv - Neuroscience 2020Quote: ... culture medium was collected and viral particles were enriched by filter device (Amicon Ultra-15 Centrifuge Filters, Merck, Cat#UFC910008). Titer check was performed on HEK293T cell culture for every batch of lentiviral preparation.
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bioRxiv - Cell Biology 2022Quote: ... The viral supernatant was collected after 48 h and 72 h and filtered through a 0.45 µm PVDF membrane (Merck Millipore).
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bioRxiv - Neuroscience 2023Quote: ... culture medium was collected and viral particles were enriched by filter device (Amicon Ultra-15 Centrifuge Filters, Merck, Cat#UFC910008). Titer check was performed on HEK-293T cell culture for every batch of lentiviral preparation.
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bioRxiv - Microbiology 2023Quote: ... The volume of concentrated viral fractions (>50 kDa) was further reduced using Amicon Ultra 15 ml Centrifugal Filters (Merck-Millipore) prior to phenol/chloroform extraction ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Target cells were transduced with 0.5 mL of viral supernatant in 3 mL of total medium supplemented with 8 µg/mL polybrene (Merck Sigma). 2 to 4 days post transduction ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA tagged with 6xHis at the C-terminus was introduced into a pNG2 vector (a derivative of pET-3a, Merck-Novagen) linearized with NdeI and BamHI ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing the desired His6-tagged protein were concentrated to 2 ml using Amicon® Ultra Centrifugal Filters (10,000 MWCO, Merck Millipore), and were directly injected into a size-exclusion chromatography column (Superdex 75 16/60 ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 hrs the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore). The HeLa cells stably expressing mouse Cx26 were originally obtained from Dr K ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore).
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bioRxiv - Neuroscience 2021Quote: ... pH 9.0 buffer containing protease inhibitors (10% w/v, cOmpleteTM, Merck). Subsequently ...
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bioRxiv - Biophysics 2020Quote: ... 50 µl of 5% w/v (145000 g/mol) PVA (Merck) solution in water was spread on a cleaned (rinsed in ethanol and double distilled water ...
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bioRxiv - Biophysics 2022Quote: ... N-hydroxy-succinimide ester (0.1%w/v, Merck Chemicals GmbH, Germany) was added to the oil solution to functionalize the phantoms with Alexa 488 ...
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bioRxiv - Biochemistry 2022Quote: ... 1% (w/v) fatty acid-free bovine serum albumin (BSA, Merck) and 1x EDTA-free cOmplete™ protease inhibitor cocktail (Roche ...
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bioRxiv - Immunology 2022Quote: ... and subsequently perfused with 4% w/v paraformaldehyde (PFA) (1.04005.100, Merck) in PBS to fix the tissues ...
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LptM promotes oxidative maturation of the lipopolysaccharide translocon by substrate binding mimicrybioRxiv - Microbiology 2023Quote: ... and 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Merck) or 1% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... on polystyrene culture dishes coated with 0.1% (w/v) Gelatin (Merck) solution ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 100μL of 10% w/v Chelex 100 (Merck Life Sciences, UK), 2ul of 20ng/μL Proteinase K (Merck Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... we used commercially available BSA Cohn fraction V (Merck, Darmstadt, Germany), unless otherwise stated ...
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bioRxiv - Neuroscience 2023Quote: ... and 1x Phosphatase Inhibitor Cocktail Set V (Merck, cat. no 524629). First ...
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bioRxiv - Bioengineering 2023Quote: ... and mounted on 1.2 % w/v/ low-melting point agarose (Merck). The embryos were wounded by making a single incision using a pair of forceps (Dumont No ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (10,000 x g, 30 min, RT) and incubated for 1 hour with Ni-NTA His bind resin (Merck) rolling at RT ...
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bioRxiv - Cancer Biology 2019Quote: The uncharged polystyrene 96-well plate were coated with different recombinant proteins-Collagen IV (Merck Millipore), P-Selectin ...
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bioRxiv - Biophysics 2022Quote: ... Both recombinant and synthetic lyophilized peptides were dissolved in 6M guanidine thiocyanate (Merck KGaA, Darmstadt, Germany) and incubated overnight at 1250 rpm ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck, Darmstadt, Germany), upon induction with 0.6□mM IPTG at 0.8 OD600 ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with F(ab’)2-Goat anti-Human IgG-FITC (Merck Goat anti-Human IgG, F(ab’)2 ...
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bioRxiv - Cancer Biology 2023Quote: ... the viral supernatant was concentrated ∼2000 fold using a 100kDa MW cut-off Millipore Centricon ® 70 Plus (Merck Millipore; UFC710008). The virus was further concentrated by ultracentrifugation using the SW 55 Ti rotor for the Beckman Coulter Optima TM L-90 Ultracentrifuge at 45000 RPM / 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Cells were permeabilized with 100% ice-cold methanol for 20 min at -20°C and rehydrated by washing with PBS and then stained for viral hexon protein expression with an anti-hexon primary antibody (mouse; Merck; #MAB8052) and an anti-mouse secondary antibody coupled to AF488 or AF647 (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: Human thrombin was purchased from Merck (Cramlington, UK) and reconstituted in double distilled water (ddH2O) ...
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bioRxiv - Microbiology 2021Quote: ... or polyclonal anti-human antibody (I2136, Merck, Germany) was coupled simultaneously in four flow cells ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-human nuclei (Merck, MAB4383, 1:250) were diluted in the blocking solution and incubated overnight on a shaker at 4◦C ...
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Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblybioRxiv - Cell Biology 2022Quote: ... human Ndc80 siRNA (HA12977117-004; Merck, Darmstadt, Germany), human ON-TARGET PRC1 siRNA (L-019491-00-0020 ...