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Citations for Merck :
451 - 500 of 828 citations for Recombinant Human V erb b2 Erythroblastic Leukemia Viral Oncogene Homolog avian His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (10,000 x g, 30 min, RT) and incubated for 1 hour with Ni-NTA His bind resin (Merck) rolling at RT ...
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bioRxiv - Neuroscience 2021Quote: ... pH 9.0 buffer containing protease inhibitors (10% w/v, cOmpleteTM, Merck). Subsequently ...
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bioRxiv - Biophysics 2020Quote: ... 50 µl of 5% w/v (145000 g/mol) PVA (Merck) solution in water was spread on a cleaned (rinsed in ethanol and double distilled water ...
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bioRxiv - Biophysics 2022Quote: ... N-hydroxy-succinimide ester (0.1%w/v, Merck Chemicals GmbH, Germany) was added to the oil solution to functionalize the phantoms with Alexa 488 ...
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bioRxiv - Biochemistry 2022Quote: ... 1% (w/v) fatty acid-free bovine serum albumin (BSA, Merck) and 1x EDTA-free cOmplete™ protease inhibitor cocktail (Roche ...
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bioRxiv - Immunology 2022Quote: ... and subsequently perfused with 4% w/v paraformaldehyde (PFA) (1.04005.100, Merck) in PBS to fix the tissues ...
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LptM promotes oxidative maturation of the lipopolysaccharide translocon by substrate binding mimicrybioRxiv - Microbiology 2023Quote: ... and 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Merck) or 1% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... on polystyrene culture dishes coated with 0.1% (w/v) Gelatin (Merck) solution ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 100μL of 10% w/v Chelex 100 (Merck Life Sciences, UK), 2ul of 20ng/μL Proteinase K (Merck Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... we used commercially available BSA Cohn fraction V (Merck, Darmstadt, Germany), unless otherwise stated ...
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bioRxiv - Neuroscience 2023Quote: ... and 1x Phosphatase Inhibitor Cocktail Set V (Merck, cat. no 524629). First ...
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bioRxiv - Bioengineering 2023Quote: ... and mounted on 1.2 % w/v/ low-melting point agarose (Merck). The embryos were wounded by making a single incision using a pair of forceps (Dumont No ...
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bioRxiv - Neuroscience 2024Quote: ... fixation was performed with 4% w/v Paraformaldehyde (PFA) (Merck/Sigma), pH 7.4 ...
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bioRxiv - Cancer Biology 2019Quote: The uncharged polystyrene 96-well plate were coated with different recombinant proteins-Collagen IV (Merck Millipore), P-Selectin ...
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bioRxiv - Biophysics 2022Quote: ... Both recombinant and synthetic lyophilized peptides were dissolved in 6M guanidine thiocyanate (Merck KGaA, Darmstadt, Germany) and incubated overnight at 1250 rpm ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck, Darmstadt, Germany), upon induction with 0.6□mM IPTG at 0.8 OD600 ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with F(ab’)2-Goat anti-Human IgG-FITC (Merck Goat anti-Human IgG, F(ab’)2 ...
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bioRxiv - Cancer Biology 2023Quote: ... the viral supernatant was concentrated ∼2000 fold using a 100kDa MW cut-off Millipore Centricon ® 70 Plus (Merck Millipore; UFC710008). The virus was further concentrated by ultracentrifugation using the SW 55 Ti rotor for the Beckman Coulter Optima TM L-90 Ultracentrifuge at 45000 RPM / 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Cells were permeabilized with 100% ice-cold methanol for 20 min at -20°C and rehydrated by washing with PBS and then stained for viral hexon protein expression with an anti-hexon primary antibody (mouse; Merck; #MAB8052) and an anti-mouse secondary antibody coupled to AF488 or AF647 (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: Human thrombin was purchased from Merck (Cramlington, UK) and reconstituted in double distilled water (ddH2O) ...
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bioRxiv - Microbiology 2021Quote: ... or polyclonal anti-human antibody (I2136, Merck, Germany) was coupled simultaneously in four flow cells ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-human nuclei (Merck, MAB4383, 1:250) were diluted in the blocking solution and incubated overnight on a shaker at 4◦C ...
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Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblybioRxiv - Cell Biology 2022Quote: ... human Ndc80 siRNA (HA12977117-004; Merck, Darmstadt, Germany), human ON-TARGET PRC1 siRNA (L-019491-00-0020 ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.1% aprotinin and 0.1% Human Insulin Solution (Merck). Medium was changed every other day with EHT medium.
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bioRxiv - Cancer Biology 2019Quote: ... mouse anti-human nuclear antigen (1:100; Merck), Alexa Fluor 647-conjugated rat anti-CD45 (1:100 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HTF medium (EmbryoMax Human Tubal Fluid; Merck Millipore) was mixed 1:1 with mineral oil (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Normal human epidermal keratinocytes were obtained from Merck. All lines were maintained in 3:1 DMEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... growth in LB and human serum (Merck #H4522) was measured ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells transfected with Flag-tagged NSP1 or vector plasmid were treated with the following compounds for 6 h: 10 μM of MG132 (Merck, Cat# 474787), 50 μM of chloroquine (CQ ...
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bioRxiv - Biochemistry 2023Quote: Total cell lysates of HEK293 cells stably expressing C-terminally Flag M2-tagged SIRT4 were incubated overnight at 4°C with anti-Flag M2 agarose beads (Merck, Darmstadt, Germany). The beads were washed three times with washing buffer (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... and applied directly onto a column of 2 ml pre-equilibrated Ni-NTA beads (cOmplete His-Tag Purification Resin from Merck). After several washes with high salt (500 mM NaCl) ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was loaded on to a gravity flow-based Ni-NTA metal affinity column (2 ml beads, cOmplete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... WT—or its mutants fused with a His-tag on the amino-terminus—were expressed in Escherichia coli BL21 (DE3) using pET15b vector (Merck). A single colony was inoculated in 2 mL Luria-Bertani broth (LB ...
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bioRxiv - Microbiology 2021Quote: ... The sonicated cellular extract was spun down and the supernatant has been incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The supernatant was passed through a 0.45 µM syringe filter before transferring into a tube containing HIS-Select Nickel Affinity Gel (Merck India) and kept on a mixer at 4°C for 3 h ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a gravity flow-based Ni-NTA metal affinity column (2 ml beads, complete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Neuroscience 2023Quote: ... R&D, AF2408), Goat-Anti Human SOX17 (1:100, R&D, AF1924) or Mouse Anti-Human Beta-Tubulin (1:1000, Merck, T8660) primary Chicken Anti-Human MAP2 (1:2000 ...
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bioRxiv - Molecular Biology 2023Quote: ... stained with anti-human IFN-γ and anti-human IL2 antibodies for 40 min at RT in 0.2% saponin (Merck, Germany) in PBS (Gibco ...
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bioRxiv - Neuroscience 2020Quote: Recombinant GST-hSLP4A was expressed in and purified from BL21 Rosetta DE3 E.coli (Merck, Ca. No. 70954). 5ml of saturated pDEST15 hSlp4-a-transformed BL21 Rosetta DE3 E.coli culture were diluted in 500 ml 2x YT (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were blocked with 10% (W/V) skim milk (Merck, USA) and subsequently incubated with primary antibodies for 2 h at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... the samples were treated twice with 0.1 % (w/v) thiocarbohydrazide (Merck, 223220) in DIW for each 20 min and 1 h at room temperature in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... grids were colored with aqueous 2% (w/v) uranyl acetate (Merck, France) and then dried with ashless filter paper (VWR ...
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bioRxiv - Microbiology 2019Quote: ... and pneumococcal killing was blocked by protease inhibitor cocktail set V (Merck, Darmstat ...
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bioRxiv - Microbiology 2022Quote: ... 100 mg L−1 hydrogen peroxide (reagent grade 35% w/v, Merck) was added ...
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bioRxiv - Biophysics 2021Quote: ... and/or reactants such as 10% (w/v) 1,6-hexanediol (Merck, Germany), 300 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Sections were stained with 1% (w/v) toluidine blue O (Merck, Germany) in 1% Na2B4O7 for 20 seconds at 50°C ...
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bioRxiv - Physiology 2021Quote: ... supplemented with 1x Protease Inhibitor Cocktail Set V (Merck Life Science, UK). Cell lysates were centrifuged for 15 minutes at 18,000 × g (4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... grids were colored with aqueous 2% (w/v) uranyl acetate (Merck, France) and then dried with ashless filter paper (VWR ...