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Citations for Merck :
251 - 300 of 779 citations for Recombinant Human V erb b2 Erythroblastic Leukemia Viral Oncogene Homolog avian His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 1%(w/v) Triton X-100 (Merck), 2 mM EDTA) ...
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bioRxiv - Cell Biology 2023Quote: ... and v-ATPase (Merck, HPA028701, 1:1000) or Tris-EDTA buffer (10mM Tris Base ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on a 2 ml HIS-SelectTM Nickel Affinity Gel (Merck) and washed with wash buffer ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on a 2 ml HIS-SelectTM Nickel Affinity Gel (Merck) and washed with wash buffer ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore ...
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bioRxiv - Cell Biology 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
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bioRxiv - Biophysics 2023Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosetta 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 µg/ml kanamycin and 34 µg/ml chloramphenicol ...
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bioRxiv - Biophysics 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Molecular Biology 2023Quote: ... or HIS-Select Nickel Affinity Gel in a gravity-flow column (Merck, Darmstadt, Germany), followed by washing with binding buffer with and without 1.3% Triton X-100 ...
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bioRxiv - Biochemistry 2020Quote: ... Flag-tagged proteins were eluted by incubation with 150 μg/mL Flag tag peptide (Merck; F3290) with 0.1% digitonin in solubilisation buffer for 30 min at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... NaOCl and 0.02% (v/v) Triton X-100 for 10 min and washed five times with ultrapure water (Milli-Q; Merck). Following seed stratification at 4°C for 2 d ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibodies were diluted with incubation buffer containing 10% (v/v) normal donkey serum (Merck KGaA, Darmstadt, Germany), 2% bovine serum albumin and 0.5% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... resolubilized in 25 µL of 0.05% trifluoroacetic acid in 98:2 H2O/acetonitrile (v/v) and filtrated through a 0.2 µm spin filter (Merck Millipore Ultrafree®-MC ...
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bioRxiv - Bioengineering 2020Quote: ... and 0.05% (v/v) of an antibiotic and antimycotic mixture (penicillin, amphotericin B, and streptomycin; Merck KGaA, Darmstadt, Germany). As trehalose treatment ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μL of the bacteriocin sample was loaded onto the trap column at 3% (v/v) ACN (Merck, USA) containing 0.1% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Isolated lipids were dissolved in 30 μL of chloroform:methanol (2:1, v/v) and 5 μL loaded on thin-layer chromatography (TLC) silica gel plates F254 (Merck). Lipids were separated in chloroform/methanol/water (20:4:0.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Surfaces were functionalised by flooding the device with 1% (v/v) trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Merck) in HFE-7500™ (3M™) ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed three times with PBS and for 1sec preextracted using PBS supplemented with 0.1% (v/v) Triton-X100 (Merck) to reduce the nucleoplasmic EGFP signals ...
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bioRxiv - Immunology 2020Quote: ... France) in 0.11 mol/L sodium acetate (pH 5.5) containing 0.003% (v/v) H2O2 (all from Merck, Darmstadt, Germany). The reaction was stopped with 2M H2SO4 (Merck) ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... mitochondria-enriched fractions were processed by adding 0.25% (v/v) of SDS-PAGE sample buffer (#PCG3009; Sigma-Aldrich/Merck) to 20 μg of mitochondrial protein and run with Tris-MOPS running buffer (#PCG3003 ...
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bioRxiv - Neuroscience 2022Quote: ... were diluted 1:400 v/v in 0.5% low gelling temperature agarose in Ames’ medium at 37°C (A1420; Merck). A thin film was polymerized over a pure agarose meniscus in a Petri dish and covered with Ames’ medium ...
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bioRxiv - Cell Biology 2022Quote: ... The buffers used during the whole procedure had fish gelatine 1.6% (v/v) as a blocking agent (G7765, Merck). The primary antibodies used were anti-NR1D1 (ab174309 ...
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bioRxiv - Molecular Biology 2022Quote: Cells were harvested and lysed in RIPA buffer (50 mM Tris/HCl pH 7.5, 150 mM NaCl, 1 % (v/v) Triton X-100 (Merck), 0.5 % (w/v ...
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bioRxiv - Microbiology 2023Quote: ... The lower chloroform layer containing the blue pigment pyocyanin was mixed with 0.1 N HCL (20%v/v; Merck). This caused a change of colour from blue to pink ...
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bioRxiv - Cell Biology 2023Quote: ... and were treated with various concentrations of peptides (as determined by prior Hoechst 33258 staining in serum–free media) or solvent (DMSO, 0.1% v/v, Merck) or positive control TGF-β1 10ng/ml (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1% DDM, 10% (v/v) ethylene glycol, 0.005% asolectin (Avanti Polar Lipid, USA) and 0.005% CHAPS (Calbiochem, Merck, Germany), and eluted by an increasing proportion of the same buffer plus 1 M NaCl ...
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bioRxiv - Microbiology 2023Quote: ... each pure culture pellet was re-suspended with corresponding medium supplemented with 20% (v/v) glycerol (Merck, Darmstadt, Germany) and stored at –80°C.
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bioRxiv - Neuroscience 2024Quote: ... All primary and secondary antibodies were diluted in a blocking solution consisting of PBS and 0.2% (v/v) bovine serum albumin (BSA, Merck). Cells were first washed with ice-cold PBS and incubated for 5 min in a blocking solution on ice ...
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bioRxiv - Microbiology 2024Quote: ... The lower chloroform layer containing the blue pigment pyocyanin was mixed with 0.1 N HCL (20%v/v; Merck). This caused a change of colour from blue to pink ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed 4 times for 5 min with 1 x PBS/Tween20 0.1 % (v/v) and the HRP conjugated secondary antibodies (A5420 and A4416, Merck) were added 1:5000 (goat ...
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bioRxiv - Biochemistry 2024Quote: ... Diener Electronic) followed by channel surface functionalization using 1% (v/v) trichloro(1H,1H,2H,2H-perfluorooctyl) silane (Merck) in HFE-7500™ (3M™ Novec™).
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bioRxiv - Cell Biology 2024Quote: ... The immunostainings were performed at RT using tris-buffered saline (TBS) containing 1.6% (v/v) fish gelatine (G7765, Merck) as the basal buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells (5 × 105) were mixed in 2ml viral supernatant supplemented with 8μg/ml polybrene (Merck Millipore) and further incubated at 37°C ...
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bioRxiv - Microbiology 2020Quote: Viral proteins were separated by SDS-PAGE and transferred to polyvinylidene disulfide (PVDF) membranes (Merck Millipore). After blocking the membranes with 5% skim milk ...
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bioRxiv - Microbiology 2022Quote: ... Two days post transfection the viral supernatant was harvested and filtered (0.45 μm pore, Merck Millipore) before transduction of TREx BCBL1-Rta cells in the presence of 8 μg/mL of polybrene (Merck Millipore) ...
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bioRxiv - Cell Biology 2023Quote: ... the viral supernatant was collected and filtered with a 0.45 μm filter unit (Merck Millipore; #051338) and viral aliquots were stored at -80°C until use.
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bioRxiv - Biophysics 2023Quote: ... 2 mL of supernatant containing viral particles was harvested and filtered with 0.45 μm filter (Merck). Supernatant was immediately used for transduction or stored at -80°C in aliquots.
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bioRxiv - Cell Biology 2020Quote: ... the GFP-Trap bead bound fraction was incubated with recombinant TEV (Merck) overnight at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... 100 μl of 10 μg/ml recombinant protein G (Merck, Darmstadt, Germany) was added and incubated for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were treated with recombinant mouse TNFα (10 ng/ml, Merck 654245).
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bioRxiv - Physiology 2021Quote: ... with 0.2 % m/v Triton-X100 and 5% Normal Goat Serum (m/v) (S26-LITER, Merck Life Science UK LTD) (PBST.NGS ...
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bioRxiv - Bioengineering 2020Quote: ... we prepared a 1:1 (v/v) stock solution of MA+ in HBr by adding a methylammonium hydroxide (MAOH) solution (40% in H2O; Merck) dropwise into concentrated HBr ...
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bioRxiv - Plant Biology 2021Quote: ... surface sterilized with 10% (v/v) bleach solution and plated on agar plates containing one-half strength Murashige and Skoog (MS) salts (Merck) pH 5.7 ...
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bioRxiv - Microbiology 2021Quote: ... by high-performance thin-layer chromatography (HPTLC) in CHCl3/MeOH 85:15 v/v on glass-backed 10 × 10 cm silica gel 60 F254 plates (Merck) and a small sidebar of the plate was stained with Hanessian’s stain (0.5 g of cerium(IV ...
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bioRxiv - Developmental Biology 2020Quote: ... for 1 h at 50 V followed by 3.5 h at 100 V and blotted on PVDF-membranes (Immobilon-PSQ Membrane, Merck Millipore). Incorporation of [35S]-methionine into newly synthesized proteins enabled the detection of translation products by phosphor imaging (exposure time of 1 day).
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bioRxiv - Genomics 2019Quote: ... for 1 hour at 50 V followed by 3.5 hours at 100 V and blotted on PVDF-membranes (Immobilon-PSQ Membrane, Merck Millipore). Incorporation of [35S]-methionine into newly synthesized proteins enabled the detection of translation products by phosphor imaging (exposure time of 1 day).
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bioRxiv - Bioengineering 2019Quote: ... 1 mM EDTA) and glycerol (2.5%, v/v) were mixed and polymerized by the addition of fresh 10% APS solution (Merck Millipore) (final concentration 10% ...
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bioRxiv - Microbiology 2019Quote: ... samples were fixed in 2.5% (v/v) glutaraldehyde in HBSS on IsoporeTM membrane TMTP filters with pore size 5 µm (Merck-Millipore) for 30 min followed by washing thrice with HBSS buffer (10 min each) ...
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bioRxiv - Bioengineering 2022Quote: ... were rinsed with PBS and incubated in 500 μL of cell lysis solution containing 0.2% (v/v) Triton-x-100 (Merck, 1.08603.1000) in 5 mM MgCl2 (Sigma-Aldrich ...