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Citations for Merck :
301 - 350 of 779 citations for Recombinant Human V erb b2 Erythroblastic Leukemia Viral Oncogene Homolog avian His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... the wells were rinsed 3 times with PBS and covered with 100 μL 0.5% (v/v) Triton-X 100 (Merck, 1.08603.1000) in PBS for 5 minutes to permeabilize cells ...
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bioRxiv - Microbiology 2022Quote: ... wells were washed twice with warm PBS before incubation in 500 μL pre- warmed DMEM with 1% (v/v) Nutridoma (Merck) supplemented with 5 μM clickable sphingosine (Cayman Chemical ...
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bioRxiv - Molecular Biology 2023Quote: ... for 90 min at 120 V and blotted at constant 30 V for 30 min onto 0.45 µm Immobilon-FL PVDF membrane (Merck Millipore). After the transfer ...
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bioRxiv - Evolutionary Biology 2023Quote: The frozen cell pellet was homogenized in RIPA buffer (Fujifilm, Osaka, Japan) containing 1/100 (v/v) in a final volume of Protease Inhibitor Cocktail (Merck) for 30 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Hydrophobic surface treatment was performed after bonding by flushing with 1% (v/v) trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Merck) in HFE-7500 and ...
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bioRxiv - Bioengineering 2023Quote: ... the wells were rinsed with PBS twice and covered with 100 μL 0.5% (v/v) Triton-X 100 (Merck, 1.08603.1000) in PBS for 5 minutes to permeabilize cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were fixed using formaldehyde (1% V/V in H2O) for 10 min at RT before supplementation with 125 mM glycine solution (Merck) and agitation for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... LB plates were seeded with the different cultures and Whatman n° 1 filter discs (6 mm) were impregnated with 5 μl of 30% (v/v) H2O2 (Merck) as previously described [31] ...
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bioRxiv - Microbiology 2024Quote: ... Samples were separated on a 1.2 % agarose gel stained with 0.01 % (v/v) GelGreen Nucleid Acid Stain (Merck, Darmstadt, Germany) in TBE buffer for 2 h at 120 V ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM DTT and 5%(v/v) glycerol by five cycles of filtration through 10 kDa Amicon filters (Merck Millipore), and finally concentrated to 0.5 mg/ml.
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bioRxiv - Cell Biology 2024Quote: Samples were resuspended in 6 μL 40% (v/v) 1-propanol with vortexing and bath sonication were applied to Silica Gel 60 aluminium-backed HPTLC plates (MERCK) (30 ...
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bioRxiv - Microbiology 2024Quote: ... We stored them at -80 °C in phosphate buffered saline (PBS) with 20 % (v/v) glycerol (anhydrous; Merck, Darmstadt, Germany) and cultivated the isolates on Columbia blood agar plates containing 5 % sheep blood (Mast Diagnostica ...
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bioRxiv - Microbiology 2021Quote: 10His-tagged MA gene was expressed in Escherichia coli (E. coli) strain BL21 (DE3) (Merck, Darmstadt, Germany). The 10His-tagged MA protein was initially purified with nickel-NTA affinity column chromatography followed by TEV cleavage ...
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bioRxiv - Cell Biology 2019Quote: Human fibronectin was from Merck Millipore ...
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bioRxiv - Developmental Biology 2021Quote: ... 5.06% fibrinogen (human plasma; Merck), 3U/mL thrombin (Stago BNL ...
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bioRxiv - Microbiology 2022Quote: ... Plasma-purified human thrombin (Merck) was then added to reactions as required from a 1 IU / μL stock ...
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bioRxiv - Biophysics 2019Quote: ... or 10% (w/v) 1,6-hexanediol (Merck, Germany) were mixed with 10 μM rPrP90-231 in 10 mM Tris (pH 7.4) ...
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bioRxiv - Bioengineering 2021Quote: ... LB consists of 1 % (w/v) NaCl (Merck), 0.5 % (w/v ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.1% (wt/v) NaN3 (Merck, Darmstadt, Germany) on a shaker ...
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bioRxiv - Genomics 2021Quote: ... AC agar with 0.8% gelatin (Merck, w/v), AC agar with 0.8% soluble starch (w/v) ...
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bioRxiv - Cell Biology 2021Quote: ... 10% (w/v) D-glucose (Merck; cat.# 104074), 0.5 mg/mL glucose oxidase (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2023Quote: ... and 10% (w/v) Dextran Sulfate (Merck, S4030) 23 on a Hybrislip at 37°C overnight in a humidified chamber ...
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bioRxiv - Cell Biology 2023Quote: ... + 0.2 % (w/v) Bovine Serum Albumin (Merck, A3059)) ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.05% w/v dextran sulfate (Merck D4911) and Image-iT FX reagent (Invitrogen I36933 ...
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bioRxiv - Cancer Biology 2020Quote: ... Viral pellets were resuspended in culture medium supplemented with 8 μg/ml Polybrene (Merck, TR-1003-G) and 10 μM Y-27632 (Selleckchem ...
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bioRxiv - Neuroscience 2020Quote: ... culture medium was collected and viral particles were enriched by filtration (Amicon Ultra-15 Centrifuge Filters, Merck). Titration of every batch of lentiviral preparation was estimated following transduction of HEK293T cells ...
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bioRxiv - Cell Biology 2020Quote: ... Viral particles were purified on iodixanol gradients and concentrated on Amicon Ultra-15 100K columns (Merck-Millipore). The concentration of viral genomes (vg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Viral particles solution was collected and ultrafiltered with Amicon Ultra-15 centrifugal filters (100 kDa, Merck, Germany) to obtain the pure AAVs solution for animal administration.
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bioRxiv - Biochemistry 2020Quote: ... cells were washed 3x in PBS followed by blocking in 16% (v/v) normal goat serum (Merck Milipore, Burlington, MA, USA) supplemented with 0.1% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... for 90 min at 120 V and blotted at constant 30 V for 30 min onto Immobilon-FL PVDF membrane (Merck Millipore). After the transfer ...
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bioRxiv - Bioengineering 2022Quote: ... was used to mimic tissue-like scattering conditions and Nigrosin (0.62 v/v% of Nigrosin stock solution [0.5 mg/mL Nigrosin in deionised water, Merck, 8005-03-6]) was added to mimic tissue-like absorption.
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bioRxiv - Synthetic Biology 2023Quote: ... The routinely used growth medium was composed of yeast extract (1%(w/v)) (Fisher BioReagents™) and casein peptone (2%(w/v)) (Merck), and was supplemented with 2% (w/v ...
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bioRxiv - Bioengineering 2023Quote: ... samples were incubated in a 10% (v/v) HS PBS solution supplemented with primary mouse anti-β-tubulin III antibody (1:100; Merck) overnight at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% (v/v) fetal bovine serum, 1 mM sodium pyruvate (gibco, 11360039) and 1% antibiotic/antimycotic solution (Merck, A5955). Medium was refreshed every 3 days during cultivation and every 24h in an experimental setting ...
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bioRxiv - Genomics 2022Quote: ... During sampling we set a flow rate of 200 l/minute for a total of 60 minutes with 10 ml 0.05 % (v/v) Triton X-100 (T8787-50ML, Merck Sigma Aldrich) as collection buffer prepared with UltraPure DNase/RNase free ...
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bioRxiv - Developmental Biology 2023Quote: Cells were homogenized in 20 mM Tris-HCl buffer (pH 7.2, 0.2 mM EGTA, 5 mM β-mercaptoethanol, 2% (v/v) antiprotease cocktail (Merck KGaA, Germany), 1 mM PMSF ...
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bioRxiv - Immunology 2023Quote: ... A549 cell were incubated with lentiviral supernatants diluted 1:20 (v/v) in culture medium containing 10 µg/ml polybrene (Merck Millipore) and centrifuged at 805xg at 20 °C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting concentrate was applied to a 10 - 50% v/v iodixanol step gradient with 10% increments (OptiPrep; Sigma Aldrich, Merck) enriched with sea salts (Sigma Aldrich ...
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bioRxiv - Plant Biology 2023Quote: Plants were grown on 1/2 MS agar plates containing 0.01% (v/v) dimethyl sulfoxide (mock) or 50 ng mL-1 TM (654380; Merck, Darmstadt, Germany) for 14 days ...
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bioRxiv - Microbiology 2023Quote: ... 10% (v/v) glycerol using an Amicon Ultra-4 centrifugal column with a 10 kDa molecular weight cut-off (Merck-Millipore). This process yielded a protein concentrated to ∼10–20 mg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... Protein G Dynabeads (catalogue # 10003D) were washed with 0.02% (v/v) PBS-Tween 20 (Tween-20: catalogue # P2285, Merck; 0.01M PBS) and incubated with 200µL of primary antibody mix (for antibody details see Supplementary Table 5 ...
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bioRxiv - Genetics 2021Quote: Before the ovaries were stimulated with recombinant FSH (Gonal-F, Merck Serono, Switzerland), downregulation was performed using a GnRH agonist (Decapeptyl ...
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bioRxiv - Zoology 2020Quote: ... Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck) in TE buffer (10 mM Tris-HCI ...
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bioRxiv - Cell Biology 2022Quote: All experiments were performed using the BDNF mature recombinant (GF029; Merck Millipore, USA) or the C-ter truncated form of BDNF (BDNFΔC-ter) ...
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bioRxiv - Plant Biology 2022Quote: ... The filtered supernatant was loaded onto a column packed with cOmpleteTM His-tag purification resin (Merck). His6-proteins were eluted with 4x 2 bead volumes of elution buffer (20 mM Na HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... StrepII-tagged proteins (RBBP6, symplekin and WDR33) were detected using streptavidin-HRP conjugate (Merck Millipore, cat. No. 18152) and Amershan ECL Detection Reagents (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... The expression of S-tagged NrdA was confirmed by purification using S-protein agarose (Merck Millipore, Darmstadt, Germany) as previously described (11 ...
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bioRxiv - Cell Biology 2020Quote: ... Purification of His6-tagged proteins from the soluble fraction was achieved using Ni-NTA His.Bind® Resin (Merck), a peristaltic pump and a series of column washes with buffers of increasing imidazole content ...
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bioRxiv - Evolutionary Biology 2020Quote: ... F2 FLAG-tagged TBP::NveRLR::mCherry and wild-type zygotes were treated with 3% L-Cysteine (Merck Millipore), washed and snap frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2020Quote: ... to obtain a final concentration of 80 % acetone (v/v) and pipetted on an Ultrafree-MC PTFE membrane 0.2 µm filter (Merck Millipore, Burlington, MA). The filter was incubated for 2 min at room temperature and was then centrifuged at 5000 x g for 1 min ...