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Citations for Merck :
351 - 400 of 522 citations for Siglec 15 Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Samples were separated on a 4–15% gel by SDS-PAGE and transferred to PVDF-FL membranes (IPFL00010, Merck). Membranes were blocked with 5% non-fat milk in PBS and incubated with primary antibodies diluted in 5% non-fat milk in PBS+0.1%Tween-20 (PBS-T ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 µL lysis buffer (15 mM NaHEPES pH 7.3, 30 mM NaCl, 1 mM MgCl2, 1% Brij35, 1 U/µL benzonase (Merck), 1X complete protease inhibitor (EDTA-free ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated and using using Amicon Ultra-15 centrifugal filters (3 kDa cut-off, Merck-Millipore) and then buffer was exchanged to buffer B (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: The aqueous solutions of GQDs were purified utilizing dialysis kits and Amicon ultra-filtration devices (Amicon Ultra-15, Merck) with 1 and 3 kDa membranes ...
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bioRxiv - Biophysics 2023Quote: ... Protein extracts (50 μg/lane) were resolved in 15% SDS-PAGE followed by immunoblotting using anti-GFP (AB3080P, Merck), anti-PrPC monoclonal antibody (anti-CD320 clone 6D11 ...
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bioRxiv - Physiology 2023Quote: ... For citrate synthase activity, 15-30 µg protein was added to the reaction buffer (20 mM Tris-HCl, pH 8.0 (Merck), 0.42 mM acetyl-coenzyme A (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples (5 to 25µg/lane) were separated by 12.5% – 15% SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Merck Millipore). Proteins were probed in Can Get Signal Solution1 (Toyobo ...
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bioRxiv - Bioengineering 2023Quote: ... Medium was further concentrated to volumes <1 mL using 100kDa MWCO Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore). Tricorn 10/300 chromatography columns (Cytiva ...
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bioRxiv - Biophysics 2021Quote: ... The solution was concentrated with an Amicon Ultra-15 Centrifugal Filter (NMWL of 10 kDa, Merck Millipore, Burlington, MA, USA) and loaded into a Superdex75 10/300 GL gel filtration column (GE Health-care Life Sciences ...
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bioRxiv - Evolutionary Biology 2020Quote: ... at 4°C in dialysis buffer (100 mM NaCl, 20mM Tris pH 7.4, containing 15 μl bovine thrombin (605157, Merck Millipore) to cleave the His-tags) ...
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bioRxiv - Cell Biology 2020Quote: ... Media was exchanged with YPAD containing α-factor and grown for an additional 2 h and 15 min Cells were then released into the cell cycle by washing twice with YPAD and adding Pronase E (Merck). Nocodazole (VWR ...
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bioRxiv - Physiology 2022Quote: ... frozen fresh tissue (∼20-45 mg) was placed into a 2 ml sterile microcentrifuge tube pre-loaded with ∼15-20 glass beads (Merck) while 200 μL of ice-cold methanol (Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... The proteins were again diluted in 9 volumes of Exchange Buffer 2 (Exchange Buffer 1 with 200 mM NaCl) and concentrated with 30 MWCO Amicon Ultra-15 (Merck).
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bioRxiv - Physiology 2020Quote: ... Fusion proteins were exchanged into low (< 30 mM) imidazole buffer using an Amicon Ultra-15 centrifugal concentrators (Merck Millipore, Germany) with a 30 kDa molecular weight cut-off in preparation for liberation of Hi1a from the fusion tag using tobacco etch virus (TEV ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant protein was dialyzed overnight using an Amicon Ultra 15 mL spin dialysis column 10 000 MWCO (Merck, Germany). Purified protein was quantified using Bradford’s assay ...
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bioRxiv - Biochemistry 2021Quote: ... 100 mM EDTA or 200 mM Imidazole) and concentrated up to 0.5 ml using Amicon Ultra-15 centrifugal filters with MWCO of 50 kDa (Merck, Germany). The sample was then applied to a size exclusion chromatography on a Superdex 200 increase 10 /300GL column equilibrated with wash buffer ...
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bioRxiv - Biophysics 2021Quote: ... The eluted sample was exchanged into 20 mM Tris buffer and concentrated to 5 ml by Amicon Ultra-15 3 kDa (Merck). The sample was purified by 320 ml of HiLoad Superdex with a flow rate of 1 ml/min using the FPLC systems.
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bioRxiv - Biochemistry 2021Quote: ... Proteins were further separated by size exclusion chromatography (HiLoad 26/600 Superdex 75, Cytiva) and concentrated using Ultra-4 or 15 mL centrifugal filter devices (Amicon, Merck). Correct size and high purity were verified via SDS-PAGE and LC-MS analysis ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Major peaks were desalted by RP-HPLC using an Ascentis C18 column (3 μm, 4.6mm ID x 15 cm, Sigma-Merck, Germany) using peak-based collection (slope) ...
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bioRxiv - Plant Biology 2021Quote: ... Purified ClpP complexes were concentrated to ~2 mg/ml by Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore, Beijing China) with 100 kDa cut-off ...
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bioRxiv - Microbiology 2021Quote: ... by high-performance thin-layer chromatography (HPTLC) in CHCl3/MeOH 85:15 v/v on glass-backed 10 × 10 cm silica gel 60 F254 plates (Merck) and a small sidebar of the plate was stained with Hanessian’s stain (0.5 g of cerium(IV ...
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bioRxiv - Molecular Biology 2021Quote: ... and eluted into PBS containing 250 mM imidazole and further concentrated and desalted with an Amicon Ultra-15 Centrifugal Filter Device (Merck). The Ni-NTA affinity chromatography purified eLACCO1 protein was further applied on Superdex200pg (GE healthcare ...
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bioRxiv - Microbiology 2020Quote: ... all supernatant was removed and the cells were fixed with 10% formalin for 15 min at room temperature and stained with 100 µg/ml Hoechst 33342 (Merck) for 30 min in the dark at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The eluate was neutralised immediately using 1.5 M Tris pH 8.6 and then buffer exchanged to PBS using a 15 ml 30 kDa MWCO centrifugal filter (Merck Millipore).
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bioRxiv - Biophysics 2020Quote: ... 50 μL of sample was injected onto a Discovery BIO Wide Pore C18 column (15 cm x 4.6 mm, 5 μm column with a guard column) (Supelco, Merck, UK) and eluted on a gradient of 95% water + 0.1% acetic acid and 5% acetonitrile + 0.1% acetic acid to 5% water + 0.1% acetic acid and 95% acetonitrile + 0.1% acetic acid at a 0.8 mL/min flow-rate over 40 mins ...
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bioRxiv - Biochemistry 2022Quote: ... Elution fractions containing MbxA were pooled and concentrated with Amicon Ultra-15 Centrifugal Filter Units (50 kDa NMWL, Merck Millipore). Histidine was removed with a PD10 desalting column (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... The elution volume of 60-70 mL was collected and the solution was concentrated to a volume of 200 μL with an Amicon Ultra-15 PLHK Ultracel-PL Membrane 100 kDa (Merck) spin column ...
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bioRxiv - Genetics 2021Quote: ... At day 10 cells were passaged at a 2:3 ratio into 12 well cell culture plates coated with 15 µg/ml human plasma fibronectin (Merck) in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Microbiology 2021Quote: ... lysed in Laemmli SDS-loading dye and separated in 15% acrylamide gels and transferred to 0.45 μm hydrophobic Immobilon-P PVDF membrane (Merck Millipore). Immunoblotting was as described previously 19 ...
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bioRxiv - Biophysics 2021Quote: ... and buffer exchanged into 50 mM trisaminomethane-hydrochloride (Tris-HCl) at pH 7.4 using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore). The A90C variant was labelled with 1.5-fold molar excess of C5 maleimide-linked Alexa Fluor 647 (Invitrogen Life Technologies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... while the rat abdominal aortic rings (15 mg wet mass/mL) were pre-incubated for 10 min in Medium 199 (Merck). The examined enzyme reaction started after pipetting the tracer substrate 1-14C-AA (3.7 kBq ...
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bioRxiv - Synthetic Biology 2022Quote: ... Dialyzed samples were further concentrated to 10 mg mL-1 using Amicon® Ultra-15 Centrifugal Filter Unit (Merck Millipore). The final concentration of glycerol was adjusted to 50% (v/v% ...
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bioRxiv - Biochemistry 2022Quote: ... for 30 min at room temperature – was diluted to ∼0.002 mM DTT by three rounds of dilution/concentration in an Amicon Ultra 15 mL 3K Centrifugal Filter (Merck Millipore). TEV protease cleavage was performed overnight at room temperature.
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bioRxiv - Microbiology 2019Quote: ... pH 7.4) through multiple dilution and centrifugation steps using Amicon Ultra-15 centrifugal filters (10 kDa cuttoff, Merck Millipore, Germany). Protein purity was estimated with SDS-PAGE and the concentration determined with the Pierce BCA assay kit (Thermo Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... Fractions containing protein were pooled and concentrated using Amicon Ultra-15 centrifugal filter unit (10-kDa molecular mass cut-off; Merck) until total protein concentration of 10 mg·mL−1 was reached ...
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bioRxiv - Biophysics 2019Quote: ... Peak fractions monitored by SDS-PAGE were collected and exchanged with the storage buffer using AmiconUltra-15 10k and AmiconUltra-0.5 10k filters (Merck Millipore). Samples were stored at −80°C ...
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bioRxiv - Neuroscience 2020Quote: ... culture medium was collected and viral particles were enriched by filter device (Amicon Ultra-15 Centrifuge Filters, Merck, Cat#UFC910008). Titer check was performed on HEK293T cell culture for every batch of lentiviral preparation.
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bioRxiv - Neuroscience 2020Quote: ... culture medium was collected and viral particles were enriched by filter device (Amicon Ultra-15 Centrifuge Filters, Merck, Cat#UFC910008). Titer check was performed on HEK293T cell culture for every batch of lentiviral preparation.
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bioRxiv - Biochemistry 2021Quote: ... the samples were filtered with Sterile Syringe filter (0.22 μM) and concentrated with Millipore Amicon Ultra-15 10K (Merck Millipore) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... were mixed with 120 μL of the TBA-TCA reagent (0.038% thiobarbituric acid (Kemika, Croatia) in 15% trichloroacetic acid (Merck, USA). The samples were diluted with 70 μL of ddH2O ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein was collected from the supernatant of cultured cells and concentrated by Amicon Ultra-15 Centrifugal Filter Unit (Merck). Then the fusion protein was purified using BeaverBeads IDA-Nickel Kit (Beaverbio) ...
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bioRxiv - Molecular Biology 2022Quote: ... Peak fractions were pooled and concentrated using a 30 kDa cut-off Amicon Ultra-15 concentrator (Merck, cat No. UFC903024) to a typical concentration of about 6 mg/ml ...
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bioRxiv - Biophysics 2022Quote: ... Fraction 4-6 of three different columns were pooled and concentrated to 0.5 mL using an Amicon Ultra-15 10K filter (Merck Millipore). This was then placed on top of the gradient.
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bioRxiv - Microbiology 2022Quote: ... ZIKV was concentrated using an Amicon Ultra-15 Centrifugal Filter Unit with an Ultracel-100 membrane (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Physiology 2022Quote: ... They were then permeabilized with 0.02% Triton X-100 in PBS for 15 min and blocked with 5% bovine serum albumin (Sigma-Aldrich, Merck) in PBS for 3 h ...
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bioRxiv - Neuroscience 2023Quote: ... culture medium was collected and viral particles were enriched by filter device (Amicon Ultra-15 Centrifuge Filters, Merck, Cat#UFC910008). Titer check was performed on HEK-293T cell culture for every batch of lentiviral preparation.
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bioRxiv - Molecular Biology 2023Quote: ... HeLa cells grown on 15 cm plates (∼90% confluent) were treated either with 100 μg/mL cycloheximide (CHX, Merck, C1988) for 15 min or with 50 μg/mL puromycin (Puro ...
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bioRxiv - Microbiology 2023Quote: ... The volume of concentrated viral fractions (>50 kDa) was further reduced using Amicon Ultra 15 ml Centrifugal Filters (Merck-Millipore) prior to phenol/chloroform extraction ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was subjected to three freeze-thaw cycles and incubated with SAN (Merck, 50 U per 15 cm dish) for 1 h at 37 C ...
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bioRxiv - Immunology 2023Quote: ... Fractions with CPC-activated PO activity were pooled and concentrated to 1.1 ml using AmiconTM Ultra-15 30K centrifugal units (Merck Millipore). Proteins were separated by Sephacryl S-300 column (Cytiva) ...