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Citations for Merck :
151 - 200 of 522 citations for Siglec 15 Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Fractions with rSpike were concentrated using Amicon Ultra-15 Centrifugal filters (Merck Millipore) with a 3 kDa membrane cutoff and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare Life Sciences ...
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bioRxiv - Synthetic Biology 2022Quote: ... 10% glycerol) using a 10-MWCO Amicon Ultra-15 centrifugal filter unit (Merck). The concentration of the protein was determined with a Bradford assay.
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bioRxiv - Immunology 2023Quote: ... permeabilised for 15 min with 0.5% NP-40 (Merck Life Science, Dorset, UK) then blocked for 1 h in PBS containing 0.1% Tween (PBST ...
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bioRxiv - Neuroscience 2023Quote: ... The eluate was concentrated with Amicon Ultra-15 (Merck Millipore, MWCO 3 K) to a protein concentration of 60 µM.
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bioRxiv - Biophysics 2023Quote: ... concentrated using an Amicon-15 Ultra centrifugal filtration device (10 k MWCO, Merck) and frozen in liquid nitrogen for storage at -80 °C.
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bioRxiv - Biophysics 2023Quote: ... α-synuclein was concentrated using Amicon Ultra-15 centrifugal filter units (Merck Millipore). The protein was subsequently labelled with a 1.5-fold molar excess of C5 maleimide-linked Alexa Fluor 647 (Invitrogen Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Amicon Ultra-15 Centrifugal Filter Units with a 50 kDa cutoff (Merck Milipore) were utilized ...
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bioRxiv - Biochemistry 2022Quote: ... The His6- tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with 2mL Ni-NTA His•Bind® Resin (Merck), followed by incubation with TEV protease for 12 hours at 4 °C to remove the His6 tag ...
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bioRxiv - Microbiology 2021Quote: ... The binding of CoVHH1-FLAG to S1 subunits-His tag was detected by incubating for 1 hour at room temperature with mouse monoclonal ANTI-FLAG® M2-Peroxidase (Merck) diluted with PBST ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Biochemistry 2023Quote: ... The 100-fold dilution of culture was made up to 10-4 dilutions and spotted on TYE plates (Hi-Media Laboratories Ltd., India) having 1% glucose (Merck & Co., USA) and ampicillin (Gold Biotechnology ...
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bioRxiv - Immunology 2023Quote: ... Lysate was clarified by spinning cells for 10 min at 20,000 × g before loading supernatant onto cOmplete™ His-Tag purification resin (Merck; cat # 5893682001). Bound proteins were washed with 20 mM tris (pH 8.0) ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were concentrated with an Amicon Ultra-15 10 kDa centrifugal filter unit (Merck). Protein concentration was determined by UV-spectroscopy.
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bioRxiv - Biophysics 2019Quote: ... an Amicon Ultra-15 Centrifugal Filter with a NMWL of 10 kDa (Merck Millipore) was used ...
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bioRxiv - Neuroscience 2021Quote: ... and lysed directly in 15 µl 2x Laemmli buffer containing 10% 2-mercaptoethanol (Merck). Samples were loaded on a 4-to-20% SDS polyacrylamide gel (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... The purified protein was desalted using the Amicon Ultra-15 Centrifugal Filter Unit (Merck) and the desalted protein was used for activity assays ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 30 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... 0.15 M NaCl) using Amicon Ultra-15 spin columns with 30 kDA cutoff (Merck). To remove the residual nucleic acids bound to NS3H ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 100 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 30 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were further concentrated using Amicon Ultra-15 Centrifugal Filter Units (MERCK, Carrigtohill, Ireland) and stored at -20°C ...
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bioRxiv - Immunology 2020Quote: ... Elutes were buffer exchanged to PBS using Amicon Ultra-15 Centrifugal Filter Units (Merck) following filtration in 0.22μm filter unit (Millex).
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bioRxiv - Molecular Biology 2021Quote: ... and concentrated using Amicon Ultra-15 30 kDa centrifugal filter device (Merck Millipore, #UFC903024) at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mM DTT) using a 10 kDa cut-off Amicon Ultra-15 concentrator (Merck), and frozen at –80 °C until use ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was concentrated by Amicon Ultra-15 Centrifugal Filters (10 KDa) (UFC901008, Merck) and diluted in buffer HT with 50 mM KCl for decreasing of KCl concentration to 90 mM ...
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bioRxiv - Neuroscience 2020Quote: ... followed by desalting and concentration using Amicon Ultra-15 100K Centrifugal Filter Units (Merck). Viruses were aliquoted and stored at -80°C ...
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bioRxiv - Microbiology 2020Quote: ... HEK 293T cells grown on 15-cm dishes were transfected using polyethylenimine (PEI) (Merck), a transfection reagent for standard triple transfection protocol ...
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bioRxiv - Immunology 2021Quote: ... Supernatant were concentrated with Amicon® Ultra–15 Ultrace–30K centrifugal filter unit (MERCK). Cell protein contents were extracted with a lysis buffer composed of 50 mm sodium phosphate ...
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bioRxiv - Microbiology 2022Quote: ... Viral supernatant was concentrated in Amicon ® Ultra-15 Centrifugal Filter units (Merck, Germany) and viral titre determined by the viral immunoplaque assay ...
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bioRxiv - Microbiology 2022Quote: ... The resulting supernatant was ultra-filtered using an Amicon Ultra-15 (Merck, Darmsthadt, Germany) and washed with phosphate-buffered saline (PBS ...
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bioRxiv - Physiology 2023Quote: ... and then concentrated using an Amicon Ultra-15 filter unit (Merck KGaA, Darmstadt, Germany). After lipoprotein fractionation of the concentrated media using FPLC ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... solution for 10 minutes followed tap water for 15 minutes and eosin (Merck, 102439) staining for 10 minutes followed by the dehydration and mounting as above.
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bioRxiv - Microbiology 2023Quote: ... pH 7.5) using Amicon® Ultra-15 centrifugal filter unit (Merck KGaA, Darmstadt, Germany) with a 30K molecular weight cut-off ...
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bioRxiv - Bioengineering 2023Quote: ... and the concentrations were adjusted using Amicon® Ultra-15 Centrifugal Filter Units (Merck) with the appropriate molecular weight cut-off ...
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bioRxiv - Cell Biology 2023Quote: ... for 15 minutes and then blocked with 5% skimmed milk (Merck Millipore, Cat #: 70166) in TBST for 3 hours ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The eluate was concentrated to 500 μL using an Amicon Ultra-15 (Merck Millipore) and further purified by size-exclusion chromatography on a Superdex 200 10/300 column (GE Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... Biofilm samples (volume = 15 mL) were filtered using Millipore Express PLUS Membranes (Merck, Belgium) and Polycarbonate syringe filter holder (Sartorius ...
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bioRxiv - Microbiology 2023Quote: ... concentrated by ultrafiltration in a Amicon Ultra-15 (10 or 30kDa exclusion) (Merck Millipore) and further purified by gel filtration using Superdex 200 column (GE Healthcare) ...
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bioRxiv - Plant Biology 2024Quote: ... A Chromoltih RP-18R 15 cm length 0.1 cm inner diameter column (Merck, Germany) was used.
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bioRxiv - Molecular Biology 2024Quote: ... 300 mM Glutathione) and concentrated using Amicon Ultra-15 at 4000 g (Merck Millipore).
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bioRxiv - Molecular Biology 2024Quote: ... and the concentrations were adjusted using Amicon® Ultra-15 Centrifugal Filter Units (Merck) with 100 kDa cut-off ...
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bioRxiv - Microbiology 2024Quote: ... concentrated up to 5 ml using Amicon Ultra-15 centrifugal filter unit (Merck Millipore) and loaded on a HiLoad 16/600 Superdex 200 gel filtration column (Cytiva ...
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bioRxiv - Bioengineering 2021Quote: Three sender area squares (3 × 3 mm) were put into a 24-well containing 300 μL of anti-His antibody (Merck, Germany, NOVG70796-3) (10 μg mL−1 in DPBS ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were concentrated with Amicon Ultra-15 Ultracel 10k centrifugal filter units (Merck Millipore, #UFC901024). Purification of cytoplasmic actin ...
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bioRxiv - Neuroscience 2021Quote: ... and this solution was ultrafiltered with an Amicon Ultra-15 device (100 kDa, Merck, Germany) to obtain the AAV stock solution for administration to mice.
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bioRxiv - Bioengineering 2022Quote: ... The Netherlands) and concentrated using ultrafiltration (Amicon® Ultra 15 mL Centrifugal Filters, Merck KGaA). Protein concentrations were determined with the BCA protein assay kit (Novagen ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos at stage 15 were incubated with DMSO or Rho Kinase Inhibitor III (555553, Merck) at 250 μM dissolved in water for 2 h ...
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bioRxiv - Biochemistry 2020Quote: ... followed by concentration using Amicon Ultra-15 Centrifugal Filter Unit (100 kDa cutoff, Merck KGaA). The concentrated medium (6 mL ...
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bioRxiv - Biochemistry 2021Quote: ... 9- 15- or 25-mer RNA (Integrated DNA Technologies) or 2.5 mM yeast tRNA (Merck) in the same buffer were added stepwise ...