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Citations for Merck :
101 - 150 of 522 citations for Siglec 15 Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... The CMs were concentrated with Amicon Ultra-15 centrifuge tubes (Merck Millipore Ltd. ...
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bioRxiv - Genomics 2023Quote: ... supplemented with 15% EmbryoMax fetal bovine serum (ES-FBS Merck-Millipore) and 20 ng/ml leukemia inhibitory factor (LIF ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... rh-HI was prepared in either 20% acetic acid (v/v from acetic acid glacial, Merck KGaA, Darmstadt, Germany), 0.3 M NaCl (Merck KGaA ...
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (10,000 x g, 30 min, RT) and incubated for 1 hour with Ni-NTA His bind resin (Merck) rolling at RT ...
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bioRxiv - Biochemistry 2020Quote: ... as matrix followed by ultrafiltration (Amicon Ultra-15 centrifugal filter units, Merck).
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bioRxiv - Molecular Biology 2019Quote: ... 2.5mM L-Aspartate) and concentrating with 30 MWCO Amicon Ultra-15 (Merck) to the starting volume ...
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bioRxiv - Microbiology 2021Quote: ... the clarified filtrate was concentrated using Amicon Ultra-15 centrifugal filters (Merck) with a molecular mass cut-off (MWCO ...
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bioRxiv - Microbiology 2021Quote: ... clarified and concentrated using Amicon Ultra-15 Centrifugal units 30K (Merck Millipore). Virus titers were quantified by plaque assay in Vero-E6.
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bioRxiv - Cancer Biology 2020Quote: ... lysed during 15 min shaking in CelLytic M lysis buffer (Merck KGaA) with a protease inhibitor cocktail and phosphatase inhibitor cocktail (both from Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... fibulin1C was concentrated 15-fold using a Lab scale TFF system (Merck) and purified using a Ni-sepharose column (GE Healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... while the Amicon®Ultra-15 10K (Merck Millipore Ltd., Carrigtwohill, Ireland) filter tubes were used to recover hSDF-1 ...
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bioRxiv - Biophysics 2021Quote: ... proteins were separated from salts and concentrated using Amicon Ultra 15 (Merck). Full length DnaK chaperone was purified by Ni2+-NTA column ...
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bioRxiv - Cell Biology 2022Quote: ... Calcium free or calcium high solutions contained either 15 mM EGTA (Merck) or 25 mM CaCl2 ...
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bioRxiv - Genetics 2022Quote: ... Virus was concentrated using Amcion Ultra-15 30k centrifugal filter (Merck#C7715) and centrifugation for 30 min at 3,900rpm.
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bioRxiv - Molecular Biology 2022Quote: ... Buffer exchange was performed using Amicon Ultra-15 centrifugal filter units (Merck) to have the purified proteins in mTOR wash buffer containing 25 mM HEPES (pH 7.4) ...
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bioRxiv - Immunology 2023Quote: ... (30kDa MWCO - 15 mL sample volume) according to vendor’s instructions (Merck Millipore). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... concentrated using 100 kDa Amicon® Ultra-15 Centrifugal Filter Units (Merck) and cryopreserved at –80°C ...
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bioRxiv - Microbiology 2023Quote: ... infected macrophages were lysed for 15 min with 0.05% IGEPAL solution (Merck) after 3 h of internalization (day 0 ...
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bioRxiv - Biochemistry 2024Quote: ... concentrated using an Amicon Ultra-15 centrifugal filter unit (Merck, Billerica, MA), and applied to a G-25 spin column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... concentrated using an Amicon Ultra-15 centrifugal filter unit (Merck, Billerica, MA), and applied to a PD10 spin column (BD ...
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bioRxiv - Microbiology 2024Quote: ... for 15 min and incubated with anti-Flag M2 affinity beads (Merck) for 1.5 hours at 4°C on a turning wheel ...
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bioRxiv - Biochemistry 2024Quote: ... The eluate was concentrated using an Amicon Ultra-15 concentrator (Merck, UFC903024) and further purified by gel filtration using a HiLoad 16/600 Superdex 200 pg column equilibrated in MCM buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... and applied directly onto a column of 2 ml pre-equilibrated Ni-NTA beads (cOmplete His-Tag Purification Resin from Merck). After several washes with high salt (500 mM NaCl) ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was loaded on to a gravity flow-based Ni-NTA metal affinity column (2 ml beads, cOmplete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt (50mM Tris pH8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt buffer (50mM Tris pH7.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... WT—or its mutants fused with a His-tag on the amino-terminus—were expressed in Escherichia coli BL21 (DE3) using pET15b vector (Merck). A single colony was inoculated in 2 mL Luria-Bertani broth (LB ...
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bioRxiv - Microbiology 2021Quote: ... The sonicated cellular extract was spun down and the supernatant has been incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged Trx1 was purified on a Ni2+ sepharose resin following the manufactureŕs instructions (cOmplete™ His-Tag Purification Resin, Merck).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The supernatant was passed through a 0.45 µM syringe filter before transferring into a tube containing HIS-Select Nickel Affinity Gel (Merck India) and kept on a mixer at 4°C for 3 h ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a gravity flow-based Ni-NTA metal affinity column (2 ml beads, complete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Immunology 2021Quote: Infected red blood cells (iRBC) were stored with 15 % glycerol (Merck, Darmstadt, Germany), 2.1 % sorbitol (Sigma Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... The fractions containing ubiquitin were concentrated by Amicon Ultra-15 3 kDa (Merck) and then loaded onto a HiLoad 16/60 Superdex 75 column (GE Healthcare Life Science ...
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bioRxiv - Physiology 2021Quote: ... Virus was concentrated in PBS using Amicon Ultra-15 Centrifugal Filter Units (Merck) and stored at 4°C.31,77,78 Viral titre was quantified by inverted terminal repeat probe quantitative polymerase chain reaction (PCR) ...
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bioRxiv - Physiology 2021Quote: ... The supernatant was then concentrated on Amicon Ultra 15 filter columns (Merck Millipore). The obtained virus stock was topped up with FBS to 10% and sodium bicarbonate added ...
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Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assembliesbioRxiv - Molecular Biology 2020Quote: ... the protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore). A90C α-synuclein was labelled with 1.5-fold molar excess Alexa Fluor 488 C5 maleimide (Life Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... and then concentrated with an Amicon Ultra-15 centrifugal filter (Merck Millipore, Germany). The concentrated sample was subjected to buffer exchange by dialysis against 20mM Tris-HCl pH7,4.
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bioRxiv - Cell Biology 2021Quote: ... in D-PBS for 15 min and permeabilized with 0.05% saponin (Merck, 47036) in D-PBS supplemented with 1% bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2022Quote: ... eluted NS3H was concentrated using Amicon Ultra-15 with 30 kDA cutoff (Merck) spin columns and loaded into a Superdex 200 Increase 10/300 GL (Cytiva ...
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bioRxiv - Bioengineering 2022Quote: ... the resulting solution was concentrated to 15-25μl using either 100K (Merck – UFC5100) or 10K (Merck – UFC5010 ...
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bioRxiv - Biochemistry 2022Quote: ... The purified proteins were then concentrated using AmiconTM Ultra 15 concentrators (MERCK Millipore) and stored in -80°C.
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bioRxiv - Cell Biology 2019Quote: ... Eluted protein was concentrated using Amicon Ultra 15 centrifugal filters (Merck, Darmstadt, Germany) and separated on a Superose 6 Increase column (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... supernatant was filtered and added into an Amicon Ultra 15 ml tube (Merck) with a 3 kDa cut-off ...
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bioRxiv - Developmental Biology 2019Quote: ... washed again 3 times 15 min and developed using Millipore Crescendo ECL (Merck). For western blot quantification ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... and buffer-exchanged using the Amicon Ultra-15 Centrifugal Filter Units (Merck, Germany) with a 100 kDa molecular weight cut-off in HFB buffer ...
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bioRxiv - Microbiology 2019Quote: ... Filtered supernatants were concentrated to 1 ml using an Amicon Ultra-15 (Merck) with a 100 kDa molecular weight cut off (MWCO ...
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bioRxiv - Immunology 2021Quote: ... or by ultrafiltration using Amicon Ultra 15 (100 kDa; Merck-Millipore, Cork, Ireland).