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Citations for Merck :
301 - 350 of 522 citations for Siglec 15 Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... 150 mM GluK and 5 mM GluMg] using AmiconUltra-15 3k and AmiconUltra-0.5 3k filters (Merck Millipore). The sample was stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... the solution was exchanged with phosphate buffered saline (PBS) using Amicon Ultra-15 10K Centrifugal Filter Devices (Merck). Purity was confirmed by the absence of contamination bands on SDS-PAGE.
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bioRxiv - Neuroscience 2021Quote: ... 15 min) followed by filtering of the supernatant with a 0.45 μm Millex PVDF membrane filter (Merck Millipore). For subsequent FPLC purification (Fast Protein Liquid Chromatography ...
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bioRxiv - Neuroscience 2021Quote: ... The eluates containing viral capsids were collected and transferred to an Amicon Ultra-15 filter tube (Merck Millipore). The filter tube was centrifuged at 3000 x g at 4 °C until less than 1 mL solution was left and the samples in the filter tube were subsequently washed twice with 13 mL PBS ...
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bioRxiv - Neuroscience 2020Quote: ... media were harvested and concentrated by 20 times using Amicon Ultra-15 Centrifugal Filter Units (Ultracel-10K, Merck).
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bioRxiv - Plant Biology 2022Quote: ... Wash fractions containing AgLTP24 purified were applied to a 3K MWCO Amicon Ultra-15 centrifugal filter (Merck Millipore) for buffer exchange with NH4+-free FBM medium (FBM-) ...
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bioRxiv - Microbiology 2022Quote: ... and subsequently concentrated to 250µl using Ultra Centrifugal Filters of 100kDa (Amicon Ultra-15, Merck Millipore, ref.:UFC9100) by repeated centrifugation at 4000xg for 15-60 minutes at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... The eluent was concentrated using an Amicon Ultra-15 100-kDa cut-off centrifugal filter unit (Merck Millipore) before purification on a Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: E10.5 embryos were fixed in 4% PFA/PBS for 1.5 h followed by incubation in 15% sucrose (Merck)/PBS for 2 h and overnight incubation in 30% sucrose/PBS ...
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bioRxiv - Microbiology 2022Quote: ... The eluant was concentrated and buffer was replaced with 10 mM Tris-HCl buffer (pH 8.0) in Amicon Ultra-15 (nominal MW limit, 10,000) (Merck). At each stage ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was concentrated to ∼500 μL using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore MWCO 3 kDa). The concentration of proteins was determined using JASCO V730 UV-vis spectrophotometer at 280 nm ...
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bioRxiv - Bioengineering 2022Quote: ... followed by 40-fold dilution using PBS and ultrafiltration using Amicon Ultra-15-30K centrifugal units (Merck Millipore) for buffer replacement.
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bioRxiv - Cell Biology 2023Quote: ... the purified protein was dialyzed against 20 mM HEPES using an Amicon ultra-15 (Merck, Rahway, NJ, USA).
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bioRxiv - Biophysics 2022Quote: ... The eluent was concentrated using an Amicon Ultra-15 100-kDa cut-off centrifugal filter unit (Merck Millipore) before purification on a Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... pH 8) and concentrated to 30 mg/ml using an Amicon Ultra-15 Centricon (Millipore Merck, Darmstadt, Germany). Crystallization experiments were performed with ORYX8 pipetting robot (Douglas Instruments ...
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bioRxiv - Pathology 2023Quote: ... iodioxanol was removed and the batches concentrated by passage through Amicon Ultra-15 tubes (Ultracel-100K; Merck Millipore). AAV8 vector without sgRNA was also produced as nonediting control ...
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bioRxiv - Molecular Biology 2023Quote: ... This protein sample was concentrated 10 times using a 3.5 kDa Amicon Ultra 15 mL device (#UFC500324, Merck) at 3,000 x g 80 minutes ...
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bioRxiv - Microbiology 2023Quote: ... centrifuged 5 min at 2000rpm and concentrated 10 times using Amicon® Ultra 15 mL Filters (Merck #UFC910096). The concentrated virus aliquoted and stored at -80°C.
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bioRxiv - Cell Biology 2023Quote: ... Vector particles were purified on an iodixanol gradient and concentrated on Amicon Ultra-15 100K columns (Merck-Millipore). The AAV vectors were titrated as vg per milliliter by quantitative real-time PCR using ITR2 (inverted terminal repeats ...
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bioRxiv - Immunology 2023Quote: ... Samples were then loaded on Amicon Ultra-15 centrifugal filters (10 kDa molecular weight cutoff, Merck Millipore # UFC9010) for imidazole removal and washed with purification buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Collected peak fractions were concentrated to 10-40 µM using −50kDa centrifugal filter tube (Amicon Ultra-15, Merck). Protein concentration was measured with a NanoDrop ND-1000 spectrophotometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated to 10 mL using an Amicon Ultra-15 10K MWCO Centrifugal Filter Unit (Merck Millipore) and loaded into SnakeSkin Dialysis Tubing ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing the target protein were concentrated to 5 mL with Amicon Ultra-15 10 kDa (Merck). The concentrated sample was loaded onto a 320 mL of HiLoad Superdex 75 (GE Healthcare Life Science ...
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bioRxiv - Plant Biology 2020Quote: ... and the buffer changed to 6 M urea using Amicon ultra-15 centrifugal filter units with 10,000 MWCO (Merck). The protein concentration was determined spectroscopically at 280 nm on a NanoDrop™ spectrophotometer based on the Lambert-Beer’s law assuming a molecular weight of the CBC-Qprot of 47921 Da and an extinction coefficient of 37,400 M−1 cm−1 ...
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bioRxiv - Developmental Biology 2020Quote: Precise numbers of oocytes per comparable experimental condition (~15-30) were washed in phosphate buffer saline (PBS; Merck, P5493) containing polyvinyl alcohol (PVA ...
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bioRxiv - Molecular Biology 2020Quote: ... cell culture supernatant of HUVECs was concentrated to 1 mL using Amicon-Ultra 15 Centrifugal Filter Units (UFC901096, Merck) and incubated with 0.5 µg of Alexa 488 labeled anti-CD9 ...
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bioRxiv - Biophysics 2022Quote: ... The filtered supernatant was transferred into Amicon Ultra-15 centrifugal filters (Ultracel-PL PLHK, 100kDa cutoff, Merck Millipore, UFC9100) and centrifuged at 4,000 g for 40 minutes at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Lentiviral supernatants were collected after 48 hours of incubation and concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck). Early passage mouse ESCs were transduced with the lentiviral particles in mouse ESC media supplemented with polybrene (8 µg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Filtered samples were centrifuged at 5000G using an Amicon ® Ultra 15 (100 kDa) (Merck Millipore, Tullagreen, Cork, IRL) following the manufacturer’s protocol (30 min of centrifugation for every 12 ml) ...
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bioRxiv - Biochemistry 2020Quote: ... Selected FdR9-containing fractions were concentrated by ultrafiltration using a 30 kDa cut-off membrane (Amicon ultra-15, Merck) and further purified by gel filtration on a Superdex 75 26/60 column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... fractions containing the complex DrmAB were pooled together and concentrated using 30 KDa NMWL Ultra-15 Amicon® (Merck). Protein concentration was estimated by the Bradford Assay (Thermo Scientific ...
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bioRxiv - Biophysics 2021Quote: ... The culture supernatant was collected after 72 h and concentrated using Amicon Ultra-15 MWCO 100 kDa (Merck Millipore).
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bioRxiv - Cancer Biology 2021Quote: Cells grown on coverslips were washed with PBS and incubated 15 min in 4% formaldehyde (formalin solution buffered at pH 6.8, Merck). Cells were washed once with 50 mM ammonium chloride (Carl Roth ...
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bioRxiv - Cell Biology 2021Quote: ... passed through a 0.45 um filter and concentrated using an Amicon Ultra-15 centrifugal filter unit (UFC910024, Merck/Millipore), Ultracel-100 regenerated cellulose membrane.
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bioRxiv - Cell Biology 2021Quote: ... 10 mL of the supernatant were concentrated using an Amicon Ultra-15 100 kDa filter (regenerated cellulose; Merck/Millipore) by centrifuging at 4.000 × g for 10 minutes ...
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bioRxiv - Biophysics 2021Quote: ... The fractions containing the capsid protein were pooled and concentrated using an Amicon Ultra-15 centrifugal filter (Merck Millipore), and the buffer was exchanged to a final storage buffer composed of 50 mM NaH2PO4/Na2HPO4 ...
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bioRxiv - Biochemistry 2022Quote: The culture supernatant containing proMbxA was concentrated with Amicon Ultra-15 Centrifugal Filter Units (50 kDa NMWL, Merck Millipore) to 1/10 of the starting volume and loaded on a 5 ml Ni2+ HiTrap IMAC HP column (GE Healthcare) ...
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Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin IIbioRxiv - Biophysics 2022Quote: ... This was followed by concentration with a centrifugal concentrator (Amicon Ultra-15 3 k device, Merck Millipore, Burlington, MA), and after supplementing with 10 mM DTT ...
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bioRxiv - Biophysics 2022Quote: ... The sample was concentrated to 100 μM using an Amicon Ultra 15 mL centrifugal filter (Merck KGaA, Darmstadt, Germany).
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bioRxiv - Cell Biology 2022Quote: ... Viral supernatants were collected after 36 h and 50 h and concentrated with Amicon ultra-15 centrifugation tubes (Merck). For storage and further usage samples were snap frozen in liquid nitrogen.
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bioRxiv - Microbiology 2020Quote: ... 500 μl of resolubilised stool was concentrated using Amicon® Ultra-15 3k centrifugal filters (Merck, Saint-Romain, France) for further studies (Figure 1) ...
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bioRxiv - Cancer Biology 2021Quote: ... and then concentrated using 15 ml Amicon spin filter cartridges (cutoff: 3 kDa, 10705884-Merck Millipore, Burlington, MA, USA) by serial addition of 10 ml of CM and centrifugation at 4000 x g until 1.5 ml were left ...
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bioRxiv - Cancer Biology 2022Quote: ... the protein extracts (15 μg/sample) were blotted on a primed 0.45 μm polyvinylidene fluoride (PVDF) membrane (Merck-Millipore). Protein spots were stained using Direct Blue (Sigma-Aldrich ...
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bioRxiv - Pathology 2022Quote: ... Nose and throat swabs were collected in tubes containing viral transport medium (15% sucrose, 2.5 μg/ml Amphotericin B [Merck] ...
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bioRxiv - Biophysics 2019Quote: ... 0.1 mM EDTA] with 0.2 mM ADP-Mg by ultrafiltration using AmiconUltra-15 10k and AmiconUltra-0.5 30k filters (Merck Millipore).
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing the proteins of interest were pooled and concentrated using Amicon Ultra-15 30K spin concentrators (Merck Millipore) before further purification using a HiLoad 16/600 Superdex 200 prep grade size exclusion column (GE Healthcare ...
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bioRxiv - Genomics 2021Quote: ... 2005) and the PGK viruses were concentrated approximately 40-fold using Amicon Ultra-15 centrifugal filter units (Merck #UFC910024), the HSP expressed viruses were used unconcentrated.
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bioRxiv - Immunology 2020Quote: ... and the fractions with the target protein were mixed and concentrated using Amicon Ultra-15 Centrifugal filters (Merck Millipore) with a 3 kDa membrane cutoff (Figure 10).
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bioRxiv - Neuroscience 2022Quote: Fixed tissue was cut into 1 cm fragments and first impregnated during 3h in a 15%-sucrose solution (Merck) in 1X PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Peak fractions containing SETDB1 Triple Tudor protein were pooled and concentrated to 2 mL in Amicon Ultra-15 concentrators 10,000 molecular weight cut-off (Merck Millipore ...