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Citations for Merck :
3751 - 3800 of 6398 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Titers were determined by transduction of Jurkat cells (ACC 282; DSMZ) in presence of 5 µg/ml Polybrene Infection/Transfection Reagent (Merck). After 48 h ...
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bioRxiv - Pathology 2023Quote: ... Then sections were blocked for 45 min using a blocking buffer containing 5% goat or donkey serum (depending on the host species of the secondary antibody) (#G9023 and #D9663, Merck) and 0.3M glycine (#56-40-6 ...
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bioRxiv - Neuroscience 2022Quote: ... Cubes of 1 cm3 were cut and incubated overnight in an aqueous solution of 150 mM NaCl (S9888, CAS 7647-14-5, Merck) and 10 µM fluorescein (46955 ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were subsequently used for hydrophilic interaction liquid chromatography-mass spectrometry analysis (Dionex UltiMate 3,000 RSLC, Thermo Fisher, using a 150 × 4.6 mm, 5 µm ZIC-pHILIC column, Merck Sequant). The column was maintained at 40°C and samples were eluted with a linear gradient (20 mM ammonium carbonate in water and acetonitrile ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction was then quenched by washing the cells three times with ice-cold PBS buffer containing 5 mM Trolox (Sigma-Merck), 10 mM sodium ascorbate ...
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bioRxiv - Immunology 2023Quote: ... 1 × 105 cells were plated per well and were further cultured in the presence or absence of 5 µM oligomycin A (Merck) at 37 °C in a humidified chamber containing 5% CO2 and 1% O2 for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: ... HeLa cells were cultivated at 37°C under 5% CO2 in MEM with Earle’s salts supplemented with 10% FBS superior (Merck KGaA), 2 mM L-Alanyl-L-Glutamine (Biochrom) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Peak fractions were pooled and concentrated to 5 μM (0.7 mg/mL) final concentration using an Amicon Ultra-15 (Merck Millipore) for further analysis.
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bioRxiv - Molecular Biology 2023Quote: ... samples were centrifuged at 14,000 rpm at room temperature for 5 minutes or filtered in multiscreenHTS-HV 0.45 µm 96 well filter plates with PVDF membranes (Merck Millipore) to remove cell debris and nucleic acid aggregates ...
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bioRxiv - Microbiology 2023Quote: Samples separation with chromatography was performed on the SeQuant ZIC-HILIC column (5 μm, 200 Å, 150x2.1 mm, Merck, Germany) with a flow rate of 0.6 mL/min ...
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bioRxiv - Microbiology 2023Quote: ... column 5 µm 150 x 4.6 mm with a 20 x 2.1 mm ZIC-pHILIC guard column (both Merck Millipore, Australia) (25 °C) ...
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bioRxiv - Biochemistry 2023Quote: ... Briefly this involved separation of metabolites using a ZIC pHILIC column (150 mm × 4.6 mm, 5 μm column, Merck Sequant) before entering an Orbitrap Q-Exactive (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... A SeQuant® ZIC®-HILIC 5 μm particle size analytical column 150 x 4.6 mm (Merck KGaA, Darmstadt, Germany) and a Acquity UPLC BEH C18 column 1.7 μm particle sized analytical column 2.1 × 100 mm connected to an Acquity UPLC BEH C18 VanGuard Pre-column ...
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bioRxiv - Biophysics 2024Quote: Glass coverslips (Paul Marienfeld GmbH, 24 x 50 mm, 170 ± 5 μm) were overnight incubated in 100 mM Sulphuric acid (Merck). Afterwards the coverslips were rinsed consecutively with Milli-Q water ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM MgCl2 buffer at a final concentration of 5 mg/mL using three consecutive cycles of freezing in liquid nitrogen and thawing in an ultrasonic bath (Merck). The rehydrated lipid solutions were extruded 21 times through a 100 nm diameter pore size polycarbonate membrane (Avanti Polar Lipids Inc.) ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting cell pellet was freeze-thawed thrice and re-suspended to 10% (w/v) in a lysis buffer containing 5% (v/v) Polysorbate 80 and 20 U/mL benzonase (Merck). After 1h incubation at 37°C ...
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bioRxiv - Physiology 2022Quote: ... They were then permeabilized with 0.02% Triton X-100 in PBS for 15 min and blocked with 5% bovine serum albumin (Sigma-Aldrich, Merck) in PBS for 3 h ...
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bioRxiv - Microbiology 2022Quote: ... fixed with cold methanol for 5 min and permeabilized with PBS containing 0.1% Triton X-100 (Merck Life Science, T8787). For the staining of acidic organelles ...
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bioRxiv - Microbiology 2022Quote: ... cells were cultured in 25 cm2 flasks at 37°C and 5% CO2 using advanced Dulbeccos modified Eagle medium (DMEM; TFS) with the addition of 10% fetal bovine serum (Merck), 50 U/mL penicillin and 50 μg/mL streptomycin (TFS) ...
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bioRxiv - Developmental Biology 2023Quote: ... All the oocytes harvested from COCs were transferred to activation medium composed of KSOM medium supplemented with 5 mM strontium chloride (13909, Merck) and 2 mM EGTA (A0878 ...
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bioRxiv - Pathology 2023Quote: ... lobus accessorius) were collected and fixed in 10 % neutral-buffered formalin (Chemie Vetrieb GmbH & Co) or 5 % glutaraldehyde (Merck KGaA) for microscopic and ultrastructural evaluation respectively.
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bioRxiv - Evolutionary Biology 2024Quote: ... The interlocking ring included a central odorant delivery platform comprising a 10-mm-diameter cover glass and two 5-mm-diameter filter discs (WHA10016508; Merck) for the evaluation of VOCs (stl files are provided in Extended Data Fig ...
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bioRxiv - Microbiology 2024Quote: ... were deposited using a semi-automated sample application system LINOMAT 5 (CAMAG) and further separated on TLC (Silica Gel 60, Merck) by using CHCl3-CH3OH (90:10 ...
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bioRxiv - Microbiology 2024Quote: ... One-centimeter length of colon was homogenized (50 mg/mL) in ice-cold 50mM potassium phosphate buffer (pH 6) containing 5% hexadecyl trimethyl ammonium bromide (Merck) and hydrogen peroxide (0.0005%) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were harvested at 5000 rpm for 5 min and resuspended in SDC medium containing appropriate concentrations of ethanol (Merck) for the indicated time points ...
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bioRxiv - Microbiology 2024Quote: ... washed three times with PBS-T and stained overnight at 4°C in PBS-T/5% milk containing primary antibodies (anti-phosphorylated STAT1, Cell Signalling Technology and anti-alpha Tubulin, Merck) [1:1000] ...
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bioRxiv - Molecular Biology 2024Quote: ... Milli-Q water suitable for LC-MS analysis was freshly obtained from Millipore Milli-Q® Advantage A10 system (18,2 MΩ×cm, <5 ppb) equipped with a LC-Pak® Polisher filter unit (#LCPAK0001) purchased from Merck Millipore (Darmstadt ...
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bioRxiv - Biochemistry 2024Quote: ... for 5-10 min at room temperature to reveal bound cytokine and reaction was blocked by adding HCl 2N (Merck) solution (20 μL/well) ...
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Gliflozins, sucrose and flavonoids are allosteric activators of lecithin:cholesterol acyltransferasebioRxiv - Biochemistry 2024Quote: ... PAM (0.1 mg/mL in DMSO) or DMSO were incubated with LCAT (5 μg/mL in assay buffer + 60 μM albumin (Merck)) 1:100 v/v at 37°C gently shaken ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin immunoprecipitation was performed using 5-10 μg of primary mouse monoclonal ANTI-FLAG® M2 antibody (Merck, F1804-200UG) in 250 μL LB3 with 1% Triton X-100 overnight at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was centrifuged (3215 g for 5 min) and then filtered (Merck Millex™ Syringe Filter, PES, 0.22 μm). In the enrichment phase ...
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bioRxiv - Biophysics 2024Quote: ... HeLa cells were cultivated at 37 °C under 5% CO2 in MEM with Earle’s salts supplemented with 10% FBS superior (Merck KGaA), 2 mM L-Alanyl-L-Glutamine (Biochrom) ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were heated at 95 °C for 5 min before loading onto Bis-Tris gels and wet transfer to a PVDF membrane (Merck). The membranes were then blocked with 5% BSA in TBS-T for 1 h and incubated with the appropriate primary antibodies overnight at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The cultures were incubated for 5 days at 30 °C and 130 rpm (Multritron, INFORS) using AeraSeal films (Merck, #A9224) on top of the flask ...
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Enabling Epitope Analysis of Glycoproteins via HDX-MS Using Antigens with Uniformed N-linked GlycansbioRxiv - Biochemistry 2024Quote: ... After nurturing the cells for 4-5 days in the Expi293™ Expression Medium with kifunensine at a concentration of 5 - 20 μM,9 the supernatants were gathered and filtered by a 0.22-µm filter device (Merck Millipore). The TALON® Superflow Metal Affinity Resin and HisTALON™ Buffer Set (Takara Bio ...
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bioRxiv - Genetics 2024Quote: ... An equal loading of all samples was run in parallel on a separate gel and stained with 5% Coomassie brilliant blue (Merck) to assess total protein loading ...
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bioRxiv - Developmental Biology 2024Quote: ... The lung sample was lysed for 2 min in ACK (ammonium-chloride-potassium) lysis buffer to remove erythrocytes and washed with FACS buffer (PBS containing 5% fetal calf serum, Merck) before continuing with blocking and staining ...
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bioRxiv - Cell Biology 2024Quote: ... Fungal cells were perfused into the plate chambers via microfluidic bursts at a pressure of 8 PSI for 5 seconds using the ONIX2 software (v 5.0; Merck, UK). This was repeated until a satisfactory cell distribution was achieved ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections were blocked with 5% normal donkey serum for 1 h at room temperature and incubated with primary antibodies (rabbit anti-GFAP 1:200, Dako; goat anti-Iba1, 1:500 Wako; NeuroTrace™ 1:200, Thermo Fischer; mouse anti-NeuN Antibody 1:500, Merck, #MAB377 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-β-tubulin (1:1000; clone KMX-1; Merck Millipore, Massachusetts, USA), mouse anti-acetylated α Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated for 1 hour with mEM48 (1:500, Merck Millipore, MAB5374) antibody at room temperature in TBS containing 0.1% TritonX-100 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-acetylated α-tubulin (clone 6-11B-1, Merck; ExM, 1:250), mouse anti-acetylated α-tubulin (T7451 ...
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bioRxiv - Immunology 2023Quote: ... were either immersed in a 1:1 mixture of 30% hydrogen peroxide (Merck) and concentrated sulfuric acid (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... at 1:500 dilution and nuclear stain DAPI (1 μg/ml, Merck, D9542) at 4°C with gentle rocking ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-DNA Ligase 1 (Sigma Aldrich, Merck, clone 5H5, MABE1905; 1:500); mouse anti-PARP1 (R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-γ-H2AX (Merck, 05-636, 1 mg/ml, dilution 1:1000). For the secondary antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... Part 1 of the precursor solution contained 20 mg mL-1 fibrinogen (Merck) diluted in DMEM/F12 with HEPES ...
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bioRxiv - Developmental Biology 2023Quote: ... they were incubated in 100 μg/mL DNAse 1 (1 mg/mL, Merck) in a concentration of 1000 cells/μL for 15 mins at RT ...
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bioRxiv - Cell Biology 2023Quote: ... at 1:2000 and/or DAPI (at 1:1000, ready-made solution, Merck).
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bioRxiv - Neuroscience 2022Quote: ... and mouse monoclonal anti-NUCB2/nesfatin-1 antibody (1:50; Merck Millipore, MABS1164), and b ...