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Citations for Merck :
3651 - 3700 of 6398 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... 1% Penstrep and 1 ng/ml TGFβ (Cat nb. T7039, Merck, Sigma) or 20 ng/ml PDGF-AA (human PDGF-AA ...
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bioRxiv - Biophysics 2022Quote: ... cells were diluted in in HEPES buffered DMEM/F12 1:1 (Merck) supplemented with 10% fetal bovine serum and 2 mM L-glutamine to 50,000 cells/ml and plated at a density of 5000 cells per well (100 µl ...
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bioRxiv - Plant Biology 2020Quote: ... supplemented with 1 mM PMSF and 1 X cOmplete protease inhibitors (Merck) and lysed with a French press under 1200 PSI ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... and goat anti-Mouse IgG-HRP (1:1000-1:3000, Merck Millipore). The kinase reactions using recombinant McIdas were performed as follows ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... and goat anti-Mouse IgG-HRP (1:1000-1:3000, Merck Millipore).
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg/mL (1:500 dilution) of biotinylated anti-phosphotyrosine 4G10 (Merck) antibody and incubated for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... rabbit anti-PRRX16 (1:200) rabbit anti-Laminin (Merck, #L9393 1:200). Secondary antibodies used in these studies were procured from Thermo Fisher Scientific (Waltham).
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bioRxiv - Biochemistry 2024Quote: ... 1:1’000) and mouse anti-EF1a (Merck Millipore, dilution WB 1:10’000). Antibodies previously produced in our laboratory are ...
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bioRxiv - Biochemistry 2023Quote: ... A 1:1 mixture of magnetic hydrophobic and hydrophilic Seramag speedbeads (MERCK) were added at a 2:1 (w/w ...
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bioRxiv - Genetics 2024Quote: Cells were lysed in 1% SDS plus 1:200 Benzonase (Merck, #E1014) for 20 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... and 1 mL of 65 mg mL−1 sulfo-NHS (Merck 56485) were added to the reaction ...
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bioRxiv - Microbiology 2020Quote: ... Expanded and chopped gels were stored at 4°C in ddH2O immobilized prior to imaging on PDL-coated glass chambers (Merck, 734-2055).
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bioRxiv - Microbiology 2021Quote: Embedding in Lowicryl K4M (Chemische Werke Lowi, Waldkraiburg, Germany) was carried out on Vero cells fixed either in 4% formaldehyde (Merck, Darmstadt, Germany) or in 2% glutaraldehyde at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... spherical assembloids were collected in a PCR tube and immediately incubated in BBB working medium with 10 μM 4 kDa dextran-FITC (FD4; Merck, cat. #46944) or 10 μM fluorescently labeled human transferrin (Tf488 ...
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Farnesyltransferase inhibition overcomes the adaptive resistance to osimertinib in EGFR-mutant NSCLCbioRxiv - Cancer Biology 2022Quote: ... fixed with paraformaldehyde 4 % solution for 10 min and stained with a solution containing PBS - 0.5% crystal violet (Sigma-Aldrich/Merck, ref: C3886) – 25% methanol for 10 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... concentration and buffer exchange to TE with an Amicon Ultra-4 centrifuge filter unit (30 kDa cut-off, Merck Millipore, Cat#UFC803024).
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Immunology 2021Quote: ... These fractions were then pooled and buffer exchanged using SnakeSkin™ dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096). The clinically relevant mAbs tested here were in-house produced versions of ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein containing fractions were pooled and buffer exchanged using SnakeSkin™ dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096), as described previously (5).
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bioRxiv - Biochemistry 2022Quote: Collected samples from the first main peak were concentrated by ultrafiltration on 10K Amicon® Ultra-4 Centrifugal Filters Units (Merck Millipore) in a swinging-bucket rotor 4 000 g ...
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bioRxiv - Biochemistry 2022Quote: ... The TEV cleaved complex was concentrated to around 500 μl using a 4 ml Amicon® Ultra centrifugal filter concentrator (Merck Millipore) with a membrane molecular weight cut off 10 kDa ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were administered a lethal dose of sodium pentobarbital and transcardially perfused with Ringer’s solution followed by paraformaldehyde (PFA, 4 %, Merck Millipore, Darmstadt, Germany) in 125 mM phosphate buffer (PB) ...
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bioRxiv - Neuroscience 2022Quote: ... Sonicated sample was diluted with 3.5 ml PBS and concentrated to 500 μl by diafiltration using an Amicon® Ultra-4 Centrifugal Filter Unit with 30 KDa cutoff (Merck UFC803024) to remove traces of sarkosyl ...
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bioRxiv - Neuroscience 2022Quote: ... and concentrated to 300 ul by diafiltration using an Amicon® Ultra-4 Centrifugal Filter Unit with 30 kDa cutoff (Merck UFC803024) to remove the potentially unincorporated dye ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein containing fractions were pooled and buffer exchanged using SnakeSkin dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096), as described previously (44).
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bioRxiv - Microbiology 2024Quote: ... the same dilutions were also asdded to Eosin Methylene Blue Agar and MacConkey Agar both supplemented with cefotaxime (4 mg/L) (Merck, Darmstadt, Germany) for the Extended-spectrum beta-lactamase phenotype (37) ...
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bioRxiv - Plant Biology 2024Quote: The following chemicals were used in the DEEP-Clear (Pende et al., 2020) adapted version to plant tissues: PFA (paraformaldehyde) (CAS no. 30525-89-4, Merck, http://www.merck.com/), xylitol (CAS no ...
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bioRxiv - Microbiology 2024Quote: ... The flow-through was further concentrated from 10 ml volume in an Amicon® Ultra-4 Centrifugal Filter 50 kDa unit (Merck Millipore) and stored at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... and loaded in 4–12% NuPAGE Bis-Tris gel (Fisher, 10247002) for subsequent electrophoresis and transference to nitrocellulose membrane (Merck-Sigma, GE10600003). Precipitated RNA was analyzed by autoradiography and FLAG-PTBP protein levels were measured by western blot.
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was filtrated through a filter paper (Whatman No. 4, Whatman plc, Kent, UK) and a membrane filter (MF-milliporeTM 0.22 μm, Merck KGaA, Darmstadt, Germany). The solution was cleaned up through a Waters Sep-Pak C18 column (Waters ...
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bioRxiv - Biophysics 2023Quote: ... containing 0.3□mM biotin and concentrated at +4 °C to 25 μl volume using Amicon ®Ultra 10 kDa MWCO centrifugal filters (Merck Millipore).
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bioRxiv - Biochemistry 2023Quote: Total cell lysates of HEK293 cells stably expressing C-terminally Flag M2-tagged SIRT4 were incubated overnight at 4°C with anti-Flag M2 agarose beads (Merck, Darmstadt, Germany). The beads were washed three times with washing buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue homogenisation and western blotting was performed with 50 μg of total protein as previously described.15 Membranes were incubated overnight with gentle agitation at 4 °C with the MSH3 antibody (Merck MSH3MAB(1F6)) in 5% milk PSB-T (phosphate buffer saline (PBS) ...
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bioRxiv - Bioengineering 2023Quote: ... at 40,000 xg for 20 min at 4°C and the supernatant was filtered through a 0.45 µm Millex® syringe filter (Merck, Darmstadt, Germany). Clarified lysate was loaded onto a nickel (II ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 0.4–0.5 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche), and the cells were fixed 5–6 days later ...
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bioRxiv - Microbiology 2024Quote: ... the SN was concentrated by ultrafiltration at 4000□g and 4°C using 100 K Amicon® Ultra-15 centrifugal filters (Merck Millipore). The concentrated SN was filtered through a 0.45-µm-pore-size filter ...
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bioRxiv - Cell Biology 2024Quote: ... cellular morphology was documented in HL-60 and K-562 by staining with 4-Nitro blue tetrazolium chloride (NBT) (James et al., 1997) () or Neutral Red solution (Merck/Sigma-Aldrich) (Russo et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM FBP (Merck) (where applicable) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μM dexamethasone (Merck), 5 μg/mL insulin (Merck ...
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bioRxiv - Neuroscience 2022Quote: ... 1 NADPH (Merck-Millipore), and 0.2% Triton X-100 ...
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bioRxiv - Immunology 2021Quote: ... and 1 % methylcellulose (Merck). The PRRSV-2 16CB02 isolate (referred to as PRRSV-2 ...
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bioRxiv - Cell Biology 2021Quote: ... and 1% NH4OH (Merck) right before infusion into the MS at a flow rate of 10 μL/min ...
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bioRxiv - Physiology 2022Quote: ... or Mdivi-1 (Merck) at the times and concentrations indicated in the figure’s legends.
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bioRxiv - Cancer Biology 2022Quote: ... 1 µM staurosporine (Merck), vehicle control or compounds from the small molecule library (Supplementary Table 4 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 mM DTT (MERCK, Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Wiskostatin (Merck, 1 μM), CCL19 (Peprotech ...
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bioRxiv - Bioengineering 2022Quote: ... 1× ITS (Merck, I3146), 500 µM monothioglycerol (Sigma–Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 7.4 (Carl Roth),125 mM KCl (Merck),1 mM MgCl2 (Merck),1 mM EGTA/KOH pH 8.0 (Carl Roth),5% glycerol (Merck),1% NP-40 (Nonidet P 40 Substitute ...
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bioRxiv - Pathology 2021Quote: ... 1 mM EDTA (Merck), 1% Triton X-100 ...
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bioRxiv - Cell Biology 2021Quote: ... 1% sucrose (Merck # 84100) and 2.5□mM morpholinoethanesulfonic acid (Euromedex # EU0033 ...