Labshake search
Citations for Merck :
3551 - 3600 of 6398 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2020Quote: ... of total protein suspended in loading buffer were separated by SDS-PAGE using 8% or 4-20% gradient gels and transferred onto PVDF membranes (Immobilon-P or Immobilon PSQ, Merck Millipore) for immunostaining ...
-
bioRxiv - Microbiology 2023Quote: ... Fractions containing the protein of interest were pooled and concentrated using an Amicon® Ultra-4 3K centrifugal filter unit (Merck) according to the manufacturer’s recommendation ...
-
bioRxiv - Immunology 2023Quote: ... the supernatant was concentrated by centrifugation at 1000 g at 4 °C through Amicon® Ultra 15 mL Centrifugal Filters (100 K; Merck). Finally ...
-
bioRxiv - Neuroscience 2023Quote: ... quality of the elution was assessed by loading 50µl of the resulting elution on a pre-cast 4-15% gradient gel (Merk, MP41G15) and incubated with SYPRO RUBY (Merck, S4942) protein gel stain ON at RT ...
-
bioRxiv - Biophysics 2023Quote: ... and fractions containing histone octamers were pooled and concentrated in an Amicon Ultra-4 Ultracell 30kDa centrifugal filter (Merck-Millipore #UFC803024). The protein concentration was determined with Bio-Rad Protein Assay Dye Reagent (Bio-Rad # 5000006).
-
bioRxiv - Molecular Biology 2022Quote: ... Peak fractions containing the Swi2-Swi5 complex were concentrated using an Amicon Ultra-4 (50 k) centrifugal filter (UFC805008, Merck Millipore). After cleavage ...
-
bioRxiv - Synthetic Biology 2022Quote: ... This solution was incubated at 37°C for 3-4 h before being transferred to a 250 mL flask containing 20 mL of 425-600 μm glass beads (Merck G9268) and 30 mL of sterile MilliQ water ...
-
bioRxiv - Plant Biology 2022Quote: ... Fractions containing CLPB3 were pooled and concentrated in Amicon® Ultra-4 Centrifugal Filter Units (Ultracel®-3K, Merck Millipore Ltd), with a subsequent buffer exchange to 6 M Urea ...
-
bioRxiv - Cancer Biology 2022Quote: ... Samples were resuspended in 80% methanol-water and a volume of 4 μL was injected on a SeQuant ZIC/pHILIC Polymeric column (Merck Millipore). The separation of metabolites was achieved at 25°C with a flow rate of 0.20 ml/min ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tumors were isolated from lungs and a tissue reference piece was separated and fixed in 4% paraformaldehyde (MERCK, Kenilwork, NJ, USA) overnight at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... The samples were next supplemented with 100 µl of 4 mM methoxypolyethylene glycol maleimide (PEG-maleimide) (5000; Merck, cat. No. 63187) in the SDS buffer ...
-
Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... were electrophoresed on SDS polyacrylamide gels ( NuPAGE™ 4-12% Bis-Tris Protein Gels; InvitrogenTM) and transferred to PVDF membranes (Millipore; Merck). Membranes were blocked in 2.5% (w/v ...
-
bioRxiv - Neuroscience 2024Quote: ... IL-10 and TNF-α were quantified in the FN of NL and at 21dpi aged GFAP-IL6Tg and WT mice (n= 4-6/experimental group) using a Milliplex MAP Mouse High Sensitivity kit (#MHSTCMAG-70K; Merck Millipore), following the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... Seeds were surface-sterilized by 70% ethanol and washed 4 times with sterilized ultra-pure water (Milli-Q water, Merck-Millipore) followed by stratification at 4 ºC for 2 d ...
-
bioRxiv - Neuroscience 2023Quote: ... Separated proteins were wet-blotted (110 V; 90 min; 4°C) on fluorescence-optimized polyvinylidene difluoride (PVDF) membranes (Immobilon-FL; Merck; IPFL00010) in transfer buffer (25 mM Tris (pH 7.6) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the supernatants were transferred to new tubes and incubated overnight at 4 °C with 15 µl of Anti-FLAG® M2 Magnetic Beads (Merck) with gentle mixing ...
-
bioRxiv - Bioengineering 2023Quote: ... filtered through 0.45 µm syringe filters (for Cas9/sgRNA delivery experiments) and concentrated using 100kDa MWCO Amicon Ultra-4 Centrifugal Filter Units (Merck Millipore) to volumes of 100-250 µL.
-
bioRxiv - Neuroscience 2023Quote: ... rats were anesthetized and underwent transcardial perfusion with 100 mL of saline followed by 100 mL of 4% paraformaldehyde (Merck, Germany) at 72 h following ICH induction ...
-
bioRxiv - Microbiology 2023Quote: ... 10% (v/v) glycerol using an Amicon Ultra-4 centrifugal column with a 10 kDa molecular weight cut-off (Merck-Millipore). This process yielded a protein concentrated to ∼10–20 mg/mL ...
-
bioRxiv - Cell Biology 2022Quote: ... the samples were first concentrated by centrifugation at 4,000 x g at 21°C using Amicon® Ultra-4 Centrifugal Filter Unit with 100kDa molecular weight cutoff (UFC810024, Merck Millipore ...
-
bioRxiv - Plant Biology 2022Quote: ... Fractions containing BirA were pooled and concentrated in Amicon® Ultra-4 Centrifugal Filter Units (Ultracel®-3K, Merck Millipore Ltd), with a subsequent buffer exchange to 6 M Urea ...
-
bioRxiv - Biophysics 2024Quote: ... The isolated protein was concentrated using Amicon® Ultra centrifugal filter with a molecular cutoff 10 kDa (Amicon Ultra-4 Centrifugal filter unit 10K, Merck). The SEC peak fraction for recombinant α-HL was further loaded into 10% SDS-PAGE (Coomassie Brilliant blue R 250 ...
-
bioRxiv - Biochemistry 2024Quote: ... and the fractions with the highest concentrations of mutant PGA proteins were concentrated into PBS using Amicon Ultra-4 Centrifugal Filters with a 10 kDa molecular weight cutoff (Merck Millipore). This process resulted in a protein with a purity exceeding 70 % and a concentration of approximately 1 mg/mL ...
-
bioRxiv - Molecular Biology 2024Quote: ... Remaining chromatin extracts were incubated overnight at 4°C with 50 µL of prewashed anti-Flag M2 magnetic beads suspension (Millipore, Merck M8823) per sample ...
-
bioRxiv - Plant Biology 2024Quote: ... 6xHis:SidK:3xFLAG or 6xHis:SidKF62A:3xFLAG protein were added to 1 ml of filtered extract to a final concentration of ∼2.5 μM and incubated in a rotatory mixer at 4°C with 25 μl of Anti-FLAG® M2 Magnetic Beads (Merck). 30 μl of the mixture was taken as input for Western blot analysis before adding magnetic beads ...
-
bioRxiv - Immunology 2024Quote: ... the slices were cultured for 4-6 days onto culture membranes (uncoated 30 mm Millicell-CM tissue culture inserts with 0.4 mm pores, Merck Millipore, Germany) and kept in six-well dishes (BD Biosciences ...
-
bioRxiv - Neuroscience 2024Quote: ... followed by overnight incubation at 4°C with primary antibodies (synaptophysin Cat#MAB329, Merck Millipore; GluA2 Cat#13607, Cell Signaling; Psd95 Cat#MAB1598, Merck Millipore ...
-
bioRxiv - Microbiology 2024Quote: ... Pan-Biotech) while the other retina was incubated for 4 h in the same medium supplemented with 100 µM leupeptin (Merck, L2884) and 20 mM NH4Cl (Merck).
-
bioRxiv - Immunology 2024Quote: ... ankle tissues were soaked in 4% (v/v) paraformaldehyde phosphate buffer solution (Fujifilm Wako) overnight and then replaced with OSTEOSOFT (Merck Millipore) every three days over 11 days for decalcification ...
-
bioRxiv - Plant Biology 2024Quote: ... and the supernatant was filtered through a hydrophilic polytetrafluoroethylene membrane filter (Millex-LG, 0.2 um × 4 mm, Merck Millipore, Darmstadt, Germany) and transferred to a screw-top glass vial ...
-
bioRxiv - Neuroscience 2024Quote: ... and equal amounts of proteins were separated by SDS-PAGE (10% or 4-20% gradient gels) and transferred to Immobilon-P membranes (Merck-Millipore). After chemiluminescence detection ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were blocked in 10% normal goat serum in TBS-T for one hour at room temperature and then incubated in the following primary antibodies suspended in blocking buffer overnight at 4°C: pS129 (El-Agnaf laboratory, 1µg/ml 27) and 5G4 (MABN389, Merck, 2µg/ml) combined with either MGO-aSyn (1µg/ml ...
-
bioRxiv - Immunology 2021Quote: ... The plates were then washed 5 times with HSWB followed by incubation of goat anti-mouse antibody (Merck, Germany) conjugated with HRP (diluted 1:3000 in LSWB + 1% BSA ...
-
bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
-
bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
-
bioRxiv - Neuroscience 2020Quote: ... also in with PBS containing 2.5%, bovine serum albumin (BSA; A1391-0250; Applichem, Germany) and 0.1 % Tween20 (9005-64-5; Merck, Germany). For visualizing neuronal membranes ...
-
bioRxiv - Neuroscience 2020Quote: ... and were then blocked with PBS containing 2.5%, bovine serum albumin (BSA) (A1391-0250; Applichem, Germany) and 0.1 % Tween20 (9005-64-5; Merck, Germany) for 1 hour ...
-
bioRxiv - Neuroscience 2020Quote: ... neurons were permeabilized and blocked with PBS containing 2.5%, bovine serum albumin (BSA) (A1391-0250; Applichem, Germany) and 0.1 % Tween20 (9005-64-5; Merck, Germany) for 1 hour ...
-
bioRxiv - Cancer Biology 2020Quote: ... Tumors and lungs were paraffin-embedded and consecutive 5-μm sections were stained with hematoxylin-eosin purchased from Merck.
-
miR-206 inhibits estrogen-induced proliferation and invasion of ER-α36 positive gastric cancer cellsbioRxiv - Cancer Biology 2021Quote: ... The PVDF membrane was sealed with 5% skim milk powder in the PBS containing tween (Sigma-Aldrich; Merck KGaA) for 3 h ...
-
bioRxiv - Cell Biology 2021Quote: ... The supernatant was clarified by centrifugation (500g, 5 min) and filtration (0.45 μm, Millex-HV, Merck Millipore (Burlington, MA)) ...
-
bioRxiv - Biochemistry 2022Quote: ... diluted 1:1 with dilution buffer (50 mM Hepes pH7.5, 150 mM NaCl, 5 mM MgCl2, 0.625 U/ul Benzonase (Merck, 70746)) ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were lysed in lysis buffer (30 mM HEPES pH 7.5, 400 mM NaCl, 20 mM imidazole, 5% glycerol, 125 U/ml benzonase (Merck), 1 mM MgCl2 and complete protease inhibitor cocktail [Roche] ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were lysed in lysis buffer (50 mM HEPES pH 7.5, 150 mM NaCl, 5% glycerol, 125 U/ml benzonase (Merck), 1 mM MgCl2 and complete protease inhibitor cocktail [Roche] ...
-
bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
-
bioRxiv - Systems Biology 2020Quote: The samples were analyzed using a high-resolution mass spectrometer in Orbitrap Q Exactive Plus (Thermo) equipped with a SeQuant ZIC-pHILIC column (150 mm x 2.1 mm x 5-micron packing, Merck) and a ZIC-pHILIC guard column (20 mm x 2.1 mm x 5-micron packing ...
-
bioRxiv - Microbiology 2020Quote: ... The HPLC column RP18e Purospher-STAR (Hibar) (250 × 4.6 mm; 5 μm) was used for chemical fingerprinting (E. Merck) and Eclipse 5 μm column (9.4 × 250 mm ...
-
bioRxiv - Molecular Biology 2022Quote: ... CAS-No: 7647-14-5) and ultrapure water from a Milli-Q system were supplied from Merck (Darmstadt, Germany).
-
bioRxiv - Systems Biology 2022Quote: ... of dimensions 150 mm x 2.1 mm x 5-micron packing and in sequence with a ZIC-pHILIC guard column (Merck) of dimensions 20 mm x 2.1 mm x 5-micron packing with the LC-MS/MS settings and calibrations as described previously (Iyer et al. ...
-
bioRxiv - Genetics 2022Quote: ... The tissue sections were then dried for 5 min at 37°C and mounted with 85% glycerol (Merck, 104094) and a coverslip ...