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Citations for Merck :
4001 - 4050 of 6398 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... BioTracker NIR680 (1:2000, Merck), and goat anti-rabbit Alexa Fluor 488 secondary antibody (1:500 ...
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bioRxiv - Biophysics 2024Quote: ... 1 % yeast extract (Merck, 1.03753.0500), and 0.02 % adenine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... α-Synapsin (1:500, Merck AB1543), α-Synaptophysin (1:250 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% Penicillin/Streptomycin (Merck, P0781) and 1% Amphotericin B (Merck ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 µg/mL Hydrocortisone (Merck), 10 mM Glutamine (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 µg/mL Hydrocortisone (Merck), 10 mM Glutamine (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... α-Chat (1:100, Merck AB144P), α-beta-Actin (1:4.000 ...
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bioRxiv - Cell Biology 2024Quote: ... α-Synapsin (1:500, Merck AB1543), α-myosin heavy chain (MyHC ...
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bioRxiv - Genomics 2021Quote: ... The lysate was incubated for 2 hours at 4°C with 180 µl of anti-flag agarose beads (50 % slurry, ANTI-FLAG® M2 Affinity Gel, Merck, Kenilworth, NJ, USA), prewashed 3 × with 1 ml isotonic wash buffer (IsoWB ...
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bioRxiv - Neuroscience 2023Quote: ... 10-15 neurospheres and 4-6 organoids per cell line were rinsed twice with PBS and then lysed in RIPA buffer (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The crude radiolabeling mixture was purified through successive cycles of ultracentrifugation using Amicon Ultra-4 centrifugal filter units (Merck, Darmstadt, Germany, MWCO 30 kDa) with the addition of formulation buffer (5 mg/mL ascorbic acid in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... rat polyclonal anti-Vangl2 (clone 2G4, 1:200 for Western blotting, 1:400 for immunoprecipitation, 1:500 for immunostaining, Merck Millipore, catalog no. MABN750), mouse monoclonal IgG1k anti-GFP (clones 7.1 and 13.1 ...
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bioRxiv - Cell Biology 2020Quote: U-2-OS Tet-On cell lines expressing eGFP-TopBP1 WT were grown under standard conditions (37°C, 5% CO2) in modified McCoy’s 5a medium (Merck-Sigma-Aldrich, M9309) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2021Quote: ... Pellets were thawn on ice and resuspended in lysis buffer (5 mL/g pellet: 50 mM Na2HPO4/KH2PO4, 150 mM NaCl, 0.2 % Tergitol™ solution (Merck, Darmstadt, Germany), and 2 mM DTT ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μl of sample was injected in 50 % B and the analysis was performed using the gradient of 15 % A and 85 % B for 3.5 min followed by an increase to 75 % A and 25 % B over 11.5 min and its continuation for 10 min with the SeQuant® ZIC®-pHILIC column (5 µm, 150 mm x 2.1 mm, Merck, USA), at a flow rate of 0.13 ml/min.
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bioRxiv - Microbiology 2020Quote: ... water was purified by a Milli-Q system (resistivity 18.2 MΩ cm at 25°C, TOC < 5 ppb, Merck Millipore, Molsheim, France). Indole-3-acetic-2,2-d2 acid (≥ 98% ...
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bioRxiv - Neuroscience 2020Quote: ... half of the medium was replaced with fresh medium containing 20 μM of 5-fluorodeoxyuridine and uridine (Sigma Aldrich, Merck, Darmstadt, Germany) every 3 days ...
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bioRxiv - Molecular Biology 2021Quote: ... mESCs were grown on gelatin-coated plates under standard culture conditions (37°C, 5% CO2, humid) atop a ‘feeder’ layer of MitomycinC-inactivated (Merck Life Science) SNLP mouse fibroblasts and passaged upon ∼80% confluency every 2-3 days using TrypLE Express (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... 30 mL buffy coat was diluted with 5 mL DPBS (BioWhittaker®) and carefully poured over 15 mL of Histopaque® (Merck). After centrifugation (500 g ...
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bioRxiv - Cell Biology 2023Quote: ... Protein transfer was then visualized using 0.1 % Ponceau S solution (w/v) in 5% acetic acid (Merck Life Sciences, Cat #: P7170-1L) and imaged ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A small aliquot of each sample was added to Hemi medium17 with a 5-μl/ml antibiotic cocktail (P4083, Merck, Darmstadt, Germany) and incubated under dark conditions at 19–20 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: CC strips were dissected and then transferred to microtiter plate wells containing 5 μM bis-N-methylacridinium nitrate (Lucigenin; Merck, Darmstadt, Germany), some strips were stimulated with NADPH (100 μM ...
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bioRxiv - Bioengineering 2023Quote: ... two thirds of the medium was removed from all wells and T cells were treated with 5 ng/mL polybrene (Merck, Darmstadt, Germany). Lentiviral particles (MOI=3 ...
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bioRxiv - Neuroscience 2024Quote: ... Postoperative care included antibiotic and analgesic administration (2.27% enrofloxacin, 5 mg/kg, Bayer, Kiel, Germany and 0.3 mg/ml buprenorphine, 8 µg/kg, Buprex, Merck & Co., Inc., NJ, USA) in a controlled temperature environment (37°C) ...
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bioRxiv - Microbiology 2024Quote: ... one stationary liquid culture was poured into a 50 mL syringe and plunged through a sterile 5 μm membrane filter (Millex-SV, Merck, Darmstadt, Germany), with slow and consistent pressure ...
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bioRxiv - Plant Biology 2024Quote: ... cinerea spores from glycerol stocks were diluted to a concentration of 5 x 104 spores/ml in ¼ PDB (Merck KGaA, Darmstadt, Germany) and pre-germinated for 4 hours at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Water was purified by a Milli-Q system (resistivity 18.2 MΩ cm at 25°C, TOC < 5 ppb, Merck Millipore, Molsheim, France). For laboratory culture samples ...
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bioRxiv - Cell Biology 2022Quote: ... protease inhibitor cocktail [5 μl/ml] and phosphatase inhibitor cocktail [10 μl/ml]) and 0.5% Nonidet P-40) (I8896; Sigma-Aldrich/Merck group, USA). After 10 min on ice ...
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bioRxiv - Cell Biology 2022Quote: ... Cell supernatant (50 mL) was then concentrated to 5 mL by centrifugation using Amicon® Ultra-15 Ultrafiltration Centrifuge Tubes (Millipore; Merck KGaA) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... were left to soak for no less than 24 hours in either plain water (for the cryptic/plain treatment) or a 2.5% Bitrex™ aqueous solution (5 g denatonium benzoate (Merck, Darmstadt, Germany) dissolved into 200 ml water ...
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bioRxiv - Microbiology 2022Quote: ... solution containing 16 mM NaHCO3 and 0.15 mM ZnSO4 as well as on 5 mg/ml imipenem – 5 mg/ml cilastatin in 0.15 mM ZnSO4 prepared from the brand name Primaxin formulation (Merck, Sharp& Dohme Corp.) were also examined.
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bioRxiv - Microbiology 2022Quote: ... 0.5 ml of each aliquoted sample was filtered onto a 25 mm polycarbonate filter (pore size: 0.45 µm, Merck KGaA, Darmstadt, Germany). The filters were then air dried under sterile conditions and mounted onto 25mm diameter aluminum pin stubs (Agar Scientific Ltd. ...
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bioRxiv - Microbiology 2022Quote: ... the medium was replaced with complete medium without phenol red and 10 μL of 5 mg/mL MTT solution (In vitro toxicology assay kit, MTT-based, TOX-1KT, Merck, Darmstadt, Germany) were added to each well ...
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bioRxiv - Microbiology 2022Quote: ... Frozen larvae were thawed for two min in a solution of 50:50 5% bleach (Danklorix, CP GABA, Hamburg, Germany) and Milli-Q (Merck, Darmstadt, Germany) water for surface sterilization (Amiri et al. ...
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bioRxiv - Cancer Biology 2023Quote: A Thermo Ultimate 3000 high performance liquid chromatography (HPLC) system was equipped with a ZIC-pHILIC column (SeQuant; 150 mm by 2.1 mm, 5 μm; Merck KGaA, Darmstadt, Germany), with a ZIC-pHILIC guard column (SeQuant ...
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bioRxiv - Cell Biology 2023Quote: ... fixed cells were pelleted (1500 RPM 5 min) and resuspended in PBS containing 20 µg/ml propium iodide and 200 µg/ml RNase (Merck Life science).
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bioRxiv - Molecular Biology 2024Quote: ... The tissue was washed in PBS and incubated in 5 mM EDTA solution in PBS (pH 8; Merck Millipore, Burlington, MA, USA) at 4°C for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... the homogenized samples were incubated for 5 min at RT and 100 µL of liquid chloroform (Merck Millipore, Fontenay Sous Bois, France) were added ...
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bioRxiv - Cell Biology 2024Quote: ... The peak fractions were concentrated to approximately 5 mg/ml using an Amicon Ultra filter (Merck, molecular weight cut-off 10 kDa). The sample was subsequently supplemented with glycerol at a concentration of 10 %v/v ...
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bioRxiv - Developmental Biology 2024Quote: ... The females were stimulated 46 h prior to oocyte isolation using 5 IU of pregnant mare serum gonadotropin (PMSG; Folligon; Merck Animal Health) per mouse ...
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bioRxiv - Bioengineering 2021Quote: ... and stained with 1 mL of 1 mg/mL of Direct red solution (Merck KGaA) in saturated aqueous solution of picric acid (Merck KGaA ...
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bioRxiv - Neuroscience 2020Quote: ... guinea pig anti-vGluT1 and -vGluT2 (1:2000; Chemicon) and anti-vGluT3 (1:2000; Merck), mouse anti-Lhx6 (1:X ...
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal pS129 (11A5, 1:10,000) and tyrosine hydroxylase (TH, #AB152, Merck Millipore, 1:1000). Appropriate secondary Alexa Flour antibodies (Life Technologies and Abcam ...
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bioRxiv - Microbiology 2024Quote: ... anti-CycB (sc-245, Santa-Cruz, 1:300) or anti-tubulin (T5168, Merck, 1:40000) and the following secondary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... with protease inhibitor cocktail (1:1,000) and phosphatase inhibitor cocktail (1:400; Sigma-Aldrich/Merck) by sonication (VCX 130 PB ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then diluted 1:1 (v:v) with water containing 4mM MgCl2 and Benzonase (Merck #70746 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... incubated 1 min in cresyl violet dye (1% of powder in absolute ethanol, Merck Millipore), then held 5 sec in 90% ethanol ...
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bioRxiv - Neuroscience 2024Quote: ... anti rabbit A32732 (AF488) 1:750), as well as DAPI (D9542-1 mg, MilliporeSigma (Merck) 285 ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by primary antibody incubation (SOX2, 1:500, Bio-techne, AF2018; SOX9, 1:500, Merck, AB5535 ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated at 4°C overnight with primary antibodies (HIF1α, 1:1000, Novus Biologicals, NB100-134; HIF2α, 1:1000, Cell Signaling Technology, 7096; β-Actin, 1:5000, Merck, A1978) in 0.1% skimmed milk in TBST buffer (blocking buffer) ...