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Citations for Merck :
2701 - 2750 of 2776 citations for Neural Precursor Cell Expressed Developmentally Down Regulated 9 NEDD9 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated 48 hours at 37°C with BrdU (15 μM, stock solution 30 mM in H2O) (Merck, Darmstadt, Germany). After washing two times with warm PBS the cells were pre-extracted with ice cold NuEx buffer and fixed with 2% (w/v ...
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bioRxiv - Physiology 2024Quote: ... For colocalization 1:100 anti-CHRM3 was incubated with 1:500 anti-Clara Cell Secretory Protein (CCSP; Merck Millipore cat#07– 623), 1:200 anti-alpha tubulin (Santa Cruz ...
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bioRxiv - Cancer Biology 2024Quote: Medium conditioned by MDA-MB-468 cells was loaded into size exclusion columns (Amicon® Ultra-15 Ultracel-10 Centrifugal Filter Unit, Merck, #UFC901024) centrifuged at 4,000g for 30 min and the fractions stored at -20⁰C until further analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... colonies from MDA-MB-468 cells were fixed by replacing the medium with a solution of 3% trichloroacetic acid in water (Merck, #T6399-500G), rinsed twice with water ...
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bioRxiv - Immunology 2024Quote: ... U-937 cells (2×105 cells/mL) were incubated in the culture medium containing 200 ng/mL of phorbol myristate acetate (PMA) (Sigma-Aldrich; Merck KGaA) for 24 h to induce differentiation into macrophage cells ...
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bioRxiv - Genomics 2024Quote: ... Two million NIH-3T3 and K562 cells were collected and fixed in a 3 % glyoxal solution (40 % glyoxal (Merck, catalog no. 128465), 0.75 % acetic acid ...
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bioRxiv - Neuroscience 2023Quote: GNAO1+/G203R#2 cells at the stage of neuronal rosettes (30 day of differentiation) were fixed in 4% paraformaldehyde solution (Merck Life Sciences) for 15 min at room temperature and washed thrice with 1X PBS (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... total RNA was extracted (from TNBC cell lines or cryopreserved tumor tissue) using the GenElute mammalian total RNA miniprep kit (Sigma/Merck, RTN350-1KT) according to manufacturer’s instructions with an additional step of DNA digestion using the On-Column DNAse I digestion set according to manufacturer’s instructions (Sigma/Merck ...
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bioRxiv - Cancer Biology 2023Quote: ... DLD-1 cells expressing different EPHB1 wildtype or mutant versions and EFNB1 were mixed in suspension at a ratio of 1:3 and plated at a density of 130,000 cells/cm2 on coverslips coated with 2 mg/cm2 of 1-2 mg/mL laminin (Merck KGaA, Germany, USA) and incubated at 37ºC in 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated for 1 hour with 1% BSA blocking solution containing DAPI at 1:4,500 (Merck Life Sciences, Cat #: D9542), phalloidin at 1:350 (Merck Life Sciences ...
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bioRxiv - Cell Biology 2023Quote: ... a cell viability assay was performed after the luciferase assay by adding 12 μL of resazurin (Merck Life Sciences, Cat #: TOX8-1KT) and the cells incubated for an additional 2 hours followed by fluorescence reading on a Promega Glomax multimode plate reader.
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the cell lysates was measured using the BCA assay and insulin levels were quantified by radioimmunoassay (rat insulin RIA kit, Merck, Darmstadt, Germany).
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bioRxiv - Bioengineering 2023Quote: ... two thirds of the medium was removed from all wells and T cells were treated with 5 ng/mL polybrene (Merck, Darmstadt, Germany). Lentiviral particles (MOI=3 ...
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bioRxiv - Microbiology 2023Quote: ... The DNA content of the cells was analyzed using the ImageStreamX MarkII Quantitative imaging analysis for flow cytometry system (Merck Millipore, Germany). About 20,000 cells were collected for each sample and the data of the single cells were analyzed with the Flowjo software.
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bioRxiv - Microbiology 2023Quote: ... supernatant from the cells was collected and used to quantify LDH release using the LDH cytotoxicity detection kit (Roche, Merck, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: Human osteosarcoma SJSA-1 cells were purchased from ATCC (Manassas, VA, USA) and cultured with RPMI 1640 medium (Sigma Aldrich, Merck, Germany), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Genomics 2022Quote: 293T cells (#RCB2202, Riken BioResource Research Center, Tsukuba, Japan) were cultured in Dulbecco”s modified Eagle”s medium (#D5796, Merck, Darmstadt, Germany) supplemented with 10% heat-inactivated fetal calf serum (#10270106 ...
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bioRxiv - Immunology 2023Quote: ... Lysate was clarified by spinning cells for 10 min at 20,000 × g before loading supernatant onto cOmplete™ His-Tag purification resin (Merck; cat # 5893682001). Bound proteins were washed with 20 mM tris (pH 8.0) ...
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bioRxiv - Cell Biology 2023Quote: ... HUVECs were seeded at 5,000-10,000 cells per well in 8-well Lab-Tek® chamber slides (Nunc®, Merck, Dorset, UK) and grown to 60-70% confluence ...
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bioRxiv - Microbiology 2023Quote: ... inhibens were prepared through the following steps: Algal cell concentrations from a late exponential phase culture were enumerated using a CellStream CS-100496 flow cytometer (Merck, Darmstadt, Germany) with 561 nm excitation and 702 nm emission ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were transfected with the pCBASce I vector (expression vector of I-Sce I endonuclease) using Nano Juice (Merck, NJ, USA). Cells were harvested and suspended in PBS containing 1 mM EDTA 48 h after pCBASce I transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... Viral particles were harvested from cells four days after transfection and concentrated using 50kDa Amicon Ultra-15 Centrifugal Filter Units (Merck, cat#UFC905024). Organoids were dissociated by mechanical disruption and dissolved in 250 μL ENR medium supplemented with Y-27632 and Polybrene together with the concentrated virus ...
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bioRxiv - Developmental Biology 2023Quote: The culture of cells was conducted using filtered media that were covered with a layer of paraffin oil (Merck Group, Darmstadt, Germany). Prior to use ...
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bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 3.0 µg mL-1 α-factor grown at 25°C (Merck, Cat. No. T6901). After 90 min ...
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bioRxiv - Plant Biology 2024Quote: Visualization of intracellular and apoplastic ROS in guard cells was performed using 20 µM H2DCFDA or 100 µM peroxy orange 1 for H2O2 (PO1; Merck Sigma-Aldrich), and 100 µg/ml Oxyburst Green H2HFF-BSA (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... The stock solution was added at 1% v/v to Plasmax cell culture medium (CancerTools cat# 156371) supplemented with 5% FBS (Merck cat# F7524) and penicillin/streptomycin (Gibco cat# 15070063) ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were resuspended in 1 mL PBS containing protease inhibitor cocktail (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck, #11836153001) (PBSI ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was retained and centrifuged at 2000×g to remove remaining cell debris before being concentrated using 100kDa Amicon Ultra-15 centrifugal filters (Merck Millipore, USA), centrifuged at 3400×g to a final volume of 1mL ...
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bioRxiv - Cancer Biology 2021Quote: ... Percentage of GFP- or mCherry-positive cells was measured every three days from day 3 to day 18 post-infection using Guava® easyCyte™ Flow Cytometer (Merck Millipore). The percentage of fluorescent cells in the population on each day was divided by their percentage on day three to calculate fold-change corresponding to the effect of a given sgRNA on cell proliferation.
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bioRxiv - Microbiology 2021Quote: ... the cell layers were washed again with PBS and then lysed for 10 min with PBS containing 1% Elugent (Merck Millipore, Darmstadt, Germany) and 0,0625% Antifoam B (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2019Quote: The detection of apoptotic cells was based on the nick-end (TUNEL) labelling method using the ApopTag® in situ detection kit (S7100, Merck Millipore, USA), according to the manufacturing protocol.
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bioRxiv - Systems Biology 2021Quote: ... 1 hour prior to the infection the cell culture media containing the different drugs was replaced with media containing 80 µM cholesterol (Sigma-Aldrich, Merck KGaA, Germany). After the 1-hour-long cholesterol treatment the cells were infected with SARS-CoV-2 (GISAID accession ID ...
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bioRxiv - Biophysics 2020Quote: ... functionalized with either 100 μg/mL poly-D-lysine overnight for microglia cells or with 0.2 mg/mL fibronectin (FC010, Merck, 1:5 in PBS) for 2h at 37°C for fibroblasts ...
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bioRxiv - Cell Biology 2021Quote: ... 1.5E05 cells were seeded onto a T25 Matrigel coated flask in StemFlex medium supplemented with 10 µM rock inhibitor (Merck Millipore, Y-27632). Medium was refreshed the following day without rock inhibitor and lived cells were sent over to Cell Line Genetics when 20-30% confluence was reached ...
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bioRxiv - Cell Biology 2021Quote: ... were applied for one hour at room temperature and cells were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; Merck G8294; 1:3000) before mounting with Fluoromount™.
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bioRxiv - Neuroscience 2020Quote: Different cellular and mitochondrial parameters of the glutamate- or erastin-induced cell death pathways were analyzed using the Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany) upon harvesting adherent HT22 cells and following addition of different fluorescent dyes.
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bioRxiv - Cell Biology 2022Quote: ... shMYO10 #3 and shMYO10 #4 cell lines were generated using lentiviruses particles containing a non-target control shRNA (Merck, Cat Number: SHC016V-1EA) or shRNA targeting human MYO10 respectively (shMYO10 #3 ...
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bioRxiv - Immunology 2022Quote: ... the cells were transferred to a single well containing a monolayer of mitomycin C (Cat no. 50-07-7, Millipore Sigma, Merck, Darmstadt, Germany)-treated OP9/ OP9-DL1 cells or were kept unaltered ...
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bioRxiv - Biophysics 2022Quote: ... coli cells were grown in M9 medium (Green et al., 2012; Meyer and Schlegel, 1983) containing (15NH4)2SO4 (Sigma/Merck KGaA, Darmstadt, Germany) or (15NH4)2SO4 and 13C-D-glucose (Euriso-Top GmbH ...
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bioRxiv - Cancer Biology 2022Quote: ... Target cells (MDA, A549 and HCT) were then infected with the produced virus using 2μg/ml polybrene (Merck, Darmstadt, Germany, #TR-1003-G). Stably transduced cells were either selected by flow cytometry using BD FACSAria III cell sorter or 1μg/ml puromycin (InvivoGen ...
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bioRxiv - Genomics 2019Quote: Cells were seeded at a density of 4 × 104 cells per well in laminin coated 4 well chamber slides (Millicell® EZ slide, Merck Millipore, PEZGS0816). Cells were washed in cold PBS and fixed using 4% paraformaldehyde (PFA) ...
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bioRxiv - Biophysics 2020Quote: ... Primary antibodies used to image centrioles in expanded RPE-1 cells were rabbit anti-polyglutamate chain (polyE), pAb (IN105, 1:250) and mouse monoclonal anti-acetylated tubulin (Merck, T7451, 1:500). Secondary antibodies were goat anti-Rabbit Alexa488 (Thermo-Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... The percentage of COX-2 positive cells was quantified on 5.103 dissociated UC-hMSCs using a Guava® easyCyte Flow Cytometer (Merck Millipore, Guyancourt, France). The results were compared to the fluorescent intensity distribution obtained by image analysis.
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; GAPDH: #5174, Cell Signaling and GFAP #MAB360, Merck Millipore, Germany). After incubation with the first antibody ...
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bioRxiv - Microbiology 2020Quote: ... the bacteria were inoculated into the same medium (0.2% v/v) and the cell density was monitored using a Guava EasyCyte flow cytometer before harvesting (Merck Millipore, Billerica, MA, USA). Cell pellets were harvested from 10 ml culture by centrifugation which were stored at −80°C prior to lipid extraction.
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bioRxiv - Genetics 2021Quote: ... Cells were differentiated by LIF withdrawal in DMEM supplemented with 10% FBS and 0.1 mM ß-Mercaptoethanol at a density of 2×104 cells/cm2 on Fibronectin-coated dishes (Merck, 10 µg/ml).
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bioRxiv - Developmental Biology 2021Quote: We mated Exosc10+/+ or Exosc10+/− females with stud Exosc10+/− male mice and recovered two-cell stage (gestational day 2; GD2) embryos by flushing approximately 0.5 ml of EmbryoMax Advanced KSOM medium (Merck-Sigma, MR-101-D) into the oviduct ...
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bioRxiv - Microbiology 2021Quote: ... Five milliliters of each standard solution was placed in a flat-bottom test tube for spectrophotometer analysis (Spectroquant Empty cells 16 mm; Merck KGaA, Darmstadt, Germany), which was also used for incubation ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse neurons were plated at a density of 13 to 19 x103 cells/cm2 on glass coverslips coated with 333 µg/mL PDL and 100 to 200 µg/mL laminin (Merck/Sigma-Aldrich #L2020). For biochemical experiments ...
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bioRxiv - Neuroscience 2023Quote: Cerebellar organoids were generated as previously described (Silva et al 2020) with some alterations: 80-90% confluent iPSCs were dissociated into single cells using Accutase (Merck, Cat. no. A6964), and 4,500 cells were seeded per well of 96 well V-bottom low adhesion plates (S-bio ...